RLG00000004218

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011698
Physical Location & Seq
Reverse (-)
57347355 .. 57349163
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004218

Sequence Viewer

Length: 1233 bp
ATGGAGAACAACAACAACAACCAATCCTTCTGGCAATTCAGCGACCAGCTTCGGGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGTTCCTACGGCAGCAAGAGGCCTGATGCGAGGAGGAACTTCGATATCAAGGTCGGCGGTGAGGTGAATTCTTTGATTAATTTGACCCCAAAAGGGTCTGATTTCAATGGGTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTGAACGGGTTCAGCGACAGGTTTAACAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTATCAGACTTCAATTCTTTCAACCCAAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTTAATGGGTTTAACCAGAAAATCCCGGATTTCAATGGGTTTAATCAGAAGGTCCCGGACTTCAATGGATTCAACCAGAAGGTTCCGGATTTTAACGGGTTTAATGAAGGGTGGAAACTCGGGGCTTCTGCTAATGGAGCCATCAATGGAGGCTTCAACAAGGGGATTTACTCCAAGCCTGCAATTCACAACAATAACATTAACCTGAACCTGAAGCCGTACAAGAACCAGGGTGAGGAGTTTGATCTTGGGGTTAAAGCTGGGAAGAAGAACAACAATAGCAACAAGAAGAAAAATGGTGATGACAATAACAAGGATGTCAAGGGTGACAAGAAATTCAAGACACTTCCACCGTCTGAGTCTCTCCCCAGAAATGAAACCATTGGTGGTTACATCTTCGTCTGCAACAATGATACCATGCAAGAGAATCTCAAGAGGCAGCTTTTTGGTTTGCCTCCACGTTACCGTGACTCAGTTCGGGCCATTACTCCAGGGTTGCCCCTTTTCCTTTACAACTACTCCACTCACCAACTCCATGGAATTTTTGAGGCTGCAAGCTTTGGAGGAACCAACTTTGATCCATCTGCTTGGGAAGACAAGAAATGCCCTGGTGAATCGCGCTTCCCTGCTCAGGTGAGAGTTCTAACTAGGAAAGTCTGTGAACCACTGGAAGAGGATTCCTTCAGGCCAATTCTTCATCACTACGATGGCCCTAAATTCCGTCTTGAACTCAGCGTGCCAGAGGCCCTCTCTCTGTTGGATATATTTGCAGACCAAGATGATATATTTGTAACTGAAACCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

411

Amino Acids

46.1

Weight (kDa)

6.97

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 271 - 398 8.7e-40 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1045
AccIII TCCGGA 1 cut(s) 511
AciI CCGC 1 cut(s) 162
AclWI GGATC 1 cut(s) 998
AcsI RAATTY 4 cut(s) 172, 761, 966, 1142
AcuI CTGAAG 4 cut(s) 315, 375, 659, 1093
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 6 cut(s) 52, 198, 262, 263, 661, 1057
AjnI CCWGG 3 cut(s) 654, 916, 1033
AluBI AGCT 7 cut(s) 49, 276, 329, 389, 686, 868, 984
AluI AGCT 7 cut(s) 49, 276, 329, 389, 686, 868, 984
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 545
Aor13HI TCCGGA 1 cut(s) 511
AoxI GGCC 5 cut(s) 125, 906, 1112, 1135, 1170
ApeKI GCWGC 3 cut(s) 117, 865, 977
ApoI RAATTY 4 cut(s) 172, 761, 966, 1142
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 2 cut(s) 275, 305
AspLEI GCGC 1 cut(s) 1047
AspS9I GGNCC 6 cut(s) 55, 249, 478, 906, 1136, 1171
AsuC2I CCSGG 2 cut(s) 452, 482
AsuHPI GGTGA 8 cut(s) 176, 181, 671, 737, 764, 944, 1049, 1072
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 3 cut(s) 55, 249, 478
BbsI GAAGAC 1 cut(s) 1026
BbvI GCAGC 3 cut(s) 129, 877, 964
BccI CCATC 3 cut(s) 575, 1015, 1127
BceAI ACGGC 2 cut(s) 130, 628
BciT130I CCWGG 3 cut(s) 656, 918, 1035
BcnI CCSGG 2 cut(s) 452, 482
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 1 cut(s) 1074
BisI GCNGC 3 cut(s) 118, 866, 978
BlsI GCNGC 3 cut(s) 119, 867, 979
Bme1390I CCNGG 5 cut(s) 452, 482, 656, 918, 1035
Bme18I GGWCC 3 cut(s) 55, 249, 478
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 6 cut(s) 55, 249, 478, 906, 1136, 1171
BmiI GGNNCC 6 cut(s) 56, 250, 480, 510, 565, 994
BmrFI CCNGG 5 cut(s) 452, 482, 656, 918, 1035
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 1026
BplI GAGNNNNNCTC 2 cut(s) 1160, 1192
BpmI CTGGAG 2 cut(s) 121, 900
Bpu10I CCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 1 cut(s) 842
BpuMI CCSGG 2 cut(s) 452, 482
BsaJI CCNNGG 5 cut(s) 285, 655, 917, 961, 1033
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 929, 959
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 198, 262, 263, 661, 1057
Bse1I ACTGG 1 cut(s) 1098
BseAI TCCGGA 1 cut(s) 511
BseBI CCWGG 3 cut(s) 656, 918, 1035
BseDI CCNNGG 5 cut(s) 285, 655, 917, 961, 1033
BseGI GGATG 2 cut(s) 62, 748
BseLI CCNNNNNNNGG 6 cut(s) 52, 198, 262, 263, 661, 1057
BseMII CTCAG 4 cut(s) 774, 912, 1070, 1171
BseNI ACTGG 1 cut(s) 1098
BseRI GAGGAG 2 cut(s) 151, 677
BseXI GCAGC 3 cut(s) 129, 877, 964
BseYI CCCAGC 1 cut(s) 686
Bsh1236I CGCG 1 cut(s) 1045
BshFI GGCC 5 cut(s) 127, 908, 1114, 1137, 1172
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 3 cut(s) 452, 482, 512
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 6 cut(s) 52, 198, 262, 263, 661, 1057
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 1 cut(s) 464
BsnI GGCC 5 cut(s) 127, 908, 1114, 1137, 1172
BsoBI CYCGRG 1 cut(s) 545
Bsp13I TCCGGA 1 cut(s) 511
Bsp143I GATC 3 cut(s) 96, 670, 1003
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 1 cut(s) 162
BspANI GGCC 5 cut(s) 127, 908, 1114, 1137, 1172
BspCNI CTCAG 4 cut(s) 775, 911, 1069, 1170
BspEI TCCGGA 1 cut(s) 511
BspFNI CGCG 1 cut(s) 1045
BspLI GGNNCC 6 cut(s) 56, 250, 480, 510, 565, 994
BspPI GGATC 1 cut(s) 998
BsrI ACTGG 1 cut(s) 1098
BssECI CCNNGG 5 cut(s) 285, 655, 917, 961, 1033
BssMI GATC 3 cut(s) 96, 670, 1003
BssT1I CCWWGG 2 cut(s) 285, 961
Bst2UI CCWGG 3 cut(s) 656, 918, 1035
Bst4CI ACNGT 2 cut(s) 780, 893
Bst6I CTCTTC 1 cut(s) 1092
BstC8I GCNNGC 3 cut(s) 606, 982, 1163
BstDEI CTNAG 4 cut(s) 783, 898, 1056, 1157
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 2 cut(s) 62, 748
BstFNI CGCG 1 cut(s) 1045
BstHHI GCGC 1 cut(s) 1047
BstKTI GATC 3 cut(s) 99, 673, 1006
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 3 cut(s) 96, 670, 1003
BstMWI GCNNNNNNNGC 2 cut(s) 273, 563
BstNI CCWGG 3 cut(s) 656, 918, 1035
BstSCI CCNGG 5 cut(s) 450, 480, 654, 916, 1033
BstUI CGCG 1 cut(s) 1045
BstV1I GCAGC 3 cut(s) 129, 877, 964
BstV2I GAAGAC 1 cut(s) 1026
BstXI CCANNNNNNTGG 2 cut(s) 962, 1014
BsuRI GGCC 5 cut(s) 127, 908, 1114, 1137, 1172
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 2 cut(s) 62, 748
BtsIMutI CAGTG 1 cut(s) 1091
Cac8I GCNNGC 3 cut(s) 606, 982, 1163
CfoI GCGC 1 cut(s) 1047
Cfr13I GGNCC 6 cut(s) 55, 249, 478, 906, 1136, 1171
Csp6I GTAC 1 cut(s) 646
CviAII CATG 2 cut(s) 844, 962
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 4 cut(s) 783, 898, 1056, 1157
DpnI GATC 3 cut(s) 98, 672, 1005
DpnII GATC 3 cut(s) 96, 670, 1003
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
Eco130I CCWWGG 2 cut(s) 285, 961
Eco147I AGGCCT 1 cut(s) 127
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 3 cut(s) 55, 249, 478
Eco57I CTGAAG 4 cut(s) 315, 375, 659, 1093
Eco88I CYCGRG 1 cut(s) 545
EcoO109I RGGNCCY 2 cut(s) 478, 1171
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 654, 916, 1033
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 285, 961
ErhI CCWWGG 2 cut(s) 285, 961
FaeI CATG 2 cut(s) 847, 965
FaiI YATR 4 cut(s) 845, 963, 1190, 1211
FaqI GGGAC 1 cut(s) 464
FatI CATG 2 cut(s) 843, 961
Fnu4HI GCNGC 3 cut(s) 118, 866, 978
FokI GGATG 2 cut(s) 49, 755
Fsp4HI GCNGC 3 cut(s) 118, 866, 978
FspBI CTAG 1 cut(s) 1074
GlaI GCGC 1 cut(s) 1046
GluI GCNGC 3 cut(s) 118, 866, 978
GsaI CCCAGC 1 cut(s) 690
GsuI CTGGAG 2 cut(s) 121, 900
HaeIII GGCC 5 cut(s) 127, 908, 1114, 1137, 1172
HapII CCGG 3 cut(s) 452, 482, 512
HhaI GCGC 1 cut(s) 1047
Hin1II CATG 2 cut(s) 847, 965
Hin6I GCGC 1 cut(s) 1045
HinP1I GCGC 1 cut(s) 1045
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 3 cut(s) 327, 387, 982
HinfI GANTC 7 cut(s) 92, 495, 785, 853, 896, 1040, 1103
HpaII CCGG 3 cut(s) 452, 482, 512
HphI GGTGA 8 cut(s) 176, 181, 671, 737, 764, 944, 1049, 1072
Hpy166II GTNNAC 2 cut(s) 88, 1088
Hpy188I TCNGA 9 cut(s) 205, 253, 295, 334, 364, 394, 424, 474, 784
Hpy188III TCNNGA 6 cut(s) 58, 100, 512, 766, 859, 1151
Hpy8I GTNNAC 2 cut(s) 88, 1088
HpyAV CCTTC 6 cut(s) 37, 218, 469, 499, 527, 1117
HpyCH4III ACNGT 2 cut(s) 780, 893
HpyCH4IV ACGT 1 cut(s) 886
HpyCH4V TGCA 5 cut(s) 608, 831, 847, 980, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 563
HpyF3I CTNAG 4 cut(s) 783, 898, 1056, 1157
HpySE526I ACGT 1 cut(s) 886
Hsp92II CATG 2 cut(s) 847, 965
HspAI GCGC 1 cut(s) 1045
Kpn2I TCCGGA 1 cut(s) 511
Kzo9I GATC 3 cut(s) 96, 670, 1003
LmnI GCTCC 2 cut(s) 102, 563
Lsp1109I GCAGC 3 cut(s) 129, 877, 964
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 1 cut(s) 886
MaeIII GTNAC 5 cut(s) 752, 815, 887, 893, 1216
MalI GATC 3 cut(s) 98, 672, 1005
MboI GATC 3 cut(s) 96, 670, 1003
MboII GAAGA 7 cut(s) 703, 706, 727, 814, 1031, 1109, 1112
MlyI GAGTC 3 cut(s) 86, 794, 890
MmeI TCCRAC 2 cut(s) 276, 1164
MroI TCCGGA 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 275, 305
MseI TTAA 9 cut(s) 183, 321, 429, 438, 468, 519, 528, 627, 681
MslI CAYNNNNRTG 1 cut(s) 1131
MspI CCGG 3 cut(s) 452, 482, 512
MspR9I CCNGG 5 cut(s) 452, 482, 656, 918, 1035
MvaI CCWGG 3 cut(s) 656, 918, 1035
MvnI CGCG 1 cut(s) 1045
MwoI GCNNNNNNNGC 2 cut(s) 273, 563
NciI CCSGG 2 cut(s) 452, 482
NcoI CCATGG 1 cut(s) 961
NdeII GATC 3 cut(s) 96, 670, 1003
NlaIII CATG 2 cut(s) 847, 965
NlaIV GGNNCC 6 cut(s) 56, 250, 480, 510, 565, 994
NmuCI GTSAC 2 cut(s) 752, 893
PceI AGGCCT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 2 cut(s) 275, 305
PfeI GAWTC 4 cut(s) 495, 853, 1040, 1103
PfoI TCCNGGA 2 cut(s) 450, 480
PkrI GCNGC 3 cut(s) 119, 867, 979
PleI GAGTC 3 cut(s) 86, 793, 890
PpsI GAGTC 3 cut(s) 86, 793, 890
PpuMI RGGWCCY 1 cut(s) 478
PshBI ATTAAT 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 3 cut(s) 654, 916, 1033
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 3 cut(s) 654, 916, 1033
PspN4I GGNNCC 6 cut(s) 56, 250, 480, 510, 565, 994
PspPI GGNCC 6 cut(s) 55, 249, 478, 906, 1136, 1171
PspPPI RGGWCCY 1 cut(s) 478
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 1131
SaqAI TTAA 9 cut(s) 183, 321, 429, 438, 468, 519, 528, 627, 681
SatI GCNGC 3 cut(s) 118, 866, 978
Sau3AI GATC 3 cut(s) 96, 670, 1003
Sau96I GGNCC 6 cut(s) 55, 249, 478, 906, 1136, 1171
SchI GAGTC 3 cut(s) 86, 794, 890
ScrFI CCNGG 5 cut(s) 452, 482, 656, 918, 1035
SfaNI GCATC 1 cut(s) 121
SinI GGWCC 3 cut(s) 55, 249, 478
SmiMI CAYNNNNRTG 1 cut(s) 1131
SmlI CTYRAG 1 cut(s) 857
SmoI CTYRAG 1 cut(s) 857
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 1 cut(s) 162
SspMI CTAG 1 cut(s) 1074
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 5 cut(s) 450, 480, 654, 916, 1033
StyI CCWWGG 2 cut(s) 285, 961
TaaI ACNGT 2 cut(s) 780, 893
TaiI ACGT 1 cut(s) 889
TaqI TCGA 2 cut(s) 95, 147
TfiI GAWTC 4 cut(s) 495, 853, 1040, 1103
Tru1I TTAA 9 cut(s) 183, 321, 429, 438, 468, 519, 528, 627, 681
Tru9I TTAA 9 cut(s) 183, 321, 429, 438, 468, 519, 528, 627, 681
TscAI CASTG 1 cut(s) 1098
TseFI GTSAC 2 cut(s) 752, 893
TseI GCWGC 3 cut(s) 117, 865, 977
Tsp45I GTSAC 2 cut(s) 752, 893
TspDTI ATGAA 4 cut(s) 237, 546, 816, 1112
TspGWI ACGGA 2 cut(s) 73, 1136
TspRI CASTG 1 cut(s) 1098
VpaK11BI GGWCC 3 cut(s) 55, 249, 478
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 4 cut(s) 172, 761, 966, 1142
XmnI GAANNNNTTC 2 cut(s) 275, 305
XspI CTAG 1 cut(s) 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.