RchiOBHm_Chr7g0194091

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011698
Physical Location & Seq
Forward (+)
12295419 .. 12298453
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17358

Sequence Viewer

Length: 1221 bp
ATGGAGAACAACAACAACCAGTCCTTCTGGCAATTCAGCGACCAGCTTCGTGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGCTCATACGGCAGCAAGAGGCCTGATGCGAGGAGGAACTTCGACATCAAGGTCGGCGGTGAGGTGAATTCTTTGATTAATTTGACCCCAAAAGGGTCTGATTTCAATGGCTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTCAGCGACAGGTTTAACAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTCAATTCTTTCAACCCAAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTTAATGGGTTTAACCAGAAAGTCCCGGACTTCAATGGGTTTAATCAGAAGGTCCCGGACTTCAATGGATTCAACCAGAAGGTTCCGGATTTTAACGGGTTTAATGAAGGGTGGAAACTCGGGGCTTCTGCTAATGGTGGCATCAATGGAGGCTTCAACAAGGGAATTTACTCCAAGCCTGCAATTCACAACAATAACATTAACCTGAACCTGAAGCCGTACAAGAACCAGGGTGAGGAGTTTGATCTTGGAGTTAAAGCTGGGAAGAAGAACAACAATAGCAACAAGAAGAAAAATGGTGATGACAATAACAAGGACGTCAAGGGTGACAAGAAATTCAAGACACTTCCACCCTCTGAGTCTCTCCCCAGAAATGAAACCATTGGTGGTTACATCTTCGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGCAACTTTTTGGTTTGCCTCCACGTTACCGTGACTCAGTTCGGGCCATTACTCCAGGGTTGCCCCTTTTCCTTTACAACTACTCCACCCACCAACTCCATGGAATTTTTGAGGCTGCAAGCTTTGGAGGAACCAACTTTGATCCATCTGCTTGGGAAGACAAGAAATGCCCTGGTGAATCGCGCTTCCCTGCTCAGGTGAAAGTTCTAACTAGGAAAGTCTGTGAACCACTGGAAGAGGATTCCTTCAGGCCAATTCTTCATCACTACGATGGCCCTAAATTCCGTCTTGAACTCAGCGTGCCAGAGGCCCTCTCTCTGTTGGATATATTTGCAGACCAAGATGATATATTTGTAACTGAAACCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

406

Amino Acids

45.65

Weight (kDa)

6.97

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 267 - 394 1.1e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 735
AccII CGCG 1 cut(s) 1033
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 1 cut(s) 159
AclWI GGATC 1 cut(s) 986
AcsI RAATTY 6 cut(s) 169, 293, 579, 749, 954, 1130
AcuI CTGAAG 4 cut(s) 303, 363, 647, 1081
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 5 cut(s) 195, 259, 260, 649, 1045
AjnI CCWGG 3 cut(s) 642, 904, 1021
AluBI AGCT 7 cut(s) 46, 105, 273, 317, 377, 674, 972
AluI AGCT 7 cut(s) 46, 105, 273, 317, 377, 674, 972
Alw26I GTCTC 1 cut(s) 780
AlwI GGATC 1 cut(s) 986
Ama87I CYCGRG 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 5 cut(s) 122, 894, 1100, 1123, 1158
ApeKI GCWGC 3 cut(s) 102, 114, 965
ApoI RAATTY 6 cut(s) 169, 293, 579, 749, 954, 1130
AseI ATTAAT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 1035
AspS9I GGNCC 5 cut(s) 246, 466, 894, 1124, 1159
AsuC2I CCSGG 2 cut(s) 440, 470
AsuHPI GGTGA 7 cut(s) 173, 178, 659, 725, 752, 1037, 1060
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 246, 466
BbsI GAAGAC 1 cut(s) 1014
BbvI GCAGC 3 cut(s) 114, 126, 952
BccI CCATC 2 cut(s) 1003, 1115
BceAI ACGGC 2 cut(s) 127, 616
BciT130I CCWGG 3 cut(s) 644, 906, 1023
BcnI CCSGG 2 cut(s) 440, 470
BcoDI GTCTC 1 cut(s) 780
BfaI CTAG 1 cut(s) 1062
BisI GCNGC 3 cut(s) 103, 115, 966
BlsI GCNGC 3 cut(s) 104, 116, 967
Bme1390I CCNGG 5 cut(s) 440, 470, 644, 906, 1023
Bme18I GGWCC 2 cut(s) 246, 466
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 5 cut(s) 246, 466, 894, 1124, 1159
BmiI GGNNCC 4 cut(s) 247, 468, 498, 982
BmrFI CCNGG 5 cut(s) 440, 470, 644, 906, 1023
BmsI GCATC 2 cut(s) 118, 564
BpiI GAAGAC 1 cut(s) 1014
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 2 cut(s) 118, 888
Bpu10I CCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 2 cut(s) 440, 470
BsaHI GRCGYC 1 cut(s) 732
BsaJI CCNNGG 5 cut(s) 282, 643, 905, 949, 1021
BsaWI WCCGGW 1 cut(s) 499
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 259, 260, 649, 1045
Bse1I ACTGG 2 cut(s) 19, 1086
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 3 cut(s) 644, 906, 1023
BseDI CCNNGG 5 cut(s) 282, 643, 905, 949, 1021
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 5 cut(s) 195, 259, 260, 649, 1045
BseMII CTCAG 4 cut(s) 762, 900, 1058, 1159
BseNI ACTGG 2 cut(s) 19, 1086
BseRI GAGGAG 2 cut(s) 148, 665
BseXI GCAGC 3 cut(s) 114, 126, 952
BseYI CCCAGC 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 1033
BshFI GGCC 5 cut(s) 124, 896, 1102, 1125, 1160
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 3 cut(s) 440, 470, 500
BslFI GGGAC 2 cut(s) 422, 452
BslI CCNNNNNNNGG 5 cut(s) 195, 259, 260, 649, 1045
BsmAI GTCTC 1 cut(s) 780
BsmFI GGGAC 2 cut(s) 422, 452
BsnI GGCC 5 cut(s) 124, 896, 1102, 1125, 1160
BsoBI CYCGRG 1 cut(s) 533
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 93, 658, 991
Bsp19I CCATGG 1 cut(s) 949
BspACI CCGC 1 cut(s) 159
BspANI GGCC 5 cut(s) 124, 896, 1102, 1125, 1160
BspCNI CTCAG 4 cut(s) 763, 899, 1057, 1158
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 247, 468, 498, 982
BspPI GGATC 1 cut(s) 986
BsrI ACTGG 2 cut(s) 19, 1086
BssECI CCNNGG 5 cut(s) 282, 643, 905, 949, 1021
BssMI GATC 3 cut(s) 93, 658, 991
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 2 cut(s) 282, 949
Bst2UI CCWGG 3 cut(s) 644, 906, 1023
Bst4CI ACNGT 1 cut(s) 881
Bst6I CTCTTC 1 cut(s) 1080
BstACI GRCGYC 1 cut(s) 732
BstC8I GCNNGC 3 cut(s) 594, 970, 1151
BstDEI CTNAG 4 cut(s) 771, 886, 1044, 1145
BstDSI CCRYGG 1 cut(s) 949
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 1 cut(s) 1033
BstHHI GCGC 1 cut(s) 1035
BstKTI GATC 3 cut(s) 96, 661, 994
BstMAI GTCTC 1 cut(s) 780
BstMBI GATC 3 cut(s) 93, 658, 991
BstMWI GCNNNNNNNGC 2 cut(s) 111, 270
BstNI CCWGG 3 cut(s) 644, 906, 1023
BstSCI CCNGG 5 cut(s) 438, 468, 642, 904, 1021
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 3 cut(s) 114, 126, 952
BstV2I GAAGAC 1 cut(s) 1014
BstXI CCANNNNNNTGG 2 cut(s) 950, 1002
BsuRI GGCC 5 cut(s) 124, 896, 1102, 1125, 1160
BtgI CCRYGG 1 cut(s) 949
BtsCI GGATG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 1079
Cac8I GCNNGC 3 cut(s) 594, 970, 1151
CfoI GCGC 1 cut(s) 1035
Cfr13I GGNCC 5 cut(s) 246, 466, 894, 1124, 1159
Csp6I GTAC 1 cut(s) 634
CviAII CATG 2 cut(s) 832, 950
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 4 cut(s) 771, 886, 1044, 1145
DpnI GATC 3 cut(s) 95, 660, 993
DpnII GATC 3 cut(s) 93, 658, 991
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 1080
EarI CTCTTC 1 cut(s) 1080
Eco130I CCWWGG 2 cut(s) 282, 949
Eco147I AGGCCT 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 246, 466
Eco57I CTGAAG 4 cut(s) 303, 363, 647, 1081
Eco88I CYCGRG 1 cut(s) 533
EcoO109I RGGNCCY 2 cut(s) 466, 1159
EcoRI GAATTC 2 cut(s) 169, 293
EcoRII CCWGG 3 cut(s) 642, 904, 1021
EcoT14I CCWWGG 2 cut(s) 282, 949
ErhI CCWWGG 2 cut(s) 282, 949
FaeI CATG 2 cut(s) 835, 953
FaiI YATR 5 cut(s) 109, 833, 951, 1178, 1199
FaqI GGGAC 2 cut(s) 422, 452
FatI CATG 2 cut(s) 831, 949
Fnu4HI GCNGC 3 cut(s) 103, 115, 966
FokI GGATG 1 cut(s) 46
Fsp4HI GCNGC 3 cut(s) 103, 115, 966
FspBI CTAG 1 cut(s) 1062
GlaI GCGC 1 cut(s) 1034
GluI GCNGC 3 cut(s) 103, 115, 966
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 2 cut(s) 118, 888
HaeIII GGCC 5 cut(s) 124, 896, 1102, 1125, 1160
HapII CCGG 3 cut(s) 440, 470, 500
HhaI GCGC 1 cut(s) 1035
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 2 cut(s) 835, 953
Hin6I GCGC 1 cut(s) 1033
HinP1I GCGC 1 cut(s) 1033
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 3 cut(s) 315, 375, 970
HinfI GANTC 7 cut(s) 89, 483, 773, 841, 884, 1028, 1091
HpaII CCGG 3 cut(s) 440, 470, 500
HphI GGTGA 7 cut(s) 173, 178, 659, 725, 752, 1037, 1060
Hpy166II GTNNAC 2 cut(s) 85, 1076
Hpy188I TCNGA 9 cut(s) 202, 250, 292, 322, 352, 382, 412, 462, 772
Hpy188III TCNNGA 6 cut(s) 55, 97, 500, 754, 847, 1139
Hpy8I GTNNAC 2 cut(s) 85, 1076
HpyAV CCTTC 6 cut(s) 34, 215, 457, 487, 515, 1105
HpyCH4III ACNGT 1 cut(s) 881
HpyCH4IV ACGT 2 cut(s) 732, 874
HpyCH4V TGCA 5 cut(s) 596, 819, 835, 968, 1184
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 270
HpyF3I CTNAG 4 cut(s) 771, 886, 1044, 1145
HpySE526I ACGT 2 cut(s) 732, 874
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 2 cut(s) 835, 953
HspAI GCGC 1 cut(s) 1033
Kpn2I TCCGGA 1 cut(s) 499
Kzo9I GATC 3 cut(s) 93, 658, 991
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 3 cut(s) 114, 126, 952
LweI GCATC 2 cut(s) 118, 564
MaeI CTAG 1 cut(s) 1062
MaeII ACGT 2 cut(s) 732, 874
MaeIII GTNAC 5 cut(s) 740, 803, 875, 881, 1204
MalI GATC 3 cut(s) 95, 660, 993
MboI GATC 3 cut(s) 93, 658, 991
MboII GAAGA 7 cut(s) 691, 694, 715, 802, 1019, 1097, 1100
MlyI GAGTC 3 cut(s) 83, 782, 878
MmeI TCCRAC 2 cut(s) 273, 1152
MroI TCCGGA 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 9 cut(s) 180, 309, 417, 426, 456, 507, 516, 615, 669
MslI CAYNNNNRTG 1 cut(s) 1119
MspI CCGG 3 cut(s) 440, 470, 500
MspR9I CCNGG 5 cut(s) 440, 470, 644, 906, 1023
MvaI CCWGG 3 cut(s) 644, 906, 1023
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 2 cut(s) 111, 270
NciI CCSGG 2 cut(s) 440, 470
NcoI CCATGG 1 cut(s) 949
NdeII GATC 3 cut(s) 93, 658, 991
NlaIII CATG 2 cut(s) 835, 953
NlaIV GGNNCC 4 cut(s) 247, 468, 498, 982
NmuCI GTSAC 2 cut(s) 740, 881
PceI AGGCCT 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 4 cut(s) 483, 841, 1028, 1091
PfoI TCCNGGA 2 cut(s) 438, 468
PkrI GCNGC 3 cut(s) 104, 116, 967
PleI GAGTC 3 cut(s) 83, 781, 878
PpsI GAGTC 3 cut(s) 83, 781, 878
PpuMI RGGWCCY 1 cut(s) 466
PshBI ATTAAT 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 3 cut(s) 642, 904, 1021
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 3 cut(s) 642, 904, 1021
PspN4I GGNNCC 4 cut(s) 247, 468, 498, 982
PspPI GGNCC 5 cut(s) 246, 466, 894, 1124, 1159
PspPPI RGGWCCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 1119
SaqAI TTAA 9 cut(s) 180, 309, 417, 426, 456, 507, 516, 615, 669
SatI GCNGC 3 cut(s) 103, 115, 966
Sau3AI GATC 3 cut(s) 93, 658, 991
Sau96I GGNCC 5 cut(s) 246, 466, 894, 1124, 1159
SchI GAGTC 3 cut(s) 83, 782, 878
ScrFI CCNGG 5 cut(s) 440, 470, 644, 906, 1023
SfaNI GCATC 2 cut(s) 118, 564
SinI GGWCC 2 cut(s) 246, 466
SmiMI CAYNNNNRTG 1 cut(s) 1119
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SseBI AGGCCT 1 cut(s) 124
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 1062
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 5 cut(s) 438, 468, 642, 904, 1021
StyI CCWWGG 2 cut(s) 282, 949
TaaI ACNGT 1 cut(s) 881
TaiI ACGT 2 cut(s) 735, 877
TaqI TCGA 2 cut(s) 92, 144
TfiI GAWTC 4 cut(s) 483, 841, 1028, 1091
Tru1I TTAA 9 cut(s) 180, 309, 417, 426, 456, 507, 516, 615, 669
Tru9I TTAA 9 cut(s) 180, 309, 417, 426, 456, 507, 516, 615, 669
TscAI CASTG 1 cut(s) 1086
TseFI GTSAC 2 cut(s) 740, 881
TseI GCWGC 3 cut(s) 102, 114, 965
Tsp45I GTSAC 2 cut(s) 740, 881
TspDTI ATGAA 4 cut(s) 234, 534, 804, 1100
TspGWI ACGGA 2 cut(s) 70, 1124
TspRI CASTG 1 cut(s) 1086
VpaK11BI GGWCC 2 cut(s) 246, 466
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 6 cut(s) 169, 293, 579, 749, 954, 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 947
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 1 cut(s) 1062
ZraI GACGTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.