MD06G1110600.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011698
Physical Location & Seq
Reverse (-)
24959875 .. 24962922
3048 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1110600.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGAGAACAACAACCAGTCGTTTTGGCAGTTCAGCGACCAGCTTCGAGTTCAAGGTTCCAATTTCGCAAATCTGAACATCAACGATTCGATTTGGAGCAATTCGTACAGCAAGAGGCCCGATGAGAGGAGGAATTTCGATATAAAGGTCGGTGGCGAAGTGAGTCCGATCGTTAATTTGACCCCGAAGGGGTCCGGTTTCAACGGGTTCAACGACTCGTGGGTCGATAATCTGAAGCCGAAAGCCGCGTTTTCCGACGCGAACGGCAGTTCCAACGACGGCTGGAACAGCTTCAAGCCGAAAGCTTCCGAATTGAACAGTGGATTCAACGACAACAGGTGGAACAGCTTCAAGCCAAAAGCTTCGGACTTCAACAACGGTTTTAACGACGGGTGGAAACTCGGCGGCGCCGCCGTGTCGCAGAATACCACCGTTGGTCTCAATGGCGGTTTCAACAAGGGGATTTACTCCAAGCCTGCAATTCACAACAATAACAACAACAACAACAACAATGCTATGAACTTGAAGCCTTACAAGAACAAGGCTCACGAGGATGATCAGATGCATGGGGGGAAAATTGGGAAGAAGAACAACAATAACAGCAATCCCGGCAAGAAGAAATCCGGCAGCGGCGGCGATGATAATAACAAGGATGGAAAGAGTAGCGTGGATAAGAGGTTTAAGACGCTTCCGCCGGCCGAGTCTCTGCCGAGAAATGAAACCATTGGTGGATATATCTTTGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGCAGCTTTTCGGTTTGCCTCCACGTTATCGTGATTCAGTGAGGGCGATAACTCCGGGGTTGCCCCTTTTCCTCTACAACTACTCTACCCATCAACTCCATGGAATTTTTGAGGCTGCAAGTTTTGGAGGTACCAACTTTGATCCATCGGCCTGGGAGGACAAGAAGTGTCCCGGCGAGTCACGTTTCCCGGCTCAGGTTCGAGTTTTTACTCGTAAAGTCTGTGAGCCACTTGAAGAGGACTCATTCAGGCCAATTCTTCACCACTACGATGGCCCTAAATTCCGACTTGAGCTTAGCGTACCTGAGGCACTGTCTCTGTTAGATATATTTGCAGACCAAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

376

Amino Acids

41.78

Weight (kDa)

8.88

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 243 - 370 8.2e-40 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
Acc65I GGTACC 1 cut(s) 908
AccB1I GGYRCC 2 cut(s) 407, 908
AccII CGCG 2 cut(s) 248, 260
AciI CCGC 7 cut(s) 246, 405, 411, 447, 630, 633, 692
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 3 cut(s) 133, 882, 1058
AcuI CTGAAG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 3 cut(s) 107, 910, 1080
AfiI CCNNNNNNNGG 3 cut(s) 126, 189, 973
AjnI CCWGG 1 cut(s) 929
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 7 cut(s) 43, 291, 305, 348, 362, 784, 1072
AluI AGCT 7 cut(s) 43, 291, 305, 348, 362, 784, 1072
Alw26I GTCTC 3 cut(s) 443, 708, 1098
AlwI GGATC 1 cut(s) 914
AoxI GGCC 5 cut(s) 116, 696, 927, 1028, 1051
ApeKI GCWGC 3 cut(s) 627, 781, 893
ApoI RAATTY 3 cut(s) 133, 882, 1058
Asp700I GAANNNNTTC 2 cut(s) 290, 347
Asp718I GGTACC 1 cut(s) 908
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 3 cut(s) 117, 192, 1052
AsuC2I CCSGG 4 cut(s) 609, 834, 951, 968
AsuHPI GGTGA 1 cut(s) 1031
AvaII GGWCC 1 cut(s) 192
AxyI CCTNAGG 1 cut(s) 1083
BanI GGYRCC 2 cut(s) 407, 908
BauI CACGAG 2 cut(s) 217, 548
BbvI GCAGC 3 cut(s) 639, 793, 880
BccI CCATC 4 cut(s) 647, 876, 931, 1043
BceAI ACGGC 3 cut(s) 280, 295, 398
BciT130I CCWGG 1 cut(s) 931
BclI TGATCA 1 cut(s) 556
BcnI CCSGG 4 cut(s) 609, 834, 951, 968
BcoDI GTCTC 3 cut(s) 443, 708, 1098
BfoI RGCGCY 1 cut(s) 411
BisI GCNGC 8 cut(s) 246, 406, 411, 628, 631, 634, 782, 894
BlpI GCTNAGC 1 cut(s) 1073
BlsI GCNGC 8 cut(s) 247, 407, 412, 629, 632, 635, 783, 895
Bme1390I CCNGG 5 cut(s) 609, 834, 931, 951, 968
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 117, 192, 1052
BmiI GGNNCC 4 cut(s) 58, 193, 409, 910
BmrFI CCNGG 5 cut(s) 609, 834, 931, 951, 968
BmsI GCATC 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 972
Bpu1102I GCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 758, 1088
BpuMI CCSGG 4 cut(s) 609, 834, 951, 968
BsaHI GRCGYC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 833, 877, 930
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 189, 973
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 1 cut(s) 16
Bse21I CCTNAGG 1 cut(s) 1083
BseBI CCWGG 1 cut(s) 931
BseDI CCNNGG 3 cut(s) 833, 877, 930
BseGI GGATG 2 cut(s) 559, 658
BseLI CCNNNNNNNGG 3 cut(s) 126, 189, 973
BseMII CTCAG 2 cut(s) 986, 1074
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 142
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 3 cut(s) 639, 793, 880
Bsh1236I CGCG 2 cut(s) 248, 260
Bsh1285I CGRYCG 2 cut(s) 171, 699
BshFI GGCC 5 cut(s) 118, 698, 929, 1030, 1053
BshNI GGYRCC 2 cut(s) 407, 908
BsiEI CGRYCG 2 cut(s) 171, 699
BsiSI CCGG 7 cut(s) 195, 609, 624, 695, 833, 951, 968
BslFI GGGAC 1 cut(s) 933
BslI CCNNNNNNNGG 3 cut(s) 126, 189, 973
BsmAI GTCTC 3 cut(s) 443, 708, 1098
BsmFI GGGAC 1 cut(s) 933
BsnI GGCC 5 cut(s) 118, 698, 929, 1030, 1053
Bso31I GGTCTC 1 cut(s) 443
Bsp143I GATC 3 cut(s) 168, 556, 919
Bsp1720I GCTNAGC 1 cut(s) 1073
Bsp19I CCATGG 1 cut(s) 877
BspACI CCGC 7 cut(s) 246, 405, 411, 447, 630, 633, 692
BspANI GGCC 5 cut(s) 118, 698, 929, 1030, 1053
BspCNI CTCAG 2 cut(s) 985, 1075
BspFNI CGCG 2 cut(s) 248, 260
BspLI GGNNCC 4 cut(s) 58, 193, 409, 910
BspPI GGATC 1 cut(s) 914
BspT107I GGYRCC 2 cut(s) 407, 908
BspTNI GGTCTC 1 cut(s) 443
BsrFI RCCGGY 1 cut(s) 694
BsrI ACTGG 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 3 cut(s) 833, 877, 930
BssMI GATC 3 cut(s) 168, 556, 919
BssNI GRCGYC 1 cut(s) 408
BssSI CACGAG 2 cut(s) 217, 548
BssT1I CCWWGG 1 cut(s) 877
Bst2BI CACGAG 2 cut(s) 217, 548
Bst2UI CCWGG 1 cut(s) 931
Bst4CI ACNGT 4 cut(s) 320, 380, 433, 1092
Bst6I CTCTTC 1 cut(s) 1008
BstACI GRCGYC 1 cut(s) 408
BstC8I GCNNGC 2 cut(s) 477, 696
BstDEI CTNAG 3 cut(s) 972, 1073, 1083
BstDSI CCRYGG 1 cut(s) 877
BstF5I GGATG 2 cut(s) 559, 658
BstFNI CGCG 2 cut(s) 248, 260
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 3 cut(s) 171, 559, 922
BstMAI GTCTC 3 cut(s) 443, 708, 1098
BstMBI GATC 3 cut(s) 168, 556, 919
BstMCI CGRYCG 2 cut(s) 171, 699
BstMWI GCNNNNNNNGC 3 cut(s) 288, 609, 633
BstNI CCWGG 1 cut(s) 931
BstSCI CCNGG 5 cut(s) 607, 832, 929, 949, 966
BstUI CGCG 2 cut(s) 248, 260
BstV1I GCAGC 3 cut(s) 639, 793, 880
BstXI CCANNNNNNTGG 2 cut(s) 930, 1049
BstZI CGGCCG 1 cut(s) 696
Bsu36I CCTNAGG 1 cut(s) 1083
BsuRI GGCC 5 cut(s) 118, 698, 929, 1030, 1053
BtgI CCRYGG 1 cut(s) 877
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 2 cut(s) 559, 658
BtsIMutI CAGTG 3 cut(s) 325, 822, 1088
Cac8I GCNNGC 2 cut(s) 477, 696
CfoI GCGC 1 cut(s) 410
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 3 cut(s) 117, 192, 1052
CseI GACGC 2 cut(s) 266, 694
Csp6I GTAC 3 cut(s) 106, 909, 1079
CviAII CATG 3 cut(s) 566, 760, 878
CviQI GTAC 3 cut(s) 106, 909, 1079
DdeI CTNAG 3 cut(s) 972, 1073, 1083
DinI GGCGCC 1 cut(s) 409
DpnI GATC 3 cut(s) 170, 558, 921
DpnII GATC 3 cut(s) 168, 556, 919
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 696
EagI CGGCCG 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 1008
EarI CTCTTC 1 cut(s) 1008
EciI GGCGGA 1 cut(s) 681
EclXI CGGCCG 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 877
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 192
Eco52I CGGCCG 1 cut(s) 696
Eco57I CTGAAG 1 cut(s) 254
Eco81I CCTNAGG 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 877
EcoT22I ATGCAT 1 cut(s) 567
EgeI GGCGCC 1 cut(s) 409
EheI GGCGCC 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 877
FaeI CATG 3 cut(s) 569, 763, 881
FaiI YATR 7 cut(s) 143, 518, 567, 735, 761, 879, 1106
FaqI GGGAC 1 cut(s) 933
FatI CATG 3 cut(s) 565, 759, 877
FbaI TGATCA 1 cut(s) 556
Fnu4HI GCNGC 8 cut(s) 246, 406, 411, 628, 631, 634, 782, 894
FokI GGATG 2 cut(s) 566, 665
Fsp4HI GCNGC 8 cut(s) 246, 406, 411, 628, 631, 634, 782, 894
GlaI GCGC 1 cut(s) 409
GluI GCNGC 8 cut(s) 246, 406, 411, 628, 631, 634, 782, 894
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 5 cut(s) 118, 698, 929, 1030, 1053
HapII CCGG 7 cut(s) 195, 609, 624, 695, 833, 951, 968
HgaI GACGC 2 cut(s) 266, 694
HhaI GCGC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 3 cut(s) 569, 763, 881
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HindIII AAGCTT 2 cut(s) 303, 360
HinfI GANTC 9 cut(s) 86, 163, 215, 324, 701, 769, 812, 956, 1019
HpaII CCGG 7 cut(s) 195, 609, 624, 695, 833, 951, 968
HphI GGTGA 1 cut(s) 1031
Hpy188I TCNGA 8 cut(s) 75, 168, 234, 256, 310, 367, 561, 1064
Hpy188III TCNNGA 3 cut(s) 548, 775, 809
Hpy99I CGWCG 3 cut(s) 260, 281, 392
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 4 cut(s) 320, 380, 433, 1092
HpyCH4IV ACGT 2 cut(s) 802, 961
HpyCH4V TGCA 6 cut(s) 479, 565, 747, 763, 896, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 609, 633
HpyF3I CTNAG 3 cut(s) 972, 1073, 1083
HpySE526I ACGT 2 cut(s) 802, 961
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 3 cut(s) 569, 763, 881
HspAI GCGC 1 cut(s) 408
KasI GGCGCC 1 cut(s) 407
KpnI GGTACC 1 cut(s) 912
KroI GCCGGC 1 cut(s) 694
KroNI GCCGGC 1 cut(s) 696
Ksp22I TGATCA 1 cut(s) 556
Kzo9I GATC 3 cut(s) 168, 556, 919
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 3 cut(s) 639, 793, 880
LweI GCATC 1 cut(s) 552
MaeII ACGT 2 cut(s) 802, 961
MaeIII GTNAC 1 cut(s) 957
MalI GATC 3 cut(s) 170, 558, 921
MboI GATC 3 cut(s) 168, 556, 919
MboII GAAGA 5 cut(s) 595, 598, 628, 1025, 1028
Mly113I GGCGCC 1 cut(s) 408
MlyI GAGTC 5 cut(s) 172, 209, 710, 965, 1013
MmeI TCCRAC 3 cut(s) 279, 297, 1087
Mph1103I ATGCAT 1 cut(s) 567
MroNI GCCGGC 1 cut(s) 694
MroXI GAANNNNTTC 2 cut(s) 290, 347
MseI TTAA 3 cut(s) 174, 384, 681
MslI CAYNNNNRTG 2 cut(s) 552, 1047
MspA1I CMGCKG 1 cut(s) 630
MspI CCGG 7 cut(s) 195, 609, 624, 695, 833, 951, 968
MspR9I CCNGG 5 cut(s) 609, 834, 931, 951, 968
MvaI CCWGG 1 cut(s) 931
MvnI CGCG 2 cut(s) 248, 260
MwoI GCNNNNNNNGC 3 cut(s) 288, 609, 633
NaeI GCCGGC 1 cut(s) 696
NarI GGCGCC 1 cut(s) 408
NciI CCSGG 4 cut(s) 609, 834, 951, 968
NcoI CCATGG 1 cut(s) 877
NdeII GATC 3 cut(s) 168, 556, 919
NgoMIV GCCGGC 1 cut(s) 694
NlaIII CATG 3 cut(s) 569, 763, 881
NlaIV GGNNCC 4 cut(s) 58, 193, 409, 910
NmeAIII GCCGAG 3 cut(s) 381, 724, 735
NmuCI GTSAC 1 cut(s) 957
NsiI ATGCAT 1 cut(s) 567
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 384
PdiI GCCGGC 1 cut(s) 696
PdmI GAANNNNTTC 2 cut(s) 290, 347
PfeI GAWTC 4 cut(s) 86, 324, 769, 812
PkrI GCNGC 8 cut(s) 247, 407, 412, 629, 632, 635, 783, 895
Ple19I CGATCG 1 cut(s) 171
PleI GAGTC 5 cut(s) 171, 209, 709, 964, 1013
PluTI GGCGCC 1 cut(s) 411
PpsI GAGTC 5 cut(s) 171, 209, 709, 964, 1013
Psp6I CCWGG 1 cut(s) 929
PspGI CCWGG 1 cut(s) 929
PspN4I GGNNCC 4 cut(s) 58, 193, 409, 910
PspPI GGNCC 3 cut(s) 117, 192, 1052
PvuI CGATCG 1 cut(s) 171
RsaI GTAC 3 cut(s) 107, 910, 1080
RsaNI GTAC 3 cut(s) 106, 909, 1079
RseI CAYNNNNRTG 2 cut(s) 552, 1047
SaqAI TTAA 3 cut(s) 174, 384, 681
SatI GCNGC 8 cut(s) 246, 406, 411, 628, 631, 634, 782, 894
Sau3AI GATC 3 cut(s) 168, 556, 919
Sau96I GGNCC 3 cut(s) 117, 192, 1052
SchI GAGTC 5 cut(s) 172, 209, 710, 965, 1013
ScrFI CCNGG 5 cut(s) 609, 834, 931, 951, 968
SfaNI GCATC 1 cut(s) 552
SfoI GGCGCC 1 cut(s) 409
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 2 cut(s) 552, 1047
SmlI CTYRAG 2 cut(s) 773, 1067
SmoI CTYRAG 2 cut(s) 773, 1067
SsiI CCGC 7 cut(s) 246, 405, 411, 447, 630, 633, 692
SspDI GGCGCC 1 cut(s) 407
StyD4I CCNGG 5 cut(s) 607, 832, 929, 949, 966
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 4 cut(s) 320, 380, 433, 1092
TaiI ACGT 2 cut(s) 805, 964
TaqI TCGA 5 cut(s) 46, 89, 138, 225, 979
TauI GCSGC 5 cut(s) 248, 408, 413, 633, 636
TfiI GAWTC 4 cut(s) 86, 324, 769, 812
Tru1I TTAA 3 cut(s) 174, 384, 681
Tru9I TTAA 3 cut(s) 174, 384, 681
TscAI CASTG 3 cut(s) 325, 822, 1095
TseFI GTSAC 1 cut(s) 957
TseI GCWGC 3 cut(s) 627, 781, 893
Tsp45I GTSAC 1 cut(s) 957
TspDTI ATGAA 2 cut(s) 533, 732
TspRI CASTG 3 cut(s) 325, 822, 1095
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 3 cut(s) 133, 882, 1058
XcmI CCANNNNNNNNNTGG 1 cut(s) 875
XmnI GAANNNNTTC 2 cut(s) 290, 347
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.