MD14G1131300.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011698
Physical Location & Seq
Reverse (-)
20862031 .. 20864614
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1131300.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGAGAACAACCAACAGTCGTTTTGGCAGTTCAGCGATCAGCTTCGAGTCCAAGGCTCCAATTTCGCAAATCTGAACATCAACGATTCGATTTGGAGCAATTCGTACAGCAAGAGGCCTGATGAGAGGAGGAATTTCGATATAAAGGTCGGCGGAGAAGTGAGTCCGATAGTTAATTTGACCCCGAAAGGGTCCGATTTCAACGGGTTCAACGACTCGTGGGTCGACAATCTGAAGCCGAAAGCGGCGTTTTCCGACGCCAACGGCAGTTCCAATGACGGATGGAATAGCTTCAAGCCGAAAGCTTCGGAGTTGAACAACGGATTCAACGACAACAGATGGAATAGCTTCAACCCAAAGGCTTCGGACTTCAACGACAACAGATGGAACAGCTTCAACCCAAAAGCTTCGGACTTCAACAACGCTTTCAACGACGGGTGGAAACTCGGCGGCGCTGCCGGGTCGCAGAATAGCGCCGTTGGTCTCAATGGCGGTTTCAACAAGGGGATTTACTCCAAGCCTGCAATTCACAACAACAACAACAACTTTATGAATTTGAAGCCGTACAAGAACAAGGCCCACGAGGAAGATCAGATTCATGGCGGGAAAACTGGGAAGAAGAACAACAGCAACAGCAATCCCGGTAAGAAGAAATCCGGCGGCGGCGGCGATGACAATAACAAGGATGGCAAGAGTGGCGTGGATAAGAGGTTTAAGACGCTTCCGCCGGCCGAGTCTCTGCCTAGAAATGAGACCATTGGTGGGTACATCTTTGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGGAGCTTTTTGGATTGCCTCCACGATATCGTGATTCAGTAAGGGCGATAACTCCGGGGTTGCCCCTGTTCCTCTACAACTACTCCACCCACCAACTCCATGGAATTTTTGAGGCTGCGAGTTTTGGAGGGACCAACTTTGATCCATCGGCCTGGGAGGACAAGAAGTGTCCCGGCGAGTCACGCTTTCCAGCTCAGGTTCGAGTTTTTACTCGGAAAGTTTGTGAGCCACTCGAAGAGGACTCGTTCAGGCCAATTCTTCACCACTACGACGGCCCTAAATTCCGACTTGAACTTAGCGTACCCGAGGCACTGTCTCTGTTGGATATATTTGCAGACCAAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

387

Amino Acids

43.11

Weight (kDa)

8.24

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 254 - 381 1.8e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 225
AciI CCGC 9 cut(s) 153, 245, 450, 492, 603, 660, 663, 666, 725
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 4 cut(s) 133, 553, 915, 1091
AcuI CTGAAG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 4 cut(s) 107, 566, 767, 1113
AfiI CCNNNNNNNGG 2 cut(s) 189, 762
AjnI CCWGG 1 cut(s) 962
AluBI AGCT 8 cut(s) 43, 291, 305, 348, 393, 407, 817, 1004
AluI AGCT 8 cut(s) 43, 291, 305, 348, 393, 407, 817, 1004
Alw26I GTCTC 4 cut(s) 488, 741, 746, 1131
AlwI GGATC 1 cut(s) 947
Ama87I CYCGRG 1 cut(s) 1115
AoxI GGCC 6 cut(s) 116, 576, 729, 960, 1061, 1084
ApeKI GCWGC 2 cut(s) 455, 926
ApoI RAATTY 4 cut(s) 133, 553, 915, 1091
Asp700I GAANNNNTTC 3 cut(s) 290, 347, 392
AspLEI GCGC 2 cut(s) 455, 476
AspS9I GGNCC 4 cut(s) 192, 577, 942, 1085
AsuC2I CCSGG 4 cut(s) 460, 642, 867, 984
AsuHPI GGTGA 1 cut(s) 1064
AvaI CYCGRG 1 cut(s) 1115
AvaII GGWCC 2 cut(s) 192, 942
BauI CACGAG 2 cut(s) 217, 581
BbvI GCAGC 2 cut(s) 442, 913
BccI CCATC 5 cut(s) 276, 333, 378, 680, 964
BceAI ACGGC 4 cut(s) 280, 461, 547, 1099
BciT130I CCWGG 1 cut(s) 964
BcnI CCSGG 4 cut(s) 460, 642, 867, 984
BcoDI GTCTC 4 cut(s) 488, 741, 746, 1131
BfaI CTAG 1 cut(s) 744
BfoI RGCGCY 2 cut(s) 456, 477
BisI GCNGC 7 cut(s) 246, 451, 456, 661, 664, 667, 927
BlsI GCNGC 7 cut(s) 247, 452, 457, 662, 665, 668, 928
Bme1390I CCNGG 5 cut(s) 460, 642, 867, 964, 984
Bme18I GGWCC 2 cut(s) 192, 942
BmeT110I CYCGRG 1 cut(s) 1115
BmgT120I GGNCC 4 cut(s) 192, 577, 942, 1085
BmiI GGNNCC 3 cut(s) 58, 193, 943
BmrFI CCNGG 5 cut(s) 460, 642, 867, 964, 984
BmrI ACTGGG 1 cut(s) 621
BmuI ACTGGG 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 1005
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 4 cut(s) 460, 642, 867, 984
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 2 cut(s) 488, 746
BsaJI CCNNGG 5 cut(s) 52, 866, 910, 963, 1116
BsaXI ACNNNNNCTCC 2 cut(s) 878, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 762
Bse118I RCCGGY 1 cut(s) 727
Bse1I ACTGG 1 cut(s) 616
BseBI CCWGG 1 cut(s) 964
BseDI CCNNGG 5 cut(s) 52, 866, 910, 963, 1116
BseGI GGATG 2 cut(s) 287, 691
BseLI CCNNNNNNNGG 2 cut(s) 189, 762
BseMII CTCAG 1 cut(s) 1019
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 1 cut(s) 142
BseX3I CGGCCG 1 cut(s) 729
BseXI GCAGC 2 cut(s) 442, 913
Bsh1285I CGRYCG 1 cut(s) 732
BshFI GGCC 6 cut(s) 118, 578, 731, 962, 1063, 1086
BsiEI CGRYCG 1 cut(s) 732
BsiHKCI CYCGRG 1 cut(s) 1115
BsiSI CCGG 6 cut(s) 459, 642, 657, 728, 866, 984
BslFI GGGAC 2 cut(s) 955, 966
BslI CCNNNNNNNGG 2 cut(s) 189, 762
BsmAI GTCTC 4 cut(s) 488, 741, 746, 1131
BsmFI GGGAC 2 cut(s) 955, 966
BsnI GGCC 6 cut(s) 118, 578, 731, 962, 1063, 1086
Bso31I GGTCTC 2 cut(s) 488, 746
BsoBI CYCGRG 1 cut(s) 1115
Bsp143I GATC 3 cut(s) 37, 589, 952
Bsp19I CCATGG 1 cut(s) 910
BspACI CCGC 9 cut(s) 153, 245, 450, 492, 603, 660, 663, 666, 725
BspANI GGCC 6 cut(s) 118, 578, 731, 962, 1063, 1086
BspCNI CTCAG 1 cut(s) 1018
BspLI GGNNCC 3 cut(s) 58, 193, 943
BspPI GGATC 1 cut(s) 947
BspTNI GGTCTC 2 cut(s) 488, 746
BsrFI RCCGGY 1 cut(s) 727
BsrI ACTGG 1 cut(s) 616
BssAI RCCGGY 1 cut(s) 727
BssECI CCNNGG 5 cut(s) 52, 866, 910, 963, 1116
BssMI GATC 3 cut(s) 37, 589, 952
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 2 cut(s) 217, 581
BssT1I CCWWGG 2 cut(s) 52, 910
Bst2BI CACGAG 2 cut(s) 217, 581
Bst2UI CCWGG 1 cut(s) 964
Bst4CI ACNGT 2 cut(s) 18, 1125
Bst6I CTCTTC 1 cut(s) 1041
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 522, 729
BstDEI CTNAG 2 cut(s) 1005, 1106
BstDSI CCRYGG 1 cut(s) 910
BstF5I GGATG 2 cut(s) 287, 691
BstH2I RGCGCY 2 cut(s) 456, 477
BstHHI GCGC 2 cut(s) 455, 476
BstKTI GATC 3 cut(s) 40, 592, 955
BstMAI GTCTC 4 cut(s) 488, 741, 746, 1131
BstMBI GATC 3 cut(s) 37, 589, 952
BstMCI CGRYCG 1 cut(s) 732
BstMWI GCNNNNNNNGC 3 cut(s) 666, 696, 993
BstNI CCWGG 1 cut(s) 964
BstSCI CCNGG 5 cut(s) 458, 640, 865, 962, 982
BstV1I GCAGC 2 cut(s) 442, 913
BstXI CCANNNNNNTGG 2 cut(s) 911, 963
BstZI CGGCCG 1 cut(s) 729
BsuRI GGCC 6 cut(s) 118, 578, 731, 962, 1063, 1086
BtgI CCRYGG 1 cut(s) 910
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 2 cut(s) 287, 691
BtsIMutI CAGTG 1 cut(s) 1121
Cac8I GCNNGC 2 cut(s) 522, 729
CfoI GCGC 2 cut(s) 455, 476
Cfr10I RCCGGY 1 cut(s) 727
Cfr13I GGNCC 4 cut(s) 192, 577, 942, 1085
CseI GACGC 2 cut(s) 266, 727
Csp6I GTAC 4 cut(s) 106, 565, 766, 1112
CviAII CATG 3 cut(s) 599, 793, 911
CviQI GTAC 4 cut(s) 106, 565, 766, 1112
DdeI CTNAG 2 cut(s) 1005, 1106
DpnI GATC 3 cut(s) 39, 591, 954
DpnII GATC 3 cut(s) 37, 589, 952
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 729
EagI CGGCCG 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 1041
EarI CTCTTC 1 cut(s) 1041
EciI GGCGGA 2 cut(s) 168, 714
EclXI CGGCCG 1 cut(s) 729
Eco130I CCWWGG 2 cut(s) 52, 910
Eco147I AGGCCT 1 cut(s) 118
Eco31I GGTCTC 2 cut(s) 488, 746
Eco32I GATATC 1 cut(s) 839
Eco47I GGWCC 2 cut(s) 192, 942
Eco52I CGGCCG 1 cut(s) 729
Eco57I CTGAAG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 1115
EcoRII CCWGG 1 cut(s) 962
EcoRV GATATC 1 cut(s) 839
EcoT14I CCWWGG 2 cut(s) 52, 910
ErhI CCWWGG 2 cut(s) 52, 910
FaeI CATG 3 cut(s) 602, 796, 914
FaiI YATR 6 cut(s) 143, 551, 600, 794, 912, 1139
FaqI GGGAC 2 cut(s) 955, 966
FatI CATG 3 cut(s) 598, 792, 910
FauI CCCGC 1 cut(s) 596
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 7 cut(s) 246, 451, 456, 661, 664, 667, 927
FokI GGATG 2 cut(s) 294, 698
Fsp4HI GCNGC 7 cut(s) 246, 451, 456, 661, 664, 667, 927
FspBI CTAG 1 cut(s) 744
GlaI GCGC 2 cut(s) 454, 475
GluI GCNGC 7 cut(s) 246, 451, 456, 661, 664, 667, 927
HaeII RGCGCY 2 cut(s) 456, 477
HaeIII GGCC 6 cut(s) 118, 578, 731, 962, 1063, 1086
HapII CCGG 6 cut(s) 459, 642, 657, 728, 866, 984
HgaI GACGC 2 cut(s) 266, 727
HhaI GCGC 2 cut(s) 455, 476
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 602, 796, 914
Hin6I GCGC 2 cut(s) 453, 474
HinP1I GCGC 2 cut(s) 453, 474
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 2 cut(s) 303, 405
HpaII CCGG 6 cut(s) 459, 642, 657, 728, 866, 984
HphI GGTGA 1 cut(s) 1064
Hpy166II GTNNAC 1 cut(s) 226
Hpy188III TCNNGA 2 cut(s) 808, 842
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 3 cut(s) 260, 437, 1085
HpyCH4III ACNGT 2 cut(s) 18, 1125
HpyCH4V TGCA 4 cut(s) 524, 780, 796, 1145
HpyF10VI GCNNNNNNNGC 3 cut(s) 666, 696, 993
HpyF3I CTNAG 2 cut(s) 1005, 1106
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 602, 796, 914
HspAI GCGC 2 cut(s) 453, 474
KroI GCCGGC 1 cut(s) 727
KroNI GCCGGC 1 cut(s) 729
Kzo9I GATC 3 cut(s) 37, 589, 952
LmnI GCTCC 3 cut(s) 62, 96, 814
Lsp1109I GCAGC 2 cut(s) 442, 913
MaeI CTAG 1 cut(s) 744
MaeIII GTNAC 1 cut(s) 990
MalI GATC 3 cut(s) 39, 591, 954
MboI GATC 3 cut(s) 37, 589, 952
MboII GAAGA 6 cut(s) 599, 628, 631, 661, 1058, 1061
MluCI AATT 9 cut(s) 61, 100, 133, 175, 525, 553, 915, 1065, 1091
MlyI GAGTC 6 cut(s) 57, 172, 209, 743, 998, 1046
MmeI TCCRAC 3 cut(s) 279, 1113, 1120
MroNI GCCGGC 1 cut(s) 727
MroXI GAANNNNTTC 3 cut(s) 290, 347, 392
MseI TTAA 2 cut(s) 174, 714
MspI CCGG 6 cut(s) 459, 642, 657, 728, 866, 984
MspR9I CCNGG 5 cut(s) 460, 642, 867, 964, 984
MvaI CCWGG 1 cut(s) 964
MwoI GCNNNNNNNGC 3 cut(s) 666, 696, 993
NaeI GCCGGC 1 cut(s) 729
NciI CCSGG 4 cut(s) 460, 642, 867, 984
NcoI CCATGG 1 cut(s) 910
NdeII GATC 3 cut(s) 37, 589, 952
NgoMIV GCCGGC 1 cut(s) 727
NlaIII CATG 3 cut(s) 602, 796, 914
NlaIV GGNNCC 3 cut(s) 58, 193, 943
NmeAIII GCCGAG 2 cut(s) 426, 757
NmuCI GTSAC 1 cut(s) 990
PceI AGGCCT 1 cut(s) 118
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 327, 429
PdiI GCCGGC 1 cut(s) 729
PdmI GAANNNNTTC 3 cut(s) 290, 347, 392
PfeI GAWTC 5 cut(s) 86, 324, 595, 802, 845
PkrI GCNGC 7 cut(s) 247, 452, 457, 662, 665, 668, 928
PleI GAGTC 6 cut(s) 56, 171, 209, 742, 997, 1046
PpsI GAGTC 6 cut(s) 56, 171, 209, 742, 997, 1046
Psp6I CCWGG 1 cut(s) 962
PspGI CCWGG 1 cut(s) 962
PspN4I GGNNCC 3 cut(s) 58, 193, 943
PspPI GGNCC 4 cut(s) 192, 577, 942, 1085
PsrI GAACNNNNNNTAC 2 cut(s) 1095, 1127
RsaI GTAC 4 cut(s) 107, 566, 767, 1113
RsaNI GTAC 4 cut(s) 106, 565, 766, 1112
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 174, 714
SatI GCNGC 7 cut(s) 246, 451, 456, 661, 664, 667, 927
Sau3AI GATC 3 cut(s) 37, 589, 952
Sau96I GGNCC 4 cut(s) 192, 577, 942, 1085
SchI GAGTC 6 cut(s) 57, 172, 209, 743, 998, 1046
ScrFI CCNGG 5 cut(s) 460, 642, 867, 964, 984
SinI GGWCC 2 cut(s) 192, 942
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 9 cut(s) 61, 100, 133, 175, 525, 553, 915, 1065, 1091
SseBI AGGCCT 1 cut(s) 118
SsiI CCGC 9 cut(s) 153, 245, 450, 492, 603, 660, 663, 666, 725
SspMI CTAG 1 cut(s) 744
StuI AGGCCT 1 cut(s) 118
StyD4I CCNGG 5 cut(s) 458, 640, 865, 962, 982
StyI CCWWGG 2 cut(s) 52, 910
TaaI ACNGT 2 cut(s) 18, 1125
TaqI TCGA 6 cut(s) 46, 89, 138, 225, 1012, 1044
TasI AATT 9 cut(s) 61, 100, 133, 175, 525, 553, 915, 1065, 1091
TauI GCSGC 5 cut(s) 248, 453, 663, 666, 669
TfiI GAWTC 5 cut(s) 86, 324, 595, 802, 845
Tru1I TTAA 2 cut(s) 174, 714
Tru9I TTAA 2 cut(s) 174, 714
TscAI CASTG 1 cut(s) 1128
TseFI GTSAC 1 cut(s) 990
TseI GCWGC 2 cut(s) 455, 926
Tsp45I GTSAC 1 cut(s) 990
TspDTI ATGAA 2 cut(s) 566, 587
TspGWI ACGGA 2 cut(s) 294, 336
TspRI CASTG 1 cut(s) 1128
VpaK11BI GGWCC 2 cut(s) 192, 942
XapI RAATTY 4 cut(s) 133, 553, 915, 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 3 cut(s) 290, 347, 392
XspI CTAG 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.