AT2G18110

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003320
Physical Location & Seq
Forward (+)
7872201 .. 7874010
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18110.1

Sequence Viewer

Length: 696 bp
ATGGCGGCTTTCCCCAACCTCAACTCTGGCTCCGGATTGAAGAAGCTCGATGAGCATCTTCTCACTCGCAGTTACATCACTGGGTACCAAGCTTCAAAGGATGATATCACTGTGTTTACAGCTCTTTCAAAGCCCCCAACTTCAGAGTTTGTCAATGTATCTCGTTGGTTCAACCACATCGATGCCCTCTTGAGGATCTCTGGTGTCTCTGCTGAAGGAAGTGGTGTCATTGTTGAAGGGTCATCACCTATCACAGAAGAGGCTGTTGCTACTCCCCCTGCAGCTGATTCTAAGGATACTGCTGCTGAAGAAGAAGATGATGATGATGTTGACCTTTTCGGGGAGGAGACCGAAGAGGAAAAGAAAGCTGCTGAAGAGAGAGCAGCTTCTGTGAAGGCATCTACAAAGAAGAAAGAATCCGGAAAGTCATCAGTTTTGATGGATATCAAGCCGTGGGATGATGAGACGGACATGAAGAAGCTAGAGGAAGCTGTAAGATCCATTCAGATGGAAGGATTGTTTTGGGGAGCATCAAAGCTTGTACCCGTTGGTTACGGTATCAAGAAGTTGCACATCATGTGCACCATTGTTGATGACCTTGTATCTATCGACACCATGATCGAAGAGCAACTCACTGTTGAACCGATCAATGAATATGTCCAGAGTTGTGACATTGTTGCCTTCAACAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

231

Amino Acids

25.27

Weight (kDa)

4.49

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 144 - 231 2.2e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 84
AccIII TCCGGA 2 cut(s) 32, 419
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 203, 492
AcuI CTGAAG 4 cut(s) 126, 234, 327, 393
AfaI GTAC 2 cut(s) 86, 543
AfiI CCNNNNNNNGG 2 cut(s) 192, 340
AgsI TTSAA 7 cut(s) 40, 96, 129, 172, 236, 641, 685
AluBI AGCT 9 cut(s) 46, 92, 122, 284, 368, 386, 481, 491, 538
AluI AGCT 9 cut(s) 46, 92, 122, 284, 368, 386, 481, 491, 538
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 3 cut(s) 211, 341, 458
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 2 cut(s) 203, 492
AlwNI CAGNNNCTG 1 cut(s) 389
Aor13HI TCCGGA 2 cut(s) 32, 419
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 4 cut(s) 281, 302, 368, 383
Asp718I GGTACC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 4 cut(s) 289, 293, 355, 395
BccI CCATC 2 cut(s) 433, 502
BceAI ACGGC 1 cut(s) 436
BciVI GTATCC 1 cut(s) 289
BcoDI GTCTC 3 cut(s) 211, 341, 458
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 279
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 5 cut(s) 6, 282, 303, 369, 384
BlsI GCNGC 5 cut(s) 7, 283, 304, 370, 385
BmiI GGNNCC 2 cut(s) 31, 86
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 4 cut(s) 64, 172, 407, 539
BmuI ACTGGG 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 211
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 2 cut(s) 54, 443
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 452
BsaWI WCCGGW 2 cut(s) 32, 419
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 340
Bse1I ACTGG 1 cut(s) 85
Bse8I GATNNNNATC 2 cut(s) 54, 443
BseAI TCCGGA 2 cut(s) 32, 419
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 2 cut(s) 106, 463
BseJI GATNNNNATC 2 cut(s) 54, 443
BseLI CCNNNNNNNGG 2 cut(s) 192, 340
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 584
BseXI GCAGC 4 cut(s) 289, 293, 355, 395
BshNI GGYRCC 1 cut(s) 84
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 584
BsiSI CCGG 2 cut(s) 33, 420
BslI CCNNNNNNNGG 2 cut(s) 192, 340
BsmAI GTCTC 3 cut(s) 211, 341, 458
BsmBI CGTCTC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 584
Bsp13I TCCGGA 2 cut(s) 32, 419
Bsp143I GATC 4 cut(s) 195, 497, 618, 645
BspACI CCGC 1 cut(s) 5
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 2 cut(s) 32, 419
BspLI GGNNCC 2 cut(s) 31, 86
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 203, 492
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 341
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 4 cut(s) 195, 497, 618, 645
Bst4CI ACNGT 3 cut(s) 112, 557, 637
Bst6I CTCTTC 4 cut(s) 252, 348, 369, 618
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 106, 463
BstKTI GATC 4 cut(s) 198, 500, 621, 648
BstMAI GTCTC 3 cut(s) 211, 341, 458
BstMBI GATC 4 cut(s) 195, 497, 618, 645
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 584
BstV1I GCAGC 4 cut(s) 289, 293, 355, 395
BstX2I RGATCY 2 cut(s) 195, 497
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 2 cut(s) 195, 497
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 1 cut(s) 289
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 2 cut(s) 106, 463
BtsIMutI CAGTG 3 cut(s) 78, 108, 633
CaiI CAGNNNCTG 1 cut(s) 389
ClaI ATCGAT 1 cut(s) 180
Csp6I GTAC 2 cut(s) 85, 542
CviAII CATG 3 cut(s) 472, 577, 616
CviQI GTAC 2 cut(s) 85, 542
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 197, 499, 620, 647
DpnII GATC 4 cut(s) 195, 497, 618, 645
Eam1104I CTCTTC 4 cut(s) 252, 348, 369, 618
EarI CTCTTC 4 cut(s) 252, 348, 369, 618
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 2 cut(s) 106, 445
Eco57I CTGAAG 4 cut(s) 126, 234, 327, 393
EcoRV GATATC 2 cut(s) 106, 445
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 3 cut(s) 475, 580, 619
FaiI YATR 5 cut(s) 473, 578, 617, 657, 694
FatI CATG 3 cut(s) 471, 576, 615
Fnu4HI GCNGC 5 cut(s) 6, 282, 303, 369, 384
FokI GGATG 2 cut(s) 113, 470
Fsp4HI GCNGC 5 cut(s) 6, 282, 303, 369, 384
FspBI CTAG 1 cut(s) 482
GluI GCNGC 5 cut(s) 6, 282, 303, 369, 384
HapII CCGG 2 cut(s) 33, 420
Hin1II CATG 3 cut(s) 475, 580, 619
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 2 cut(s) 90, 536
HinfI GANTC 2 cut(s) 287, 416
HpaII CCGG 2 cut(s) 33, 420
HphI GGTGA 1 cut(s) 237
Hpy166II GTNNAC 3 cut(s) 117, 331, 582
Hpy188I TCNGA 2 cut(s) 145, 507
Hpy188III TCNNGA 5 cut(s) 33, 190, 420, 562, 661
Hpy8I GTNNAC 3 cut(s) 117, 331, 582
HpyAV CCTTC 5 cut(s) 209, 230, 388, 506, 691
HpyCH4III ACNGT 3 cut(s) 112, 557, 637
HpyCH4V TGCA 3 cut(s) 281, 571, 582
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 1 cut(s) 291
Hsp92II CATG 3 cut(s) 475, 580, 619
Kpn2I TCCGGA 2 cut(s) 32, 419
KpnI GGTACC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 195, 497, 618, 645
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 2 cut(s) 35, 527
LpnPI CCDG 7 cut(s) 12, 46, 66, 186, 291, 433, 674
Lsp1109I GCAGC 4 cut(s) 289, 293, 355, 395
LweI GCATC 4 cut(s) 64, 172, 407, 539
MaeI CTAG 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 71, 551, 668
MalI GATC 4 cut(s) 197, 499, 620, 647
MboI GATC 4 cut(s) 195, 497, 618, 645
MflI RGATCY 2 cut(s) 195, 497
MhlI GDGCHC 1 cut(s) 584
MnlI CCTC 7 cut(s) 29, 186, 197, 253, 337, 349, 478
MroI TCCGGA 2 cut(s) 32, 419
MslI CAYNNNNRTG 2 cut(s) 180, 506
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 2 cut(s) 33, 420
MwoI GCNNNNNNNGC 1 cut(s) 52
NdeII GATC 4 cut(s) 195, 497, 618, 645
NlaIII CATG 3 cut(s) 475, 580, 619
NlaIV GGNNCC 2 cut(s) 31, 86
NmuCI GTSAC 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 618
PfeI GAWTC 2 cut(s) 287, 416
PkrI GCNGC 5 cut(s) 7, 283, 304, 370, 385
PspN4I GGNNCC 2 cut(s) 31, 86
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 2 cut(s) 195, 497
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 86, 543
RsaNI GTAC 2 cut(s) 85, 542
RseI CAYNNNNRTG 2 cut(s) 180, 506
SapI GCTCTTC 1 cut(s) 618
SatI GCNGC 5 cut(s) 6, 282, 303, 369, 384
Sau3AI GATC 4 cut(s) 195, 497, 618, 645
SduI GDGCHC 1 cut(s) 584
SfaNI GCATC 4 cut(s) 64, 172, 407, 539
SfcI CTRYAG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 180, 506
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 482
TaaI ACNGT 3 cut(s) 112, 557, 637
TaqI TCGA 4 cut(s) 48, 180, 609, 621
TaqII GACCGA 1 cut(s) 365
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 287, 416
TscAI CASTG 3 cut(s) 85, 115, 640
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 4 cut(s) 281, 302, 368, 383
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 2 cut(s) 488, 666
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 3 cut(s) 85, 115, 640
VneI GTGCAC 1 cut(s) 580
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.