Rroxscaffold_3G00261560

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011698
Physical Location & Seq
Reverse (-)
55809130 .. 55811390
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00261560.1

Sequence Viewer

Length: 1263 bp
ATGGAGAACAACAACAACAATCAATCCTTCTGGCAATTCAGCGACCAGCTTCGTGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGCTCCTACGGCAGCAAGAGGCCTGATGCGAGGAGGAACTTCGACATCAAGGTCGGCGGTGAGGTGAATTCTTTGATTAATTTGACCCCAAAAGGGTCTGATTTCAATGGGTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTGAACGGCTTCAGCGACAGGTTTAACAAAGCTTCAGACTTCAATTCTTTCAACCCGAAAGCTTCAGACTTCAATTCTTTCAACCCGAAAGTTTCAGACTTCAATTCTTTCAACCCAAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTTAATGGGTTTAACCAGAAGGTCCCGGACTTCAATGGGTTTAATCAGAAGGTCCCGGACTTCAATGGGTTCAACCAGAAGGTTCCGGATTTTAACGGGTTTAACGAAGGGTGGAAACTCGGGGCTTCTGCTAACGGTGGCATCAATGGAGGCTTCAACAAGGGGATTTACTCCAAGCCTGCAATTCACAACAATAACATTAACCTGAACCTGAAGTCCTACAAGAACCAGGGTGAGGAGTTTGATCTTGGAGTTAAAGCTGGGAAGAAGAACAACAATAGCAACAAGAAGAAAAATGGTGATGACAATAACAAGGACGTCAAGGGTGACAAGAAATTCAAGACACTTCCACCGTCTGAGTCTCTCCCCAGAAATGAAACCATTGGTGGTTACATCTTCGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGCAGCTTTTTGGTTTGCCTCCACGTTACCGTGACTCAGTTCGGGCCATTACTCCAGGGTTGCCCCTTTTCCTTTACAACTACTCCACTCACCAACTCCATGGAATTTTTGAGGCTGCAAGCTTTGGAGGAACCAACTTTGATCCATCTGCTTGGGAAGACAAGAAATGCCCTGGTGAATCGCGCTTCCCTGCTCAGGTGAAAGTTCTAACTAGGAAAGTCTGTGAACCACTGGAAGAGGATTCCTTCAGGCCAATTCTTCATCACTACGATGGCCCTAAATTCCGTCTTGAACTCAGCGTGCCAGAGGCCCTCTCTCTGTTGGATATATTTGCAGACCAGGATGATATATTTGTAACTGAAACCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

420

Amino Acids

47.14

Weight (kDa)

6.97

Isoelectric Point (pI)

25.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 281 - 408 1.1e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AatII GACGTC 1 cut(s) 777
AccII CGCG 1 cut(s) 1075
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 1 cut(s) 162
AclWI GGATC 1 cut(s) 1028
AcsI RAATTY 4 cut(s) 172, 791, 996, 1172
AcuI CTGAAG 6 cut(s) 292, 315, 345, 405, 689, 1123
AcyI GRCGYC 1 cut(s) 774
AfiI CCNNNNNNNGG 5 cut(s) 198, 262, 263, 691, 1087
AjnI CCWGG 4 cut(s) 684, 946, 1063, 1230
AloI GAACNNNNNNTCC 2 cut(s) 656, 688
AluBI AGCT 9 cut(s) 49, 108, 276, 329, 359, 419, 716, 898, 1014
AluI AGCT 9 cut(s) 49, 108, 276, 329, 359, 419, 716, 898, 1014
Alw26I GTCTC 1 cut(s) 822
AlwI GGATC 1 cut(s) 1028
Ama87I CYCGRG 1 cut(s) 575
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 5 cut(s) 125, 936, 1142, 1165, 1200
ApeKI GCWGC 4 cut(s) 105, 117, 895, 1007
ApoI RAATTY 4 cut(s) 172, 791, 996, 1172
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 2 cut(s) 275, 305
AspLEI GCGC 1 cut(s) 1077
AspS9I GGNCC 6 cut(s) 249, 478, 508, 936, 1166, 1201
AsuC2I CCSGG 2 cut(s) 482, 512
AsuHPI GGTGA 8 cut(s) 176, 181, 701, 767, 794, 974, 1079, 1102
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 3 cut(s) 249, 478, 508
BbsI GAAGAC 1 cut(s) 1056
BbvI GCAGC 4 cut(s) 117, 129, 907, 994
BccI CCATC 2 cut(s) 1045, 1157
BceAI ACGGC 2 cut(s) 130, 319
BciT130I CCWGG 4 cut(s) 686, 948, 1065, 1232
BcnI CCSGG 2 cut(s) 482, 512
BcoDI GTCTC 1 cut(s) 822
BfaI CTAG 1 cut(s) 1104
BisI GCNGC 4 cut(s) 106, 118, 896, 1008
BlsI GCNGC 4 cut(s) 107, 119, 897, 1009
Bme1390I CCNGG 6 cut(s) 482, 512, 686, 948, 1065, 1232
Bme18I GGWCC 3 cut(s) 249, 478, 508
BmeT110I CYCGRG 1 cut(s) 575
BmgT120I GGNCC 6 cut(s) 249, 478, 508, 936, 1166, 1201
BmiI GGNNCC 5 cut(s) 250, 480, 510, 540, 1024
BmrFI CCNGG 6 cut(s) 482, 512, 686, 948, 1065, 1232
BmsI GCATC 2 cut(s) 121, 606
BpiI GAAGAC 1 cut(s) 1056
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BpmI CTGGAG 2 cut(s) 121, 930
Bpu10I CCTNAGC 1 cut(s) 1086
BpuEI CTTGAG 1 cut(s) 872
BpuMI CCSGG 2 cut(s) 482, 512
BsaHI GRCGYC 1 cut(s) 774
BsaJI CCNNGG 5 cut(s) 285, 685, 947, 991, 1063
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 262, 263, 691, 1087
Bse1I ACTGG 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 4 cut(s) 686, 948, 1065, 1232
BseDI CCNNGG 5 cut(s) 285, 685, 947, 991, 1063
BseGI GGATG 2 cut(s) 62, 1240
BseLI CCNNNNNNNGG 5 cut(s) 198, 262, 263, 691, 1087
BseMII CTCAG 4 cut(s) 804, 942, 1100, 1201
BseNI ACTGG 1 cut(s) 1128
BseRI GAGGAG 2 cut(s) 151, 707
BseXI GCAGC 4 cut(s) 117, 129, 907, 994
BseYI CCCAGC 1 cut(s) 716
Bsh1236I CGCG 1 cut(s) 1075
BshFI GGCC 5 cut(s) 127, 938, 1144, 1167, 1202
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 3 cut(s) 482, 512, 542
BslFI GGGAC 2 cut(s) 464, 494
BslI CCNNNNNNNGG 5 cut(s) 198, 262, 263, 691, 1087
BsmAI GTCTC 1 cut(s) 822
BsmFI GGGAC 2 cut(s) 464, 494
BsnI GGCC 5 cut(s) 127, 938, 1144, 1167, 1202
BsoBI CYCGRG 1 cut(s) 575
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 3 cut(s) 96, 700, 1033
Bsp19I CCATGG 1 cut(s) 991
BspACI CCGC 1 cut(s) 162
BspANI GGCC 5 cut(s) 127, 938, 1144, 1167, 1202
BspCNI CTCAG 4 cut(s) 805, 941, 1099, 1200
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 1075
BspLI GGNNCC 5 cut(s) 250, 480, 510, 540, 1024
BspPI GGATC 1 cut(s) 1028
BsrI ACTGG 1 cut(s) 1128
BssECI CCNNGG 5 cut(s) 285, 685, 947, 991, 1063
BssMI GATC 3 cut(s) 96, 700, 1033
BssNI GRCGYC 1 cut(s) 774
BssT1I CCWWGG 2 cut(s) 285, 991
Bst2UI CCWGG 4 cut(s) 686, 948, 1065, 1232
Bst4CI ACNGT 3 cut(s) 593, 810, 923
Bst6I CTCTTC 1 cut(s) 1122
BstACI GRCGYC 1 cut(s) 774
BstC8I GCNNGC 3 cut(s) 636, 1012, 1193
BstDEI CTNAG 4 cut(s) 813, 928, 1086, 1187
BstDSI CCRYGG 1 cut(s) 991
BstF5I GGATG 2 cut(s) 62, 1240
BstFNI CGCG 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 1077
BstKTI GATC 3 cut(s) 99, 703, 1036
BstMAI GTCTC 1 cut(s) 822
BstMBI GATC 3 cut(s) 96, 700, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 114, 273
BstNI CCWGG 4 cut(s) 686, 948, 1065, 1232
BstSCI CCNGG 6 cut(s) 480, 510, 684, 946, 1063, 1230
BstUI CGCG 1 cut(s) 1075
BstV1I GCAGC 4 cut(s) 117, 129, 907, 994
BstV2I GAAGAC 1 cut(s) 1056
BstXI CCANNNNNNTGG 2 cut(s) 992, 1044
BsuRI GGCC 5 cut(s) 127, 938, 1144, 1167, 1202
BtgI CCRYGG 1 cut(s) 991
BtsCI GGATG 2 cut(s) 62, 1240
BtsIMutI CAGTG 1 cut(s) 1121
Cac8I GCNNGC 3 cut(s) 636, 1012, 1193
CfoI GCGC 1 cut(s) 1077
Cfr13I GGNCC 6 cut(s) 249, 478, 508, 936, 1166, 1201
CviAII CATG 2 cut(s) 874, 992
DdeI CTNAG 4 cut(s) 813, 928, 1086, 1187
DpnI GATC 3 cut(s) 98, 702, 1035
DpnII GATC 3 cut(s) 96, 700, 1033
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Eco130I CCWWGG 2 cut(s) 285, 991
Eco147I AGGCCT 1 cut(s) 127
Eco47I GGWCC 3 cut(s) 249, 478, 508
Eco57I CTGAAG 6 cut(s) 292, 315, 345, 405, 689, 1123
Eco88I CYCGRG 1 cut(s) 575
EcoO109I RGGNCCY 3 cut(s) 478, 508, 1201
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 4 cut(s) 684, 946, 1063, 1230
EcoT14I CCWWGG 2 cut(s) 285, 991
ErhI CCWWGG 2 cut(s) 285, 991
FaeI CATG 2 cut(s) 877, 995
FaiI YATR 4 cut(s) 875, 993, 1220, 1241
FaqI GGGAC 2 cut(s) 464, 494
FatI CATG 2 cut(s) 873, 991
Fnu4HI GCNGC 4 cut(s) 106, 118, 896, 1008
FokI GGATG 2 cut(s) 49, 1247
Fsp4HI GCNGC 4 cut(s) 106, 118, 896, 1008
FspBI CTAG 1 cut(s) 1104
GlaI GCGC 1 cut(s) 1076
GluI GCNGC 4 cut(s) 106, 118, 896, 1008
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 2 cut(s) 121, 930
HaeIII GGCC 5 cut(s) 127, 938, 1144, 1167, 1202
HapII CCGG 3 cut(s) 482, 512, 542
HhaI GCGC 1 cut(s) 1077
Hin1I GRCGYC 1 cut(s) 774
Hin1II CATG 2 cut(s) 877, 995
Hin6I GCGC 1 cut(s) 1075
HinP1I GCGC 1 cut(s) 1075
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 4 cut(s) 327, 357, 417, 1012
HinfI GANTC 6 cut(s) 92, 815, 883, 926, 1070, 1133
HpaII CCGG 3 cut(s) 482, 512, 542
HphI GGTGA 8 cut(s) 176, 181, 701, 767, 794, 974, 1079, 1102
Hpy166II GTNNAC 2 cut(s) 88, 1118
Hpy188III TCNNGA 6 cut(s) 58, 100, 542, 796, 889, 1181
Hpy8I GTNNAC 2 cut(s) 88, 1118
HpyAV CCTTC 7 cut(s) 37, 218, 469, 499, 529, 557, 1147
HpyCH4III ACNGT 3 cut(s) 593, 810, 923
HpyCH4IV ACGT 2 cut(s) 774, 916
HpyCH4V TGCA 5 cut(s) 638, 861, 877, 1010, 1226
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 273
HpyF3I CTNAG 4 cut(s) 813, 928, 1086, 1187
HpySE526I ACGT 2 cut(s) 774, 916
Hsp92I GRCGYC 1 cut(s) 774
Hsp92II CATG 2 cut(s) 877, 995
HspAI GCGC 1 cut(s) 1075
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 96, 700, 1033
LmnI GCTCC 2 cut(s) 102, 113
Lsp1109I GCAGC 4 cut(s) 117, 129, 907, 994
LweI GCATC 2 cut(s) 121, 606
MaeI CTAG 1 cut(s) 1104
MaeII ACGT 2 cut(s) 774, 916
MaeIII GTNAC 5 cut(s) 782, 845, 917, 923, 1246
MalI GATC 3 cut(s) 98, 702, 1035
MboI GATC 3 cut(s) 96, 700, 1033
MboII GAAGA 7 cut(s) 733, 736, 757, 844, 1061, 1139, 1142
MlyI GAGTC 3 cut(s) 86, 824, 920
MmeI TCCRAC 2 cut(s) 276, 1194
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 2 cut(s) 275, 305
MseI TTAA 9 cut(s) 183, 321, 459, 468, 498, 549, 558, 657, 711
MslI CAYNNNNRTG 1 cut(s) 1161
MspI CCGG 3 cut(s) 482, 512, 542
MspR9I CCNGG 6 cut(s) 482, 512, 686, 948, 1065, 1232
MvaI CCWGG 4 cut(s) 686, 948, 1065, 1232
MvnI CGCG 1 cut(s) 1075
MwoI GCNNNNNNNGC 2 cut(s) 114, 273
NciI CCSGG 2 cut(s) 482, 512
NcoI CCATGG 1 cut(s) 991
NdeII GATC 3 cut(s) 96, 700, 1033
NlaIII CATG 2 cut(s) 877, 995
NlaIV GGNNCC 5 cut(s) 250, 480, 510, 540, 1024
NmuCI GTSAC 2 cut(s) 782, 923
PceI AGGCCT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 558
PdmI GAANNNNTTC 2 cut(s) 275, 305
PfeI GAWTC 3 cut(s) 883, 1070, 1133
PfoI TCCNGGA 2 cut(s) 480, 510
PkrI GCNGC 4 cut(s) 107, 119, 897, 1009
PleI GAGTC 3 cut(s) 86, 823, 920
PpsI GAGTC 3 cut(s) 86, 823, 920
PpuMI RGGWCCY 2 cut(s) 478, 508
PshBI ATTAAT 1 cut(s) 183
Psp5II RGGWCCY 2 cut(s) 478, 508
Psp6I CCWGG 4 cut(s) 684, 946, 1063, 1230
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 4 cut(s) 684, 946, 1063, 1230
PspN4I GGNNCC 5 cut(s) 250, 480, 510, 540, 1024
PspPI GGNCC 6 cut(s) 249, 478, 508, 936, 1166, 1201
PspPPI RGGWCCY 2 cut(s) 478, 508
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 9 cut(s) 183, 321, 459, 468, 498, 549, 558, 657, 711
SatI GCNGC 4 cut(s) 106, 118, 896, 1008
Sau3AI GATC 3 cut(s) 96, 700, 1033
Sau96I GGNCC 6 cut(s) 249, 478, 508, 936, 1166, 1201
SchI GAGTC 3 cut(s) 86, 824, 920
ScrFI CCNGG 6 cut(s) 482, 512, 686, 948, 1065, 1232
SfaNI GCATC 2 cut(s) 121, 606
SinI GGWCC 3 cut(s) 249, 478, 508
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 1 cut(s) 162
SspMI CTAG 1 cut(s) 1104
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 6 cut(s) 480, 510, 684, 946, 1063, 1230
StyI CCWWGG 2 cut(s) 285, 991
TaaI ACNGT 3 cut(s) 593, 810, 923
TaiI ACGT 2 cut(s) 777, 919
TaqI TCGA 2 cut(s) 95, 147
TfiI GAWTC 3 cut(s) 883, 1070, 1133
Tru1I TTAA 9 cut(s) 183, 321, 459, 468, 498, 549, 558, 657, 711
Tru9I TTAA 9 cut(s) 183, 321, 459, 468, 498, 549, 558, 657, 711
TscAI CASTG 1 cut(s) 1128
TseFI GTSAC 2 cut(s) 782, 923
TseI GCWGC 4 cut(s) 105, 117, 895, 1007
Tsp45I GTSAC 2 cut(s) 782, 923
TspDTI ATGAA 3 cut(s) 237, 846, 1142
TspGWI ACGGA 2 cut(s) 73, 1166
TspRI CASTG 1 cut(s) 1128
VpaK11BI GGWCC 3 cut(s) 249, 478, 508
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 4 cut(s) 172, 791, 996, 1172
XmnI GAANNNNTTC 2 cut(s) 275, 305
XspI CTAG 1 cut(s) 1104
ZraI GACGTC 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.