Rw7G009550

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003320
Physical Location & Seq
Reverse (-)
8918943 .. 8920364
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009550.1

Sequence Viewer

Length: 654 bp
ATGGAATCCTTGACTGTTAATTTGTTCCTTACTTTCTCGGAGTTACATCACTGGGTAAACTTTTTTTGTCAGTATCAGGCTTCAAAGGATGATATCACTGTGCTTGCAGCTCTTGCAAAACCCCCATCCTCGGAATATGTGAATGTGCTGCGATGGTACAAACATATTGAGGCACTTCTGAGGATTTCTGGTGTTTCCGGAGAAGGTTGTGGTGTCACCATCAAGGGAGATGCTCCTGCCTCAGCTGCTGAAGAAGATGATGATGATGTGGATCTTTTTGGTGAAGAAACCGAAGAGGAGAAGAAGGCTGCTCAAGAACGTGCAGCCTGCATCAAGGCATCTGCAAAAAAGAGATCGTCAGTTCTGATGGATGTCAAACCATGGGATGATGAAACCGACATGCAAAAACTTTTGGAAGCAGTAAAAAATGTTCAGATGGAAGGTTTGCATTGGGGAGCATCCAAACTTGTACCTGTTGGGTACGGAATAAAGAAGTTGCAGATAATGCTAACAATTGTTGATGACCTGGTCTCTGTGGACACTCTCATTGAGGAACATCTCACAGTTGAACCAATTAATGAGTATGTCCAAAGCTGCGATATTGTAGCCTTCAACAAAATATGTACGTATAGTTTCCTTGTACTTTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

217

Amino Acids

24.23

Weight (kDa)

4.64

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 120 - 207 4.5e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 197
AclWI GGATC 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 5 cut(s) 158, 471, 482, 625, 642
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 3 cut(s) 84, 569, 613
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 3 cut(s) 110, 245, 594
AluI AGCT 3 cut(s) 110, 245, 594
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 197
ApeKI GCWGC 6 cut(s) 107, 148, 245, 308, 323, 594
ArsI GACNNNNNNTTYG 1 cut(s) 36
AseI ATTAAT 1 cut(s) 576
AsuHPI GGTGA 2 cut(s) 208, 293
BbvCI CCTCAGC 1 cut(s) 241
BbvI GCAGC 6 cut(s) 119, 135, 232, 295, 335, 581
BccI CCATC 5 cut(s) 133, 147, 227, 361, 430
BciT130I CCWGG 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 535
BisI GCNGC 6 cut(s) 108, 149, 246, 309, 324, 595
BlsI GCNGC 6 cut(s) 109, 150, 247, 310, 325, 596
Bme1390I CCNGG 1 cut(s) 527
BmrFI CCNGG 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 4 cut(s) 220, 339, 347, 467
BmuI ACTGGG 1 cut(s) 61
Bpu10I CCTNAGC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 627
BsaBI GATNNNNATC 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 129, 380
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 2 cut(s) 129, 380
BseGI GGATG 5 cut(s) 94, 125, 376, 391, 458
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 170, 255
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 6 cut(s) 119, 135, 232, 295, 335, 581
BsgI GTGCAG 1 cut(s) 342
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 535
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 2 cut(s) 271, 353
Bsp19I CCATGG 1 cut(s) 380
BspCNI CTCAG 2 cut(s) 171, 254
BspEI TCCGGA 1 cut(s) 197
BspPI GGATC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 535
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 129, 380
BssMI GATC 2 cut(s) 271, 353
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 3 cut(s) 16, 100, 565
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 2 cut(s) 113, 505
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 105, 328
BstDEI CTNAG 2 cut(s) 179, 241
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 5 cut(s) 94, 125, 376, 391, 458
BstKTI GATC 2 cut(s) 274, 356
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 2 cut(s) 271, 353
BstMWI GCNNNNNNNGC 3 cut(s) 113, 245, 505
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 525
BstSNI TACGTA 1 cut(s) 627
BstV1I GCAGC 6 cut(s) 119, 135, 232, 295, 335, 581
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 380
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 5 cut(s) 94, 125, 376, 391, 458
BtsIMutI CAGTG 2 cut(s) 49, 96
Cac8I GCNNGC 2 cut(s) 105, 328
CaiI CAGNNNCTG 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 5 cut(s) 157, 470, 481, 624, 641
CviAII CATG 2 cut(s) 381, 400
CviJI RGCY 7 cut(s) 80, 110, 245, 308, 326, 594, 608
CviKI_1 RGCY 7 cut(s) 80, 110, 245, 308, 326, 594, 608
CviQI GTAC 5 cut(s) 157, 470, 481, 624, 641
DdeI CTNAG 2 cut(s) 179, 241
DpnI GATC 2 cut(s) 273, 355
DpnII GATC 2 cut(s) 271, 353
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco105I TACGTA 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 380
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 525
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 2 cut(s) 384, 403
FaiI YATR 7 cut(s) 138, 165, 382, 401, 585, 622, 630
FatI CATG 2 cut(s) 380, 399
Fnu4HI GCNGC 6 cut(s) 108, 149, 246, 309, 324, 595
FokI GGATG 5 cut(s) 101, 112, 383, 398, 445
Fsp4HI GCNGC 6 cut(s) 108, 149, 246, 309, 324, 595
GluI GCNGC 6 cut(s) 108, 149, 246, 309, 324, 595
HapII CCGG 1 cut(s) 198
Hin1II CATG 2 cut(s) 384, 403
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 208, 293
Hpy166II GTNNAC 2 cut(s) 58, 538
Hpy188I TCNGA 5 cut(s) 40, 133, 180, 366, 435
Hpy188III TCNNGA 2 cut(s) 198, 314
Hpy8I GTNNAC 2 cut(s) 58, 538
HpyAV CCTTC 4 cut(s) 197, 298, 434, 619
HpyCH4III ACNGT 3 cut(s) 16, 100, 565
HpyCH4IV ACGT 2 cut(s) 319, 626
HpyCH4V TGCA 9 cut(s) 107, 116, 323, 330, 344, 403, 448, 499, 649
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 245, 505
HpyF3I CTNAG 2 cut(s) 179, 241
HpySE526I ACGT 2 cut(s) 319, 626
Hsp92II CATG 2 cut(s) 384, 403
Kpn2I TCCGGA 1 cut(s) 197
Kzo9I GATC 2 cut(s) 271, 353
LmnI GCTCC 2 cut(s) 238, 455
LpnPI CCDG 9 cut(s) 37, 62, 174, 211, 249, 340, 486, 512, 539
Lsp1109I GCAGC 6 cut(s) 119, 135, 232, 295, 335, 581
LweI GCATC 4 cut(s) 220, 339, 347, 467
MabI ACCWGGT 1 cut(s) 525
MaeII ACGT 2 cut(s) 319, 626
MaeIII GTNAC 2 cut(s) 42, 214
MalI GATC 2 cut(s) 273, 355
MboI GATC 2 cut(s) 271, 353
MboII GAAGA 5 cut(s) 263, 266, 296, 305, 313
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 1 cut(s) 271
MluCI AATT 3 cut(s) 19, 513, 573
MnlI CCTC 6 cut(s) 139, 163, 174, 250, 289, 544
MroI TCCGGA 1 cut(s) 197
MseI TTAA 2 cut(s) 18, 576
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 527
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 3 cut(s) 113, 245, 505
NcoI CCATGG 1 cut(s) 380
NdeII GATC 2 cut(s) 271, 353
NlaIII CATG 2 cut(s) 384, 403
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 1 cut(s) 403
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 6 cut(s) 109, 150, 247, 310, 325, 596
Ppu21I YACGTR 1 cut(s) 627
PshBI ATTAAT 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PsrI GAACNNNNNNTAC 2 cut(s) 414, 446
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 271
PsyI GACNNNGTC 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 5 cut(s) 158, 471, 482, 625, 642
RsaNI GTAC 5 cut(s) 157, 470, 481, 624, 641
SaqAI TTAA 2 cut(s) 18, 576
SatI GCNGC 6 cut(s) 108, 149, 246, 309, 324, 595
Sau3AI GATC 2 cut(s) 271, 353
ScrFI CCNGG 1 cut(s) 527
SetI ASST 9 cut(s) 112, 208, 247, 322, 445, 475, 528, 596, 629
SexAI ACCWGGT 1 cut(s) 525
SfaNI GCATC 4 cut(s) 220, 339, 347, 467
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SnaBI TACGTA 1 cut(s) 627
Sse9I AATT 3 cut(s) 19, 513, 573
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 3 cut(s) 16, 100, 565
TaiI ACGT 2 cut(s) 322, 629
TasI AATT 3 cut(s) 19, 513, 573
TatI WGTACW 1 cut(s) 640
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 18, 576
Tru9I TTAA 2 cut(s) 18, 576
TscAI CASTG 2 cut(s) 56, 103
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 6 cut(s) 107, 148, 245, 308, 323, 594
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 2 cut(s) 56, 103
Tth111I GACNNNGTC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 576
XceI RCATGY 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.