Rh7BG141500

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011698
Physical Location & Seq
Forward (+)
10478512 .. 10482036
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG141500.1

Sequence Viewer

Length: 999 bp
ATGGAGAACAACAACAACCAATCCTTCTGGCAATTCAGCGACCAGCTTCGTGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGCTCATACGGCAGCAAGAGGCCTGATGCGAGGAGGAACTTCGACATCAAGGTCGGCGGTGAGGTGAATTCTTTGATTAATTTGACCCCAAAAGGGTCTGATTTCAATGGGTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTCAGCGACAGGTTTAACAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTTAATGGGTTTAACCAGAAAGTCCCGGACTTCCATGGGTTTAATCAGAAGGTCCCGGACTTCAATGGGTTCAACCAGAAGGTTCCGGATTTTAACGGGTTTAATCAAGGGTGGAAACTCGGGTCTTCTGCTAATGGTGGCATCAATGGAGGCTTCAACAAGGGGATTTACTCCAAGCCTGCAATTCACAACAATAACATTAACCTGAACCTGAAGCCGTACAAGAACCAGGGTGAGGAGTTCGATCTTGGAGTTAAAGCTGGGAAGAAGAACAACAATAGCAACAAGAAGAAAAATGGTGATGACAATAACAAGGACGTCAAGGGTGACAAGAAATTCAAGACACTTCCACCGTCTGAGTCTCTCCCCAGAAATGAAACCATTGGTGGTTACATCTTCGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGCAGCTTTTTGGTTTGCCTCCACGTTACCGTGACTCAGTTCGGGCCATTACTCCAGGGTTGCCCCTTTTCCTTTACAACTACTCCACTCACCAACTCCATGGAATTTTTGAGGTTTGTGACTTTTTCTTTAGCATGATCTTATCTAATGTTGTGAAGAAGTTGGAATTTACTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

332

Amino Acids

37.46

Weight (kDa)

9.22

Isoelectric Point (pI)

21.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 257 - 312 5.4e-19 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 705
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 1 cut(s) 159
AcsI RAATTY 5 cut(s) 169, 293, 719, 924, 986
AcuI CTGAAG 3 cut(s) 303, 333, 617
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 4 cut(s) 195, 259, 260, 619
AjnI CCWGG 2 cut(s) 612, 874
AluBI AGCT 7 cut(s) 46, 105, 273, 317, 347, 644, 826
AluI AGCT 7 cut(s) 46, 105, 273, 317, 347, 644, 826
Alw26I GTCTC 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 503
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 2 cut(s) 122, 864
ApeKI GCWGC 3 cut(s) 102, 114, 823
ApoI RAATTY 5 cut(s) 169, 293, 719, 924, 986
AseI ATTAAT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 272
AspS9I GGNCC 3 cut(s) 246, 436, 864
AsuC2I CCSGG 2 cut(s) 410, 440
AsuHPI GGTGA 6 cut(s) 173, 178, 629, 695, 722, 902
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 2 cut(s) 246, 436
BbsI GAAGAC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 114, 126, 835
BceAI ACGGC 2 cut(s) 127, 586
BciT130I CCWGG 2 cut(s) 614, 876
BcnI CCSGG 2 cut(s) 410, 440
BcoDI GTCTC 1 cut(s) 750
BisI GCNGC 3 cut(s) 103, 115, 824
BlsI GCNGC 3 cut(s) 104, 116, 825
Bme1390I CCNGG 4 cut(s) 410, 440, 614, 876
Bme18I GGWCC 2 cut(s) 246, 436
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 3 cut(s) 246, 436, 864
BmiI GGNNCC 3 cut(s) 247, 438, 468
BmrFI CCNGG 4 cut(s) 410, 440, 614, 876
BmsI GCATC 2 cut(s) 118, 534
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 2 cut(s) 118, 858
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 2 cut(s) 410, 440
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 5 cut(s) 282, 418, 613, 875, 919
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 259, 260, 619
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 2 cut(s) 614, 876
BseDI CCNNGG 5 cut(s) 282, 418, 613, 875, 919
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 4 cut(s) 195, 259, 260, 619
BseMII CTCAG 2 cut(s) 732, 870
BseRI GAGGAG 2 cut(s) 148, 635
BseXI GCAGC 3 cut(s) 114, 126, 835
BseYI CCCAGC 1 cut(s) 644
BshFI GGCC 2 cut(s) 124, 866
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 3 cut(s) 410, 440, 470
BslFI GGGAC 2 cut(s) 392, 422
BslI CCNNNNNNNGG 4 cut(s) 195, 259, 260, 619
BsmAI GTCTC 1 cut(s) 750
BsmFI GGGAC 2 cut(s) 392, 422
BsnI GGCC 2 cut(s) 124, 866
BsoBI CYCGRG 1 cut(s) 503
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 3 cut(s) 93, 628, 957
Bsp19I CCATGG 2 cut(s) 418, 919
BspACI CCGC 1 cut(s) 159
BspANI GGCC 2 cut(s) 124, 866
BspCNI CTCAG 2 cut(s) 733, 869
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 247, 438, 468
BssECI CCNNGG 5 cut(s) 282, 418, 613, 875, 919
BssMI GATC 3 cut(s) 93, 628, 957
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 3 cut(s) 282, 418, 919
Bst2UI CCWGG 2 cut(s) 614, 876
Bst4CI ACNGT 2 cut(s) 738, 851
BstACI GRCGYC 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 2 cut(s) 741, 856
BstDSI CCRYGG 2 cut(s) 418, 919
BstF5I GGATG 1 cut(s) 59
BstKTI GATC 3 cut(s) 96, 631, 960
BstMAI GTCTC 1 cut(s) 750
BstMBI GATC 3 cut(s) 93, 628, 957
BstMWI GCNNNNNNNGC 2 cut(s) 111, 270
BstNI CCWGG 2 cut(s) 614, 876
BstSCI CCNGG 4 cut(s) 408, 438, 612, 874
BstV1I GCAGC 3 cut(s) 114, 126, 835
BstV2I GAAGAC 1 cut(s) 501
BstXI CCANNNNNNTGG 1 cut(s) 920
BsuRI GGCC 2 cut(s) 124, 866
BtgI CCRYGG 2 cut(s) 418, 919
BtsCI GGATG 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 564
Cfr13I GGNCC 3 cut(s) 246, 436, 864
Csp6I GTAC 1 cut(s) 604
CviAII CATG 4 cut(s) 419, 802, 920, 955
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 2 cut(s) 741, 856
DpnI GATC 3 cut(s) 95, 630, 959
DpnII GATC 3 cut(s) 93, 628, 957
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eco130I CCWWGG 3 cut(s) 282, 418, 919
Eco147I AGGCCT 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 246, 436
Eco57I CTGAAG 3 cut(s) 303, 333, 617
Eco88I CYCGRG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 436
EcoRI GAATTC 2 cut(s) 169, 293
EcoRII CCWGG 2 cut(s) 612, 874
EcoT14I CCWWGG 3 cut(s) 282, 418, 919
ErhI CCWWGG 3 cut(s) 282, 418, 919
FaeI CATG 4 cut(s) 422, 805, 923, 958
FaiI YATR 5 cut(s) 109, 420, 803, 921, 956
FaqI GGGAC 2 cut(s) 392, 422
FatI CATG 4 cut(s) 418, 801, 919, 954
Fnu4HI GCNGC 3 cut(s) 103, 115, 824
FokI GGATG 1 cut(s) 46
Fsp4HI GCNGC 3 cut(s) 103, 115, 824
GluI GCNGC 3 cut(s) 103, 115, 824
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 2 cut(s) 118, 858
HaeIII GGCC 2 cut(s) 124, 866
HapII CCGG 3 cut(s) 410, 440, 470
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 4 cut(s) 422, 805, 923, 958
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 2 cut(s) 315, 345
HinfI GANTC 4 cut(s) 89, 743, 811, 854
HpaII CCGG 3 cut(s) 410, 440, 470
HphI GGTGA 6 cut(s) 173, 178, 629, 695, 722, 902
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 8 cut(s) 202, 250, 292, 322, 352, 382, 432, 742
Hpy188III TCNNGA 5 cut(s) 55, 97, 470, 724, 817
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 34, 215, 427, 457
HpyCH4III ACNGT 2 cut(s) 738, 851
HpyCH4IV ACGT 2 cut(s) 702, 844
HpyCH4V TGCA 3 cut(s) 566, 789, 805
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 270
HpyF3I CTNAG 2 cut(s) 741, 856
HpySE526I ACGT 2 cut(s) 702, 844
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 4 cut(s) 422, 805, 923, 958
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 93, 628, 957
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 3 cut(s) 114, 126, 835
LweI GCATC 2 cut(s) 118, 534
MaeII ACGT 2 cut(s) 702, 844
MaeIII GTNAC 5 cut(s) 710, 773, 845, 851, 938
MalI GATC 3 cut(s) 95, 630, 959
MboI GATC 3 cut(s) 93, 628, 957
MboII GAAGA 6 cut(s) 501, 661, 664, 685, 772, 988
MlyI GAGTC 3 cut(s) 83, 752, 848
MmeI TCCRAC 2 cut(s) 273, 963
MroI TCCGGA 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 9 cut(s) 180, 309, 387, 396, 426, 477, 486, 585, 639
MspI CCGG 3 cut(s) 410, 440, 470
MspR9I CCNGG 4 cut(s) 410, 440, 614, 876
MvaI CCWGG 2 cut(s) 614, 876
MwoI GCNNNNNNNGC 2 cut(s) 111, 270
NciI CCSGG 2 cut(s) 410, 440
NcoI CCATGG 2 cut(s) 418, 919
NdeII GATC 3 cut(s) 93, 628, 957
NlaIII CATG 4 cut(s) 422, 805, 923, 958
NlaIV GGNNCC 3 cut(s) 247, 438, 468
NmuCI GTSAC 3 cut(s) 710, 851, 938
PceI AGGCCT 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 811
PfoI TCCNGGA 2 cut(s) 408, 438
PkrI GCNGC 3 cut(s) 104, 116, 825
PleI GAGTC 3 cut(s) 83, 751, 848
PpsI GAGTC 3 cut(s) 83, 751, 848
PpuMI RGGWCCY 1 cut(s) 436
PshBI ATTAAT 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 436
Psp6I CCWGG 2 cut(s) 612, 874
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 2 cut(s) 612, 874
PspN4I GGNNCC 3 cut(s) 247, 438, 468
PspPI GGNCC 3 cut(s) 246, 436, 864
PspPPI RGGWCCY 1 cut(s) 436
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
SaqAI TTAA 9 cut(s) 180, 309, 387, 396, 426, 477, 486, 585, 639
SatI GCNGC 3 cut(s) 103, 115, 824
Sau3AI GATC 3 cut(s) 93, 628, 957
Sau96I GGNCC 3 cut(s) 246, 436, 864
SchI GAGTC 3 cut(s) 83, 752, 848
ScrFI CCNGG 4 cut(s) 410, 440, 614, 876
SfaNI GCATC 2 cut(s) 118, 534
SinI GGWCC 2 cut(s) 246, 436
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SseBI AGGCCT 1 cut(s) 124
SsiI CCGC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 4 cut(s) 408, 438, 612, 874
StyI CCWWGG 3 cut(s) 282, 418, 919
TaaI ACNGT 2 cut(s) 738, 851
TaiI ACGT 2 cut(s) 705, 847
TaqI TCGA 3 cut(s) 92, 144, 627
TfiI GAWTC 1 cut(s) 811
Tru1I TTAA 9 cut(s) 180, 309, 387, 396, 426, 477, 486, 585, 639
Tru9I TTAA 9 cut(s) 180, 309, 387, 396, 426, 477, 486, 585, 639
TseFI GTSAC 3 cut(s) 710, 851, 938
TseI GCWGC 3 cut(s) 102, 114, 823
Tsp45I GTSAC 3 cut(s) 710, 851, 938
TspDTI ATGAA 2 cut(s) 234, 774
TspGWI ACGGA 1 cut(s) 70
VpaK11BI GGWCC 2 cut(s) 246, 436
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 5 cut(s) 169, 293, 719, 924, 986
XmnI GAANNNNTTC 1 cut(s) 272
ZraI GACGTC 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.