MD16G1092300.v1.1

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004435
Physical Location & Seq
Reverse (-)
6372852 .. 6376320
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1092300.v1.1.491

Sequence Viewer

Length: 1773 bp
ATGGATCTTCTCCGAGCATACTCTGATCAAAACGACGACGAATTGAACGGGCCTGAGGAGCAGAACCAAAACCCTAAATCCGCACCATCGTCCCCAGACGCGTCGCCGCCTCGCCTCCTCCCCACCAAGTCCGCCGCGCCCAATGTCGACGACACTATGCTGGCCCTGACGGTAGCCGGGGCCAACCGGTCTTCCACCCGCCGCATCGACCCGACCCAACACATCGTCGGCTTCAACCCAACCTACGAGCAGCTATGGGCACCAATCTACGGCCCGTCTCACCCTTACGCCAAGGACGGCATAGCCCAGGGGATGCGCAACCACAAGCTCGGCTTCGTCGAGGACGCCTCCATTGAGCCCTTCGTGTTCGACGAGCAGTACAACACTTTTCACAAGTACGGGTATGCTGCTGACCCTTCGGCATCTGCTGGGTACAATTATGTAGGTGATTTCGAGGCCTTACACAAGAATGACGCCGTTTCTGTGTATAACATTCCCCAGCACGAGCAGAAGAAGCGGAAGATTGAAAAGAGAAAGGAATTGGAGGAGAATGAGGGTGGGGATGAGGATATGGACCCGGAGGAGGTCCAGAACCCGGCTAGTGACGTGTGGTTGATGAAGAATAAGAAGAGTCCGTGGGCGGGAAAGAAGGAGGGTCTGCCGACGGAGCTGACTGAGGAGCAGAAGAAGTATGCCGAGGAGTATGCGAAGAAGAAAGGTGAGGAGAAAGGCGCTGGTGATAAGGGCGAGGCTGTGGCTGAGAAGACTACGTTTCATGGAAAAGAAGAGAGGGATTATCAGGGTAGGTCATGGATTGCGCCGCCGAGAGATGCTAAGGCATCTAATGATCACTGTTATATTCCGAAGAGGTTGGTGCACACTTGGAGCGGGCACACGAAGGGTGTGTCTGCTATTAGGTTCTTCCCTAAGTACGGGCATTTGATTCTGTCTGCCGGGATGGATACCAAGGTGAAGATTTGGGATGTTTTCAATACCGGTAAGTGTATGAGAACTTACATGGGTCACTCGAAGGCAGTTCGGGATATTTGTTTTTCTAATGATGGGAGTAGGTTCTTAACTGCTAGTTATGATAAGAATATCAAGTACTGGGATACAGAAACCGGGCAGGTGATATCTACATTCTCGACTGGGAAGGTTCCTTATGTGGTTAAGTTGAACCCGGATGAGGATAAGCAAAATGTGTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATGAGGGAAAGATCAATCAAGAGTATGATCAGCATTTGGGTGCTGTGAATACAATTACTTTTGTGGATAATAACCGTAGGTTTGTTACTTCCAGCGATGACAAGTCGCTTCGCGTATGGGAGTTTGGGATCCCTGTGGTTATTAAGTATATTAGTGAGCCTCACATGCATTCCATGCCATCAATTTCGCTGCACCCCAATTCCAATTGGCTTGCGGCACAGAGTATGGACAATCAGATTCTGATTTATAGCACTAGGGAGAAGTTTCAGCTCAATAAGAAGAAGAGGTTTGCCGGGCATGTCGTTGCCGGGTATGCTTGCCAAGTCAATTTCTCGCCAGATGGACGGTTTGTTATGTCAGGTGATGGTGAAGGTCGATGCTGGTTTTGGGATTGGAAGACATGCAAAGTCTTCAGAACTCTCAAATGTCACGAAGGGGTATGCATTGGGGCAGCATGGCATCCCTTGGAGCAAAGTAAAGTTGCAACATGTGGCTGGGATGGCTTAATCAAGTATTGGGACTAG

Protein Analysis

591

Amino Acids

66.83

Weight (kDa)

6.46

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 290 - 328 9.6e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 291 - 396 2.3e-18 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 291 - 457 6.8e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 291 - 384 1.5e-17 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 291 - 456 3.9e-16 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 292 - 448 1.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 292 - 381 1.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 294 - 366 6.3e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 295 - 589 3.4e-53 WDR5 beta-propeller domain
EIF3I PF24805 296 - 397 3.6e-13 EIF3I
WD40_Prp19 PF24814 298 - 383 1.4e-12 Prp19 WD40 domain
WDR55 PF24796 301 - 458 1.9e-17 WDR55
Beta-prop_WDR3_2nd PF25172 310 - 381 3.3e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 331 - 397 5.2e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 333 - 371 2.2e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 336 - 457 3.4e-10 TEP-1 second beta-propeller
WD40_RFWD3 PF23419 342 - 417 7.5e-08 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_WDR41 PF25178 352 - 462 8.1e-06 WDR41
WD40_Gbeta PF25391 355 - 458 1.4e-11 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 357 - 457 9.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 357 - 454 3.3e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 373 - 458 9.6e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 388 - 564 5e-23 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 397 - 500 1.6e-11 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 398 - 584 2e-15 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 399 - 501 9.5e-07 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 400 - 506 4.1e-07 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 401 - 562 5.7e-08 EIF3I
Beta-prop_THOC3 PF25174 401 - 590 5.8e-23 THOC3 beta-propeller domain
WDR90_beta-prop_4th PF23342 432 - 566 1.1e-06 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 469 - 590 8.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 471 - 590 2.3e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 473 - 554 2.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 476 - 590 4.5e-08 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc16I TGCGCA 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 888
AccI GTMKAC 1 cut(s) 147
AccII CGCG 3 cut(s) 101, 137, 1362
AclWI GGATC 3 cut(s) 12, 1372, 1385
AcuI CTGAAG 1 cut(s) 1645
AcyI GRCGYC 2 cut(s) 345, 474
AfaI GTAC 5 cut(s) 380, 398, 434, 932, 1106
AfiI CCNNNNNNNGG 6 cut(s) 269, 583, 595, 641, 932, 1186
AflIII ACRYGT 3 cut(s) 99, 606, 1736
AgeI ACCGGT 2 cut(s) 186, 995
AgsI TTSAA 5 cut(s) 46, 235, 527, 991, 1177
AjiI CACGTC 1 cut(s) 607
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 4 cut(s) 253, 328, 670, 1519
AluI AGCT 4 cut(s) 253, 328, 670, 1519
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 1 cut(s) 282
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 3 cut(s) 12, 1372, 1385
AlwNI CAGNNNCTG 1 cut(s) 1489
AoxI GGCC 5 cut(s) 50, 162, 180, 271, 456
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 4 cut(s) 250, 407, 1438, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
AsiGI ACCGGT 2 cut(s) 186, 995
AspLEI GCGC 4 cut(s) 139, 318, 734, 820
AspS9I GGNCC 6 cut(s) 50, 163, 180, 272, 574, 586
AsuC2I CCSGG 8 cut(s) 178, 578, 596, 955, 1123, 1181, 1543, 1558
AsuHPI GGTGA 8 cut(s) 272, 458, 731, 749, 982, 1141, 1622, 1628
AvaII GGWCC 2 cut(s) 574, 586
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 3 cut(s) 262, 879, 894
BaeI ACNNNNGTAYC 2 cut(s) 954, 987
BamHI GGATCC 1 cut(s) 1377
BanI GGYRCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 503
BbsI GAAGAC 4 cut(s) 183, 770, 1651, 1652
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 4 cut(s) 262, 394, 1425, 1712
BccI CCATC 7 cut(s) 94, 952, 1055, 1435, 1583, 1607, 1742
BceAI ACGGC 3 cut(s) 286, 313, 461
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 308
BciVI GTATCC 2 cut(s) 955, 1105
BclI TGATCA 3 cut(s) 25, 847, 1276
BcnI CCSGG 8 cut(s) 178, 578, 596, 955, 1123, 1181, 1543, 1558
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 4 cut(s) 600, 1083, 1503, 1771
BfoI RGCGCY 1 cut(s) 735
BfuAI ACCTGC 1 cut(s) 1117
BfuI GTATCC 2 cut(s) 955, 1105
BisI GCNGC 9 cut(s) 107, 135, 202, 251, 408, 821, 1439, 1464, 1701
BlsI GCNGC 9 cut(s) 108, 136, 203, 252, 409, 822, 1440, 1465, 1702
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 9 cut(s) 178, 308, 578, 596, 955, 1123, 1181, 1543, 1558
Bme18I GGWCC 2 cut(s) 574, 586
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 6 cut(s) 50, 163, 180, 272, 574, 586
BmiI GGNNCC 5 cut(s) 181, 261, 576, 1158, 1379
BmrFI CCNGG 9 cut(s) 178, 308, 578, 596, 955, 1123, 1181, 1543, 1558
BmrI ACTGGG 2 cut(s) 1117, 1158
BmsI GCATC 7 cut(s) 213, 303, 431, 820, 848, 1616, 1717
BmuI ACTGGG 2 cut(s) 1117, 1158
BpiI GAAGAC 4 cut(s) 183, 770, 1651, 1652
BplI GAGNNNNNCTC 2 cut(s) 332, 364
Bpu10I CCTNAGC 1 cut(s) 834
BpuMI CCSGG 8 cut(s) 178, 578, 596, 955, 1123, 1181, 1543, 1558
BsaHI GRCGYC 2 cut(s) 345, 474
BsaJI CCNNGG 8 cut(s) 177, 291, 306, 307, 635, 696, 966, 1713
BsaWI WCCGGW 2 cut(s) 186, 995
Bsc4I CCNNNNNNNGG 6 cut(s) 269, 583, 595, 641, 932, 1186
Bse118I RCCGGY 2 cut(s) 186, 995
Bse1I ACTGG 2 cut(s) 1112, 1153
Bse21I CCTNAGG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 8 cut(s) 177, 291, 306, 307, 635, 696, 966, 1713
BseGI GGATG 7 cut(s) 318, 568, 963, 988, 1189, 1708, 1753
BseLI CCNNNNNNNGG 6 cut(s) 269, 583, 595, 641, 932, 1186
BseMII CTCAG 3 cut(s) 45, 666, 750
BseNI ACTGG 2 cut(s) 1112, 1153
BseRI GAGGAG 7 cut(s) 71, 107, 560, 596, 692, 713, 737
BseSI GKGCMC 3 cut(s) 262, 879, 894
BseXI GCAGC 4 cut(s) 262, 394, 1425, 1712
BseYI CCCAGC 3 cut(s) 428, 498, 1743
BsgI GTGCAG 1 cut(s) 1424
Bsh1236I CGCG 3 cut(s) 101, 137, 1362
BshFI GGCC 5 cut(s) 52, 164, 182, 273, 458
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 2 cut(s) 186, 995
BsiHKAI GWGCWC 1 cut(s) 879
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 6 cut(s) 269, 583, 595, 641, 932, 1186
BsmAI GTCTC 1 cut(s) 282
BsmBI CGTCTC 1 cut(s) 282
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 1417
BsnI GGCC 5 cut(s) 52, 164, 182, 273, 458
Bsp1286I GDGCHC 4 cut(s) 262, 360, 879, 894
Bsp143I GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
BspANI GGCC 5 cut(s) 52, 164, 182, 273, 458
BspCNI CTCAG 3 cut(s) 46, 667, 751
BspFNI CGCG 3 cut(s) 101, 137, 1362
BspLI GGNNCC 5 cut(s) 181, 261, 576, 1158, 1379
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 3 cut(s) 12, 1372, 1385
BspT107I GGYRCC 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 888
BsrFI RCCGGY 2 cut(s) 186, 995
BsrI ACTGG 2 cut(s) 1112, 1153
BssAI RCCGGY 2 cut(s) 186, 995
BssECI CCNNGG 8 cut(s) 177, 291, 306, 307, 635, 696, 966, 1713
BssMI GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
BssNI GRCGYC 2 cut(s) 345, 474
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 3 cut(s) 291, 966, 1713
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 4 cut(s) 172, 854, 1325, 1596
Bst6I CTCTTC 4 cut(s) 623, 780, 860, 1526
BstACI GRCGYC 2 cut(s) 345, 474
BstAPI GCANNNNNTGC 1 cut(s) 1423
BstC8I GCNNGC 4 cut(s) 162, 890, 1461, 1567
BstDEI CTNAG 5 cut(s) 54, 675, 759, 834, 927
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 7 cut(s) 318, 568, 963, 988, 1189, 1708, 1753
BstFNI CGCG 3 cut(s) 101, 137, 1362
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 4 cut(s) 139, 318, 734, 820
BstKTI GATC 6 cut(s) 7, 28, 850, 1263, 1279, 1380
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
BstMWI GCNNNNNNNGC 7 cut(s) 58, 514, 667, 1414, 1423, 1562, 1749
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 4 cut(s) 1417, 1550, 1653, 1740
BstSCI CCNGG 9 cut(s) 176, 306, 576, 594, 953, 1121, 1179, 1541, 1556
BstSLI GKGCMC 3 cut(s) 262, 879, 894
BstUI CGCG 3 cut(s) 101, 137, 1362
BstV1I GCAGC 4 cut(s) 262, 394, 1425, 1712
BstV2I GAAGAC 4 cut(s) 183, 770, 1651, 1652
BstX2I RGATCY 2 cut(s) 4, 1377
BstYI RGATCY 2 cut(s) 4, 1377
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 2 cut(s) 955, 1105
BsuRI GGCC 5 cut(s) 52, 164, 182, 273, 458
BtgI CCRYGG 1 cut(s) 635
BtgZI GCGATG 1 cut(s) 1359
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 7 cut(s) 318, 568, 963, 988, 1189, 1708, 1753
BtsIMutI CAGTG 2 cut(s) 850, 1244
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 4 cut(s) 162, 890, 1461, 1567
CaiI CAGNNNCTG 1 cut(s) 1489
CfoI GCGC 4 cut(s) 139, 318, 734, 820
Cfr10I RCCGGY 2 cut(s) 186, 995
Cfr13I GGNCC 6 cut(s) 50, 163, 180, 272, 574, 586
CseI GACGC 4 cut(s) 90, 107, 353, 482
Csp6I GTAC 5 cut(s) 379, 397, 433, 931, 1105
CspAI ACCGGT 2 cut(s) 186, 995
CviAII CATG 9 cut(s) 776, 810, 1018, 1414, 1423, 1547, 1650, 1704, 1737
CviQI GTAC 5 cut(s) 379, 397, 433, 931, 1105
DdeI CTNAG 5 cut(s) 54, 675, 759, 834, 927
DpnI GATC 6 cut(s) 6, 27, 849, 1262, 1278, 1379
DpnII GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
Eam1104I CTCTTC 4 cut(s) 623, 780, 860, 1526
EarI CTCTTC 4 cut(s) 623, 780, 860, 1526
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 291, 966, 1713
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 360
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 2 cut(s) 574, 586
Eco57I CTGAAG 1 cut(s) 1645
Eco81I CCTNAGG 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 306
EcoRV GATATC 1 cut(s) 1134
EcoT14I CCWWGG 3 cut(s) 291, 966, 1713
EcoT22I ATGCAT 2 cut(s) 1419, 1694
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 3 cut(s) 291, 966, 1713
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 9 cut(s) 779, 813, 1021, 1417, 1426, 1550, 1653, 1707, 1740
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 76
FatI CATG 9 cut(s) 775, 809, 1017, 1413, 1422, 1546, 1649, 1703, 1736
FauI CCCGC 3 cut(s) 206, 634, 881
FbaI TGATCA 3 cut(s) 25, 847, 1276
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 9 cut(s) 107, 135, 202, 251, 408, 821, 1439, 1464, 1701
FokI GGATG 7 cut(s) 325, 575, 970, 995, 1196, 1695, 1760
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 9 cut(s) 107, 135, 202, 251, 408, 821, 1439, 1464, 1701
FspBI CTAG 4 cut(s) 600, 1083, 1503, 1771
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 4 cut(s) 138, 317, 733, 819
GluI GCNGC 9 cut(s) 107, 135, 202, 251, 408, 821, 1439, 1464, 1701
GsaI CCCAGC 3 cut(s) 432, 502, 1747
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 5 cut(s) 52, 164, 182, 273, 458
HgaI GACGC 4 cut(s) 90, 107, 353, 482
HhaI GCGC 4 cut(s) 139, 318, 734, 820
Hin1I GRCGYC 2 cut(s) 345, 474
Hin1II CATG 9 cut(s) 779, 813, 1021, 1417, 1426, 1550, 1653, 1707, 1740
Hin6I GCGC 4 cut(s) 137, 316, 732, 818
HinP1I GCGC 4 cut(s) 137, 316, 732, 818
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 3 cut(s) 631, 943, 1486
HphI GGTGA 8 cut(s) 272, 458, 731, 749, 982, 1141, 1622, 1628
Hpy166II GTNNAC 2 cut(s) 148, 877
Hpy188I TCNGA 6 cut(s) 14, 25, 864, 1485, 1491, 1664
Hpy188III TCNNGA 5 cut(s) 589, 1040, 1144, 1268, 1679
Hpy8I GTNNAC 2 cut(s) 148, 877
Hpy99I CGWCG 8 cut(s) 38, 41, 106, 152, 230, 341, 374, 667
HpyAV CCTTC 8 cut(s) 370, 426, 643, 892, 1024, 1147, 1613, 1676
HpyCH4III ACNGT 4 cut(s) 172, 854, 1325, 1596
HpyCH4IV ACGT 2 cut(s) 606, 770
HpyCH4V TGCA 6 cut(s) 877, 1417, 1441, 1653, 1692, 1733
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 514, 667, 1414, 1423, 1562, 1749
HpyF3I CTNAG 5 cut(s) 54, 675, 759, 834, 927
HpySE526I ACGT 2 cut(s) 606, 770
Hsp92I GRCGYC 2 cut(s) 345, 474
Hsp92II CATG 9 cut(s) 779, 813, 1021, 1417, 1426, 1550, 1653, 1707, 1740
HspAI GCGC 4 cut(s) 137, 316, 732, 818
Ksp22I TGATCA 3 cut(s) 25, 847, 1276
Kzo9I GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
LmnI GCTCC 5 cut(s) 58, 667, 679, 885, 1717
Lsp1109I GCAGC 4 cut(s) 262, 394, 1425, 1712
LweI GCATC 7 cut(s) 213, 303, 431, 820, 848, 1616, 1717
MaeI CTAG 4 cut(s) 600, 1083, 1503, 1771
MaeII ACGT 2 cut(s) 606, 770
MaeIII GTNAC 4 cut(s) 602, 1022, 1333, 1676
MalI GATC 6 cut(s) 6, 27, 849, 1262, 1278, 1379
MbiI CCGCTC 1 cut(s) 888
MboI GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
MfeI CAATTG 1 cut(s) 1453
MflI RGATCY 2 cut(s) 4, 1377
MhlI GDGCHC 4 cut(s) 262, 360, 879, 894
MluCI AATT 8 cut(s) 41, 436, 539, 1302, 1431, 1447, 1453, 1576
MluI ACGCGT 1 cut(s) 99
MlyI GAGTC 1 cut(s) 640
Mph1103I ATGCAT 2 cut(s) 1419, 1694
MseI TTAA 4 cut(s) 1076, 1170, 1392, 1754
MslI CAYNNNNRTG 2 cut(s) 468, 1287
MspR9I CCNGG 9 cut(s) 178, 308, 578, 596, 955, 1123, 1181, 1543, 1558
MunI CAATTG 1 cut(s) 1453
Mva1269I GAATGC 1 cut(s) 1417
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 3 cut(s) 101, 137, 1362
MwoI GCNNNNNNNGC 7 cut(s) 58, 514, 667, 1414, 1423, 1562, 1749
NciI CCSGG 8 cut(s) 178, 578, 596, 955, 1123, 1181, 1543, 1558
NdeII GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
NlaIII CATG 9 cut(s) 779, 813, 1021, 1417, 1426, 1550, 1653, 1707, 1740
NlaIV GGNNCC 5 cut(s) 181, 261, 576, 1158, 1379
NmeAIII GCCGAG 3 cut(s) 309, 721, 849
NmuCI GTSAC 3 cut(s) 602, 1022, 1676
NsbI TGCGCA 1 cut(s) 317
NsiI ATGCAT 2 cut(s) 1419, 1694
NspI RCATGY 4 cut(s) 1417, 1550, 1653, 1740
PaqCI CACCTGC 1 cut(s) 1117
PasI CCCWGGG 1 cut(s) 307
PceI AGGCCT 1 cut(s) 458
PciI ACATGT 1 cut(s) 1736
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 369
PctI GAATGC 1 cut(s) 1417
PfeI GAWTC 2 cut(s) 943, 1486
PinAI ACCGGT 2 cut(s) 186, 995
PkrI GCNGC 9 cut(s) 108, 136, 203, 252, 409, 822, 1440, 1465, 1702
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
PscI ACATGT 1 cut(s) 1736
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 3 cut(s) 428, 498, 1743
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 5 cut(s) 181, 261, 576, 1158, 1379
PspPI GGNCC 6 cut(s) 50, 163, 180, 272, 574, 586
PstNI CAGNNNCTG 1 cut(s) 1489
PsuI RGATCY 2 cut(s) 4, 1377
RsaI GTAC 5 cut(s) 380, 398, 434, 932, 1106
RsaNI GTAC 5 cut(s) 379, 397, 433, 931, 1105
RseI CAYNNNNRTG 2 cut(s) 468, 1287
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 4 cut(s) 1076, 1170, 1392, 1754
SatI GCNGC 9 cut(s) 107, 135, 202, 251, 408, 821, 1439, 1464, 1701
Sau3AI GATC 6 cut(s) 4, 25, 847, 1260, 1276, 1377
Sau96I GGNCC 6 cut(s) 50, 163, 180, 272, 574, 586
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 9 cut(s) 178, 308, 578, 596, 955, 1123, 1181, 1543, 1558
SduI GDGCHC 4 cut(s) 262, 360, 879, 894
SfaNI GCATC 7 cut(s) 213, 303, 431, 820, 848, 1616, 1717
SinI GGWCC 2 cut(s) 574, 586
SmiMI CAYNNNNRTG 2 cut(s) 468, 1287
Sse9I AATT 8 cut(s) 41, 436, 539, 1302, 1431, 1447, 1453, 1576
SseBI AGGCCT 1 cut(s) 458
SspMI CTAG 4 cut(s) 600, 1083, 1503, 1771
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 9 cut(s) 176, 306, 576, 594, 953, 1121, 1179, 1541, 1556
StyI CCWWGG 3 cut(s) 291, 966, 1713
TaaI ACNGT 4 cut(s) 172, 854, 1325, 1596
TaiI ACGT 2 cut(s) 609, 773
TaqI TCGA 8 cut(s) 147, 207, 339, 369, 453, 1028, 1145, 1624
TasI AATT 8 cut(s) 41, 436, 539, 1302, 1431, 1447, 1453, 1576
TatI WGTACW 2 cut(s) 378, 1104
TauI GCSGC 5 cut(s) 109, 137, 204, 823, 1466
TfiI GAWTC 2 cut(s) 943, 1486
Tru1I TTAA 4 cut(s) 1076, 1170, 1392, 1754
Tru9I TTAA 4 cut(s) 1076, 1170, 1392, 1754
TscAI CASTG 2 cut(s) 857, 1244
TseFI GTSAC 3 cut(s) 602, 1022, 1676
TseI GCWGC 4 cut(s) 250, 407, 1438, 1700
Tsp45I GTSAC 3 cut(s) 602, 1022, 1676
TspDTI ATGAA 3 cut(s) 632, 764, 1262
TspGWI ACGGA 2 cut(s) 624, 680
TspRI CASTG 2 cut(s) 857, 1244
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 574, 586
XceI RCATGY 4 cut(s) 1417, 1550, 1653, 1740
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 4 cut(s) 600, 1083, 1503, 1771
ZrmI AGTACT 1 cut(s) 1106
Zsp2I ATGCAT 2 cut(s) 1419, 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.