Rh5DG375700

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004435
Physical Location & Seq
Reverse (-)
57084612 .. 57086344
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG375700.1

Sequence Viewer

Length: 1587 bp
ATGGATCTTCTCCAAGCCTATTACGACCAACTGGACGATGATTTGGGCAGTTCAGAGGACCACAACCAAAACCCTAATTTCACATCGTCCCCAGAAGCTTCGCCACCAAGGCTCCTCCTCCAAGCCAAGTCCGCCGCACCACCCACGGTGGAAAAGACATTGTCAACGCCCATCGATCCCACCCAGCAGCACCTTGTCCACTTCAACCCTAACTTCTACGGCCCACAGCTGTCATGCATACGCAACCACAACCTTGGCTTCGTTGAAGACGCCTCCATCGAGCCCTTCGCCTTCCACGAGCAGTACAATACCTTCCAAACTTATGGCTACGCCGCCGATCCCTCCGCCTCTGACTTCGGCCATAGAGAGAACGACGCCGTTTCCATGTACAATGTACCGCAGCGCGAGCAGAAAAAGAAGCGCAAGATTGAAAAGAGCAAGGTGTCGGATGAGGGTTTGCCGGCTGAGCTAACGGAGGAGCAGAGGAAATACGCCGAGGAATATTATGCCAAGAAGAAAGGTGCACTGAGAGGCGGTGCCGGCGCCGACATGGGAGAGGCTATACTCGTCGAAAGAACTTGTTTCCATGGAAAAGAAGAGAGGGATTATCAGGGAAGCTCTTGGATAGCGCCGCCTAGTTACGCCAAGGCCACCAATGAGTACTGTTTTACACCCAAGAGGCTGGTCCACACTTGGAGTGGCCACACAAAGGGTGTGTCTGCTATTAGGTTCTACCCCAGATACGGGCATTTACTTCTCTCTGCAGGGATGGATAGTAGGGTCAAGATCTGGGACGTTTTCAATTACGGGAACTGCATGAGAACATACATGGGTCATTCCGAGGCAGTTAGGGACATTTGTTTCTCTAATGATGGCACTAAGTTTTTGAGCGCTTCTTTTGATAAGAATATCAAGTACTGGGATACAGAAACTGGCCAAGTCATATCCACCTTTTCGACCGGAAAAGTACCGTATGTGATTAAGCTTAATCCGGATGAGGATAAGCAGAATGTGTTATTGGCGGGGATGAGTGACAATAAGATTGTTCAGTGGGATGTGAACGAGGGGAAGAAGATCACTCACAAGTATGATCAGCATTTGCGTGCCGTGGATACCATTACGTTTGTTGATAATAACAGGAGGTTTGTTACTTCAAGTGATGACAAGTCTCTGCGTGTCTGGGAGTTTGGGATTCCTGTGGTGGTTAAGTGCATCAGTGAGCCTCACATGTATTCTATGCCTTCGGTTTCCTTGCACCCTAGTTCGAATTGGCTTGCGGCACAGAGCATGGATAACCAGATTCTTGTTTACAGCACTGGGGAGAAATTTCAGCAAGAAAAGAAGAAGCGGTTTGCAGGGCATGTTGTTGCAGGGCATGCTTGCCAAGTGAATTTCTCGCCAGATGGACGGTTTGTTTTCTCTGGTGACAGTGAAGGTCGATGCTGGTTTTGGGATTGGAAGTCTTGCAAGGTCTTGAAAACTCTCAAATGTCATCACGGGGTATGCATTGGGGCTGAGTGGCATCCCCTGGAGCAGAGTAAAATGGCAACGTGTGGCTGGGATGGCTTGATTAAGTATTGGGATTAG

Protein Analysis

528

Amino Acids

60.0

Weight (kDa)

6.47

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 226 - 321 5.9e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 226 - 320 1.7e-17 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 227 - 333 1.1e-17 THOC3 beta-propeller domain
WD40 PF00400 227 - 265 2.3e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 228 - 394 1.3e-16 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 229 - 319 1.9e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 231 - 302 7.5e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 232 - 527 9.3e-50 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 233 - 322 4.9e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 233 - 333 1.8e-13 EIF3I
WDR55 PF24796 239 - 396 1.4e-15 WDR55
Beta-prop_WDR3_2nd PF25172 248 - 319 3.1e-11 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 253 - 307 5.1e-06 MABP1/WDR62 second WD40 domain
WD40 PF00400 270 - 308 1.8e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 271 - 334 4.2e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 274 - 396 5.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 275 - 402 8.9e-07 WDR41
WD40_RFWD3 PF23419 279 - 359 1.1e-08 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_Gbeta PF25391 294 - 427 2.2e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 310 - 396 6.6e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 325 - 528 1.1e-20 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 332 - 438 1e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 335 - 522 4.2e-13 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 336 - 436 1e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 339 - 528 2.6e-22 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 380 - 528 1e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 414 - 528 1e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 415 - 494 1.6e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 415 - 528 9.8e-10 WDR55
WD40_CDC20-Fz PF24807 416 - 528 1.2e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 536, 542
AccII CGCG 1 cut(s) 405
AccIII TCCGGA 1 cut(s) 991
AclWI GGATC 3 cut(s) 12, 170, 332
AcoI YGGCCR 3 cut(s) 358, 700, 934
AcsI RAATTY 2 cut(s) 1325, 1390
AcyI GRCGYC 3 cut(s) 270, 375, 543
AfaI GTAC 6 cut(s) 305, 389, 396, 662, 917, 969
AfeI AGCGCT 1 cut(s) 892
AfiI CCNNNNNNNGG 3 cut(s) 709, 743, 744
AflIII ACRYGT 2 cut(s) 1227, 1550
AgsI TTSAA 6 cut(s) 205, 266, 431, 802, 1155, 1477
AjnI CCWGG 1 cut(s) 1527
AjuI GAANNNNNNNTTGG 2 cut(s) 1001, 1033
AluBI AGCT 5 cut(s) 98, 229, 469, 618, 985
AluI AGCT 5 cut(s) 98, 229, 469, 618, 985
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 1 cut(s) 1173
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 3 cut(s) 12, 170, 332
AlwNI CAGNNNCTG 1 cut(s) 932
Aor13HI TCCGGA 1 cut(s) 991
Aor51HI AGCGCT 1 cut(s) 892
AoxI GGCC 5 cut(s) 220, 358, 648, 700, 934
ApaLI GTGCAC 1 cut(s) 522
ApeKI GCWGC 2 cut(s) 187, 400
ApoI RAATTY 2 cut(s) 1325, 1390
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AspLEI GCGC 5 cut(s) 405, 423, 545, 631, 893
AspS9I GGNCC 3 cut(s) 58, 221, 685
AsuHPI GGTGA 1 cut(s) 1436
AsuII TTCGAA 1 cut(s) 1265
AvaII GGWCC 2 cut(s) 58, 685
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 2 cut(s) 702, 936
BanI GGYRCC 2 cut(s) 536, 542
BanII GRGCYC 1 cut(s) 285
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BauI CACGAG 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 2 cut(s) 199, 412
BccI CCATC 6 cut(s) 179, 284, 763, 866, 1397, 1556
BceAI ACGGC 3 cut(s) 235, 362, 1091
BciT130I CCWGG 1 cut(s) 1529
BciVI GTATCC 2 cut(s) 916, 1105
BclI TGATCA 1 cut(s) 1090
BcoDI GTCTC 1 cut(s) 1173
BfaI CTAG 2 cut(s) 636, 1260
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 3 cut(s) 546, 632, 894
BfuI GTATCC 2 cut(s) 916, 1105
BglI GCCNNNNNGGC 1 cut(s) 109
BglII AGATCT 1 cut(s) 786
BisI GCNGC 6 cut(s) 135, 188, 333, 401, 632, 1278
BlpI GCTNAGC 1 cut(s) 465
BlsI GCNGC 6 cut(s) 136, 189, 334, 402, 633, 1279
BmcAI AGTACT 2 cut(s) 662, 917
Bme1390I CCNGG 1 cut(s) 1529
Bme18I GGWCC 2 cut(s) 58, 685
BmgT120I GGNCC 3 cut(s) 58, 221, 685
BmiI GGNNCC 3 cut(s) 113, 538, 544
BmrFI CCNGG 1 cut(s) 1529
BmrI ACTGGG 2 cut(s) 928, 1326
BmsI GCATC 3 cut(s) 1221, 1430, 1531
BmuI ACTGGG 2 cut(s) 928, 1326
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 1 cut(s) 1550
Bpu1102I GCTNAGC 1 cut(s) 465
Bpu14I TTCGAA 1 cut(s) 1265
Bsa29I ATCGAT 1 cut(s) 174
BsaHI GRCGYC 3 cut(s) 270, 375, 543
BsaJI CCNNGG 9 cut(s) 107, 144, 253, 495, 586, 645, 840, 1107, 1527
BsaWI WCCGGW 2 cut(s) 959, 991
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 709, 743, 744
Bse118I RCCGGY 2 cut(s) 460, 539
Bse1I ACTGG 4 cut(s) 36, 923, 937, 1321
BseAI TCCGGA 1 cut(s) 991
BseBI CCWGG 1 cut(s) 1529
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 9 cut(s) 107, 144, 253, 495, 586, 645, 840, 1107, 1527
BseGI GGATG 7 cut(s) 454, 774, 1000, 1032, 1060, 1522, 1567
BseLI CCNNNNNNNGG 3 cut(s) 709, 743, 744
BseMII CTCAG 3 cut(s) 456, 518, 1506
BseNI ACTGG 4 cut(s) 36, 923, 937, 1321
BseRI GAGGAG 3 cut(s) 104, 107, 491
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 2 cut(s) 199, 412
BseYI CCCAGC 2 cut(s) 183, 1557
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 960
BshFI GGCC 5 cut(s) 222, 360, 650, 702, 936
BshNI GGYRCC 2 cut(s) 536, 542
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 960
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 4 cut(s) 461, 540, 960, 992
BslFI GGGAC 3 cut(s) 73, 806, 866
BslI CCNNNNNNNGG 3 cut(s) 709, 743, 744
BsmAI GTCTC 1 cut(s) 1173
BsmFI GGGAC 3 cut(s) 73, 806, 866
BsnI GGCC 5 cut(s) 222, 360, 650, 702, 936
Bsp119I TTCGAA 1 cut(s) 1265
Bsp1286I GDGCHC 2 cut(s) 285, 526
Bsp13I TCCGGA 1 cut(s) 991
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
Bsp1720I GCTNAGC 1 cut(s) 465
Bsp19I CCATGG 1 cut(s) 586
BspANI GGCC 5 cut(s) 222, 360, 650, 702, 936
BspCNI CTCAG 3 cut(s) 457, 519, 1507
BspDI ATCGAT 1 cut(s) 174
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 113, 538, 544
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 3 cut(s) 12, 170, 332
BspT104I TTCGAA 1 cut(s) 1265
BspT107I GGYRCC 2 cut(s) 536, 542
BsrFI RCCGGY 2 cut(s) 460, 539
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 4 cut(s) 36, 923, 937, 1321
BssAI RCCGGY 2 cut(s) 460, 539
BssECI CCNNGG 9 cut(s) 107, 144, 253, 495, 586, 645, 840, 1107, 1527
BssMI GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
BssNI GRCGYC 3 cut(s) 270, 375, 543
BssSI CACGAG 1 cut(s) 296
BssT1I CCWWGG 4 cut(s) 107, 253, 586, 645
Bst2BI CACGAG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 1529
Bst4CI ACNGT 5 cut(s) 148, 665, 972, 1410, 1430
Bst6I CTCTTC 1 cut(s) 591
BstACI GRCGYC 3 cut(s) 270, 375, 543
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstAUI TGTACA 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 1265
BstC8I GCNNGC 7 cut(s) 407, 462, 541, 1104, 1275, 1377, 1381
BstDEI CTNAG 4 cut(s) 465, 527, 879, 1515
BstDSI CCRYGG 3 cut(s) 144, 586, 1107
BstF5I GGATG 7 cut(s) 454, 774, 1000, 1032, 1060, 1522, 1567
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 3 cut(s) 546, 632, 894
BstHHI GCGC 5 cut(s) 405, 423, 545, 631, 893
BstKTI GATC 6 cut(s) 7, 178, 340, 789, 1077, 1093
BstMAI GTCTC 1 cut(s) 1173
BstMBI GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
BstMCI CGRYCG 1 cut(s) 960
BstMWI GCNNNNNNNGC 7 cut(s) 109, 131, 406, 466, 540, 1376, 1563
BstNI CCWGG 1 cut(s) 1529
BstNSI RCATGY 3 cut(s) 1231, 1364, 1379
BstSCI CCNGG 1 cut(s) 1527
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 199, 412
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 2 cut(s) 4, 786
BstXI CCANNNNNNTGG 3 cut(s) 254, 323, 682
BstYI RGATCY 2 cut(s) 4, 786
Bsu15I ATCGAT 1 cut(s) 174
BsuI GTATCC 2 cut(s) 916, 1105
BsuRI GGCC 5 cut(s) 222, 360, 650, 702, 936
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 3 cut(s) 144, 586, 1107
BtsCI GGATG 7 cut(s) 454, 774, 1000, 1032, 1060, 1522, 1567
BtsIMutI CAGTG 5 cut(s) 524, 1055, 1222, 1314, 1435
Cac8I GCNNGC 7 cut(s) 407, 462, 541, 1104, 1275, 1377, 1381
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 5 cut(s) 405, 423, 545, 631, 893
Cfr10I RCCGGY 2 cut(s) 460, 539
Cfr13I GGNCC 3 cut(s) 58, 221, 685
ClaI ATCGAT 1 cut(s) 174
CseI GACGC 2 cut(s) 278, 383
Csp6I GTAC 6 cut(s) 304, 388, 395, 661, 916, 968
CviQI GTAC 6 cut(s) 304, 388, 395, 661, 916, 968
DdeI CTNAG 4 cut(s) 465, 527, 879, 1515
DinI GGCGCC 1 cut(s) 544
DpnI GATC 6 cut(s) 6, 177, 339, 788, 1076, 1092
DpnII GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
EaeI YGGCCR 3 cut(s) 358, 700, 934
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
EciI GGCGGA 2 cut(s) 121, 334
Eco130I CCWWGG 4 cut(s) 107, 253, 586, 645
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 58, 685
Eco47III AGCGCT 1 cut(s) 892
EcoRII CCWGG 1 cut(s) 1527
EcoT14I CCWWGG 4 cut(s) 107, 253, 586, 645
EcoT22I ATGCAT 2 cut(s) 239, 1508
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 544
EheI GGCGCC 1 cut(s) 544
ErhI CCWWGG 4 cut(s) 107, 253, 586, 645
FaqI GGGAC 3 cut(s) 73, 806, 866
FauI CCCGC 1 cut(s) 1015
FbaI TGATCA 1 cut(s) 1090
Fnu4HI GCNGC 6 cut(s) 135, 188, 333, 401, 632, 1278
FokI GGATG 7 cut(s) 461, 781, 1007, 1039, 1067, 1509, 1574
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 6 cut(s) 135, 188, 333, 401, 632, 1278
FspBI CTAG 2 cut(s) 636, 1260
GlaI GCGC 5 cut(s) 404, 422, 544, 630, 892
GluI GCNGC 6 cut(s) 135, 188, 333, 401, 632, 1278
GsaI CCCAGC 2 cut(s) 187, 1561
GsuI CTGGAG 1 cut(s) 1550
HaeII RGCGCY 3 cut(s) 546, 632, 894
HaeIII GGCC 5 cut(s) 222, 360, 650, 702, 936
HapII CCGG 4 cut(s) 461, 540, 960, 992
HgaI GACGC 2 cut(s) 278, 383
HhaI GCGC 5 cut(s) 405, 423, 545, 631, 893
Hin1I GRCGYC 3 cut(s) 270, 375, 543
Hin6I GCGC 5 cut(s) 403, 421, 543, 629, 891
HinP1I GCGC 5 cut(s) 403, 421, 543, 629, 891
HincII GTYRAC 1 cut(s) 165
HindII GTYRAC 1 cut(s) 165
HindIII AAGCTT 2 cut(s) 96, 983
HinfI GANTC 2 cut(s) 1192, 1300
HpaII CCGG 4 cut(s) 461, 540, 960, 992
HphI GGTGA 1 cut(s) 1436
Hpy166II GTNNAC 6 cut(s) 165, 199, 524, 688, 1060, 1309
Hpy188I TCNGA 4 cut(s) 55, 352, 448, 841
Hpy188III TCNNGA 3 cut(s) 784, 992, 1474
Hpy8I GTNNAC 6 cut(s) 165, 199, 524, 688, 1060, 1309
Hpy99I CGWCG 2 cut(s) 377, 572
HpyAV CCTTC 5 cut(s) 295, 301, 322, 1251, 1427
HpyCH4III ACNGT 5 cut(s) 148, 665, 972, 1410, 1430
HpyCH4IV ACGT 3 cut(s) 795, 1121, 1550
HpyF10VI GCNNNNNNNGC 7 cut(s) 109, 131, 406, 466, 540, 1376, 1563
HpyF3I CTNAG 4 cut(s) 465, 527, 879, 1515
HpySE526I ACGT 3 cut(s) 795, 1121, 1550
Hsp92I GRCGYC 3 cut(s) 270, 375, 543
HspAI GCGC 5 cut(s) 403, 421, 543, 629, 891
KasI GGCGCC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 991
KroI GCCGGC 2 cut(s) 460, 539
KroNI GCCGGC 2 cut(s) 462, 541
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
LmnI GCTCC 3 cut(s) 117, 478, 1531
Lsp1109I GCAGC 2 cut(s) 199, 412
LweI GCATC 3 cut(s) 1221, 1430, 1531
MaeI CTAG 2 cut(s) 636, 1260
MaeII ACGT 3 cut(s) 795, 1121, 1550
MaeIII GTNAC 4 cut(s) 638, 1031, 1147, 1424
MalI GATC 6 cut(s) 6, 177, 339, 788, 1076, 1092
MboI GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
MboII GAAGA 6 cut(s) 278, 526, 608, 1081, 1084, 1354
MflI RGATCY 2 cut(s) 4, 786
MhlI GDGCHC 2 cut(s) 285, 526
MlsI TGGCCA 2 cut(s) 702, 936
MluCI AATT 5 cut(s) 76, 802, 1267, 1325, 1390
MluNI TGGCCA 2 cut(s) 702, 936
Mly113I GGCGCC 1 cut(s) 543
MmeI TCCRAC 1 cut(s) 426
Mox20I TGGCCA 2 cut(s) 702, 936
Mph1103I ATGCAT 2 cut(s) 239, 1508
MroI TCCGGA 1 cut(s) 991
MroNI GCCGGC 2 cut(s) 460, 539
MscI TGGCCA 2 cut(s) 702, 936
MseI TTAA 4 cut(s) 981, 987, 1206, 1572
MslI CAYNNNNRTG 2 cut(s) 1086, 1101
Msp20I TGGCCA 2 cut(s) 702, 936
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 4 cut(s) 461, 540, 960, 992
MspR9I CCNGG 1 cut(s) 1529
MvaI CCWGG 1 cut(s) 1529
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 7 cut(s) 109, 131, 406, 466, 540, 1376, 1563
NaeI GCCGGC 2 cut(s) 462, 541
NarI GGCGCC 1 cut(s) 543
NcoI CCATGG 1 cut(s) 586
NdeII GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
NgoMIV GCCGGC 2 cut(s) 460, 539
NlaIV GGNNCC 3 cut(s) 113, 538, 544
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 2 cut(s) 1031, 1424
NsiI ATGCAT 2 cut(s) 239, 1508
NspI RCATGY 3 cut(s) 1231, 1364, 1379
NspV TTCGAA 1 cut(s) 1265
PaeI GCATGC 1 cut(s) 1379
PciI ACATGT 1 cut(s) 1227
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 294
PdiI GCCGGC 2 cut(s) 462, 541
PfeI GAWTC 2 cut(s) 1192, 1300
PflFI GACNNNGTC 1 cut(s) 160
PkrI GCNGC 6 cut(s) 136, 189, 334, 402, 633, 1279
PluTI GGCGCC 1 cut(s) 546
PscI ACATGT 1 cut(s) 1227
Psp6I CCWGG 1 cut(s) 1527
PspFI CCCAGC 2 cut(s) 183, 1557
PspGI CCWGG 1 cut(s) 1527
PspN4I GGNNCC 3 cut(s) 113, 538, 544
PspPI GGNCC 3 cut(s) 58, 221, 685
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 2 cut(s) 4, 786
PsyI GACNNNGTC 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 6 cut(s) 305, 389, 396, 662, 917, 969
RsaNI GTAC 6 cut(s) 304, 388, 395, 661, 916, 968
RseI CAYNNNNRTG 2 cut(s) 1086, 1101
SaqAI TTAA 4 cut(s) 981, 987, 1206, 1572
SatI GCNGC 6 cut(s) 135, 188, 333, 401, 632, 1278
Sau3AI GATC 6 cut(s) 4, 175, 337, 786, 1074, 1090
Sau96I GGNCC 3 cut(s) 58, 221, 685
ScaI AGTACT 2 cut(s) 662, 917
ScrFI CCNGG 1 cut(s) 1529
SduI GDGCHC 2 cut(s) 285, 526
SfaNI GCATC 3 cut(s) 1221, 1430, 1531
SfcI CTRYAG 1 cut(s) 762
SfoI GGCGCC 1 cut(s) 544
SfuI TTCGAA 1 cut(s) 1265
SinI GGWCC 2 cut(s) 58, 685
SmiMI CAYNNNNRTG 2 cut(s) 1086, 1101
SphI GCATGC 1 cut(s) 1379
Sse9I AATT 5 cut(s) 76, 802, 1267, 1325, 1390
SspDI GGCGCC 1 cut(s) 542
SspI AATATT 1 cut(s) 503
SspMI CTAG 2 cut(s) 636, 1260
StyD4I CCNGG 1 cut(s) 1527
StyI CCWWGG 4 cut(s) 107, 253, 586, 645
TaaI ACNGT 5 cut(s) 148, 665, 972, 1410, 1430
TaiI ACGT 3 cut(s) 798, 1124, 1553
TaqI TCGA 6 cut(s) 174, 279, 570, 956, 1265, 1438
TasI AATT 5 cut(s) 76, 802, 1267, 1325, 1390
TatI WGTACW 4 cut(s) 303, 387, 660, 915
TauI GCSGC 4 cut(s) 137, 335, 634, 1280
TfiI GAWTC 2 cut(s) 1192, 1300
Tru1I TTAA 4 cut(s) 981, 987, 1206, 1572
Tru9I TTAA 4 cut(s) 981, 987, 1206, 1572
TscAI CASTG 5 cut(s) 531, 1055, 1222, 1321, 1435
TseFI GTSAC 2 cut(s) 1031, 1424
TseI GCWGC 2 cut(s) 187, 400
Tsp45I GTSAC 2 cut(s) 1031, 1424
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 5 cut(s) 531, 1055, 1222, 1321, 1435
Tth111I GACNNNGTC 1 cut(s) 160
VneI GTGCAC 1 cut(s) 522
VpaK11BI GGWCC 2 cut(s) 58, 685
XapI RAATTY 2 cut(s) 1325, 1390
XceI RCATGY 3 cut(s) 1231, 1364, 1379
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XspI CTAG 2 cut(s) 636, 1260
ZrmI AGTACT 2 cut(s) 662, 917
Zsp2I ATGCAT 2 cut(s) 239, 1508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.