RchiOBHm_Chr7g0190401

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004286
Physical Location & Seq
Forward (+)
9480635 .. 9490409
9775 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17005

Sequence Viewer

Length: 3648 bp
ATGGTTTCAACGAGGAGAAGTGGATCTCTCTCCGGCAATAACAGTAAAAGATCGTCGTCTTCCGACGACAACAAGCCTCAGTCGCCGAAGCGGCAGAAGGTGGATAACAATGGCGTTGGTTCGGAGAAAACGGAGAATTCGAAGGAGGTTTGTACGCCGGCCACGGCGGCGGCTGATCCCGGAGAATGCAGCGCCGCCGGCGAGGCTCAGACGGCCGGAGACGGCGCGACTTCTGTAAAGACTGAAGCTTCTGCACAAGCCGTGGCGGTGACCCCGCCGCCTGTGGCTGAAGGTGCTGAAGGGTCTTCGCCGGCTGCTGTGCTGGATAAGGCGAGGAGTTCCTTTTTGGCATGGAGTTCATATCAGAAGCAGAGCCTGAATTCGGGGAGTTTCGAGTTGACAACACCTTGGTGCAAGCTTCTGTCACAGTCTGGACAAAATCTAAACATTGCTATCACCACATCGATTTTTACAATTGGTTCGAGTAGAAGCAGCAATTTCCCTCTGAAGGATAATACCATTAGTGCGTGTCTGTGCAAGATCAAACGCGAACAGCGGGAGAGCGGTGCTGTAACCATTATAGAAAGTATGGGAAACAAGGGATCACTGCAAGTAAATGGGGCGCATGTGAAGAAAGGCAACAGCTGCGTGCTCAACTCAGGTGATGAAGTGGTATTTGGTTCTCTGGGGAACCATGCTTATATTTTTCAACTACTTGTGGCGGAGGGTGCGGTCAAGGGTGCAGAGGTTCAAAGTGGTGTTGGAAAATATCTGCACCTTGACAGGAGAGCCGGAGATCCTTCAGATGTGGATGGGGCTTCCATACTGGCTTCTCTTAGCATGAGACCAGAACTATCACGATGGAAGTCTGCGGCTCAGACCACCAATAAAGTCCATCCAGGGGCTGAAGTACCAGCTCAGTCTGTTATTCGAGAAGGTACAGAAGTTGAGCTTGAAGAACTCGAAGGCAGCTTGACCCCAAACAGAACAACTGACAAAACAGAAGAGATCGGAGGAATCAACAAGAGTCTTACTCCGGACTGCAACCCAGATTCGGGTGTAGAGGCAGACAATGTATTGGAAGAAAAAAATGAGTGGACGAGGGATTCGCAGCCAGCATCAACGTCAGGCATGTCTGTGCGGTGTGCTGTTTTTAAAGAAGGCATTCATGCAGCAATTCTTGAAGGCAATAGCATGGATGTTTCATTTGATGATTTCCCGTACTATTTGAGTGAGAATACGAAGAATGTGCTGATTGCAGCTTCATTTATACACTTGAAACATAAAGAACATGCAAAGTATACTACAGAGCTTACCACTGTGAATCCTCGAATATTGCTCTCTGGTCCTGCAGGGTCTGAGATATACCAGGAGATGTTGGCAAAGGCACTTGCTCAATATTTCGGGGCTAAGTTGCTCATATTTGATAGCCAATCACTTTTGGGTGGTTTGTCATCAAAGGAAGCCGAGTTGCCCAAGGATGGATCTAATGGAGATAAAATAAGCACCTTGGCCAAACCAAGTCCTGTTTCCACAGACTTGTCTAAGAGCATTGATCCATCAGTTTCTGAGCTAGAGGCACCAAGGTTGGCGCCAAGCTCTTCAAATGGACCTTCATATGGCCTGGAATCCCAACCTAAGACGGAGATTGACTCCATCGCTACATCCTCAGGGACATCTAAGAATTACGTGTTTAAAATAGGTGACAGAGTAAAGTTTATTGGTTTATCTTCGGGCAACTTATATCCTACAACATCTTCACGGGGCCCAGTACCTGGAGCCCGAGGAGAGGTTGCGTTGGTTTTTGAGGACAATCCTTTGTCAAAAATTGGTGTGAGGTTTGATAAACAAGTATTTGATGGTGTTGATCTTGGGGGTCTTTGTAAAGGAAATGGATTCTTCTGCAATGCCTCCGATCTTCGTTCAGAAAACACAGGTGCGGATGATCTGGATAAATTACTGATCAATACCTTGTTTGAGGCTGTACATAGTGAGAGCAGAGGTTCTCCTTTCATTTTGTTCATGAAAGATGCGGAGAAGTCCCTTGTAGGAAATTCAGATTCATATTCCACATTCAGAGGCAGACTTGATAAGCTGCCTGATAACGTGGTTGTAATTGGCTCTCATACTCATACTGACAATCGCAAGGAGAAGTCGCATCCTGGTGGTCTACTTTTCACAAAGTTCGGTAGCAATCAAACTGCTCTTCTTGATTTGGCTTTTCCGGATAGTTTTGGAAGACTGCATGAAAGAGGGAAGGAAGTCCCCAAGGCAACAAAACTTCTGACGAAACTTTTTCCTAATAAAGTAACCATTCATATGCCACAGGATGAGGGACTTCTTGTCTCATGGAAACAGCAATTGGATCGTGATGCCGAAACCCTCAAAATGAAGGGAAATTTGAACCACTTGCGCACTGTTCTGGGTCGGTGTGGGATGGAATGTGAAGGACTTGATACGCTAAGCATCAAGGACCAAACACTTACAAATGAAAGTTCAGAGAAGGTAGTAGGATGGGCATTAAGCCATCATTTAATGGAGAATCCTGAAGCTGATCCAGAAACTAGAGTTGTTTTGTCTGCTGAGAGCATCAAGTACGGACTTGGAATCTTACAGTCTATCCAGAATGAAAATAAGAGCTTGAAGAAGTCACTTAAGGATGTTGTCACGGAAAATGAATTTGAGAAAAGGCTTTTGGCTGATGTTATCCCACCTACTGACATTGGAGTTACATTTGATGATATTGGCGCTCTTGAAAATGTGAAGGATACATTGAAAGAATTGGTGATGCTGCCTTTACAAAGGCCGGAGCTTTTCTGCAAAGGGCAATTAACCAAGCCTTGCAAAGGGATCCTTTTATTTGGTCCCCCGGGGACAGGCAAGACAATGCTGGCAAAGGCTGTGGCTACAGAAGCTGGTGCAAACTTCATCAACATATCCATGTCGAGCATCACATCTAAGTGGTTTGGTGAGGGTGAGAAGTATGTGAAAGCTGTTTTCTCTCTAGCTAGTAAAATTGCCCCTAGTGTCGTATTTGTTGATGAAGTTGACAGCATGTTGGGCCGGCGGGAAAATCCAGGGGAGCATGAGGCAATGCGCAAGATGAAAAATGAATTTATGGTAAATTGGGATGGGCTACGAACAAAAGAAGCAGAACGAGTTCTGGTACTTGCAGCCACAAATAGGCCTTTTGACCTTGATGAAGCTGTCATTCGAAGGCTGCCCCGAAGGTTGATGGTAAATTTGCCAGATGCTCCCAATAGGGCAAAGATATTAAGAGTCATATTGGCAAAAGAGGACTTATCTCCCGATGTTGATTTCGAGGCAATTGCGAGCATGTCAGATGGATATTCTGGAAGTGACCTTAAAAATCTATGCGTGGCTGCTGCACACCGTCCAATTAAAGAGATTTTGGAAAAGGAAAAGAAGGATCGTGCTGCAGCTATAGCAGAAGGTAAACCTATTCCAGCATTGAGTAGCAGTGGCGATGTTCGGCCTGTGAACATGGATGATTTTAAATATGCTCATGAACAGGTATGTGCCAGTGTATCATCGGAGTCTGTCAACATGACAGAGCTCTTACAATGGAATGAACTATATGGAGAAGGGGGTTCTAGAAGAAAGAAGGCCCTTAGTTACTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

1215

Amino Acids

131.19

Weight (kDa)

5.8

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 158 - 227 4.3e-06 FHA domain
DUF7751 PF24933 813 - 872 1.4e-18 Domain of unknown function (DUF7751)
AAA PF00004 951 - 1081 1.4e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 1104 - 1140 3.8e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 2414, 3098
AccB1I GGYRCC 2 cut(s) 1579, 1591
AccB7I CCANNNNNTGG 1 cut(s) 1775
AccBSI CCGCTC 1 cut(s) 564
AccI GTMKAC 2 cut(s) 1301, 2170
AccII CGCG 2 cut(s) 227, 549
AccIII TCCGGA 2 cut(s) 1036, 2224
AcoI YGGCCR 3 cut(s) 159, 213, 1512
AcsI RAATTY 7 cut(s) 136, 379, 2053, 2398, 2678, 3113, 3241
AcuI CTGAAG 7 cut(s) 264, 309, 318, 527, 786, 927, 2568
AcyI GRCGYC 1 cut(s) 1592
AfaI GTAC 8 cut(s) 154, 912, 940, 1223, 1773, 1986, 2597, 3168
AflII CTTAAG 1 cut(s) 2654
AflIII ACRYGT 1 cut(s) 1689
AhdI GACNNNNNGTC 1 cut(s) 2342
AjnI CCWGG 6 cut(s) 898, 1368, 1623, 1774, 2161, 3076
AjuI GAANNNNNNNTTGG 8 cut(s) 660, 692, 1513, 1545, 2345, 2377, 2678, 2710
AleI CACNNNNGTG 1 cut(s) 409
Alw21I GWGCWC 2 cut(s) 654, 3579
Alw26I GTCTC 3 cut(s) 213, 838, 2350
AlwNI CAGNNNCTG 6 cut(s) 376, 905, 1358, 1568, 1775, 2915
Ama87I CYCGRG 2 cut(s) 1782, 2869
Aor13HI TCCGGA 2 cut(s) 1036, 2224
ApaI GGGCCC 1 cut(s) 1769
ApoI RAATTY 7 cut(s) 136, 379, 2053, 2398, 2678, 3113, 3241
ArsI GACNNNNNNTTYG 2 cut(s) 1090, 1122
Asp700I GAANNNNTTC 2 cut(s) 1164, 3159
AspLEI GCGC 7 cut(s) 194, 227, 625, 1594, 2415, 2750, 3099
AspS9I GGNCC 8 cut(s) 1346, 1610, 1765, 1766, 2473, 2864, 3061, 3628
AsuC2I CCSGG 3 cut(s) 180, 2870, 2871
AsuHPI GGTGA 7 cut(s) 280, 448, 674, 1715, 2797, 2981, 2987
AsuII TTCGAA 2 cut(s) 140, 3214
AvaI CYCGRG 2 cut(s) 1782, 2869
AvaII GGWCC 4 cut(s) 1346, 1610, 2473, 2864
AxyI CCTNAGG 1 cut(s) 1669
BaeGI GKGCMC 1 cut(s) 1769
BaeI ACNNNNGTAYC 2 cut(s) 930, 963
BalI TGGCCA 1 cut(s) 1514
BamHI GGATCC 1 cut(s) 2850
BanI GGYRCC 2 cut(s) 1579, 1591
BanII GRGCYC 3 cut(s) 1769, 1783, 3579
BbsI GAAGAC 3 cut(s) 51, 297, 2245
Bbv12I GWGCWC 2 cut(s) 654, 3579
BceAI ACGGC 4 cut(s) 180, 228, 238, 245
BcgI CGANNNNNNTGC 6 cut(s) 1231, 1265, 2348, 2382, 3311, 3345
BciT130I CCWGG 6 cut(s) 900, 1370, 1625, 1776, 2163, 3078
BciVI GTATCC 1 cut(s) 2761
BclI TGATCA 1 cut(s) 1962
BcnI CCSGG 3 cut(s) 180, 2870, 2871
BcoDI GTCTC 3 cut(s) 213, 838, 2350
BfaI CTAG 6 cut(s) 1574, 2565, 3005, 3009, 3024, 3615
BfmI CTRYAG 5 cut(s) 1305, 1350, 2907, 3438, 3444
BfoI RGCGCY 3 cut(s) 195, 1595, 2751
BfrI CTTAAG 1 cut(s) 2654
BfuI GTATCC 1 cut(s) 2761
BglI GCCNNNNNGGC 3 cut(s) 91, 167, 203
BlpI GCTNAGC 1 cut(s) 2462
Bme1390I CCNGG 9 cut(s) 180, 900, 1370, 1625, 1776, 2163, 2870, 2871, 3078
Bme18I GGWCC 4 cut(s) 1346, 1610, 2473, 2864
BmeRI GACNNNNNGTC 1 cut(s) 2342
BmeT110I CYCGRG 2 cut(s) 1782, 2869
BmgT120I GGNCC 8 cut(s) 1346, 1610, 1765, 1766, 2473, 2864, 3061, 3628
BmiI GGNNCC 8 cut(s) 692, 1581, 1593, 1766, 1767, 1780, 2852, 2866
BmrFI CCNGG 9 cut(s) 180, 900, 1370, 1625, 1776, 2163, 2870, 2871, 3078
BmrI ACTGGG 1 cut(s) 1763
BmsI GCATC 9 cut(s) 1127, 2020, 2167, 2362, 2475, 2598, 2778, 2958, 3241
BmuI ACTGGG 1 cut(s) 1763
BpiI GAAGAC 3 cut(s) 51, 297, 2245
BplI GAGNNNNNCTC 4 cut(s) 1018, 1050, 1637, 1669
BpmI CTGGAG 1 cut(s) 1797
Bpu1102I GCTNAGC 1 cut(s) 2462
Bpu14I TTCGAA 2 cut(s) 140, 3214
BpuMI CCSGG 3 cut(s) 180, 2870, 2871
Bsa29I ATCGAT 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 1690
BsaHI GRCGYC 1 cut(s) 1592
BsaI GGTCTC 1 cut(s) 838
BsaWI WCCGGW 2 cut(s) 1036, 2224
Bse118I RCCGGY 4 cut(s) 157, 197, 310, 3063
Bse1I ACTGG 3 cut(s) 831, 1769, 3543
Bse21I CCTNAGG 1 cut(s) 1669
Bse3DI GCAATG 3 cut(s) 447, 1912, 3099
BseAI TCCGGA 2 cut(s) 1036, 2224
BseBI CCWGG 6 cut(s) 900, 1370, 1625, 1776, 2163, 3078
BseCI ATCGAT 1 cut(s) 464
BseMI GCAATG 3 cut(s) 447, 1912, 3099
BseMII CTCAG 9 cut(s) 92, 221, 672, 890, 932, 1350, 1560, 1683, 2574
BseNI ACTGG 3 cut(s) 831, 1769, 3543
BseRI GAGGAG 3 cut(s) 28, 349, 1800
BseSI GKGCMC 1 cut(s) 1769
BseX3I CGGCCG 1 cut(s) 213
BsgI GTGCAG 4 cut(s) 237, 758, 762, 3372
Bsh1236I CGCG 2 cut(s) 227, 549
Bsh1285I CGRYCG 1 cut(s) 216
BshNI GGYRCC 2 cut(s) 1579, 1591
BshVI ATCGAT 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 654, 3579
BsiHKCI CYCGRG 2 cut(s) 1782, 2869
BslFI GGGAC 6 cut(s) 1687, 2026, 2249, 2349, 2850, 2887
BsmAI GTCTC 3 cut(s) 213, 838, 2350
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 6 cut(s) 1687, 2026, 2249, 2349, 2850, 2887
BsmI GAATGC 2 cut(s) 191, 1164
Bso31I GGTCTC 1 cut(s) 838
BsoBI CYCGRG 2 cut(s) 1782, 2869
Bsp119I TTCGAA 2 cut(s) 140, 3214
Bsp120I GGGCCC 1 cut(s) 1765
Bsp1286I GDGCHC 4 cut(s) 654, 1769, 1783, 3579
Bsp13I TCCGGA 2 cut(s) 1036, 2224
Bsp1407I TGTACA 1 cut(s) 1984
Bsp1720I GCTNAGC 1 cut(s) 2462
BspCNI CTCAG 9 cut(s) 91, 220, 671, 889, 931, 1351, 1561, 1682, 2575
BspDI ATCGAT 1 cut(s) 464
BspEI TCCGGA 2 cut(s) 1036, 2224
BspFNI CGCG 2 cut(s) 227, 549
BspHI TCATGA 2 cut(s) 2022, 3526
BspLI GGNNCC 8 cut(s) 692, 1581, 1593, 1766, 1767, 1780, 2852, 2866
BspMAI CTGCAG 2 cut(s) 1354, 3442
BspQI GCTCTTC 2 cut(s) 1606, 2211
BspT104I TTCGAA 2 cut(s) 140, 3214
BspT107I GGYRCC 2 cut(s) 1579, 1591
BspTI CTTAAG 1 cut(s) 2654
BspTNI GGTCTC 1 cut(s) 838
BsrBI CCGCTC 1 cut(s) 564
BsrDI GCAATG 3 cut(s) 447, 1912, 3099
BsrFI RCCGGY 4 cut(s) 157, 197, 310, 3063
BsrGI TGTACA 1 cut(s) 1984
BsrI ACTGG 3 cut(s) 831, 1769, 3543
BssAI RCCGGY 4 cut(s) 157, 197, 310, 3063
BssNAI GTATAC 1 cut(s) 1302
BssNI GRCGYC 1 cut(s) 1592
BssT1I CCWWGG 5 cut(s) 407, 1476, 1509, 1583, 2268
Bst1107I GTATAC 1 cut(s) 1302
Bst2UI CCWGG 6 cut(s) 900, 1370, 1625, 1776, 2163, 3078
Bst4CI ACNGT 6 cut(s) 44, 429, 1321, 2419, 2616, 3395
Bst6I CTCTTC 3 cut(s) 999, 1606, 2211
BstACI GRCGYC 1 cut(s) 1592
BstAFI CTTAAG 1 cut(s) 2654
BstAPI GCANNNNNTGC 1 cut(s) 645
BstAUI TGTACA 1 cut(s) 1984
BstBAI YACGTR 1 cut(s) 1690
BstBI TTCGAA 2 cut(s) 140, 3214
BstC8I GCNNGC 9 cut(s) 159, 199, 312, 416, 650, 1116, 2892, 3065, 3334
BstDSI CCRYGG 2 cut(s) 162, 261
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 2844
BstFNI CGCG 2 cut(s) 227, 549
BstH2I RGCGCY 3 cut(s) 195, 1595, 2751
BstHHI GCGC 7 cut(s) 194, 227, 625, 1594, 2415, 2750, 3099
BstMAI GTCTC 3 cut(s) 213, 838, 2350
BstMCI CGRYCG 1 cut(s) 216
BstNI CCWGG 6 cut(s) 900, 1370, 1625, 1776, 2163, 3078
BstNSI RCATGY 5 cut(s) 629, 1135, 1295, 3058, 3340
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 9 cut(s) 178, 898, 1368, 1623, 1774, 2161, 2868, 2869, 3076
BstSFI CTRYAG 5 cut(s) 1305, 1350, 2907, 3438, 3444
BstSLI GKGCMC 1 cut(s) 1769
BstUI CGCG 2 cut(s) 227, 549
BstV2I GAAGAC 3 cut(s) 51, 297, 2245
BstX2I RGATCY 4 cut(s) 23, 796, 1484, 2850
BstYI RGATCY 4 cut(s) 23, 796, 1484, 2850
BstZ17I GTATAC 1 cut(s) 1302
BstZI CGGCCG 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 464
Bsu36I CCTNAGG 1 cut(s) 1669
BsuI GTATCC 1 cut(s) 2761
BsuTUI ATCGAT 1 cut(s) 464
BtgI CCRYGG 2 cut(s) 162, 261
BtgZI GCGATG 2 cut(s) 1642, 3501
BtsI GCAGTG 2 cut(s) 605, 3487
BtsIMutI CAGTG 5 cut(s) 605, 1317, 2415, 3487, 3550
Cac8I GCNNGC 9 cut(s) 159, 199, 312, 416, 650, 1116, 2892, 3065, 3334
CaiI CAGNNNCTG 6 cut(s) 376, 905, 1358, 1568, 1775, 2915
CciI TCATGA 2 cut(s) 2022, 3526
CfoI GCGC 7 cut(s) 194, 227, 625, 1594, 2415, 2750, 3099
Cfr10I RCCGGY 4 cut(s) 157, 197, 310, 3063
Cfr13I GGNCC 8 cut(s) 1346, 1610, 1765, 1766, 2473, 2864, 3061, 3628
Cfr9I CCCGGG 1 cut(s) 2869
ClaI ATCGAT 1 cut(s) 464
Csp6I GTAC 8 cut(s) 153, 911, 939, 1222, 1772, 1985, 2596, 3167
CspCI CAANNNNNGTGG 2 cut(s) 2883, 2918
CviQI GTAC 8 cut(s) 153, 911, 939, 1222, 1772, 1985, 2596, 3167
DinI GGCGCC 1 cut(s) 1593
DraI TTTAAA 3 cut(s) 1156, 1696, 3517
DriI GACNNNNNGTC 1 cut(s) 2342
EaeI YGGCCR 3 cut(s) 159, 213, 1512
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 3 cut(s) 999, 1606, 2211
Eam1105I GACNNNNNGTC 1 cut(s) 2342
EarI CTCTTC 3 cut(s) 999, 1606, 2211
EciI GGCGGA 1 cut(s) 737
Ecl136II GAGCTC 1 cut(s) 3577
EclXI CGGCCG 1 cut(s) 213
Eco130I CCWWGG 5 cut(s) 407, 1476, 1509, 1583, 2268
Eco147I AGGCCT 1 cut(s) 3187
Eco24I GRGCYC 3 cut(s) 1769, 1783, 3579
Eco31I GGTCTC 1 cut(s) 838
Eco47I GGWCC 4 cut(s) 1346, 1610, 2473, 2864
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 3577
Eco57I CTGAAG 7 cut(s) 264, 309, 318, 527, 786, 927, 2568
Eco81I CCTNAGG 1 cut(s) 1669
Eco88I CYCGRG 2 cut(s) 1782, 2869
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 3577
EcoNI CCTNNNNNAGG 1 cut(s) 2844
EcoO109I RGGNCCY 2 cut(s) 1765, 3628
EcoO65I GGTNACC 1 cut(s) 268
EcoRI GAATTC 2 cut(s) 136, 379
EcoRII CCWGG 6 cut(s) 898, 1368, 1623, 1774, 2161, 3076
EcoT14I CCWWGG 5 cut(s) 407, 1476, 1509, 1583, 2268
EcoT38I GRGCYC 3 cut(s) 1769, 1783, 3579
EgeI GGCGCC 1 cut(s) 1593
EheI GGCGCC 1 cut(s) 1593
ErhI CCWWGG 5 cut(s) 407, 1476, 1509, 1583, 2268
Esp3I CGTCTC 1 cut(s) 213
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 2838, 2870
FaqI GGGAC 6 cut(s) 1687, 2026, 2249, 2349, 2850, 2887
FauI CCCGC 3 cut(s) 282, 549, 3060
FauNDI CATATG 2 cut(s) 1618, 2319
FbaI TGATCA 1 cut(s) 1962
FblI GTMKAC 2 cut(s) 1301, 2170
FriOI GRGCYC 3 cut(s) 1769, 1783, 3579
FspBI CTAG 6 cut(s) 1574, 2565, 3005, 3009, 3024, 3615
FspI TGCGCA 2 cut(s) 2414, 3098
GlaI GCGC 7 cut(s) 193, 226, 624, 1593, 2414, 2749, 3098
GsuI CTGGAG 1 cut(s) 1797
HaeII RGCGCY 3 cut(s) 195, 1595, 2751
HhaI GCGC 7 cut(s) 194, 227, 625, 1594, 2415, 2750, 3099
Hin1I GRCGYC 1 cut(s) 1592
Hin6I GCGC 7 cut(s) 192, 225, 623, 1592, 2413, 2748, 3097
HinP1I GCGC 7 cut(s) 192, 225, 623, 1592, 2413, 2748, 3097
HincII GTYRAC 3 cut(s) 399, 3049, 3565
HindII GTYRAC 3 cut(s) 399, 3049, 3565
HindIII AAGCTT 2 cut(s) 246, 416
HphI GGTGA 7 cut(s) 280, 448, 674, 1715, 2797, 2981, 2987
Hpy166II GTNNAC 8 cut(s) 399, 1098, 1302, 2171, 3049, 3458, 3502, 3565
Hpy8I GTNNAC 8 cut(s) 399, 1098, 1302, 2171, 3049, 3458, 3502, 3565
Hpy99I CGWCG 2 cut(s) 58, 68
HpyCH4III ACNGT 6 cut(s) 44, 429, 1321, 2419, 2616, 3395
HpyCH4IV ACGT 3 cut(s) 1124, 1689, 2106
HpySE526I ACGT 3 cut(s) 1124, 1689, 2106
Hsp92I GRCGYC 1 cut(s) 1592
HspAI GCGC 7 cut(s) 192, 225, 623, 1592, 2413, 2748, 3097
KasI GGCGCC 1 cut(s) 1591
Kpn2I TCCGGA 2 cut(s) 1036, 2224
KroI GCCGGC 4 cut(s) 157, 197, 310, 3063
KroNI GCCGGC 4 cut(s) 159, 199, 312, 3065
Ksp22I TGATCA 1 cut(s) 1962
LguI GCTCTTC 2 cut(s) 1606, 2211
LmnI GCTCC 4 cut(s) 1778, 2809, 3082, 3259
LweI GCATC 9 cut(s) 1127, 2020, 2167, 2362, 2475, 2598, 2778, 2958, 3241
MaeI CTAG 6 cut(s) 1574, 2565, 3005, 3009, 3024, 3615
MaeII ACGT 3 cut(s) 1124, 1689, 2106
MbiI CCGCTC 1 cut(s) 564
MfeI CAATTG 3 cut(s) 474, 2360, 3327
MflI RGATCY 4 cut(s) 23, 796, 1484, 2850
MhlI GDGCHC 4 cut(s) 654, 1769, 1783, 3579
MlsI TGGCCA 1 cut(s) 1514
MluNI TGGCCA 1 cut(s) 1514
Mly113I GGCGCC 1 cut(s) 1592
MlyI GAGTC 4 cut(s) 1036, 1646, 3288, 3566
MmeI TCCRAC 2 cut(s) 87, 742
Mox20I TGGCCA 1 cut(s) 1514
MreI CGCCGGCG 1 cut(s) 197
MroI TCCGGA 2 cut(s) 1036, 2224
MroNI GCCGGC 4 cut(s) 157, 197, 310, 3063
MroXI GAANNNNTTC 2 cut(s) 1164, 3159
MscI TGGCCA 1 cut(s) 1514
MslI CAYNNNNRTG 6 cut(s) 409, 1136, 2163, 2318, 2939, 2959
Msp20I TGGCCA 1 cut(s) 1514
MspA1I CMGCKG 2 cut(s) 556, 645
MspCI CTTAAG 1 cut(s) 2654
MspR9I CCNGG 9 cut(s) 180, 900, 1370, 1625, 1776, 2163, 2870, 2871, 3078
MunI CAATTG 3 cut(s) 474, 2360, 3327
Mva1269I GAATGC 2 cut(s) 191, 1164
MvaI CCWGG 6 cut(s) 900, 1370, 1625, 1776, 2163, 3078
MvnI CGCG 2 cut(s) 227, 549
NaeI GCCGGC 4 cut(s) 159, 199, 312, 3065
NarI GGCGCC 1 cut(s) 1592
NciI CCSGG 3 cut(s) 180, 2870, 2871
NdeI CATATG 2 cut(s) 1618, 2319
NgoMIV GCCGGC 4 cut(s) 157, 197, 310, 3063
NlaIV GGNNCC 8 cut(s) 692, 1581, 1593, 1766, 1767, 1780, 2852, 2866
NmeAIII GCCGAG 1 cut(s) 1492
NmuCI GTSAC 6 cut(s) 268, 423, 1703, 2649, 2665, 3359
NsbI TGCGCA 2 cut(s) 2414, 3098
NspI RCATGY 5 cut(s) 629, 1135, 1295, 3058, 3340
NspV TTCGAA 2 cut(s) 140, 3214
OliI CACNNNNGTG 1 cut(s) 409
PagI TCATGA 2 cut(s) 2022, 3526
PceI AGGCCT 1 cut(s) 3187
PciSI GCTCTTC 2 cut(s) 1606, 2211
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 2 cut(s) 191, 1164
PdiI GCCGGC 4 cut(s) 159, 199, 312, 3065
PdmI GAANNNNTTC 2 cut(s) 1164, 3159
PfeI GAWTC 9 cut(s) 1017, 1052, 1106, 1324, 1628, 1896, 2060, 2542, 2607
PflMI CCANNNNNTGG 1 cut(s) 1775
PfoI TCCNGGA 1 cut(s) 178
PleI GAGTC 4 cut(s) 1035, 1646, 3287, 3565
PluTI GGCGCC 1 cut(s) 1595
PpsI GAGTC 4 cut(s) 1035, 1646, 3287, 3565
Ppu21I YACGTR 1 cut(s) 1690
Psp124BI GAGCTC 1 cut(s) 3579
Psp6I CCWGG 6 cut(s) 898, 1368, 1623, 1774, 2161, 3076
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 6 cut(s) 898, 1368, 1623, 1774, 2161, 3076
PspN4I GGNNCC 8 cut(s) 692, 1581, 1593, 1766, 1767, 1780, 2852, 2866
PspOMI GGGCCC 1 cut(s) 1765
PspPI GGNCC 8 cut(s) 1346, 1610, 1765, 1766, 2473, 2864, 3061, 3628
PstI CTGCAG 2 cut(s) 1354, 3442
PstNI CAGNNNCTG 6 cut(s) 376, 905, 1358, 1568, 1775, 2915
PsuI RGATCY 4 cut(s) 23, 796, 1484, 2850
PvuII CAGCTG 1 cut(s) 645
RsaI GTAC 8 cut(s) 154, 912, 940, 1223, 1773, 1986, 2597, 3168
RsaNI GTAC 8 cut(s) 153, 911, 939, 1222, 1772, 1985, 2596, 3167
RseI CAYNNNNRTG 6 cut(s) 409, 1136, 2163, 2318, 2939, 2959
SacI GAGCTC 1 cut(s) 3579
SapI GCTCTTC 2 cut(s) 1606, 2211
Sau96I GGNCC 8 cut(s) 1346, 1610, 1765, 1766, 2473, 2864, 3061, 3628
SbfI CCTGCAGG 1 cut(s) 1354
SchI GAGTC 4 cut(s) 1036, 1646, 3288, 3566
ScrFI CCNGG 9 cut(s) 180, 900, 1370, 1625, 1776, 2163, 2870, 2871, 3078
SdaI CCTGCAGG 1 cut(s) 1354
SduI GDGCHC 4 cut(s) 654, 1769, 1783, 3579
SfaNI GCATC 9 cut(s) 1127, 2020, 2167, 2362, 2475, 2598, 2778, 2958, 3241
SfcI CTRYAG 5 cut(s) 1305, 1350, 2907, 3438, 3444
SfoI GGCGCC 1 cut(s) 1593
SfuI TTCGAA 2 cut(s) 140, 3214
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 4 cut(s) 1346, 1610, 2473, 2864
SmaI CCCGGG 1 cut(s) 2871
SmiMI CAYNNNNRTG 6 cut(s) 409, 1136, 2163, 2318, 2939, 2959
SmlI CTYRAG 1 cut(s) 2654
SmoI CTYRAG 1 cut(s) 2654
Sse8387I CCTGCAGG 1 cut(s) 1354
SseBI AGGCCT 1 cut(s) 3187
SspDI GGCGCC 1 cut(s) 1591
SspI AATATT 2 cut(s) 1335, 1400
SspMI CTAG 6 cut(s) 1574, 2565, 3005, 3009, 3024, 3615
SstI GAGCTC 1 cut(s) 3579
StuI AGGCCT 1 cut(s) 3187
StyD4I CCNGG 9 cut(s) 178, 898, 1368, 1623, 1774, 2161, 2868, 2869, 3076
StyI CCWWGG 5 cut(s) 407, 1476, 1509, 1583, 2268
TaaI ACNGT 6 cut(s) 44, 429, 1321, 2419, 2616, 3395
TaiI ACGT 3 cut(s) 1127, 1692, 2109
TatI WGTACW 1 cut(s) 1984
TauI GCSGC 6 cut(s) 94, 170, 173, 197, 280, 875
TfiI GAWTC 9 cut(s) 1017, 1052, 1106, 1324, 1628, 1896, 2060, 2542, 2607
TscAI CASTG 5 cut(s) 612, 1324, 2422, 3487, 3550
TseFI GTSAC 6 cut(s) 268, 423, 1703, 2649, 2665, 3359
Tsp45I GTSAC 6 cut(s) 268, 423, 1703, 2649, 2665, 3359
TspGWI ACGGA 4 cut(s) 146, 1658, 2613, 2684
TspMI CCCGGG 1 cut(s) 2869
TspRI CASTG 5 cut(s) 612, 1324, 2422, 3487, 3550
Van91I CCANNNNNTGG 1 cut(s) 1775
Vha464I CTTAAG 1 cut(s) 2654
VpaK11BI GGWCC 4 cut(s) 1346, 1610, 2473, 2864
XagI CCTNNNNNAGG 1 cut(s) 2844
XapI RAATTY 7 cut(s) 136, 379, 2053, 2398, 2678, 3113, 3241
XbaI TCTAGA 1 cut(s) 3614
XceI RCATGY 5 cut(s) 629, 1135, 1295, 3058, 3340
XmaI CCCGGG 1 cut(s) 2869
XmiI GTMKAC 2 cut(s) 1301, 2170
XmnI GAANNNNTTC 2 cut(s) 1164, 3159
XspI CTAG 6 cut(s) 1574, 2565, 3005, 3009, 3024, 3615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.