Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003320

Physical Location & Seq

Genomic Coordinates Forward (+)

61993778 .. 62003979

Gene Structure 10202 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0504400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGATGAATACTATTCTGACCAGAGCCTCTCTCTCGCCGGACCCGAAGCTCCTGTTCAGCTACACCACCAACTCCAATGTCGACCGCTTCGCCTTCAACTTCAAGCCTCACCTCCCGATACGCCGTCGTTTCCCTCATCGCCGTCATCTCCGGCAGCAGTCGAACCCTAATTTCACTCTCTCCAGCTGCCTGCAGGCCGCCGCGGCCCCAAATCATATCGCCACGGCGGCGGCGCCGATTCAGGGGGAGGCGTCGGTGCTGCTCGACGTCTCCGGGATGTTGTGCGGCGGCTGTGTTTCGCGAGTCAAGTCGGTGCTGTCTGCGGACGACCGAGTCCACTCGGTGGCGGTCAACATGTTGACCGAGACGGCAGCGATTAAGCTGAGGCCGGAGGTTGAAGGTGGAGCGGAGGCCGTGGCGGAGAGCTTGGCCGGAAGGTTGACGGAGTGCGGATTCGCGGCGAGGAGGAGGGCTTCCGGGATGGGAGTGACGGAGAGTGTGAGGAAGTGGAAGGAGATGGTGAAGAAGAAGGAGGAGATGCTGGTGAAGAGTAGGAACCGAGTCATTTTGGCTTGGACTCTGGTGGCCTTGTGCTGTGGATCACACGCTTCCCACATTTTGCATTCTCTGGGAATTCACATTGCTCATGGATCGTATATGGATGTGCTCCATAATTCGTATGTGAAGGGTGGTTTGGCTATGGCAGCTTTGTTGGGACCCGGACGAGACTTGCTTTTTGATGGTTTGAGGGCTTTCAAAAAAGGATCACCCAATATGAATTCTCTTGTGGGATTTGGATCCCTGGCTGCTTTTACAATTAGTGCGGTCTCTCTTCTTAACCCTGAACTTCAATGGGATGCAGCCTTCTTTGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTTGAAGAAAGGGCAAGGATCAGGGCATCTAGTGATATGAACGAACTCTTGTCACTGATAAACACTCAATCAAGACTGGTAATTGCATCCTCAGAAAATGATTCCTCCAGTGACACTGTACTTGGCTCCGATGCAATTTGCATTGAGGTCCCAACTGATGATGTTCGTGTGGGAGATTCTGTGTTGGTATTGCCAGGAGAAACTATACCCGTGGATGGAAGAGTTCTGGCTGGAAGAAGTGTTGTGGATGAGTCCATGCTCACAGGAGAATCGCTTCCTGTATTTAAGGAAAAAGAACTTACCGTCTCAGCTGGAACAATAAATTGGGATGGTCCTCTGAGGATTGAAGCAACTTCAACTGGCTCCAATTCAATGATTTCCAAGATTGTTCGCATGGTTGAGGATGCTCAAGGCCATGAAGCACCAATACAAAGGCTTGCTGATTCAATAGCTGGACCTTTTGTATACACTATAATGACTCTCTCAGCTACAACATTTGCTTTTTGGTACTACATTGGAACTCATATTTTTCCTGATGTTTTGCTCAATGATATTGCTGGGCCAGATGGAGATTCATTGCTGTTGAGCTTGAAACTAGCTGTGGATGTCTTGGTAGTTTCTTGCCCTTGTGCACTGGGTCTTGCAACACCCACAGCAATCCTAGTTGGCACTTCTCTTGGAGCAAGGCAAGGACTCCTTGTGAGAGGAGCAGATGTATTGGAACGCTTGGCCAGTATAGATCATATTGCTTTAGACAAGACAGGAACCCTCACTGAAGGAAAACCTGCTGTTTCTAGCATTGCTTCTTTCAAGTATAAAGACTCAGAAATTCTTCAAATAGCTGCTGCTGTAGAGAGTACAGCATCACATCCAATTGCTGATGCTATCTTAAACAAAGCCAAATCATTGGAGTTGAGTATCCCAGTCACAAAAAGGCAATTAACAGAACCAGGTTTTGGAACATTAGCAGAAGTAGATGGCCTTTTGGTTGCAGTGGGTTCTTTGGAATGGGTTCATGAGCGTTTCCAGAGAAGAACAGATCGATCTGACATTTCAAACCTAGAGCATGCCGTGCATCAATCATCAGAAGGCATAACACCCTCAAATTATTCAAAAACAATTGTATACGTTGGACGTGAAGGGGAAGGTATCATCGGTGCTATTGCAATATCCGACAGCCTGCGGGAAGATGCTGAGTTTACTGTAACTAGACTCCAGCAGAAGGGTATCAGAACTGTCCTGTTTTCTGGGGACAGGGAAGAGGCAGTTGCTACTATAGCAAAATCAGTTGGAATAGAAAATGAATTTATCAAGTCATCGTTGACTCCACAGGGAAAATCTGGAGCTATTTCTAGTCTTAAAGCTGCAGGGCATCATGTTGCAATGGTTGGTGATGGCATAAATGATGCGCCTTCTTTGGCTCTTGCCGACGTTGGGATTGCTCTTCAGATTGAGGGACAAGAAAATGCTGCTTCAAATGCTGCATCGATTATACTACTTGGAAACAAATTATCACAAGTTGTAGATGCACTGGAACTTGCACAGGCAACAATGGGAAAAGTGTATCAAAATTTATCTTGGGCTGTTGCATACAACGTAATTGCCATCCCCATTGCTGCTGGAGTACTGCTTCCTCAATATGATTTTGCCATGACACCATCACTTTCTGGTGGGATGATGGCCTTGAGCTCGATATTTGTAGTAACCAACTCTTTGCTTCTACAGCTTCATAAGTCTGAAAGTGCTAGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF1_GNE

Protein Analysis

890

Amino Acids

94.83

Weight (kDa)

5.94

Isoelectric Point (pI)

37.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	88 - 132	1.1e-09	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	348 - 439	1.1e-27	P-type ATPase actuator domain
Hydrolase	PF00702	551 - 782	1.5e-24	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003320)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30230 AT1G30230 AT2G18110
fragaria_vesca	FvH4_5g08071 FvH4_5g08120 FvH4_7g00650 FvH4_7g00650
malus_domestica	MD02G1316000.v1.1 MD06G1013800.v1.1 MD07G1005900.v1.1
prunus_persica	Prupe.1G190100_v2.0.a1 Prupe.2G008700_v2.0.a1 Prupe.4G287700_v2.0.a1 Prupe.4G287700_v2.0.a1
pyrus_communis	pycom02g26470
rosa_chinensis	RchiOBHm_Chr1g0314841 RchiOBHm_Chr7g0190381 RchiOBHm_Chr7g0190391
rosa_laevigata	RLG00000004537 RLG00000030760
rosa_multiflora	Rmu_co8123596.1_g000001 Rmu_ssc0000038.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264680 Rroxscaffold_4G00331840
rosa_rugosa	Rorug01G0002400 Rorug06G0504400
rosa_samantha	Rh1AG013200 Rh1BG004800 Rh1CG011700 Rh1DG006500 Rh7AG110100 Rh7BG112600 Rh7DG113100
rosa_wichuraiana	Rw1G000580 Rw1G000590 Rw7G009550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	332
AatII	GACGTC	1 cut(s)	270
Acc36I	ACCTGC	1 cut(s)	1708
AccB1I	GGYRCC	1 cut(s)	232
AccB7I	CCANNNNNTGG	2 cut(s)	343, 1871
AccBSI	CCGCTC	1 cut(s)	407
AccI	GTMKAC	3 cut(s)	81, 1380, 2040
AccII	CGCG	3 cut(s)	203, 301, 458
AclWI	GGATC	6 cut(s)	607, 658, 772, 792, 805, 941
AcoI	YGGCCR	2 cut(s)	429, 1644
AcsI	RAATTY	5 cut(s)	633, 778, 1743, 2219, 2485
AcuI	CTGAAG	2 cut(s)	1710, 2345
AcyI	GRCGYC	3 cut(s)	233, 251, 267
AdeI	CACNNNGTG	1 cut(s)	343
AfaI	GTAC	4 cut(s)	1035, 1424, 1774, 2541
AfiI	CCNNNNNNNGG	6 cut(s)	242, 343, 1130, 1871, 2137, 2349
AflIII	ACRYGT	1 cut(s)	354
AjiI	CACGTC	1 cut(s)	2051
AjnI	CCWGG	3 cut(s)	801, 1108, 1864
AjuI	GAANNNNNNNTTGG	6 cut(s)	677, 709, 1222, 1254, 1854, 1886
Alw21I	GWGCWC	4 cut(s)	669, 903, 1549, 2606
Alw26I	GTCTC	5 cut(s)	274, 359, 720, 832, 1225
Alw44I	GTGCAC	1 cut(s)	1545
AlwI	GGATC	6 cut(s)	607, 658, 772, 792, 805, 941
ApaLI	GTGCAC	1 cut(s)	1545
ApoI	RAATTY	5 cut(s)	633, 778, 1743, 2219, 2485
ArsI	GACNNNNNNTTYG	2 cut(s)	719, 751
Asp700I	GAANNNNTTC	3 cut(s)	1188, 1746, 1926
AspA2I	CCTAGG	1 cut(s)	904
AspLEI	GCGC	2 cut(s)	235, 2326
AspS9I	GGNCC	7 cut(s)	40, 205, 716, 1063, 1247, 1370, 1475
AsuC2I	CCSGG	3 cut(s)	274, 478, 720
AsuHPI	GGTGA	5 cut(s)	101, 532, 556, 759, 2318
AvaII	GGWCC	5 cut(s)	40, 716, 1063, 1247, 1370
AvrII	CCTAGG	1 cut(s)	904
BaeGI	GKGCMC	1 cut(s)	1549
BalI	TGGCCA	1 cut(s)	1646
BamHI	GGATCC	1 cut(s)	797
BanI	GGYRCC	1 cut(s)	232
BanII	GRGCYC	1 cut(s)	2606
BarI	GAAGNNNNNNTAC	2 cut(s)	1326, 1358
Bbv12I	GWGCWC	4 cut(s)	669, 903, 1549, 2606
BbvCI	CCTCAGC	1 cut(s)	383
BceAI	ACGGC	6 cut(s)	108, 126, 240, 384, 398, 1970
BciT130I	CCWGG	3 cut(s)	803, 1110, 1866
BciVI	GTATCC	1 cut(s)	1844
BcnI	CCSGG	3 cut(s)	274, 478, 720
BcoDI	GTCTC	5 cut(s)	274, 359, 720, 832, 1225
BfaI	CTAG	9 cut(s)	905, 945, 1511, 1577, 1710, 1976, 2124, 2268, 2661
BfmI	CTRYAG	5 cut(s)	191, 1764, 2190, 2280, 2636
BfoI	RGCGCY	1 cut(s)	236
BfuAI	ACCTGC	1 cut(s)	1708
BfuI	GTATCC	1 cut(s)	1844
BglI	GCCNNNNNGGC	2 cut(s)	203, 227
BlnI	CCTAGG	1 cut(s)	904
BmcAI	AGTACT	1 cut(s)	2541
Bme1390I	CCNGG	6 cut(s)	274, 478, 720, 803, 1110, 1866
Bme18I	GGWCC	5 cut(s)	40, 716, 1063, 1247, 1370
BmgBI	CACGTC	1 cut(s)	2051
BmgT120I	GGNCC	7 cut(s)	40, 205, 716, 1063, 1247, 1370, 1475
BmrFI	CCNGG	6 cut(s)	274, 478, 720, 803, 1110, 1866
BmrI	ACTGGG	2 cut(s)	1559, 1832
BmuI	ACTGGG	2 cut(s)	1559, 1832
BplI	GAGNNNNNCTC	2 cut(s)	15, 47
BpmI	CTGGAG	5 cut(s)	166, 1006, 2114, 2277, 2556
Bpu10I	CCTNAGC	1 cut(s)	383
BpuEI	CTTGAG	2 cut(s)	1308, 2620
BpuMI	CCSGG	3 cut(s)	274, 478, 720
Bsa29I	ATCGAT	2 cut(s)	1957, 2402
BsaBI	GATNNNNATC	1 cut(s)	796
BsaHI	GRCGYC	3 cut(s)	233, 251, 267
BsaI	GGTCTC	1 cut(s)	832
BsaJI	CCNNGG	6 cut(s)	201, 222, 414, 801, 904, 1125
BsaXI	ACNNNNNCTCC	4 cut(s)	1080, 1110, 1614, 1644
Bsc4I	CCNNNNNNNGG	6 cut(s)	242, 343, 1130, 1871, 2137, 2349
Bse118I	RCCGGY	1 cut(s)	877
Bse1I	ACTGG	7 cut(s)	996, 1023, 1279, 1554, 1647, 1838, 2451
Bse3DI	GCAATG	5 cut(s)	639, 1490, 1713, 2304, 2526
Bse8I	GATNNNNATC	1 cut(s)	796
BseBI	CCWGG	3 cut(s)	803, 1110, 1866
BseCI	ATCGAT	2 cut(s)	1957, 2402
BseDI	CCNNGG	6 cut(s)	201, 222, 414, 801, 904, 1125
BseJI	GATNNNNATC	1 cut(s)	796
BseLI	CCNNNNNNNGG	6 cut(s)	242, 343, 1130, 1871, 2137, 2349
BseMI	GCAATG	5 cut(s)	639, 1490, 1713, 2304, 2526
BseMII	CTCAG	7 cut(s)	374, 1020, 1236, 1244, 1413, 1752, 2100
BseNI	ACTGG	7 cut(s)	996, 1023, 1279, 1554, 1647, 1838, 2451
BseRI	GAGGAG	4 cut(s)	478, 481, 548, 1635
BseSI	GKGCMC	1 cut(s)	1549
BseYI	CCCAGC	1 cut(s)	1472
Bsh1236I	CGCG	3 cut(s)	203, 301, 458
Bsh1285I	CGRYCG	2 cut(s)	85, 331
BshNI	GGYRCC	1 cut(s)	232
BshVI	ATCGAT	2 cut(s)	1957, 2402
BsiEI	CGRYCG	2 cut(s)	85, 331
BsiHKAI	GWGCWC	4 cut(s)	669, 903, 1549, 2606
BsiSI	CCGG	8 cut(s)	38, 151, 273, 389, 432, 477, 720, 878
BslFI	GGGAC	4 cut(s)	729, 1049, 2180, 2385
BslI	CCNNNNNNNGG	6 cut(s)	242, 343, 1130, 1871, 2137, 2349
BsmAI	GTCTC	5 cut(s)	274, 359, 720, 832, 1225
BsmBI	CGTCTC	3 cut(s)	274, 359, 1225
BsmFI	GGGAC	4 cut(s)	729, 1049, 2180, 2385
BsmI	GAATGC	1 cut(s)	622
Bso31I	GGTCTC	1 cut(s)	832
Bsp1286I	GDGCHC	4 cut(s)	669, 903, 1549, 2606
Bsp143I	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
Bsp68I	TCGCGA	1 cut(s)	301
BspCNI	CTCAG	7 cut(s)	375, 1019, 1235, 1245, 1412, 1751, 2101
BspDI	ATCGAT	2 cut(s)	1957, 2402
BspFNI	CGCG	3 cut(s)	203, 301, 458
BspHI	TCATGA	1 cut(s)	1930
BspMAI	CTGCAG	2 cut(s)	195, 2284
BspMI	ACCTGC	1 cut(s)	1708
BspPI	GGATC	6 cut(s)	607, 658, 772, 792, 805, 941
BspQI	GCTCTTC	1 cut(s)	2364
BspT107I	GGYRCC	1 cut(s)	232
BspTNI	GGTCTC	1 cut(s)	832
BsrBI	CCGCTC	1 cut(s)	407
BsrDI	GCAATG	5 cut(s)	639, 1490, 1713, 2304, 2526
BsrFI	RCCGGY	1 cut(s)	877
BsrI	ACTGG	7 cut(s)	996, 1023, 1279, 1554, 1647, 1838, 2451
BssAI	RCCGGY	1 cut(s)	877
BssECI	CCNNGG	6 cut(s)	201, 222, 414, 801, 904, 1125
BssMI	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
BssNAI	GTATAC	2 cut(s)	1381, 2041
BssNI	GRCGYC	3 cut(s)	233, 251, 267
BssT1I	CCWWGG	1 cut(s)	904
Bst1107I	GTATAC	2 cut(s)	1381, 2041
Bst2UI	CCWGG	3 cut(s)	803, 1110, 1866
Bst4CI	ACNGT	4 cut(s)	1033, 1219, 2119, 2152
Bst6I	CTCTTC	5 cut(s)	542, 837, 1129, 2169, 2364
BstACI	GRCGYC	3 cut(s)	233, 251, 267
BstAPI	GCANNNNNTGC	1 cut(s)	1987
BstC8I	GCNNGC	5 cut(s)	191, 195, 1353, 1983, 2096
BstDEI	CTNAG	7 cut(s)	383, 1006, 1222, 1253, 1399, 1738, 2109
BstDSI	CCRYGG	4 cut(s)	201, 222, 414, 1125
BstFNI	CGCG	3 cut(s)	203, 301, 458
BstH2I	RGCGCY	1 cut(s)	236
BstHHI	GCGC	2 cut(s)	235, 2326
BstKTI	GATC	8 cut(s)	602, 653, 767, 800, 936, 1657, 1957, 1961
BstMAI	GTCTC	5 cut(s)	274, 359, 720, 832, 1225
BstMBI	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
BstMCI	CGRYCG	2 cut(s)	85, 331
BstMWI	GCNNNNNNNGC	8 cut(s)	203, 227, 704, 883, 1987, 2192, 2393, 2638
BstNI	CCWGG	3 cut(s)	803, 1110, 1866
BstNSI	RCATGY	2 cut(s)	358, 1985
BstSCI	CCNGG	6 cut(s)	272, 476, 718, 801, 1108, 1864
BstSFI	CTRYAG	5 cut(s)	191, 1764, 2190, 2280, 2636
BstSLI	GKGCMC	1 cut(s)	1549
BstUI	CGCG	3 cut(s)	203, 301, 458
BstX2I	RGATCY	1 cut(s)	797
BstXI	CCANNNNNNTGG	1 cut(s)	1822
BstYI	RGATCY	1 cut(s)	797
BstZ17I	GTATAC	2 cut(s)	1381, 2041
Bsu15I	ATCGAT	2 cut(s)	1957, 2402
BsuI	GTATCC	1 cut(s)	1844
BsuTUI	ATCGAT	2 cut(s)	1957, 2402
BtgI	CCRYGG	4 cut(s)	201, 222, 414, 1125
BtgZI	GCGATG	1 cut(s)	122
BtrI	CACGTC	1 cut(s)	2051
BtsI	GCAGTG	1 cut(s)	1914
BtsIMutI	CAGTG	7 cut(s)	968, 1029, 1030, 1547, 1686, 1914, 2444
BtuMI	TCGCGA	1 cut(s)	301
BveI	ACCTGC	1 cut(s)	1708
Cac8I	GCNNGC	5 cut(s)	191, 195, 1353, 1983, 2096
CciI	TCATGA	1 cut(s)	1930
CfoI	GCGC	2 cut(s)	235, 2326
Cfr10I	RCCGGY	1 cut(s)	877
Cfr13I	GGNCC	7 cut(s)	40, 205, 716, 1063, 1247, 1370, 1475
Cfr42I	CCGCGG	1 cut(s)	204
ClaI	ATCGAT	2 cut(s)	1957, 2402
CseI	GACGC	1 cut(s)	240
CsiI	ACCWGGT	1 cut(s)	1864
Csp6I	GTAC	4 cut(s)	1034, 1423, 1773, 2540
CviQI	GTAC	4 cut(s)	1034, 1423, 1773, 2540
DdeI	CTNAG	7 cut(s)	383, 1006, 1222, 1253, 1399, 1738, 2109
DinI	GGCGCC	1 cut(s)	234
DpnI	GATC	8 cut(s)	601, 652, 766, 799, 935, 1656, 1956, 1960
DpnII	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
DraIII	CACNNNGTG	1 cut(s)	343
DrdI	GACNNNNNNGTC	1 cut(s)	332
DseDI	GACNNNNNNGTC	1 cut(s)	332
EaeI	YGGCCR	2 cut(s)	429, 1644
Eam1104I	CTCTTC	5 cut(s)	542, 837, 1129, 2169, 2364
EarI	CTCTTC	5 cut(s)	542, 837, 1129, 2169, 2364
EciI	GGCGGA	1 cut(s)	434
Ecl136II	GAGCTC	1 cut(s)	2604
Eco130I	CCWWGG	1 cut(s)	904
Eco24I	GRGCYC	1 cut(s)	2606
Eco31I	GGTCTC	1 cut(s)	832
Eco47I	GGWCC	5 cut(s)	40, 716, 1063, 1247, 1370
Eco53kI	GAGCTC	1 cut(s)	2604
Eco57I	CTGAAG	2 cut(s)	1710, 2345
EcoICRI	GAGCTC	1 cut(s)	2604
EcoO109I	RGGNCCY	2 cut(s)	716, 1063
EcoRI	GAATTC	2 cut(s)	633, 778
EcoRII	CCWGG	3 cut(s)	801, 1108, 1864
EcoT14I	CCWWGG	1 cut(s)	904
EcoT38I	GRGCYC	1 cut(s)	2606
EgeI	GGCGCC	1 cut(s)	234
EheI	GGCGCC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	904
Esp3I	CGTCTC	3 cut(s)	274, 359, 1225
FalI	AAGNNNNNCTT	2 cut(s)	1596, 1628
FaqI	GGGAC	4 cut(s)	729, 1049, 2180, 2385
FauI	CCCGC	1 cut(s)	2091
FblI	GTMKAC	3 cut(s)	81, 1380, 2040
FriOI	GRGCYC	1 cut(s)	2606
FspBI	CTAG	9 cut(s)	905, 945, 1511, 1577, 1710, 1976, 2124, 2268, 2661
GlaI	GCGC	2 cut(s)	234, 2325
GsaI	CCCAGC	1 cut(s)	1476
GsuI	CTGGAG	5 cut(s)	166, 1006, 2114, 2277, 2556
HaeII	RGCGCY	1 cut(s)	236
HapII	CCGG	8 cut(s)	38, 151, 273, 389, 432, 477, 720, 878
HgaI	GACGC	1 cut(s)	240
HhaI	GCGC	2 cut(s)	235, 2326
Hin1I	GRCGYC	3 cut(s)	233, 251, 267
Hin6I	GCGC	2 cut(s)	233, 2324
HinP1I	GCGC	2 cut(s)	233, 2324
HincII	GTYRAC	5 cut(s)	82, 352, 360, 441, 2238
HindII	GTYRAC	5 cut(s)	82, 352, 360, 441, 2238
HpaII	CCGG	8 cut(s)	38, 151, 273, 389, 432, 477, 720, 878
HphI	GGTGA	5 cut(s)	101, 532, 556, 759, 2318
Hpy188III	TCNNGA	8 cut(s)	115, 300, 917, 987, 1448, 1931, 1942, 2256
Hpy99I	CGWCG	4 cut(s)	129, 256, 269, 2348
HpyCH4III	ACNGT	4 cut(s)	1033, 1219, 2119, 2152
HpyCH4IV	ACGT	6 cut(s)	267, 910, 2043, 2050, 2346, 2511
HpyF10VI	GCNNNNNNNGC	8 cut(s)	203, 227, 704, 883, 1987, 2192, 2393, 2638
HpyF3I	CTNAG	7 cut(s)	383, 1006, 1222, 1253, 1399, 1738, 2109
HpySE526I	ACGT	6 cut(s)	267, 910, 2043, 2050, 2346, 2511
Hsp92I	GRCGYC	3 cut(s)	233, 251, 267
HspAI	GCGC	2 cut(s)	233, 2324
KasI	GGCGCC	1 cut(s)	232
KflI	GGGWCCC	1 cut(s)	716
KspI	CCGCGG	1 cut(s)	204
Kzo9I	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
LguI	GCTCTTC	1 cut(s)	2364
LmnI	GCTCC	9 cut(s)	54, 404, 672, 906, 1046, 1283, 1595, 1622, 2258
MabI	ACCWGGT	1 cut(s)	1864
MaeI	CTAG	9 cut(s)	905, 945, 1511, 1577, 1710, 1976, 2124, 2268, 2661
MaeII	ACGT	6 cut(s)	267, 910, 2043, 2050, 2346, 2511
MaeIII	GTNAC	6 cut(s)	487, 966, 1025, 1840, 2119, 2617
MalI	GATC	8 cut(s)	601, 652, 766, 799, 935, 1656, 1956, 1960
MbiI	CCGCTC	1 cut(s)	407
MboI	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
MfeI	CAATTG	2 cut(s)	1788, 2034
MflI	RGATCY	1 cut(s)	797
MhlI	GDGCHC	4 cut(s)	669, 903, 1549, 2606
MlsI	TGGCCA	1 cut(s)	1646
MluNI	TGGCCA	1 cut(s)	1646
Mly113I	GGCGCC	1 cut(s)	233
MmeI	TCCRAC	3 cut(s)	2026, 2112, 2185
Mox20I	TGGCCA	1 cut(s)	1646
MroXI	GAANNNNTTC	3 cut(s)	1188, 1746, 1926
MscI	TGGCCA	1 cut(s)	1646
MseI	TTAA	6 cut(s)	378, 837, 1200, 1805, 1856, 2274
MslI	CAYNNNNRTG	1 cut(s)	1388
Msp20I	TGGCCA	1 cut(s)	1646
MspA1I	CMGCKG	3 cut(s)	186, 203, 1226
MspI	CCGG	8 cut(s)	38, 151, 273, 389, 432, 477, 720, 878
MspR9I	CCNGG	6 cut(s)	274, 478, 720, 803, 1110, 1866
MunI	CAATTG	2 cut(s)	1788, 2034
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	3 cut(s)	803, 1110, 1866
MvnI	CGCG	3 cut(s)	203, 301, 458
MwoI	GCNNNNNNNGC	8 cut(s)	203, 227, 704, 883, 1987, 2192, 2393, 2638
NarI	GGCGCC	1 cut(s)	233
NciI	CCSGG	3 cut(s)	274, 478, 720
NdeII	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
NmuCI	GTSAC	4 cut(s)	487, 966, 1025, 1840
NruI	TCGCGA	1 cut(s)	301
NspI	RCATGY	2 cut(s)	358, 1985
PaeI	GCATGC	1 cut(s)	1985
PagI	TCATGA	1 cut(s)	1930
PciI	ACATGT	1 cut(s)	354
PciSI	GCTCTTC	1 cut(s)	2364
PcsI	WCGNNNNNNNCGW	1 cut(s)	41
PctI	GAATGC	1 cut(s)	622
PdmI	GAANNNNTTC	3 cut(s)	1188, 1746, 1926
PfeI	GAWTC	7 cut(s)	238, 453, 1016, 1091, 1184, 1358, 1487
PflFI	GACNNNGTC	1 cut(s)	332
PflMI	CCANNNNNTGG	2 cut(s)	343, 1871
PfoI	TCCNGGA	2 cut(s)	272, 476
PluTI	GGCGCC	1 cut(s)	236
PpuMI	RGGWCCY	2 cut(s)	716, 1063
PscI	ACATGT	1 cut(s)	354
Psp124BI	GAGCTC	1 cut(s)	2606
Psp5II	RGGWCCY	2 cut(s)	716, 1063
Psp6I	CCWGG	3 cut(s)	801, 1108, 1864
PspFI	CCCAGC	1 cut(s)	1472
PspGI	CCWGG	3 cut(s)	801, 1108, 1864
PspPI	GGNCC	7 cut(s)	40, 205, 716, 1063, 1247, 1370, 1475
PspPPI	RGGWCCY	2 cut(s)	716, 1063
PstI	CTGCAG	2 cut(s)	195, 2284
PsuI	RGATCY	1 cut(s)	797
PsyI	GACNNNGTC	1 cut(s)	332
PvuII	CAGCTG	2 cut(s)	186, 1226
RruI	TCGCGA	1 cut(s)	301
RsaI	GTAC	4 cut(s)	1035, 1424, 1774, 2541
RsaNI	GTAC	4 cut(s)	1034, 1423, 1773, 2540
RseI	CAYNNNNRTG	1 cut(s)	1388
SacI	GAGCTC	1 cut(s)	2606
SacII	CCGCGG	1 cut(s)	204
SalI	GTCGAC	1 cut(s)	80
SapI	GCTCTTC	1 cut(s)	2364
SaqAI	TTAA	6 cut(s)	378, 837, 1200, 1805, 1856, 2274
Sau3AI	GATC	8 cut(s)	599, 650, 764, 797, 933, 1654, 1954, 1958
Sau96I	GGNCC	7 cut(s)	40, 205, 716, 1063, 1247, 1370, 1475
SbfI	CCTGCAGG	1 cut(s)	195
ScaI	AGTACT	1 cut(s)	2541
ScrFI	CCNGG	6 cut(s)	274, 478, 720, 803, 1110, 1866
SdaI	CCTGCAGG	1 cut(s)	195
SduI	GDGCHC	4 cut(s)	669, 903, 1549, 2606
SexAI	ACCWGGT	1 cut(s)	1864
SfcI	CTRYAG	5 cut(s)	191, 1764, 2190, 2280, 2636
SfiI	GGCCNNNNNGGCC	1 cut(s)	203
SfoI	GGCGCC	1 cut(s)	234
Sfr303I	CCGCGG	1 cut(s)	204
SgrBI	CCGCGG	1 cut(s)	204
SinI	GGWCC	5 cut(s)	40, 716, 1063, 1247, 1370
SmiMI	CAYNNNNRTG	1 cut(s)	1388
SmlI	CTYRAG	2 cut(s)	1323, 2599
SmoI	CTYRAG	2 cut(s)	1323, 2599
SphI	GCATGC	1 cut(s)	1985
Sse8387I	CCTGCAGG	1 cut(s)	195
SspDI	GGCGCC	1 cut(s)	232
SspMI	CTAG	9 cut(s)	905, 945, 1511, 1577, 1710, 1976, 2124, 2268, 2661
SstI	GAGCTC	1 cut(s)	2606
StyD4I	CCNGG	6 cut(s)	272, 476, 718, 801, 1108, 1864
StyI	CCWWGG	1 cut(s)	904
TaaI	ACNGT	4 cut(s)	1033, 1219, 2119, 2152
TaiI	ACGT	6 cut(s)	270, 913, 2046, 2053, 2349, 2514
TaqI	TCGA	6 cut(s)	81, 161, 264, 1957, 2402, 2606
TaqII	GACCGA	2 cut(s)	345, 377
TatI	WGTACW	3 cut(s)	1033, 1772, 2539
TauI	GCSGC	8 cut(s)	200, 203, 206, 230, 233, 288, 291, 461
TfiI	GAWTC	7 cut(s)	238, 453, 1016, 1091, 1184, 1358, 1487
Tru1I	TTAA	6 cut(s)	378, 837, 1200, 1805, 1856, 2274
Tru9I	TTAA	6 cut(s)	378, 837, 1200, 1805, 1856, 2274
TscAI	CASTG	7 cut(s)	975, 1030, 1036, 1554, 1693, 1914, 2451
TseFI	GTSAC	4 cut(s)	487, 966, 1025, 1840
Tsp45I	GTSAC	4 cut(s)	487, 966, 1025, 1840
TspDTI	ATGAA	8 cut(s)	20, 791, 968, 1347, 1479, 1919, 2232, 2633
TspGWI	ACGGA	2 cut(s)	458, 506
TspRI	CASTG	7 cut(s)	975, 1030, 1036, 1554, 1693, 1914, 2451
Tth111I	GACNNNGTC	1 cut(s)	332
Van91I	CCANNNNNTGG	2 cut(s)	343, 1871
VneI	GTGCAC	1 cut(s)	1545
VpaK11BI	GGWCC	5 cut(s)	40, 716, 1063, 1247, 1370
XapI	RAATTY	5 cut(s)	633, 778, 1743, 2219, 2485
XceI	RCATGY	2 cut(s)	358, 1985
XmaJI	CCTAGG	1 cut(s)	904
XmiI	GTMKAC	3 cut(s)	81, 1380, 2040
XmnI	GAANNNNTTC	3 cut(s)	1188, 1746, 1926
XspI	CTAG	9 cut(s)	905, 945, 1511, 1577, 1710, 1976, 2124, 2268, 2661
ZraI	GACGTC	1 cut(s)	268
ZrmI	AGTACT	1 cut(s)	2541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.