FvH4_5g08070

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004286
Physical Location & Seq
Reverse (-)
4701628 .. 4710921
9294 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08070.t1

Sequence Viewer

Length: 3651 bp
ATGGTTTCAACGAGGAGAAGTGGATCTCTCTCCGGCAACAACAATAACAACAGCAAAAGATCGTCGTCCTCGGACGACAACAACAACAAGCCTCAGTCTCCGAAGCGTCAGAAGGTCGATAACAATGGTGTTGCTTCCGAGAAAACCGAGAATTCCAAGGAGGTTTGTACTCCGGCCACGGCGGCGGCTGATCCCGGAGAATGCAGCGCCGCCGGCGAGGCTCCGGCGGCCGGGGATGGTGTGACTTCTGTAAAGACTGAAGCTTCTGCACAAGCTGTGGCGGTGACTCCGCCGCGTGTGGCTGAAGGGACTTCTCCAGCTGCTGTGCTGGATAAGGCGAGGAGTTCCATTTTGGCATGGAGTTCGTATCAGAAGTCGGGTCTGAATTCGGCGAGTTTTGAGCTGGCAACACCTTGGTGCAAGCTTCTGTCACAGTCAGCTCTGAATCTGAACATTGCTATCACAACATCAAATTTCACTATTGGTTCGAGCAGAAGCAGCAATTTCCCTCTGAAGGATAGTACTATTAGTGCATGTCTGTGCAAGATCAGACGCGAACAGCGGGAGGGCGGTGCTGTTACGATTATAGAAAGTATGGGAAACAAGGGATCACTGCAAGTAAATGGGGCACATGTGAAGAAAGGCAACAGCTGTGTGCTCAACTCAGGTGATGAAGTGGTATTTGGTCCTCTGGGAAATCATGCTTATATTTTTCAACTACTTGTGCCTGAGGGTCCAGTTAAGGGTACAGAGGTTCCAAGTGGCATTGGAAAATATCTGCTTGACAGGAGAGCCGGAGATCCTTCAGCTGTGGATGGGGCTTCCATACTGGCTTCTCTTAGTATGAGACCAGAACTACCACGATGGAAGTCTGCAGCGCAGGCCACCAATAAATTCCATCCAGGGGCTGACGCACCAGCTCAGTCTGTTATTCCAGAAGGTACAGAAGGTGAGCTTGAAGAACTCGAAGACAGCTTGACTCCAAACAGAGCTACTGACAAAACTGAAGAGATTGGAGGAATCAACAAGAATCTTACTCCAGAATGCAACCCAGATTCGGGCATAGAGGCAGACAATGTATTGGAAGAAAGAAATGAGTGGACGAGGGATTCGATGCCAGCATCAACGTCAGGCATGTCTGTGCGATGTGCTGTTTTTAAAGAAGGCATTCATGCAGCAATTCTTGAAGGCAATAGCGTGGATGTTTCATTTGATAATTTCCCGTACTATTTGAGTGAGAATACGAAGAATGTGCTGATTGCAGCTTCATTTATACACTTGAAACATAAAGAACATGCAAAGTATACTTCTGAGCTTACCACTGTGAATCCTCGAATATTGCTATCTGGTCCTGCAGGGTCTGAGATATACCAGGAGATGTTGGCTAAGGCACTTGCTCAATATTTCGGGGCAAAGTTGCTCATATTTGATAGCCACTCACTTTTGGGTGGTTTATTGTCAAAGGAAGCCGAGCTGCTCAAGGATGGTTCTAATGGAGATAAAATGAGCACCTTGGCCAAACCAAGTCCTGTTCCTACAGACTTGGCTAAGAGTATTGATCCTTCAGCTTCTGAGCTAGAGGCACCAAAGTTGGCGACAAGCTCTTCAACTGGGCCTTCATATGGCCTCGAATCCCAGCCTAAGAAGGAGACTGACGGCATCGCCACATCCTCAGGGACATCTAAGAATTACGTGTTTAAAATAGGTGACAGAGTAAGGTTCATAGGCATATCTTCTAGTGGCTTATATCCTACATCTTCTTCACGGGGCCCAGCGACTGGAGCCCGAGGAGAGGTTATGTTGGTTTTTGAGGACAATCCTTTGTCAAAAATTGGTGTGAGGTTTGATAAACCAATACTTGATGGTGTTGATCTCGGGGGTCTTTGTAAAGGACATGGATTCTTTTGCAACGCCTCTGATCTTCGTTTGGAGAACACGGGTGGGGATGATCTGGATAGATTACTTATCAATACATTGTTTGAGGCTGTACATAGTGAGAGCAGAAGCTCTCCTTTCATTTTGTTCATGAAAGATGCGGAGAAGTCCCTTGTAGGAAATCAGGATTCATATTCCACATTCAGAGGCAGGCTTGATAAGCTTCCTGATAATGTGGTTGTTATTGGCTCTCATACTCATACTGACAATCGCAAGGAGAAGTCGCATCCTGGTGGTCTATTATTCACAAAGTTCGGTAGCAATCAAACTGCTCTTCTTGACTTGGCTTTTCCGGATAGTTTTGGAAGACTGCACGAAAGAGGGAAGGAAGTTCCCAAGGCAACAAAACTTCTGACGAAACTTTTTCCCAATAAAGTAACCATTCATATGCCACAGGATGAGGCACTTCTTGTCTCATGGAAACTGCATTTGGATCGTGATACCGAAACCCTCAAGATGAAGGGAAATCTAAACCACTTGCGCGCTGTTCTGGGTCGGTGTGGGATGGAATGCGAAGGACTTGATACACTAAGCATCAAGGACCAGACGCTCACAAATGAAAGTTCAGAGAAGGTAGTTGGATGGGCGTTAAGCCATCATTTAATGCAGAATCCTGAAGCTGATCCAGAAACAAAAGTAGTTTTGTCTGCTGAGAGCATCCAGTACGGACTTGGAATCTTGCAGTCTATCCAGAATGAAAATAAGAGCTTGAAGAAGTCACTTAAGGATGTTGTAACAGAAAATGAATTTGAGAAAAGGCTTTTGGCTGATGTTATCCCACCAACTGACATTGGAGTCACGTTTGATGATATTGGGGCACTTGAAAATGTGAAGGATACATTGAAAGAATTGGTGATGCTGCCTTTACAAAGGCCGGAGCTTTTCTGCAAGGGGCAATTAACCAAGCCTTGCAAAGGCATCCTGTTATTTGGGCCCCCTGGGACAGGCAAGACGATGCTGGCAAAGGCTGTGGCTACAGAAGCTGGTGCAAACTTCATTAACATATCCATGTCGAGCATCACATCTAAGTGGTTTGGTGAGGGCGAGAAGTATGTGAAAGCTGTTTTCTCTCTAGCTAGTAAAATTGCCCCTAGTGTTGTATTTGTTGATGAAGTTGACAGCATGTTGGGCCGGCGGGAAAACCCAGGGGAGCATGAGGCAATGCGCAAGATGAAAAATGAATTTATGGTAAATTGGGATGGGTTACGAACAAAAGAAGCAGAACGAGTTCTGGTACTTGCAGCCACAAATAGGCCGTTTGACCTTGATGAAGCTGTCATCCGAAGGCTGCCCCGTAGGTTAATGGTAAATTTGCCAGATGCTCCCAATAGAGCGAAGATATTAAGAGTCATATTGGCAAAAGAGGACTTATCTCCCGATGTTGATTTTGAGGCAATTGCGAGCATGTCAGATGGATATTCTGGAAGTGATCTTAAAAATCTATGCGTGGCTGCTGCACACCATCCAATTAAAGAGATATTGGAAAAGGAAAAGAAGGATCGTGCTGCAGCTATAGCAGAAGGTAAACCTGTTCCAGCATTGAGTAGCAGTGGTGATGTTCGGCCTGTAAACATGGATGACTTTAAATATGCTCATGAACAGGTATGTGCCAGTGTATCCTCAGAGTCTGTAAACATGACAGAGCTCTTACAATGGAATGAACTATATGGAGAAGGGGGTTCTAGAAGAAAGAAGGCCCTTAGTTACTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

1217

Amino Acids

131.28

Weight (kDa)

5.97

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 160 - 229 2.9e-06 FHA domain
DUF7751 PF24933 814 - 873 3.7e-19 Domain of unknown function (DUF7751)
AAA PF00004 952 - 1082 1.4e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 1105 - 1141 4.8e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2729
Acc16I TGCGCA 1 cut(s) 3101
AccB1I GGYRCC 1 cut(s) 1582
AccB7I CCANNNNNTGG 1 cut(s) 1778
AccI GTMKAC 1 cut(s) 1304
AccII CGCG 3 cut(s) 295, 555, 2418
AccIII TCCGGA 1 cut(s) 2227
AclWI GGATC 8 cut(s) 31, 185, 616, 794, 1553, 2376, 2552, 3441
AcoI YGGCCR 3 cut(s) 174, 228, 1515
AcsI RAATTY 7 cut(s) 151, 385, 472, 893, 2681, 3116, 3244
AcuI CTGAAG 7 cut(s) 279, 324, 533, 789, 1026, 1548, 2571
AfaI GTAC 8 cut(s) 169, 523, 748, 943, 1226, 1989, 2600, 3171
AflII CTTAAG 1 cut(s) 2657
AflIII ACRYGT 2 cut(s) 631, 1692
AgsI TTSAA 9 cut(s) 9, 716, 959, 1187, 1282, 1608, 2647, 2759, 2779
AjnI CCWGG 5 cut(s) 901, 1371, 2164, 2872, 3079
AjuI GAANNNNNNNTTGG 6 cut(s) 666, 698, 2348, 2380, 2681, 2713
AleI CACNNNNGTG 1 cut(s) 415
Alw21I GWGCWC 3 cut(s) 660, 1511, 3582
Alw26I GTCTC 4 cut(s) 102, 841, 1643, 2353
AlwI GGATC 8 cut(s) 31, 185, 616, 794, 1553, 2376, 2552, 3441
AlwNI CAGNNNCTG 7 cut(s) 323, 908, 1361, 1571, 1778, 2918, 3563
Ama87I CYCGRG 2 cut(s) 1785, 1874
Aor13HI TCCGGA 1 cut(s) 2227
ApaI GGGCCC 2 cut(s) 1772, 2871
ApoI RAATTY 7 cut(s) 151, 385, 472, 893, 2681, 3116, 3244
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
Asp700I GAANNNNTTC 2 cut(s) 1167, 3162
AspLEI GCGC 5 cut(s) 209, 880, 2418, 2420, 3102
AsuC2I CCSGG 2 cut(s) 195, 232
AsuHPI GGTGA 7 cut(s) 295, 680, 962, 1718, 2800, 2984, 3500
AvaI CYCGRG 2 cut(s) 1785, 1874
AvaII GGWCC 4 cut(s) 686, 734, 1349, 2476
AxyI CCTNAGG 2 cut(s) 729, 1672
BaeGI GKGCMC 4 cut(s) 631, 1772, 2755, 2871
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BalI TGGCCA 1 cut(s) 1517
BanI GGYRCC 1 cut(s) 1582
BanII GRGCYC 4 cut(s) 1772, 1786, 2871, 3582
BarI GAAGNNNNNNTAC 2 cut(s) 1546, 1578
BbsI GAAGAC 2 cut(s) 975, 2248
Bbv12I GWGCWC 3 cut(s) 660, 1511, 3582
BceAI ACGGC 3 cut(s) 195, 1672, 3175
BcgI CGANNNNNNTGC 6 cut(s) 345, 379, 1234, 1268, 2351, 2385
BciT130I CCWGG 5 cut(s) 903, 1373, 2166, 2874, 3081
BciVI GTATCC 2 cut(s) 2764, 3562
BcnI CCSGG 2 cut(s) 195, 232
BcoDI GTCTC 4 cut(s) 102, 841, 1643, 2353
BfaI CTAG 6 cut(s) 1577, 1737, 3008, 3012, 3027, 3618
BfmI CTRYAG 6 cut(s) 873, 1353, 1536, 2910, 3441, 3447
BfoI RGCGCY 1 cut(s) 210
BfrI CTTAAG 1 cut(s) 2657
BfuI GTATCC 2 cut(s) 2764, 3562
BglI GCCNNNNNGGC 2 cut(s) 182, 218
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 7 cut(s) 195, 232, 903, 1373, 2166, 2874, 3081
Bme18I GGWCC 4 cut(s) 686, 734, 1349, 2476
BmeT110I CYCGRG 2 cut(s) 1785, 1874
BmiI GGNNCC 9 cut(s) 222, 735, 756, 1584, 1769, 1770, 1783, 2869, 2870
BmrFI CCNGG 7 cut(s) 195, 232, 903, 1373, 2166, 2874, 3081
BmrI ACTGGG 1 cut(s) 1620
BmuI ACTGGG 1 cut(s) 1620
BpiI GAAGAC 2 cut(s) 975, 2248
BpmI CTGGAG 3 cut(s) 300, 1023, 1800
Bpu10I CCTNAGC 1 cut(s) 1386
BpuEI CTTGAG 2 cut(s) 1463, 2372
BpuMI CCSGG 2 cut(s) 195, 232
BsaAI YACGTR 1 cut(s) 1693
BsaI GGTCTC 1 cut(s) 841
BsaWI WCCGGW 1 cut(s) 2227
Bse118I RCCGGY 2 cut(s) 212, 3066
Bse1I ACTGG 6 cut(s) 737, 834, 1615, 1783, 2596, 3546
Bse21I CCTNAGG 2 cut(s) 729, 1672
Bse3DI GCAATG 2 cut(s) 453, 3102
BseAI TCCGGA 1 cut(s) 2227
BseBI CCWGG 5 cut(s) 903, 1373, 2166, 2874, 3081
BseMI GCAATG 2 cut(s) 453, 3102
BseNI ACTGG 6 cut(s) 737, 834, 1615, 1783, 2596, 3546
BsePI GCGCGC 1 cut(s) 2416
BseRI GAGGAG 3 cut(s) 28, 355, 1803
BseSI GKGCMC 4 cut(s) 631, 1772, 2755, 2871
BseX3I CGGCCG 1 cut(s) 228
BseYI CCCAGC 2 cut(s) 1635, 1771
BsgI GTGCAG 3 cut(s) 252, 2231, 3375
Bsh1236I CGCG 3 cut(s) 295, 555, 2418
Bsh1285I CGRYCG 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 1582
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 3 cut(s) 660, 1511, 3582
BsiHKCI CYCGRG 2 cut(s) 1785, 1874
BslFI GGGAC 4 cut(s) 322, 1690, 2029, 2890
BsmAI GTCTC 4 cut(s) 102, 841, 1643, 2353
BsmFI GGGAC 4 cut(s) 322, 1690, 2029, 2890
BsmI GAATGC 4 cut(s) 206, 1049, 1167, 2450
Bso31I GGTCTC 1 cut(s) 841
BsoBI CYCGRG 2 cut(s) 1785, 1874
Bsp120I GGGCCC 2 cut(s) 1768, 2867
Bsp1286I GDGCHC 8 cut(s) 631, 660, 1511, 1772, 1786, 2755, 2871, 3582
Bsp13I TCCGGA 1 cut(s) 2227
Bsp1407I TGTACA 1 cut(s) 1987
BspEI TCCGGA 1 cut(s) 2227
BspFNI CGCG 3 cut(s) 295, 555, 2418
BspHI TCATGA 2 cut(s) 2025, 3529
BspLI GGNNCC 9 cut(s) 222, 735, 756, 1584, 1769, 1770, 1783, 2869, 2870
BspMAI CTGCAG 3 cut(s) 877, 1357, 3445
BspPI GGATC 8 cut(s) 31, 185, 616, 794, 1553, 2376, 2552, 3441
BspQI GCTCTTC 2 cut(s) 1609, 2214
BspT107I GGYRCC 1 cut(s) 1582
BspTI CTTAAG 1 cut(s) 2657
BspTNI GGTCTC 1 cut(s) 841
BsrDI GCAATG 2 cut(s) 453, 3102
BsrFI RCCGGY 2 cut(s) 212, 3066
BsrGI TGTACA 1 cut(s) 1987
BsrI ACTGG 6 cut(s) 737, 834, 1615, 1783, 2596, 3546
BssAI RCCGGY 2 cut(s) 212, 3066
BssHII GCGCGC 1 cut(s) 2416
BssNAI GTATAC 1 cut(s) 1305
BssT1I CCWWGG 4 cut(s) 156, 413, 1512, 2271
Bst1107I GTATAC 1 cut(s) 1305
Bst2UI CCWGG 5 cut(s) 903, 1373, 2166, 2874, 3081
Bst4CI ACNGT 2 cut(s) 435, 1324
Bst6I CTCTTC 3 cut(s) 1002, 1609, 2214
BstAFI CTTAAG 1 cut(s) 2657
BstAUI TGTACA 1 cut(s) 1987
BstBAI YACGTR 1 cut(s) 1693
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 2 cut(s) 2847, 2877
BstFNI CGCG 3 cut(s) 295, 555, 2418
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 5 cut(s) 209, 880, 2418, 2420, 3102
BstMAI GTCTC 4 cut(s) 102, 841, 1643, 2353
BstMCI CGRYCG 1 cut(s) 231
BstNI CCWGG 5 cut(s) 903, 1373, 2166, 2874, 3081
BstNSI RCATGY 6 cut(s) 537, 635, 1138, 1298, 3061, 3343
BstSCI CCNGG 7 cut(s) 193, 230, 901, 1371, 2164, 2872, 3079
BstSFI CTRYAG 6 cut(s) 873, 1353, 1536, 2910, 3441, 3447
BstSLI GKGCMC 4 cut(s) 631, 1772, 2755, 2871
BstUI CGCG 3 cut(s) 295, 555, 2418
BstV2I GAAGAC 2 cut(s) 975, 2248
BstX2I RGATCY 2 cut(s) 23, 799
BstYI RGATCY 2 cut(s) 23, 799
BstZ17I GTATAC 1 cut(s) 1305
BstZI CGGCCG 1 cut(s) 228
Bsu36I CCTNAGG 2 cut(s) 729, 1672
BsuI GTATCC 2 cut(s) 2764, 3562
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 2 cut(s) 1159, 1645
BtsI GCAGTG 2 cut(s) 611, 3490
BtsIMutI CAGTG 4 cut(s) 611, 1320, 3490, 3553
CaiI CAGNNNCTG 7 cut(s) 323, 908, 1361, 1571, 1778, 2918, 3563
CciI TCATGA 2 cut(s) 2025, 3529
CfoI GCGC 5 cut(s) 209, 880, 2418, 2420, 3102
Cfr10I RCCGGY 2 cut(s) 212, 3066
CseI GACGC 4 cut(s) 95, 561, 920, 2491
Csp6I GTAC 8 cut(s) 168, 522, 747, 942, 1225, 1988, 2599, 3170
CspCI CAANNNNNGTGG 2 cut(s) 2886, 2921
CviQI GTAC 8 cut(s) 168, 522, 747, 942, 1225, 1988, 2599, 3170
DraI TTTAAA 3 cut(s) 1159, 1699, 3520
DrdI GACNNNNNNGTC 1 cut(s) 2729
DseDI GACNNNNNNGTC 1 cut(s) 2729
EaeI YGGCCR 3 cut(s) 174, 228, 1515
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 3 cut(s) 1002, 1609, 2214
EarI CTCTTC 3 cut(s) 1002, 1609, 2214
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 3580
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 4 cut(s) 156, 413, 1512, 2271
Eco24I GRGCYC 4 cut(s) 1772, 1786, 2871, 3582
Eco31I GGTCTC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 686, 734, 1349, 2476
Eco52I CGGCCG 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 3580
Eco57I CTGAAG 7 cut(s) 279, 324, 533, 789, 1026, 1548, 2571
Eco81I CCTNAGG 2 cut(s) 729, 1672
Eco88I CYCGRG 2 cut(s) 1785, 1874
EcoICRI GAGCTC 1 cut(s) 3580
EcoNI CCTNNNNNAGG 2 cut(s) 2847, 2877
EcoO109I RGGNCCY 3 cut(s) 1768, 2868, 3631
EcoRI GAATTC 2 cut(s) 151, 385
EcoRII CCWGG 5 cut(s) 901, 1371, 2164, 2872, 3079
EcoT14I CCWWGG 4 cut(s) 156, 413, 1512, 2271
EcoT38I GRGCYC 4 cut(s) 1772, 1786, 2871, 3582
ErhI CCWWGG 4 cut(s) 156, 413, 1512, 2271
FalI AAGNNNNNCTT 4 cut(s) 939, 971, 1996, 2028
FaqI GGGAC 4 cut(s) 322, 1690, 2029, 2890
FauI CCCGC 2 cut(s) 555, 3063
FauNDI CATATG 2 cut(s) 1621, 2322
FblI GTMKAC 1 cut(s) 1304
FriOI GRGCYC 4 cut(s) 1772, 1786, 2871, 3582
FspBI CTAG 6 cut(s) 1577, 1737, 3008, 3012, 3027, 3618
FspI TGCGCA 1 cut(s) 3101
GlaI GCGC 5 cut(s) 208, 879, 2417, 2419, 3101
GsaI CCCAGC 2 cut(s) 1639, 1775
GsuI CTGGAG 3 cut(s) 300, 1023, 1800
HaeII RGCGCY 1 cut(s) 210
HgaI GACGC 4 cut(s) 95, 561, 920, 2491
HhaI GCGC 5 cut(s) 209, 880, 2418, 2420, 3102
Hin6I GCGC 5 cut(s) 207, 878, 2416, 2418, 3100
HinP1I GCGC 5 cut(s) 207, 878, 2416, 2418, 3100
HincII GTYRAC 1 cut(s) 3052
HindII GTYRAC 1 cut(s) 3052
HindIII AAGCTT 3 cut(s) 261, 422, 2096
HphI GGTGA 7 cut(s) 295, 680, 962, 1718, 2800, 2984, 3500
Hpy166II GTNNAC 6 cut(s) 1101, 1305, 3052, 3461, 3505, 3568
Hpy8I GTNNAC 6 cut(s) 1101, 1305, 3052, 3461, 3505, 3568
Hpy99I CGWCG 1 cut(s) 67
HpyCH4III ACNGT 2 cut(s) 435, 1324
HpyCH4IV ACGT 3 cut(s) 1127, 1692, 2735
HpySE526I ACGT 3 cut(s) 1127, 1692, 2735
HspAI GCGC 5 cut(s) 207, 878, 2416, 2418, 3100
Kpn2I TCCGGA 1 cut(s) 2227
KroI GCCGGC 2 cut(s) 212, 3066
KroNI GCCGGC 2 cut(s) 214, 3068
LguI GCTCTTC 2 cut(s) 1609, 2214
LmnI GCTCC 5 cut(s) 226, 1781, 2812, 3085, 3262
MaeI CTAG 6 cut(s) 1577, 1737, 3008, 3012, 3027, 3618
MaeII ACGT 3 cut(s) 1127, 1692, 2735
MfeI CAATTG 1 cut(s) 3330
MflI RGATCY 2 cut(s) 23, 799
MhlI GDGCHC 8 cut(s) 631, 660, 1511, 1772, 1786, 2755, 2871, 3582
MlsI TGGCCA 1 cut(s) 1517
MluNI TGGCCA 1 cut(s) 1517
MlyI GAGTC 5 cut(s) 280, 973, 2739, 3291, 3569
MmeI TCCRAC 1 cut(s) 2494
Mox20I TGGCCA 1 cut(s) 1517
MreI CGCCGGCG 1 cut(s) 212
MroI TCCGGA 1 cut(s) 2227
MroNI GCCGGC 2 cut(s) 212, 3066
MroXI GAANNNNTTC 2 cut(s) 1167, 3162
MscI TGGCCA 1 cut(s) 1517
MslI CAYNNNNRTG 7 cut(s) 415, 538, 1139, 2166, 2321, 2942, 2962
Msp20I TGGCCA 1 cut(s) 1517
MspA1I CMGCKG 4 cut(s) 320, 562, 651, 809
MspCI CTTAAG 1 cut(s) 2657
MspR9I CCNGG 7 cut(s) 195, 232, 903, 1373, 2166, 2874, 3081
MunI CAATTG 1 cut(s) 3330
Mva1269I GAATGC 4 cut(s) 206, 1049, 1167, 2450
MvaI CCWGG 5 cut(s) 903, 1373, 2166, 2874, 3081
MvnI CGCG 3 cut(s) 295, 555, 2418
NaeI GCCGGC 2 cut(s) 214, 3068
NciI CCSGG 2 cut(s) 195, 232
NdeI CATATG 2 cut(s) 1621, 2322
NgoMIV GCCGGC 2 cut(s) 212, 3066
NlaIV GGNNCC 9 cut(s) 222, 735, 756, 1584, 1769, 1770, 1783, 2869, 2870
NmeAIII GCCGAG 1 cut(s) 1495
NmuCI GTSAC 6 cut(s) 241, 283, 429, 1706, 2652, 2731
NsbI TGCGCA 1 cut(s) 3101
NspI RCATGY 6 cut(s) 537, 635, 1138, 1298, 3061, 3343
OliI CACNNNNGTG 1 cut(s) 415
PagI TCATGA 2 cut(s) 2025, 3529
PasI CCCWGGG 2 cut(s) 2873, 3080
PauI GCGCGC 1 cut(s) 2416
PciI ACATGT 1 cut(s) 631
PciSI GCTCTTC 2 cut(s) 1609, 2214
PcsI WCGNNNNNNNCGW 1 cut(s) 1109
PctI GAATGC 4 cut(s) 206, 1049, 1167, 2450
PdiI GCCGGC 2 cut(s) 214, 3068
PdmI GAANNNNTTC 2 cut(s) 1167, 3162
PflMI CCANNNNNTGG 1 cut(s) 1778
PfoI TCCNGGA 1 cut(s) 193
PleI GAGTC 5 cut(s) 280, 973, 2738, 3290, 3568
PpsI GAGTC 5 cut(s) 280, 973, 2738, 3290, 3568
Ppu21I YACGTR 1 cut(s) 1693
PscI ACATGT 1 cut(s) 631
Psp124BI GAGCTC 1 cut(s) 3582
Psp6I CCWGG 5 cut(s) 901, 1371, 2164, 2872, 3079
PspFI CCCAGC 2 cut(s) 1635, 1771
PspGI CCWGG 5 cut(s) 901, 1371, 2164, 2872, 3079
PspN4I GGNNCC 9 cut(s) 222, 735, 756, 1584, 1769, 1770, 1783, 2869, 2870
PspOMI GGGCCC 2 cut(s) 1768, 2867
PstI CTGCAG 3 cut(s) 877, 1357, 3445
PstNI CAGNNNCTG 7 cut(s) 323, 908, 1361, 1571, 1778, 2918, 3563
PsuI RGATCY 2 cut(s) 23, 799
PteI GCGCGC 1 cut(s) 2416
PvuII CAGCTG 3 cut(s) 320, 651, 809
RsaI GTAC 8 cut(s) 169, 523, 748, 943, 1226, 1989, 2600, 3171
RsaNI GTAC 8 cut(s) 168, 522, 747, 942, 1225, 1988, 2599, 3170
RseI CAYNNNNRTG 7 cut(s) 415, 538, 1139, 2166, 2321, 2942, 2962
SacI GAGCTC 1 cut(s) 3582
SapI GCTCTTC 2 cut(s) 1609, 2214
SbfI CCTGCAGG 1 cut(s) 1357
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 5 cut(s) 280, 973, 2739, 3291, 3569
ScrFI CCNGG 7 cut(s) 195, 232, 903, 1373, 2166, 2874, 3081
SdaI CCTGCAGG 1 cut(s) 1357
SduI GDGCHC 8 cut(s) 631, 660, 1511, 1772, 1786, 2755, 2871, 3582
SfcI CTRYAG 6 cut(s) 873, 1353, 1536, 2910, 3441, 3447
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 4 cut(s) 686, 734, 1349, 2476
SmiMI CAYNNNNRTG 7 cut(s) 415, 538, 1139, 2166, 2321, 2942, 2962
SmlI CTYRAG 3 cut(s) 1478, 2387, 2657
SmoI CTYRAG 3 cut(s) 1478, 2387, 2657
Sse8387I CCTGCAGG 1 cut(s) 1357
SspI AATATT 2 cut(s) 1338, 1403
SspMI CTAG 6 cut(s) 1577, 1737, 3008, 3012, 3027, 3618
SstI GAGCTC 1 cut(s) 3582
StyD4I CCNGG 7 cut(s) 193, 230, 901, 1371, 2164, 2872, 3079
StyI CCWWGG 4 cut(s) 156, 413, 1512, 2271
TaaI ACNGT 2 cut(s) 435, 1324
TaiI ACGT 3 cut(s) 1130, 1695, 2738
TaqI TCGA 7 cut(s) 117, 488, 966, 1112, 1333, 1629, 2948
TatI WGTACW 3 cut(s) 167, 521, 1987
TauI GCSGC 5 cut(s) 185, 188, 212, 230, 295
TscAI CASTG 4 cut(s) 618, 1327, 3490, 3553
TseFI GTSAC 6 cut(s) 241, 283, 429, 1706, 2652, 2731
Tsp45I GTSAC 6 cut(s) 241, 283, 429, 1706, 2652, 2731
TspGWI ACGGA 1 cut(s) 2616
TspRI CASTG 4 cut(s) 618, 1327, 3490, 3553
Van91I CCANNNNNTGG 1 cut(s) 1778
Vha464I CTTAAG 1 cut(s) 2657
VpaK11BI GGWCC 4 cut(s) 686, 734, 1349, 2476
XagI CCTNNNNNAGG 2 cut(s) 2847, 2877
XapI RAATTY 7 cut(s) 151, 385, 472, 893, 2681, 3116, 3244
XbaI TCTAGA 1 cut(s) 3617
XceI RCATGY 6 cut(s) 537, 635, 1138, 1298, 3061, 3343
XcmI CCANNNNNNNNNTGG 1 cut(s) 2603
XmiI GTMKAC 1 cut(s) 1304
XmnI GAANNNNTTC 2 cut(s) 1167, 3162
XspI CTAG 6 cut(s) 1577, 1737, 3008, 3012, 3027, 3618
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.