RchiOBHm_Chr7g0190391

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003320
Physical Location & Seq
Reverse (-)
9475908 .. 9477169
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17004

Sequence Viewer

Length: 555 bp
ATGGAATCCTTGACTGTTAATTTGTTCCTTACTTTCTCGGAGTTACATCACTGGTTTGAGGAAACTAGAAGGATTTCAGGGGATGTTTATAGACTTGAATTTGCAACATTGTTGCTTTTCACGTATCAGGCTTCAAAGGATGATATCACTGTGCTTGCAGCTCTTGCAAAACCCCCATCCTCGGAATATGTGAATGTGCTGCGATGGTACAAACATATTGAGGCACTTCTGAGGATTTCTGGTGTTTCCGGAGAAGGTTGTGGTGTCACCATCAAGGGAGATGCTCCTGCCTCAGCTGCTGAAGAAGATGATGATGATGTGGATCTTTTTGGTGAAGAAACCGAAGAGGAGAAGAAGGCTGCTCAAGAACGTGCAGCCTGCGTCAAGGCATCTGCAAAAAAGAAAGAGTCCGGCAAGTCGTCAGTTCTGATGGATGTCAAACCATGGGATGATGAAACCGACATGCAAAAACTTTTGGAAGCAGTAAAAAATGTTCAGATGGAAGGTTTGCATTGGGGAGCATCCAAACTTGTACCTGTTGGGTACGGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

184

Amino Acids

20.43

Weight (kDa)

4.8

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 141 - 184 2.2e-15 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 248
AclWI GGATC 1 cut(s) 330
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 3 cut(s) 209, 534, 545
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 2 cut(s) 98, 135
AluBI AGCT 2 cut(s) 161, 296
AluI AGCT 2 cut(s) 161, 296
AlwI GGATC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 248
ApeKI GCWGC 5 cut(s) 158, 199, 296, 359, 374
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 259, 344
BbvCI CCTCAGC 1 cut(s) 292
BbvI GCAGC 5 cut(s) 170, 186, 283, 346, 386
BccI CCATC 5 cut(s) 184, 198, 278, 424, 493
BfaI CTAG 1 cut(s) 66
BisI GCNGC 5 cut(s) 159, 200, 297, 360, 375
BlsI GCNGC 5 cut(s) 160, 201, 298, 361, 376
BmsI GCATC 3 cut(s) 271, 398, 530
Bpu10I CCTNAGC 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 180, 443
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 248
BseDI CCNNGG 2 cut(s) 180, 443
BseGI GGATG 6 cut(s) 88, 145, 176, 439, 454, 521
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 221, 306
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 5 cut(s) 170, 186, 283, 346, 386
BsgI GTGCAG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 249, 411
BslI CCNNNNNNNGG 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 248
Bsp143I GATC 1 cut(s) 322
Bsp19I CCATGG 1 cut(s) 443
BspCNI CTCAG 2 cut(s) 222, 305
BspEI TCCGGA 1 cut(s) 248
BspPI GGATC 1 cut(s) 330
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 180, 443
BssMI GATC 1 cut(s) 322
BssT1I CCWWGG 1 cut(s) 443
Bst4CI ACNGT 2 cut(s) 16, 151
Bst6I CTCTTC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 156, 379
BstDEI CTNAG 2 cut(s) 230, 292
BstDSI CCRYGG 1 cut(s) 443
BstF5I GGATG 6 cut(s) 88, 145, 176, 439, 454, 521
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 2 cut(s) 164, 296
BstNSI RCATGY 1 cut(s) 466
BstV1I GCAGC 5 cut(s) 170, 186, 283, 346, 386
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 443
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 6 cut(s) 88, 145, 176, 439, 454, 521
BtsIMutI CAGTG 2 cut(s) 49, 147
Cac8I GCNNGC 2 cut(s) 156, 379
CaiI CAGNNNCTG 1 cut(s) 299
CseI GACGC 1 cut(s) 370
Csp6I GTAC 3 cut(s) 208, 533, 544
CviAII CATG 2 cut(s) 444, 463
CviJI RGCY 5 cut(s) 131, 161, 296, 359, 377
CviKI_1 RGCY 5 cut(s) 131, 161, 296, 359, 377
CviQI GTAC 3 cut(s) 208, 533, 544
DdeI CTNAG 2 cut(s) 230, 292
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 443
Eco32I GATATC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 321
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 2 cut(s) 447, 466
FaiI YATR 6 cut(s) 90, 189, 216, 445, 464, 553
FatI CATG 2 cut(s) 443, 462
Fnu4HI GCNGC 5 cut(s) 159, 200, 297, 360, 375
FokI GGATG 6 cut(s) 95, 152, 163, 446, 461, 508
Fsp4HI GCNGC 5 cut(s) 159, 200, 297, 360, 375
FspBI CTAG 1 cut(s) 66
GluI GCNGC 5 cut(s) 159, 200, 297, 360, 375
HapII CCGG 2 cut(s) 249, 411
HgaI GACGC 1 cut(s) 370
Hin1II CATG 2 cut(s) 447, 466
HinfI GANTC 2 cut(s) 5, 407
HpaII CCGG 2 cut(s) 249, 411
HphI GGTGA 2 cut(s) 259, 344
Hpy188I TCNGA 5 cut(s) 40, 184, 231, 429, 498
Hpy188III TCNNGA 2 cut(s) 249, 365
HpyAV CCTTC 4 cut(s) 63, 248, 349, 497
HpyCH4III ACNGT 2 cut(s) 16, 151
HpyCH4IV ACGT 2 cut(s) 122, 370
HpyCH4V TGCA 7 cut(s) 104, 158, 167, 374, 395, 466, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 296
HpyF3I CTNAG 2 cut(s) 230, 292
HpySE526I ACGT 2 cut(s) 122, 370
Hsp92II CATG 2 cut(s) 447, 466
Kpn2I TCCGGA 1 cut(s) 248
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 2 cut(s) 289, 518
LpnPI CCDG 9 cut(s) 37, 63, 113, 225, 262, 300, 391, 424, 549
Lsp1109I GCAGC 5 cut(s) 170, 186, 283, 346, 386
LweI GCATC 3 cut(s) 271, 398, 530
MaeI CTAG 1 cut(s) 66
MaeII ACGT 2 cut(s) 122, 370
MaeIII GTNAC 2 cut(s) 42, 265
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 5 cut(s) 314, 317, 347, 356, 364
MflI RGATCY 1 cut(s) 322
MluCI AATT 2 cut(s) 19, 98
MlyI GAGTC 1 cut(s) 416
MnlI CCTC 6 cut(s) 52, 190, 214, 225, 301, 340
MroI TCCGGA 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 249, 411
MwoI GCNNNNNNNGC 2 cut(s) 164, 296
NcoI CCATGG 1 cut(s) 443
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 447, 466
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 5 cut(s) 160, 201, 298, 361, 376
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Ppu21I YACGTR 1 cut(s) 123
PsrI GAACNNNNNNTAC 2 cut(s) 477, 509
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 3 cut(s) 209, 534, 545
RsaNI GTAC 3 cut(s) 208, 533, 544
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 5 cut(s) 159, 200, 297, 360, 375
Sau3AI GATC 1 cut(s) 322
SchI GAGTC 1 cut(s) 416
SetI ASST 7 cut(s) 125, 163, 259, 298, 373, 508, 538
SfaNI GCATC 3 cut(s) 271, 398, 530
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
Sse9I AATT 2 cut(s) 19, 98
SspMI CTAG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 2 cut(s) 16, 151
TaiI ACGT 2 cut(s) 125, 373
TasI AATT 2 cut(s) 19, 98
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 56, 154
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 5 cut(s) 158, 199, 296, 359, 374
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 468
TspRI CASTG 2 cut(s) 56, 154
XapI RAATTY 1 cut(s) 98
XceI RCATGY 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.