Rorug07G0013700

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0011698
Physical Location & Seq
Reverse (-)
1063227 .. 1065357
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0013700.1

Sequence Viewer

Length: 999 bp
ATGGACGTGTTGAGGCAAGTCAAATTAATTGGGGTGAAAATCGATCTAGACGAAGTTTCTCGCGCAAGGGCCATCGAGGCCATCGATTCGTTCCTCAAACAATACGAGGAATGTTTTTGGTCAATAGTGATCGCGGAGATCAGCACAGCGATCAGAGAAGAGCTCCTAAAATCTAGGAATGTGATCGACGTCGAGTTGGTTAGACAAGTTGTTGAGTATCTTTCAAACGGCGGCGTGGAGAAGAAGAAGGAGGAAGGTTTGGAGGAGGAGGATGGTTCGTACGAGGAGGGATTGGAGGAGGAAGATGTGGAAGACGAGGAGGGCTCGGAGGGATTGGGGGAGGAGTTGGAAGATGAAGAAGATTCGGATCAGGAGTCGGATGAGGATTCCGATGATGATGATGTGGAGGCGGTGGCGGAGGGGAAAACAGAGGTGGTGATGGATTATGAGGAGGAGGAGATTGATGATTTTAATGATCCAACAACGAGAGAAGAATGCATGGAGGATCTGTTGCTTGTGTGCAGTCGGATATATGGGATTGAGTGTAGCACCCTCAACGATTTGGAGACGGTGAAGAACGCCCTCAAGAGTAGCATTTTTCTAGAGGAATATGAGGACTGGAAAAAAGAGCAGACCATCACCAGTAGGGAAAAGGAGGATAAGGTTGATGTTAAGGACCAAGTTCTGAAACAGGAGGAGGAGGATAAGGCTGATGCTAAGGACCAGGTTCATCAACTGTTAAAGAATAATATCCTACCGATTAAAGAAGAAGGGATTGCTACCCACATGAACCAGATAAAACACTTAAGAAGAGAATTGTTACTTTCTCCTACAAGCCATATGGAATGTGGAGATTTGATAGAAATTGAAGAATGCGAGACTTTAACCACCTTGAACCTGCCAGAACAAGCAAAAGAAAGTAAGGGCAATGCTTATTCCTGCATCAAAGAAAGTTTGGAAAGGGGTGAAGCTAATAAGAGAAAGCTTGCCCAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

332

Amino Acids

38.02

Weight (kDa)

4.23

Isoelectric Point (pI)

63.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 906
AccII CGCG 2 cut(s) 63, 134
AciI CCGC 4 cut(s) 134, 231, 410, 416
AclWI GGATC 3 cut(s) 375, 470, 513
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 281
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 3 cut(s) 225, 869, 895
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 1 cut(s) 723
AluBI AGCT 3 cut(s) 163, 971, 985
AluI AGCT 3 cut(s) 163, 971, 985
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 560, 872
AlwI GGATC 3 cut(s) 375, 470, 513
AoxI GGCC 2 cut(s) 69, 78
AseI ATTAAT 1 cut(s) 26
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 3 cut(s) 69, 676, 721
AsuHPI GGTGA 5 cut(s) 46, 448, 583, 631, 977
AvaII GGWCC 2 cut(s) 676, 721
BanII GRGCYC 2 cut(s) 165, 326
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 5 cut(s) 80, 89, 266, 433, 644
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 1 cut(s) 725
BcoDI GTCTC 2 cut(s) 560, 872
BfaI CTAG 4 cut(s) 47, 174, 602, 997
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 1 cut(s) 906
BglI GCCNNNNNGGC 1 cut(s) 77
BglII AGATCT 1 cut(s) 993
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 2 cut(s) 676, 721
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 69, 676, 721
BmrFI CCNGG 1 cut(s) 725
BmsI GCATC 2 cut(s) 703, 951
BpiI GAAGAC 1 cut(s) 318
BplI GAGNNNNNCTC 4 cut(s) 147, 179, 308, 340
Bpu10I CCTNAGC 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 569
Bsa29I ATCGAT 2 cut(s) 42, 84
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bse1I ACTGG 2 cut(s) 623, 642
Bse3DI GCAATG 1 cut(s) 934
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 725
BseCI ATCGAT 2 cut(s) 42, 84
BseGI GGATG 2 cut(s) 277, 385
BseJI GATNNNNATC 1 cut(s) 366
BseMI GCAATG 1 cut(s) 934
BseNI ACTGG 2 cut(s) 623, 642
BsgI GTGCAG 1 cut(s) 541
Bsh1236I CGCG 2 cut(s) 63, 134
BshFI GGCC 2 cut(s) 71, 80
BshVI ATCGAT 2 cut(s) 42, 84
BsiHKAI GWGCWC 1 cut(s) 165
BsiWI CGTACG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 560, 872
BsmBI CGTCTC 1 cut(s) 560
BsmI GAATGC 2 cut(s) 500, 878
BsnI GGCC 2 cut(s) 71, 80
Bsp1286I GDGCHC 2 cut(s) 165, 326
Bsp143I GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
BspACI CCGC 4 cut(s) 134, 231, 410, 416
BspANI GGCC 2 cut(s) 71, 80
BspDI ATCGAT 2 cut(s) 42, 84
BspFNI CGCG 2 cut(s) 63, 134
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 3 cut(s) 375, 470, 513
BspQI GCTCTTC 1 cut(s) 153
BspTI CTTAAG 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 934
BsrI ACTGG 2 cut(s) 623, 642
BssMI GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 2 cut(s) 571, 738
Bst6I CTCTTC 2 cut(s) 153, 805
BstACI GRCGYC 1 cut(s) 189
BstAFI CTTAAG 1 cut(s) 805
BstC8I GCNNGC 1 cut(s) 987
BstDEI CTNAG 1 cut(s) 717
BstF5I GGATG 2 cut(s) 277, 385
BstFNI CGCG 2 cut(s) 63, 134
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 9 cut(s) 46, 132, 141, 153, 186, 370, 478, 508, 996
BstMAI GTCTC 2 cut(s) 560, 872
BstMBI GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 725
BstSCI CCNGG 1 cut(s) 723
BstUI CGCG 2 cut(s) 63, 134
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 2 cut(s) 505, 993
BstYI RGATCY 2 cut(s) 505, 993
Bsu15I ATCGAT 2 cut(s) 42, 84
BsuRI GGCC 2 cut(s) 71, 80
BsuTUI ATCGAT 2 cut(s) 42, 84
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 2 cut(s) 277, 385
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 1 cut(s) 987
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 69, 676, 721
ClaI ATCGAT 2 cut(s) 42, 84
CsiI ACCWGGT 1 cut(s) 723
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 499, 787
CviJI RGCY 8 cut(s) 71, 80, 163, 324, 710, 837, 971, 985
CviKI_1 RGCY 8 cut(s) 71, 80, 163, 324, 710, 837, 971, 985
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 717
DpnI GATC 9 cut(s) 45, 131, 140, 152, 185, 369, 477, 507, 995
DpnII GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
Eam1104I CTCTTC 2 cut(s) 153, 805
EarI CTCTTC 2 cut(s) 153, 805
EciI GGCGGA 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 165, 326
Eco47I GGWCC 2 cut(s) 676, 721
Eco53kI GAGCTC 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 500
EcoT38I GRGCYC 2 cut(s) 165, 326
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 2 cut(s) 502, 790
FaiI YATR 8 cut(s) 447, 500, 532, 534, 612, 788, 840, 842
FatI CATG 2 cut(s) 498, 786
FauNDI CATATG 1 cut(s) 840
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 2 cut(s) 284, 392
FriOI GRGCYC 2 cut(s) 165, 326
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 4 cut(s) 47, 174, 602, 997
GlaI GCGC 1 cut(s) 64
GluI GCNGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 71, 80
HhaI GCGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 502, 790
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 4 cut(s) 86, 362, 374, 386
HphI GGTGA 5 cut(s) 46, 448, 583, 631, 977
Hpy188I TCNGA 7 cut(s) 155, 328, 367, 379, 391, 528, 687
Hpy188III TCNNGA 4 cut(s) 47, 371, 586, 602
Hpy99I CGWCG 2 cut(s) 191, 194
HpyAV CCTTC 3 cut(s) 241, 248, 764
HpyCH4III ACNGT 2 cut(s) 571, 738
HpyCH4IV ACGT 2 cut(s) 6, 189
HpyCH4V TGCA 3 cut(s) 498, 522, 942
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 1 cut(s) 717
HpySE526I ACGT 2 cut(s) 6, 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 502, 790
HspAI GCGC 1 cut(s) 63
Kzo9I GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 168
LweI GCATC 2 cut(s) 703, 951
MabI ACCWGGT 1 cut(s) 723
MaeI CTAG 4 cut(s) 47, 174, 602, 997
MaeII ACGT 2 cut(s) 6, 189
MaeIII GTNAC 1 cut(s) 819
MalI GATC 9 cut(s) 45, 131, 140, 152, 185, 369, 477, 507, 995
MboI GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
MflI RGATCY 2 cut(s) 505, 993
MhlI GDGCHC 2 cut(s) 165, 326
MluCI AATT 4 cut(s) 23, 27, 815, 864
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 4 cut(s) 327, 357, 503, 506
Mph1103I ATGCAT 1 cut(s) 500
MseI TTAA 7 cut(s) 26, 471, 672, 740, 762, 806, 884
MspCI CTTAAG 1 cut(s) 805
MspR9I CCNGG 1 cut(s) 725
Mva1269I GAATGC 2 cut(s) 500, 878
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 2 cut(s) 63, 134
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeI CATATG 1 cut(s) 840
NdeII GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
NlaIII CATG 2 cut(s) 502, 790
NsiI ATGCAT 1 cut(s) 500
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 81
PctI GAATGC 2 cut(s) 500, 878
PfeI GAWTC 3 cut(s) 86, 362, 386
Pfl23II CGTACG 1 cut(s) 279
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 723
PspGI CCWGG 1 cut(s) 723
PspLI CGTACG 1 cut(s) 279
PspPI GGNCC 3 cut(s) 69, 676, 721
PsuI RGATCY 2 cut(s) 505, 993
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 7 cut(s) 26, 471, 672, 740, 762, 806, 884
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 9 cut(s) 43, 129, 138, 150, 183, 367, 475, 505, 993
Sau96I GGNCC 3 cut(s) 69, 676, 721
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 725
SduI GDGCHC 2 cut(s) 165, 326
SexAI ACCWGGT 1 cut(s) 723
SfaNI GCATC 2 cut(s) 703, 951
SfiI GGCCNNNNNGGCC 1 cut(s) 77
SinI GGWCC 2 cut(s) 676, 721
SmlI CTYRAG 2 cut(s) 584, 805
SmoI CTYRAG 2 cut(s) 584, 805
Sse9I AATT 4 cut(s) 23, 27, 815, 864
SsiI CCGC 4 cut(s) 134, 231, 410, 416
SspMI CTAG 4 cut(s) 47, 174, 602, 997
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 723
TaaI ACNGT 2 cut(s) 571, 738
TaiI ACGT 2 cut(s) 9, 192
TaqI TCGA 5 cut(s) 42, 75, 84, 186, 192
TasI AATT 4 cut(s) 23, 27, 815, 864
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 3 cut(s) 86, 362, 386
Tru1I TTAA 7 cut(s) 26, 471, 672, 740, 762, 806, 884
Tru9I TTAA 7 cut(s) 26, 471, 672, 740, 762, 806, 884
TspDTI ATGAA 3 cut(s) 369, 719, 803
Vha464I CTTAAG 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 676, 721
VspI ATTAAT 1 cut(s) 26
XbaI TCTAGA 2 cut(s) 46, 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XspI CTAG 4 cut(s) 47, 174, 602, 997
ZraI GACGTC 1 cut(s) 190
Zsp2I ATGCAT 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.