FvH4_5g04970

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011698
Physical Location & Seq
Reverse (-)
2938622 .. 2941950
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04970.t1

Sequence Viewer

Length: 1155 bp
ATGGAGAACAACAACCAATCTTTCTGGCAGTTCAGTGACCAGCTTAGGGTGCAAACATCTAATCTCGCAAATCTTTCGGTCAACGATTCAATCTGGAGCAACTCATACGGCAGCAAGAGGCCTGATGCAAGGCGGAACTTCGATATCAAGGTCGGCGGTGAGGTGAACTCTCTGGTCAACTTGACTCCCAAAGGGTCTGATTTCAATGGTTTGGATTACAAGCAGAAAGGATCATCGGACCCGAACGAGGGATGGAACAGCTTCAAGCCCAAGGCTTCTGAGCTGAACGTGGTGAGTGATAGGTGGAACTCTTTCAAGCCGAAAGCTTCAGAGTTCAACTCTTTCAACCCGGATTTTAATGGGTTCAACCAGAAGGTCCCGAACTTTGATGGGTTTAACCAGAAGATGCCGGACTTTAATGGGTTCAATCAGAAGGTTCAGGACTTTAATGGGTTCAATGAAGGGTGGAAGCTTGGGGCTTCTGCTAATGGAGGCATCAATGGAGGCTTCAACAAGGGGATTTACTCCAAGCCTGCGATTCATAGCAATAATATTAACCTGAAGGCGTACAAGAACCAGGGGGAGGAGTTTGATCTGGGAGTTAAAGGTGGGAAGAAGAACAACAATAGCAACAAGAAGAAGAATGGTGATGACAATAACAAGGATGTTAAGGGTGACAAGAAATTCAAGACCCTTCCACCGTCTGAGTCTCTCCCTAGAAATGAGACCATTGGTGGTTACATCTTCGTCTGCAACAACGATACCATGCAAGAGAATCTCAAGAGGGAGCTCTTTGGTTTGCCTCCACGTTACCGTGACTCAGTCCGTGCCATTACTCCAGGGTTGCCCCTTTTCCTTTACAACTACTCCACTCACCAACTCCATGGAATTTTTGAGGCTGCAAGCTTTGGAGGAACCAACTTTGATCCATCTGCTTGGGAAGACAAGAAATGTCCTGGTGAATCGCGCTTCCCTGCTCAGGTGAAAGTTTTAACGAGGAAAGTCTGTGAACCACTGGAAGAGGATTCCTTCAGGCCAATTCTTCATCACTACGATGGCCCTAAATTCCGTCTTGAACTCAGCGTGCCAGAGGCTCTCTCTCTGTTGGACAAGTTTGCAGACCAAGATGATATATTTGTAAATGAAACCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

385

Amino Acids

43.22

Weight (kDa)

7.64

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 245 - 370 8.8e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 967
AciI CCGC 2 cut(s) 133, 156
AclWI GGATC 2 cut(s) 238, 920
AcsI RAATTY 3 cut(s) 683, 888, 1064
AcuI CTGAAG 3 cut(s) 312, 581, 1015
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 5 cut(s) 46, 247, 248, 583, 979
AjnI CCWGG 3 cut(s) 576, 838, 955
AluBI AGCT 7 cut(s) 43, 261, 283, 326, 472, 790, 906
AluI AGCT 7 cut(s) 43, 261, 283, 326, 472, 790, 906
Alw21I GWGCWC 1 cut(s) 792
Alw26I GTCTC 2 cut(s) 714, 719
AlwI GGATC 2 cut(s) 238, 920
AoxI GGCC 3 cut(s) 119, 1034, 1057
ApeKI GCWGC 2 cut(s) 111, 899
ApoI RAATTY 3 cut(s) 683, 888, 1064
Asp700I GAANNNNTTC 2 cut(s) 260, 311
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 3 cut(s) 238, 376, 1058
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 8 cut(s) 170, 175, 304, 659, 686, 866, 971, 994
AvaII GGWCC 2 cut(s) 238, 376
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 948
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 2 cut(s) 123, 886
BccI CCATC 4 cut(s) 246, 383, 937, 1049
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 3 cut(s) 578, 840, 957
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 714, 719
BfaI CTAG 1 cut(s) 717
BisI GCNGC 2 cut(s) 112, 900
BlsI GCNGC 2 cut(s) 113, 901
Bme1390I CCNGG 4 cut(s) 350, 578, 840, 957
Bme18I GGWCC 2 cut(s) 238, 376
BmgT120I GGNCC 3 cut(s) 238, 376, 1058
BmiI GGNNCC 3 cut(s) 240, 378, 916
BmrFI CCNGG 4 cut(s) 350, 578, 840, 957
BmsI GCATC 3 cut(s) 115, 396, 504
BpiI GAAGAC 1 cut(s) 948
BplI GAGNNNNNCTC 6 cut(s) 152, 184, 323, 355, 1082, 1114
BpmI CTGGAG 2 cut(s) 115, 822
Bpu10I CCTNAGC 2 cut(s) 44, 978
BpuEI CTTGAG 1 cut(s) 764
BpuMI CCSGG 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 4 cut(s) 270, 577, 839, 883
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 247, 248, 583, 979
Bse1I ACTGG 1 cut(s) 1020
BseBI CCWGG 3 cut(s) 578, 840, 957
BseDI CCNNGG 4 cut(s) 270, 577, 839, 883
BseGI GGATG 2 cut(s) 257, 670
BseLI CCNNNNNNNGG 5 cut(s) 46, 247, 248, 583, 979
BseMII CTCAG 5 cut(s) 270, 696, 834, 992, 1093
BseNI ACTGG 1 cut(s) 1020
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 2 cut(s) 123, 886
Bsh1236I CGCG 1 cut(s) 967
BshFI GGCC 3 cut(s) 121, 1036, 1059
BsiHKAI GWGCWC 1 cut(s) 792
BsiSI CCGG 2 cut(s) 350, 410
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 5 cut(s) 46, 247, 248, 583, 979
BsmAI GTCTC 2 cut(s) 714, 719
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 3 cut(s) 121, 1036, 1059
Bso31I GGTCTC 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 3 cut(s) 230, 592, 925
Bsp19I CCATGG 1 cut(s) 883
BspACI CCGC 2 cut(s) 133, 156
BspANI GGCC 3 cut(s) 121, 1036, 1059
BspCNI CTCAG 5 cut(s) 271, 697, 833, 991, 1092
BspFNI CGCG 1 cut(s) 967
BspLI GGNNCC 3 cut(s) 240, 378, 916
BspPI GGATC 2 cut(s) 238, 920
BspTNI GGTCTC 1 cut(s) 719
BsrI ACTGG 1 cut(s) 1020
BssECI CCNNGG 4 cut(s) 270, 577, 839, 883
BssMI GATC 3 cut(s) 230, 592, 925
BssT1I CCWWGG 2 cut(s) 270, 883
Bst2UI CCWGG 3 cut(s) 578, 840, 957
Bst4CI ACNGT 2 cut(s) 702, 815
Bst6I CTCTTC 1 cut(s) 1014
BstC8I GCNNGC 3 cut(s) 534, 904, 1085
BstDEI CTNAG 6 cut(s) 44, 279, 705, 820, 978, 1079
BstDSI CCRYGG 1 cut(s) 883
BstF5I GGATG 2 cut(s) 257, 670
BstFNI CGCG 1 cut(s) 967
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 3 cut(s) 233, 595, 928
BstMAI GTCTC 2 cut(s) 714, 719
BstMBI GATC 3 cut(s) 230, 592, 925
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 3 cut(s) 578, 840, 957
BstSCI CCNGG 4 cut(s) 348, 576, 838, 955
BstUI CGCG 1 cut(s) 967
BstV1I GCAGC 2 cut(s) 123, 886
BstV2I GAAGAC 1 cut(s) 948
BstXI CCANNNNNNTGG 2 cut(s) 884, 936
BsuRI GGCC 3 cut(s) 121, 1036, 1059
BtgI CCRYGG 1 cut(s) 883
BtsCI GGATG 2 cut(s) 257, 670
BtsIMutI CAGTG 2 cut(s) 40, 1013
Cac8I GCNNGC 3 cut(s) 534, 904, 1085
CfoI GCGC 1 cut(s) 969
Cfr13I GGNCC 3 cut(s) 238, 376, 1058
Csp6I GTAC 1 cut(s) 568
CviAII CATG 2 cut(s) 766, 884
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 6 cut(s) 44, 279, 705, 820, 978, 1079
DpnI GATC 3 cut(s) 232, 594, 927
DpnII GATC 3 cut(s) 230, 592, 925
Eam1104I CTCTTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1014
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 2 cut(s) 270, 883
Eco147I AGGCCT 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 719
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 238, 376
Eco53kI GAGCTC 1 cut(s) 790
Eco57I CTGAAG 3 cut(s) 312, 581, 1015
EcoICRI GAGCTC 1 cut(s) 790
EcoO109I RGGNCCY 1 cut(s) 376
EcoRII CCWGG 3 cut(s) 576, 838, 955
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 270, 883
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 2 cut(s) 270, 883
FaeI CATG 2 cut(s) 769, 887
FaiI YATR 5 cut(s) 106, 543, 767, 885, 1133
FaqI GGGAC 1 cut(s) 362
FatI CATG 2 cut(s) 765, 883
Fnu4HI GCNGC 2 cut(s) 112, 900
FokI GGATG 2 cut(s) 264, 677
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 2 cut(s) 112, 900
FspBI CTAG 1 cut(s) 717
GlaI GCGC 1 cut(s) 968
GluI GCNGC 2 cut(s) 112, 900
GsuI CTGGAG 2 cut(s) 115, 822
HaeIII GGCC 3 cut(s) 121, 1036, 1059
HapII CCGG 2 cut(s) 350, 410
HhaI GCGC 1 cut(s) 969
Hin1II CATG 2 cut(s) 769, 887
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HincII GTYRAC 2 cut(s) 82, 178
HindII GTYRAC 2 cut(s) 82, 178
HindIII AAGCTT 3 cut(s) 324, 470, 904
HinfI GANTC 8 cut(s) 86, 184, 538, 707, 775, 818, 962, 1025
HpaII CCGG 2 cut(s) 350, 410
HphI GGTGA 8 cut(s) 170, 175, 304, 659, 686, 866, 971, 994
Hpy166II GTNNAC 4 cut(s) 82, 166, 178, 1010
Hpy188I TCNGA 6 cut(s) 199, 238, 280, 331, 432, 706
Hpy188III TCNNGA 6 cut(s) 94, 379, 440, 688, 781, 1073
Hpy8I GTNNAC 4 cut(s) 82, 166, 178, 1010
HpyAV CCTTC 6 cut(s) 367, 427, 455, 556, 704, 1039
HpyCH4III ACNGT 2 cut(s) 702, 815
HpyCH4IV ACGT 2 cut(s) 288, 808
HpyCH4V TGCA 6 cut(s) 52, 128, 753, 769, 902, 1118
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 6 cut(s) 44, 279, 705, 820, 978, 1079
HpySE526I ACGT 2 cut(s) 288, 808
Hsp92II CATG 2 cut(s) 769, 887
HspAI GCGC 1 cut(s) 967
Kzo9I GATC 3 cut(s) 230, 592, 925
LmnI GCTCC 2 cut(s) 96, 787
Lsp1109I GCAGC 2 cut(s) 123, 886
LweI GCATC 3 cut(s) 115, 396, 504
MaeI CTAG 1 cut(s) 717
MaeII ACGT 2 cut(s) 288, 808
MaeIII GTNAC 5 cut(s) 35, 674, 737, 809, 815
MalI GATC 3 cut(s) 232, 594, 927
MboI GATC 3 cut(s) 230, 592, 925
MboII GAAGA 9 cut(s) 415, 625, 628, 649, 652, 736, 953, 1031, 1034
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 4 cut(s) 683, 888, 1038, 1064
MlyI GAGTC 3 cut(s) 178, 716, 812
MmeI TCCRAC 1 cut(s) 1086
MroXI GAANNNNTTC 2 cut(s) 260, 311
MseI TTAA 8 cut(s) 357, 396, 417, 447, 555, 603, 669, 992
MslI CAYNNNNRTG 1 cut(s) 1053
MspI CCGG 2 cut(s) 350, 410
MspR9I CCNGG 4 cut(s) 350, 578, 840, 957
MvaI CCWGG 3 cut(s) 578, 840, 957
MvnI CGCG 1 cut(s) 967
MwoI GCNNNNNNNGC 1 cut(s) 49
NciI CCSGG 1 cut(s) 350
NcoI CCATGG 1 cut(s) 883
NdeII GATC 3 cut(s) 230, 592, 925
NlaIII CATG 2 cut(s) 769, 887
NlaIV GGNNCC 3 cut(s) 240, 378, 916
NmuCI GTSAC 3 cut(s) 35, 674, 815
PceI AGGCCT 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 260, 311
PfeI GAWTC 5 cut(s) 86, 538, 775, 962, 1025
PflFI GACNNNGTC 1 cut(s) 821
PkrI GCNGC 2 cut(s) 113, 901
PleI GAGTC 3 cut(s) 178, 715, 812
PpsI GAGTC 3 cut(s) 178, 715, 812
PpuMI RGGWCCY 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 792
Psp5II RGGWCCY 1 cut(s) 376
Psp6I CCWGG 3 cut(s) 576, 838, 955
PspGI CCWGG 3 cut(s) 576, 838, 955
PspN4I GGNNCC 3 cut(s) 240, 378, 916
PspPI GGNCC 3 cut(s) 238, 376, 1058
PspPPI RGGWCCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 821
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 8 cut(s) 357, 396, 417, 447, 555, 603, 669, 992
SatI GCNGC 2 cut(s) 112, 900
Sau3AI GATC 3 cut(s) 230, 592, 925
Sau96I GGNCC 3 cut(s) 238, 376, 1058
SchI GAGTC 3 cut(s) 178, 716, 812
ScrFI CCNGG 4 cut(s) 350, 578, 840, 957
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 3 cut(s) 115, 396, 504
SinI GGWCC 2 cut(s) 238, 376
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 4 cut(s) 683, 888, 1038, 1064
SseBI AGGCCT 1 cut(s) 121
SsiI CCGC 2 cut(s) 133, 156
SspI AATATT 1 cut(s) 553
SspMI CTAG 1 cut(s) 717
SstI GAGCTC 1 cut(s) 792
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 4 cut(s) 348, 576, 838, 955
StyI CCWWGG 2 cut(s) 270, 883
TaaI ACNGT 2 cut(s) 702, 815
TaiI ACGT 2 cut(s) 291, 811
TaqI TCGA 1 cut(s) 141
TaqII GACCGA 1 cut(s) 67
TasI AATT 4 cut(s) 683, 888, 1038, 1064
TfiI GAWTC 5 cut(s) 86, 538, 775, 962, 1025
Tru1I TTAA 8 cut(s) 357, 396, 417, 447, 555, 603, 669, 992
Tru9I TTAA 8 cut(s) 357, 396, 417, 447, 555, 603, 669, 992
TscAI CASTG 2 cut(s) 40, 1020
TseFI GTSAC 3 cut(s) 35, 674, 815
TseI GCWGC 2 cut(s) 111, 899
Tsp45I GTSAC 3 cut(s) 35, 674, 815
TspDTI ATGAA 3 cut(s) 474, 530, 1034
TspGWI ACGGA 2 cut(s) 815, 1058
TspRI CASTG 2 cut(s) 40, 1020
Tth111I GACNNNGTC 1 cut(s) 821
VpaK11BI GGWCC 2 cut(s) 238, 376
XapI RAATTY 3 cut(s) 683, 888, 1064
XmnI GAANNNNTTC 2 cut(s) 260, 311
XspI CTAG 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.