Rroxscaffold_4G00331840

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003320
Physical Location & Seq
Reverse (-)
65837844 .. 65840113
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331840.1

Sequence Viewer

Length: 699 bp
ATGGCAGTTGCATTCCATGACATCAACTCGGCTGCTGGCCTCAAGAAATTGGACCAGTACTTGCTCACTCGCAGTTACATCACCGGCTATCAGGCTTCGAAGGATGATGTCACTGTGCATGCTGCTCTGTCCAAGCCTCCATCAGCTGAATACGTGAATGCATCTCGGTGGTACAACCACATTGCTGCACTTCTTAGGATTTCGGGTGTTTCTGGAGAAGGTTGTGGTGTCACAATTGAGGGATCTGCTCCCATCACAGCAGAAGCCGTTGCCACACCTCCAGCTGGTGATTCAAAGGCCTCTGCTGCTGAAGATGATGATGATAGTGATGTGGATCTCTTTGGTGAAGAGACTGAAGAGGAGAAGAAGGCTGCCGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAAGAGTCTGGCAAGTCATCAGTTCTATTGGATATTAAGCCATGGGATGATGAAACAGACATGAAAAAGCTTGAGGAGGCAGTAAGGAGCATTGAGGTTGAGGGCTTACATTGGGGAGCATCTAAACTAGTACCTGTTGGGTATGGCATAAAGAAGTTGACAATTATGCTTACAATTGTTGATGACCTTGTTTCTGTGGACTCTCTGATTGAAGAACGTCTTACAGTGGAACCTATTAATGAATATGTCCAGAGCTGTGACATTGTTGCCTTCAATAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

232

Amino Acids

24.99

Weight (kDa)

4.66

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 145 - 232 1.8e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 250, 342
AcuI CTGAAG 2 cut(s) 330, 375
AfaI GTAC 3 cut(s) 59, 173, 546
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 3 cut(s) 294, 626, 688
AhlI ACTAGT 1 cut(s) 541
AluBI AGCT 5 cut(s) 146, 284, 389, 484, 669
AluI AGCT 5 cut(s) 146, 284, 389, 484, 669
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 2 cut(s) 250, 342
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 2 cut(s) 37, 297
ApeKI GCWGC 7 cut(s) 32, 122, 185, 305, 371, 386, 389
AseI ATTAAT 1 cut(s) 651
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 73, 299, 356
AsuII TTCGAA 1 cut(s) 98
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BccI CCATC 2 cut(s) 148, 260
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BcoDI GTCTC 1 cut(s) 344
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 1 cut(s) 542
BisI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
BlsI GCNGC 7 cut(s) 34, 124, 187, 307, 373, 388, 391
BmcAI AGTACT 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 645
BmsI GCATC 3 cut(s) 170, 410, 542
BpmI CTGGAG 2 cut(s) 234, 264
Bpu14I TTCGAA 1 cut(s) 98
BpuEI CTTGAG 2 cut(s) 26, 506
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 455
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 455
BseGI GGATG 2 cut(s) 109, 466
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 55
BseRI GAGGAG 2 cut(s) 374, 503
BseXI GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BsgI GTGCAG 2 cut(s) 171, 405
BshFI GGCC 2 cut(s) 39, 299
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 2 cut(s) 11, 163
BsnI GGCC 2 cut(s) 39, 299
Bsp119I TTCGAA 1 cut(s) 98
Bsp143I GATC 2 cut(s) 242, 334
Bsp19I CCATGG 1 cut(s) 455
BspANI GGCC 2 cut(s) 39, 299
BspLI GGNNCC 1 cut(s) 645
BspPI GGATC 2 cut(s) 250, 342
BspT104I TTCGAA 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 2 cut(s) 242, 334
BssT1I CCWWGG 1 cut(s) 455
Bst4CI ACNGT 2 cut(s) 115, 640
Bst6I CTCTTC 2 cut(s) 342, 351
BstBAI YACGTR 1 cut(s) 154
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 455
BstF5I GGATG 2 cut(s) 109, 466
BstKTI GATC 2 cut(s) 245, 337
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 242, 334
BstMWI GCNNNNNNNGC 2 cut(s) 305, 398
BstNSI RCATGY 1 cut(s) 122
BstV1I GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BstX2I RGATCY 2 cut(s) 242, 334
BstYI RGATCY 2 cut(s) 242, 334
BsuRI GGCC 2 cut(s) 39, 299
BtgI CCRYGG 1 cut(s) 455
BtsCI GGATG 2 cut(s) 109, 466
BtsIMutI CAGTG 2 cut(s) 111, 645
Cac8I GCNNGC 2 cut(s) 37, 120
CaiI CAGNNNCTG 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 3 cut(s) 58, 172, 545
CviAII CATG 4 cut(s) 17, 119, 456, 475
CviQI GTAC 3 cut(s) 58, 172, 545
DdeI CTNAG 1 cut(s) 194
DpnI GATC 2 cut(s) 244, 336
DpnII GATC 2 cut(s) 242, 334
Eam1104I CTCTTC 2 cut(s) 342, 351
EarI CTCTTC 2 cut(s) 342, 351
Eco130I CCWWGG 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 330, 375
EcoT14I CCWWGG 1 cut(s) 455
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 4 cut(s) 20, 122, 459, 478
FaiI YATR 9 cut(s) 18, 120, 457, 476, 558, 563, 581, 660, 697
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 4 cut(s) 16, 118, 455, 474
Fnu4HI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
FokI GGATG 2 cut(s) 116, 473
Fsp4HI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
FspBI CTAG 1 cut(s) 542
GluI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
GsuI CTGGAG 2 cut(s) 234, 264
HaeIII GGCC 2 cut(s) 39, 299
HapII CCGG 1 cut(s) 84
Hin1II CATG 4 cut(s) 20, 122, 459, 478
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 3 cut(s) 290, 419, 614
HpaII CCGG 1 cut(s) 84
HphI GGTGA 3 cut(s) 73, 299, 356
Hpy166II GTNNAC 2 cut(s) 573, 613
Hpy188I TCNGA 1 cut(s) 621
Hpy188III TCNNGA 3 cut(s) 43, 213, 664
Hpy8I GTNNAC 2 cut(s) 573, 613
HpyAV CCTTC 4 cut(s) 94, 212, 361, 694
HpyCH4III ACNGT 2 cut(s) 115, 640
HpyCH4IV ACGT 3 cut(s) 153, 382, 631
HpyCH4V TGCA 5 cut(s) 11, 118, 161, 188, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 398
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 3 cut(s) 153, 382, 631
Hsp92II CATG 4 cut(s) 20, 122, 459, 478
Kzo9I GATC 2 cut(s) 242, 334
LmnI GCTCC 3 cut(s) 253, 501, 530
Lsp1109I GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
LweI GCATC 3 cut(s) 170, 410, 542
MaeI CTAG 1 cut(s) 542
MaeII ACGT 3 cut(s) 153, 382, 631
MaeIII GTNAC 4 cut(s) 74, 109, 229, 671
MalI GATC 2 cut(s) 244, 336
MboI GATC 2 cut(s) 242, 334
MboII GAAGA 6 cut(s) 323, 359, 368, 376, 389, 638
MfeI CAATTG 2 cut(s) 234, 588
MflI RGATCY 2 cut(s) 242, 334
MluCI AATT 4 cut(s) 47, 234, 576, 588
MlyI GAGTC 2 cut(s) 428, 608
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 2 cut(s) 450, 651
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 3 cut(s) 146, 284, 389
MspI CCGG 1 cut(s) 84
MunI CAATTG 2 cut(s) 234, 588
Mva1269I GAATGC 2 cut(s) 11, 163
MwoI GCNNNNNNNGC 2 cut(s) 305, 398
NcoI CCATGG 1 cut(s) 455
NdeII GATC 2 cut(s) 242, 334
NlaIII CATG 4 cut(s) 20, 122, 459, 478
NlaIV GGNNCC 1 cut(s) 645
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 3 cut(s) 109, 229, 671
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 98
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 2 cut(s) 11, 163
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 7 cut(s) 34, 124, 187, 307, 373, 388, 391
PleI GAGTC 2 cut(s) 427, 608
PpsI GAGTC 2 cut(s) 427, 608
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 651
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 242, 334
PvuII CAGCTG 3 cut(s) 146, 284, 389
RsaI GTAC 3 cut(s) 59, 173, 546
RsaNI GTAC 3 cut(s) 58, 172, 545
RseI CAYNNNNRTG 1 cut(s) 166
SaqAI TTAA 2 cut(s) 450, 651
SatI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
Sau3AI GATC 2 cut(s) 242, 334
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 2 cut(s) 428, 608
SfaNI GCATC 3 cut(s) 170, 410, 542
SfuI TTCGAA 1 cut(s) 98
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 41, 485
SmoI CTYRAG 2 cut(s) 41, 485
SpeI ACTAGT 1 cut(s) 541
SphI GCATGC 1 cut(s) 122
Sse9I AATT 4 cut(s) 47, 234, 576, 588
SseBI AGGCCT 1 cut(s) 299
SspMI CTAG 1 cut(s) 542
StuI AGGCCT 1 cut(s) 299
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 115, 640
TaiI ACGT 3 cut(s) 156, 385, 634
TaqI TCGA 1 cut(s) 98
TasI AATT 4 cut(s) 47, 234, 576, 588
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 450, 651
Tru9I TTAA 2 cut(s) 450, 651
TscAI CASTG 2 cut(s) 118, 645
TseFI GTSAC 3 cut(s) 109, 229, 671
TseI GCWGC 7 cut(s) 32, 122, 185, 305, 371, 386, 389
Tsp45I GTSAC 3 cut(s) 109, 229, 671
TspDTI ATGAA 3 cut(s) 480, 491, 669
TspRI CASTG 2 cut(s) 118, 645
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 651
XceI RCATGY 1 cut(s) 122
XspI CTAG 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 59
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.