MD06G1013800.v1.1

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003320
Physical Location & Seq
Reverse (-)
1741186 .. 1741741
556 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1013800.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCAGTCACATTCTTTGGCCTCAACTCGGCTGCTGGCCTGAAAAAACTGGATGACTACTTGCTTGATCACAGTTACATCACTGGCTCTCAGGCTTCCAAGGATGATCTCACTGTGCATGCAGCTCTTTCTAAGCCACCATCTCCGGAATTTGTGAATGTGTCTAGGTGGTACAACCACATCGCTGCTCTTTTTTTCTGCTGTTTTTGGAGAAGGATCTGGTCTGAAACAAAGGCAGCTGACGATGTTGATCTCTTTGGCGAAGAGTTCGTCGATGTTGATCTCGAAGAGACCAAAGGGGAGAAGAAGGTTGCTGAAGAACGTGTGGCGGCTTTCGTCAAGGCATATACAGAGAAGAAAGAGTATGGAAAGTCATCAGTTCTGTTGGACGTGAAGCCATGGGATGATGAAACTGACATGAAACTGCGTGAGGAGGCTGTAAGAAGTGTCGACATGGAAGGGATGCATTGGGGAACATCCAAACTTGTAGATGTTGGGTACGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

169

Amino Acids

19.03

Weight (kDa)

4.87

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 126 - 168 9.8e-12 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 145
AciI CCGC 1 cut(s) 329
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 2 cut(s) 173, 500
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 322
AjiI CACGTC 1 cut(s) 391
AluBI AGCT 2 cut(s) 125, 239
AluI AGCT 2 cut(s) 125, 239
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 1 cut(s) 224
Aor13HI TCCGGA 1 cut(s) 145
AoxI GGCC 2 cut(s) 19, 37
ApeKI GCWGC 4 cut(s) 32, 122, 185, 236
ApoI RAATTY 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 255, 287
BbvI GCAGC 4 cut(s) 19, 134, 172, 248
BccI CCATC 1 cut(s) 148
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 165
BisI GCNGC 5 cut(s) 33, 123, 186, 237, 330
BlsI GCNGC 5 cut(s) 34, 124, 187, 238, 331
BmgBI CACGTC 1 cut(s) 391
BmsI GCATC 1 cut(s) 453
BsaBI GATNNNNATC 2 cut(s) 249, 279
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 99, 398
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 54, 88
Bse8I GATNNNNATC 2 cut(s) 249, 279
BseAI TCCGGA 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 99, 398
BseGI GGATG 5 cut(s) 58, 109, 409, 468, 476
BseJI GATNNNNATC 2 cut(s) 249, 279
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 54, 88
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 4 cut(s) 19, 134, 172, 248
BshFI GGCC 2 cut(s) 21, 39
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 2 cut(s) 21, 39
Bso31I GGTCTC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 145
Bsp143I GATC 5 cut(s) 67, 106, 216, 250, 280
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 1 cut(s) 329
BspANI GGCC 2 cut(s) 21, 39
BspCNI CTCAG 1 cut(s) 103
BspEI TCCGGA 1 cut(s) 145
BspPI GGATC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 284
BsrI ACTGG 2 cut(s) 54, 88
BssECI CCNNGG 2 cut(s) 99, 398
BssMI GATC 5 cut(s) 67, 106, 216, 250, 280
BssT1I CCWWGG 2 cut(s) 99, 398
Bst4CI ACNGT 2 cut(s) 74, 115
Bst6I CTCTTC 2 cut(s) 258, 282
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 2 cut(s) 90, 132
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 5 cut(s) 58, 109, 409, 468, 476
BstKTI GATC 5 cut(s) 70, 109, 219, 253, 283
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 5 cut(s) 67, 106, 216, 250, 280
BstNSI RCATGY 1 cut(s) 122
BstV1I GCAGC 4 cut(s) 19, 134, 172, 248
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 2 cut(s) 21, 39
BtgI CCRYGG 1 cut(s) 398
BtgZI GCGATG 1 cut(s) 166
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 5 cut(s) 58, 109, 409, 468, 476
BtsIMutI CAGTG 2 cut(s) 81, 111
Cac8I GCNNGC 2 cut(s) 37, 120
Csp6I GTAC 2 cut(s) 172, 499
CviAII CATG 4 cut(s) 119, 399, 418, 454
CviQI GTAC 2 cut(s) 172, 499
DdeI CTNAG 2 cut(s) 90, 132
DpnI GATC 5 cut(s) 69, 108, 218, 252, 282
DpnII GATC 5 cut(s) 67, 106, 216, 250, 280
Eam1104I CTCTTC 2 cut(s) 258, 282
EarI CTCTTC 2 cut(s) 258, 282
Eco130I CCWWGG 2 cut(s) 99, 398
Eco31I GGTCTC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 99, 398
EcoT22I ATGCAT 1 cut(s) 468
ErhI CCWWGG 2 cut(s) 99, 398
FaeI CATG 4 cut(s) 122, 402, 421, 457
FaiI YATR 7 cut(s) 120, 346, 348, 366, 400, 419, 455
FatI CATG 4 cut(s) 118, 398, 417, 453
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 5 cut(s) 33, 123, 186, 237, 330
FokI GGATG 5 cut(s) 65, 116, 416, 463, 475
Fsp4HI GCNGC 5 cut(s) 33, 123, 186, 237, 330
FspBI CTAG 1 cut(s) 165
GluI GCNGC 5 cut(s) 33, 123, 186, 237, 330
HaeIII GGCC 2 cut(s) 21, 39
HapII CCGG 1 cut(s) 146
Hin1II CATG 4 cut(s) 122, 402, 421, 457
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HpaII CCGG 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 2 cut(s) 146, 284
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 207, 301, 452
HpyCH4III ACNGT 2 cut(s) 74, 115
HpyCH4IV ACGT 2 cut(s) 322, 390
HpyCH4V TGCA 3 cut(s) 118, 122, 466
HpyF3I CTNAG 2 cut(s) 90, 132
HpySE526I ACGT 2 cut(s) 322, 390
Hsp92II CATG 4 cut(s) 122, 402, 421, 457
Kpn2I TCCGGA 1 cut(s) 145
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 106, 216, 250, 280
LpnPI CCDG 7 cut(s) 21, 35, 53, 69, 77, 159, 205
Lsp1109I GCAGC 4 cut(s) 19, 134, 172, 248
LweI GCATC 1 cut(s) 453
MaeI CTAG 1 cut(s) 165
MaeII ACGT 2 cut(s) 322, 390
MaeIII GTNAC 2 cut(s) 7, 74
MalI GATC 5 cut(s) 69, 108, 218, 252, 282
MboI GATC 5 cut(s) 67, 106, 216, 250, 280
MboII GAAGA 5 cut(s) 275, 299, 316, 329, 367
MflI RGATCY 1 cut(s) 216
MluCI AATT 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 3 cut(s) 32, 424, 427
Mph1103I ATGCAT 1 cut(s) 468
MroI TCCGGA 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 398
NdeII GATC 5 cut(s) 67, 106, 216, 250, 280
NlaIII CATG 4 cut(s) 122, 402, 421, 457
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 1 cut(s) 7
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PkrI GCNGC 5 cut(s) 34, 124, 187, 238, 331
PsuI RGATCY 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 173, 500
RsaNI GTAC 2 cut(s) 172, 499
SalI GTCGAC 1 cut(s) 449
SatI GCNGC 5 cut(s) 33, 123, 186, 237, 330
Sau3AI GATC 5 cut(s) 67, 106, 216, 250, 280
SetI ASST 6 cut(s) 127, 170, 241, 312, 325, 393
SfaNI GCATC 1 cut(s) 453
SphI GCATGC 1 cut(s) 122
Sse9I AATT 1 cut(s) 149
SsiI CCGC 1 cut(s) 329
SspMI CTAG 1 cut(s) 165
StyI CCWWGG 2 cut(s) 99, 398
TaaI ACNGT 2 cut(s) 74, 115
TaiI ACGT 2 cut(s) 325, 393
TaqI TCGA 3 cut(s) 273, 285, 450
TasI AATT 1 cut(s) 149
TauI GCSGC 1 cut(s) 332
TscAI CASTG 2 cut(s) 88, 118
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 32, 122, 185, 236
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 423, 434
TspRI CASTG 2 cut(s) 88, 118
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.