Rh4DG414700

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004435
Physical Location & Seq
Forward (+)
62496935 .. 62500070
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG414700.1

Sequence Viewer

Length: 1725 bp
ATGGATCTCCTCCAAACCTACTCCGACGACCAAAACCCTAATTCCTCCCCGGAATCTTCGCCTCCGCGGCTCCTCCCACCCAAGTCCGCCGCGCCCCAGGTCGACGACACCATGCTGGCCCTGTCGGTGGCCAAGAACCGCAGCGTATTGTCCCGGCCCATCGACCCGACCCAGCACCTCGTCGACTTCAACCCGACCTACGACCAGCTCTGGGCCCCAATCTACGGCCCATCTCACCCTTACGCCAAGGACGGCCTCGCCCAGGGCATGCGCAACCACAAGCTAGGGTTCGTCGAGGACGCCGCCATCGAGCCCTTCATCTTCGACGAGCAGTACAACACTTTCCAGAACTACGGCTACGCCGCCGACCCCTCCGCCTCCGCCGGCTACAATTACGTTGGCGATTTGGCCGCCTTGCAGAAAAACGACGCCGTTTCTGTGTACAACATCCCCCAGCACGAGCAGAAGAAGCGGAAGATTGAGAAGAGGAAGGAGAACGAGGATGGTGAAGAAGACGCCGAGATGGACCCGGAGGAGGTGGCGAACCCGGCCAGCGACGCGTGGCTGATGAAGAATAAGAAGAGCCCATGGGCCGGGAAGAAGGAGGGTTTGCCGGCGGAGCTGACGGAGGAGCAGAAGAAATATGCAGAGGAATATGCGAAGAAGAAAGGTGAAGAGAAAGGTGCCGGCGACAAGGGAGAGGCTGTGGTTGAGAAGACTACTTTCCACGGCAAAGAAGAGAGAGACTATCAAGGAAGGTCTTGGATTGCGCCGCCGAGAGATGCCAAGGCTACTAATGATCACTGTTACATTCCGAAACGGCTTGTGCATACTTGGAGTGGTCACACCAAGGGTGTGTCTGCTATTAGGTTCTTCCCGAATTCGGGGCATTTGATTCTCTCTGCAGGGCTGGATACTAAGGTGAAGATTTGGGATGTGTTCAACACTGGTAAGTGTATGAGAACTTACATGGGGCATTCGAAGGGTGTTAGGGATATTTGTTTTTCGAATGATGGGAGTAAGTTTTTGAGTGCTGGTTATGATAAGAATATCAAGTATTGGGATACGGAAACTGGGCAGGTGATATCTACATTTTCGACTGGGAAGGTTCCGTATGTGGTGAAGCTTAATCCGGATGAGGATAAGCAGAATGTGTTGTTGGCTGGGATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATGAGGGGAAGATCACTCAAGAGTATGATCAGCATTTGGGTGCGGTGAATACTATTACATTTGTTGATAATAATCGGAGGTTTGTTACATCCAGTGATGACAAGTCTCTTCGTGTATGGGAGTTTGGGATTCCTGTGGTTATCAAGTATATTAGTGAGCCTCACATGCATTCTATGCCATCAATTTCGTTGCACCCCAATTCGAATTGGCTTGCAGCACAAAGTATGGACAACCAGATTCTTATTTACAGCACTAGGGAGAAGTTTCAGCTCAATAAGAAGAAGAGGTTTGCAGGACATGTTGTTGCGGGGTATGCTTGCCAAGTGAATTTCTCGCCTGATGGACGGTTTGTTTTCTCGGGTGATGGTGAAGGTCGATGCTGGTTTTGGGATTGGAAGACTTGCAAAGTCTTCAAAACTCTCAGATGTCATGATGGGGTGTGCATTGGGGCAGCTTGGCATCCCTTGGAGCAAAGTAAAGTCGCAACTTGTGGCTGGGATGGCTTGATTAAATATTGGGACTAG

Protein Analysis

574

Amino Acids

64.81

Weight (kDa)

6.4

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 274 - 312 5.5e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 275 - 380 1.5e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 275 - 440 2.2e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 275 - 368 9.1e-17 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 277 - 441 4.2e-11 TEP-1 second beta-propeller
WD40_Gbeta PF25391 278 - 347 5.1e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 279 - 573 9.1e-54 WDR5 beta-propeller domain
EIF3I PF24805 280 - 382 1.5e-11 EIF3I
Beta-prop_WDR3_2nd PF25172 281 - 366 3.1e-10 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 281 - 485 5.3e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 282 - 367 1.1e-11 Prp19 WD40 domain
WDR55 PF24796 285 - 442 1e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 315 - 381 6.6e-10 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 317 - 355 5.6e-10 WD domain, G-beta repeat
Beta-prop_WDR41 PF25178 325 - 446 9.8e-07 WDR41
WD40_RFWD3 PF23419 326 - 401 3.7e-07 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2 PF23414 340 - 441 9.9e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 343 - 441 8.2e-06 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 346 - 441 3.4e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 357 - 442 5.3e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 373 - 547 1.1e-22 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 381 - 484 8.4e-12 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 382 - 568 4.7e-15 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 384 - 490 1.6e-07 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 384 - 545 5.1e-08 EIF3I
Beta-prop_THOC3 PF25174 385 - 574 6.4e-23 THOC3 beta-propeller domain
WDR90_beta-prop_4th PF23342 416 - 550 1e-05 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 453 - 574 9.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 455 - 574 4.8e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 457 - 539 6e-08 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 460 - 574 3.2e-08 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1069
Acc16I TGCGCA 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 683
AccI GTMKAC 2 cut(s) 102, 183
AccII CGCG 3 cut(s) 67, 92, 560
AccIII TCCGGA 1 cut(s) 1132
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 3 cut(s) 129, 408, 549
AcsI RAATTY 2 cut(s) 880, 1528
AcyI GRCGYC 3 cut(s) 300, 429, 516
AfaI GTAC 2 cut(s) 335, 443
AfiI CCNNNNNNNGG 8 cut(s) 127, 211, 224, 262, 535, 593, 883, 884
AflIII ACRYGT 2 cut(s) 558, 1498
AgsI TTSAA 3 cut(s) 190, 943, 1615
AjnI CCWGG 2 cut(s) 96, 261
AjuI GAANNNNNNNTTGG 2 cut(s) 1142, 1174
AluBI AGCT 6 cut(s) 208, 283, 622, 1126, 1471, 1655
AluI AGCT 6 cut(s) 208, 283, 622, 1126, 1471, 1655
Alw26I GTCTC 2 cut(s) 738, 1311
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 1558
Aor13HI TCCGGA 1 cut(s) 1132
AoxI GGCC 9 cut(s) 117, 129, 155, 213, 226, 253, 408, 549, 591
ApaI GGGCCC 1 cut(s) 217
ApeKI GCWGC 3 cut(s) 141, 1415, 1652
ApoI RAATTY 2 cut(s) 880, 1528
AspLEI GCGC 3 cut(s) 94, 273, 772
AspS9I GGNCC 7 cut(s) 118, 156, 213, 214, 227, 526, 591
AsuC2I CCSGG 5 cut(s) 50, 154, 530, 548, 595
AsuHPI GGTGA 9 cut(s) 227, 518, 683, 934, 1093, 1132, 1258, 1574, 1580
AsuII TTCGAA 3 cut(s) 980, 1007, 1403
AvaI CYCGRG 1 cut(s) 1558
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 217
BaeI ACNNNNGTAYC 2 cut(s) 906, 939
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 3 cut(s) 217, 315, 587
BauI CACGAG 1 cut(s) 458
BbsI GAAGAC 4 cut(s) 519, 722, 1603, 1604
BbvI GCAGC 3 cut(s) 153, 1427, 1664
BceAI ACGGC 6 cut(s) 241, 268, 370, 416, 745, 836
BciT130I CCWGG 2 cut(s) 98, 263
BciVI GTATCC 2 cut(s) 907, 1057
BclI TGATCA 2 cut(s) 799, 1228
BcnI CCSGG 5 cut(s) 50, 154, 530, 548, 595
BcoDI GTCTC 2 cut(s) 738, 1311
BfaI CTAG 3 cut(s) 284, 1455, 1723
BfmI CTRYAG 1 cut(s) 903
BfuAI ACCTGC 1 cut(s) 1069
BfuI GTATCC 2 cut(s) 907, 1057
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 9 cut(s) 68, 90, 142, 303, 363, 411, 773, 1416, 1653
BlsI GCNGC 9 cut(s) 69, 91, 143, 304, 364, 412, 774, 1417, 1654
Bme1390I CCNGG 7 cut(s) 50, 98, 154, 263, 530, 548, 595
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 1558
BmgT120I GGNCC 7 cut(s) 118, 156, 213, 214, 227, 526, 591
BmiI GGNNCC 6 cut(s) 71, 215, 216, 528, 685, 1110
BmrFI CCNGG 7 cut(s) 50, 98, 154, 263, 530, 548, 595
BmrI ACTGGG 2 cut(s) 1083, 1110
BmsI GCATC 3 cut(s) 772, 1568, 1669
BmuI ACTGGG 2 cut(s) 1083, 1110
BpiI GAAGAC 4 cut(s) 519, 722, 1603, 1604
Bpu14I TTCGAA 3 cut(s) 980, 1007, 1403
BpuEI CTTGAG 1 cut(s) 1203
BpuMI CCSGG 5 cut(s) 50, 154, 530, 548, 595
BsaBI GATNNNNATC 1 cut(s) 1272
BsaHI GRCGYC 3 cut(s) 300, 429, 516
BsaWI WCCGGW 1 cut(s) 1132
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 8 cut(s) 127, 211, 224, 262, 535, 593, 883, 884
Bse118I RCCGGY 3 cut(s) 383, 613, 686
Bse1I ACTGG 4 cut(s) 952, 1078, 1105, 1293
Bse8I GATNNNNATC 1 cut(s) 1272
BseAI TCCGGA 1 cut(s) 1132
BseBI CCWGG 2 cut(s) 98, 263
BseGI GGATG 8 cut(s) 447, 508, 940, 1141, 1173, 1289, 1660, 1705
BseJI GATNNNNATC 1 cut(s) 1272
BseLI CCNNNNNNNGG 8 cut(s) 127, 211, 224, 262, 535, 593, 883, 884
BseMII CTCAG 1 cut(s) 1636
BseNI ACTGG 4 cut(s) 952, 1078, 1105, 1293
BseRI GAGGAG 3 cut(s) 62, 548, 644
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 3 cut(s) 153, 1427, 1664
BseYI CCCAGC 4 cut(s) 171, 453, 1163, 1695
Bsh1236I CGCG 3 cut(s) 67, 92, 560
BshFI GGCC 9 cut(s) 119, 131, 157, 215, 228, 255, 410, 551, 593
BshNI GGYRCC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 1558
BsiSI CCGG 9 cut(s) 50, 154, 384, 530, 548, 594, 614, 687, 1133
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 8 cut(s) 127, 211, 224, 262, 535, 593, 883, 884
BsmAI GTCTC 2 cut(s) 738, 1311
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 2 cut(s) 976, 1369
BsnI GGCC 9 cut(s) 119, 131, 157, 215, 228, 255, 410, 551, 593
BsoBI CYCGRG 1 cut(s) 1558
Bsp119I TTCGAA 3 cut(s) 980, 1007, 1403
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 3 cut(s) 217, 315, 587
Bsp13I TCCGGA 1 cut(s) 1132
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 4 cut(s) 4, 799, 1212, 1228
Bsp19I CCATGG 1 cut(s) 587
BspANI GGCC 9 cut(s) 119, 131, 157, 215, 228, 255, 410, 551, 593
BspCNI CTCAG 1 cut(s) 1635
BspEI TCCGGA 1 cut(s) 1132
BspFNI CGCG 3 cut(s) 67, 92, 560
BspHI TCATGA 1 cut(s) 1630
BspLI GGNNCC 6 cut(s) 71, 215, 216, 528, 685, 1110
BspMAI CTGCAG 1 cut(s) 907
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 575
BspT104I TTCGAA 3 cut(s) 980, 1007, 1403
BspT107I GGYRCC 1 cut(s) 683
BsrFI RCCGGY 3 cut(s) 383, 613, 686
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 4 cut(s) 952, 1078, 1105, 1293
BssAI RCCGGY 3 cut(s) 383, 613, 686
BssMI GATC 4 cut(s) 4, 799, 1212, 1228
BssNI GRCGYC 3 cut(s) 300, 429, 516
BssSI CACGAG 1 cut(s) 458
BssT1I CCWWGG 5 cut(s) 246, 587, 786, 849, 1665
Bst2BI CACGAG 1 cut(s) 458
Bst2UI CCWGG 2 cut(s) 98, 263
Bst4CI ACNGT 2 cut(s) 806, 1548
Bst6I CTCTTC 6 cut(s) 479, 575, 669, 732, 1314, 1478
BstACI GRCGYC 3 cut(s) 300, 429, 516
BstAPI GCANNNNNTGC 1 cut(s) 1375
BstAUI TGTACA 1 cut(s) 441
BstBI TTCGAA 3 cut(s) 980, 1007, 1403
BstC8I GCNNGC 8 cut(s) 117, 269, 385, 553, 615, 688, 1413, 1519
BstDEI CTNAG 2 cut(s) 918, 1622
BstDSI CCRYGG 3 cut(s) 65, 587, 727
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 8 cut(s) 447, 508, 940, 1141, 1173, 1289, 1660, 1705
BstFNI CGCG 3 cut(s) 67, 92, 560
BstHHI GCGC 3 cut(s) 94, 273, 772
BstKTI GATC 4 cut(s) 7, 802, 1215, 1231
BstMAI GTCTC 2 cut(s) 738, 1311
BstMBI GATC 4 cut(s) 4, 799, 1212, 1228
BstMWI GCNNNNNNNGC 9 cut(s) 67, 469, 548, 557, 619, 1366, 1375, 1514, 1701
BstNI CCWGG 2 cut(s) 98, 263
BstNSI RCATGY 3 cut(s) 271, 1369, 1502
BstSCI CCNGG 7 cut(s) 48, 96, 152, 261, 528, 546, 593
BstSFI CTRYAG 1 cut(s) 903
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 3 cut(s) 67, 92, 560
BstV1I GCAGC 3 cut(s) 153, 1427, 1664
BstV2I GAAGAC 4 cut(s) 519, 722, 1603, 1604
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 2 cut(s) 907, 1057
BsuRI GGCC 9 cut(s) 119, 131, 157, 215, 228, 255, 410, 551, 593
BtgI CCRYGG 3 cut(s) 65, 587, 727
BtsCI GGATG 8 cut(s) 447, 508, 940, 1141, 1173, 1289, 1660, 1705
BtsIMutI CAGTG 4 cut(s) 802, 945, 1196, 1300
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 8 cut(s) 117, 269, 385, 553, 615, 688, 1413, 1519
CciI TCATGA 1 cut(s) 1630
CfoI GCGC 3 cut(s) 94, 273, 772
Cfr10I RCCGGY 3 cut(s) 383, 613, 686
Cfr13I GGNCC 7 cut(s) 118, 156, 213, 214, 227, 526, 591
Cfr42I CCGCGG 1 cut(s) 68
CseI GACGC 4 cut(s) 308, 437, 524, 566
Csp6I GTAC 2 cut(s) 334, 442
CviAII CATG 7 cut(s) 112, 268, 588, 970, 1366, 1499, 1631
CviQI GTAC 2 cut(s) 334, 442
DdeI CTNAG 2 cut(s) 918, 1622
DpnI GATC 4 cut(s) 6, 801, 1214, 1230
DpnII GATC 4 cut(s) 4, 799, 1212, 1228
EaeI YGGCCR 3 cut(s) 129, 408, 549
Eam1104I CTCTTC 6 cut(s) 479, 575, 669, 732, 1314, 1478
EarI CTCTTC 6 cut(s) 479, 575, 669, 732, 1314, 1478
EciI GGCGGA 4 cut(s) 76, 364, 370, 632
Eco130I CCWWGG 5 cut(s) 246, 587, 786, 849, 1665
Eco24I GRGCYC 3 cut(s) 217, 315, 587
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 526
Eco88I CYCGRG 1 cut(s) 1558
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 96, 261
EcoRV GATATC 1 cut(s) 1086
EcoT14I CCWWGG 5 cut(s) 246, 587, 786, 849, 1665
EcoT22I ATGCAT 1 cut(s) 1371
EcoT38I GRGCYC 3 cut(s) 217, 315, 587
ErhI CCWWGG 5 cut(s) 246, 587, 786, 849, 1665
FaeI CATG 7 cut(s) 115, 271, 591, 973, 1369, 1502, 1634
FaqI GGGAC 1 cut(s) 136
FatI CATG 7 cut(s) 111, 267, 587, 969, 1365, 1498, 1630
FauI CCCGC 1 cut(s) 1501
FbaI TGATCA 2 cut(s) 799, 1228
FblI GTMKAC 2 cut(s) 102, 183
Fnu4HI GCNGC 9 cut(s) 68, 90, 142, 303, 363, 411, 773, 1416, 1653
FokI GGATG 8 cut(s) 434, 515, 947, 1148, 1180, 1276, 1647, 1712
FriOI GRGCYC 3 cut(s) 217, 315, 587
Fsp4HI GCNGC 9 cut(s) 68, 90, 142, 303, 363, 411, 773, 1416, 1653
FspBI CTAG 3 cut(s) 284, 1455, 1723
FspI TGCGCA 1 cut(s) 272
GlaI GCGC 3 cut(s) 93, 272, 771
GluI GCNGC 9 cut(s) 68, 90, 142, 303, 363, 411, 773, 1416, 1653
GsaI CCCAGC 4 cut(s) 175, 457, 1167, 1699
HaeIII GGCC 9 cut(s) 119, 131, 157, 215, 228, 255, 410, 551, 593
HapII CCGG 9 cut(s) 50, 154, 384, 530, 548, 594, 614, 687, 1133
HgaI GACGC 4 cut(s) 308, 437, 524, 566
HhaI GCGC 3 cut(s) 94, 273, 772
Hin1I GRCGYC 3 cut(s) 300, 429, 516
Hin1II CATG 7 cut(s) 115, 271, 591, 973, 1369, 1502, 1634
Hin6I GCGC 3 cut(s) 92, 271, 770
HinP1I GCGC 3 cut(s) 92, 271, 770
HincII GTYRAC 2 cut(s) 103, 184
HindII GTYRAC 2 cut(s) 103, 184
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 4 cut(s) 53, 895, 1330, 1438
HpaII CCGG 9 cut(s) 50, 154, 384, 530, 548, 594, 614, 687, 1133
HphI GGTGA 9 cut(s) 227, 518, 683, 934, 1093, 1132, 1258, 1574, 1580
Hpy166II GTNNAC 3 cut(s) 103, 184, 442
Hpy188I TCNGA 4 cut(s) 25, 816, 1278, 1625
Hpy188III TCNNGA 5 cut(s) 346, 877, 1133, 1220, 1631
Hpy8I GTNNAC 3 cut(s) 103, 184, 442
Hpy99I CGWCG 7 cut(s) 29, 107, 185, 296, 329, 431, 560
HpyAV CCTTC 7 cut(s) 325, 484, 595, 750, 976, 1099, 1565
HpyCH4III ACNGT 2 cut(s) 806, 1548
HpyCH4IV ACGT 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 469, 548, 557, 619, 1366, 1375, 1514, 1701
HpyF3I CTNAG 2 cut(s) 918, 1622
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 3 cut(s) 300, 429, 516
Hsp92II CATG 7 cut(s) 115, 271, 591, 973, 1369, 1502, 1634
HspAI GCGC 3 cut(s) 92, 271, 770
Kpn2I TCCGGA 1 cut(s) 1132
KroI GCCGGC 3 cut(s) 383, 613, 686
KroNI GCCGGC 3 cut(s) 385, 615, 688
Ksp22I TGATCA 2 cut(s) 799, 1228
KspI CCGCGG 1 cut(s) 68
Kzo9I GATC 4 cut(s) 4, 799, 1212, 1228
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 4 cut(s) 75, 619, 631, 1669
Lsp1109I GCAGC 3 cut(s) 153, 1427, 1664
LweI GCATC 3 cut(s) 772, 1568, 1669
MaeI CTAG 3 cut(s) 284, 1455, 1723
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 806, 842, 1285
MalI GATC 4 cut(s) 6, 801, 1214, 1230
MboI GATC 4 cut(s) 4, 799, 1212, 1228
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 217, 315, 587
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 7 cut(s) 40, 391, 880, 1383, 1399, 1405, 1528
MluI ACGCGT 1 cut(s) 558
MluNI TGGCCA 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 48
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 1371
MroI TCCGGA 1 cut(s) 1132
MroNI GCCGGC 3 cut(s) 383, 613, 686
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 1128, 1710
MslI CAYNNNNRTG 1 cut(s) 1239
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 9 cut(s) 50, 154, 384, 530, 548, 594, 614, 687, 1133
MspR9I CCNGG 7 cut(s) 50, 98, 154, 263, 530, 548, 595
Mva1269I GAATGC 2 cut(s) 976, 1369
MvaI CCWGG 2 cut(s) 98, 263
MvnI CGCG 3 cut(s) 67, 92, 560
MwoI GCNNNNNNNGC 9 cut(s) 67, 469, 548, 557, 619, 1366, 1375, 1514, 1701
NaeI GCCGGC 3 cut(s) 385, 615, 688
NciI CCSGG 5 cut(s) 50, 154, 530, 548, 595
NcoI CCATGG 1 cut(s) 587
NdeII GATC 4 cut(s) 4, 799, 1212, 1228
NgoMIV GCCGGC 3 cut(s) 383, 613, 686
NlaIII CATG 7 cut(s) 115, 271, 591, 973, 1369, 1502, 1634
NlaIV GGNNCC 6 cut(s) 71, 215, 216, 528, 685, 1110
NmeAIII GCCGAG 2 cut(s) 544, 801
NmuCI GTSAC 1 cut(s) 842
NsbI TGCGCA 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 1371
NspI RCATGY 3 cut(s) 271, 1369, 1502
NspV TTCGAA 3 cut(s) 980, 1007, 1403
PaeI GCATGC 1 cut(s) 271
PagI TCATGA 1 cut(s) 1630
PaqCI CACCTGC 1 cut(s) 1069
PasI CCCWGGG 1 cut(s) 262
PciI ACATGT 1 cut(s) 1498
PciSI GCTCTTC 1 cut(s) 575
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 2 cut(s) 976, 1369
PdiI GCCGGC 3 cut(s) 385, 615, 688
PfeI GAWTC 4 cut(s) 53, 895, 1330, 1438
PkrI GCNGC 9 cut(s) 69, 91, 143, 304, 364, 412, 774, 1417, 1654
PscI ACATGT 1 cut(s) 1498
Psp6I CCWGG 2 cut(s) 96, 261
PspFI CCCAGC 4 cut(s) 171, 453, 1163, 1695
PspGI CCWGG 2 cut(s) 96, 261
PspN4I GGNNCC 6 cut(s) 71, 215, 216, 528, 685, 1110
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 7 cut(s) 118, 156, 213, 214, 227, 526, 591
PsrI GAACNNNNNNTAC 2 cut(s) 341, 373
PstI CTGCAG 1 cut(s) 907
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 335, 443
RsaNI GTAC 2 cut(s) 334, 442
RseI CAYNNNNRTG 1 cut(s) 1239
SacII CCGCGG 1 cut(s) 68
SalI GTCGAC 2 cut(s) 101, 182
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 2 cut(s) 1128, 1710
SatI GCNGC 9 cut(s) 68, 90, 142, 303, 363, 411, 773, 1416, 1653
Sau3AI GATC 4 cut(s) 4, 799, 1212, 1228
Sau96I GGNCC 7 cut(s) 118, 156, 213, 214, 227, 526, 591
ScrFI CCNGG 7 cut(s) 50, 98, 154, 263, 530, 548, 595
SduI GDGCHC 3 cut(s) 217, 315, 587
SfaNI GCATC 3 cut(s) 772, 1568, 1669
SfcI CTRYAG 1 cut(s) 903
Sfr303I CCGCGG 1 cut(s) 68
SfuI TTCGAA 3 cut(s) 980, 1007, 1403
SgrBI CCGCGG 1 cut(s) 68
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 1 cut(s) 1218
SmoI CTYRAG 1 cut(s) 1218
SphI GCATGC 1 cut(s) 271
Sse9I AATT 7 cut(s) 40, 391, 880, 1383, 1399, 1405, 1528
SspI AATATT 1 cut(s) 1715
SspMI CTAG 3 cut(s) 284, 1455, 1723
StyD4I CCNGG 7 cut(s) 48, 96, 152, 261, 528, 546, 593
StyI CCWWGG 5 cut(s) 246, 587, 786, 849, 1665
TaaI ACNGT 2 cut(s) 806, 1548
TaiI ACGT 1 cut(s) 399
TasI AATT 7 cut(s) 40, 391, 880, 1383, 1399, 1405, 1528
TatI WGTACW 2 cut(s) 333, 441
TauI GCSGC 6 cut(s) 70, 92, 305, 365, 413, 775
TfiI GAWTC 4 cut(s) 53, 895, 1330, 1438
Tru1I TTAA 2 cut(s) 1128, 1710
Tru9I TTAA 2 cut(s) 1128, 1710
TscAI CASTG 4 cut(s) 809, 952, 1196, 1300
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 3 cut(s) 141, 1415, 1652
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 3 cut(s) 307, 584, 1214
TspGWI ACGGA 3 cut(s) 641, 1082, 1101
TspRI CASTG 4 cut(s) 809, 952, 1196, 1300
VpaK11BI GGWCC 1 cut(s) 526
XagI CCTNNNNNAGG 1 cut(s) 260
XapI RAATTY 2 cut(s) 880, 1528
XceI RCATGY 3 cut(s) 271, 1369, 1502
XmiI GTMKAC 2 cut(s) 102, 183
XspI CTAG 3 cut(s) 284, 1455, 1723
Zsp2I ATGCAT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.