RLG00000030760

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003320
Physical Location & Seq
Reverse (-)
66999138 .. 67000915
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030760

Sequence Viewer

Length: 813 bp
ATGGCAGTTGCATTCCATGACATCAACTCGGCTGCTGGCCTCAAGAAACTGGACCAGTACTTGCTCACTCGCAGTTACATCACCGGGTATCAGGCTTCAAAGGATGATGTCACTGTGCATGCTGCTCTGTCTAAGCCTCCATCAGCTGAATACGTGAATGCATCTCGGTGGTACAACCACATTGCTGCACTTCTTAGGATTTCGGGTGTTTCTGGAGAAGGTTGTGGTGTCACAATTGAGGGATCTGCTCCCATCACAGCAGAAGCAGTTGCCACACCTCCAGCTGGTGATTCAAAGGCCTCTGCTGCTGAAGATGATGATGATAGTGATGTGGATCTCTTTGGTGAAGAGACTGAAGAGGAGAAGAAGGCTGCCGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAAGAGTCTGGCAAGTCATCAGTTCTATTGGATATTAAGCCATGGGATGATGAAACAGACATGAAAAAGCTTGAGGAGGCAGTAAGGAGCATTGAGGTTGAGGGCTTACATTGGGGAGCATCTAAACTAGTACCTGTTGGGTATGGCATAAAGAAGTTGACAATTATGCTTACAATTGTTGATGACCTTGTTTCTGTGGACTCTCTGATTGAAGAACGTCTTACCGTGGAACCAATTAATGAATATGTCCAGAGCTGTGACATTGTTGCCTTCAATAAAATATTTTCCAGGCTGGTTAACGGCCAATGCAATTTAGGCTTTGTTGATCAGTGTAGGTTCTTATTTTTGTCTGAGTTGAAACAAGAACAAATGTCAGGCTCAATTGCTACCCTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

271

Amino Acids

29.29

Weight (kDa)

4.76

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 145 - 232 2.5e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 250, 342
AcoI YGGCCR 1 cut(s) 715
AcuI CTGAAG 2 cut(s) 330, 375
AfaI GTAC 3 cut(s) 59, 173, 546
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 5 cut(s) 99, 294, 626, 688, 772
AhlI ACTAGT 1 cut(s) 541
AjnI CCWGG 1 cut(s) 701
AluBI AGCT 5 cut(s) 146, 284, 389, 484, 669
AluI AGCT 5 cut(s) 146, 284, 389, 484, 669
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 2 cut(s) 250, 342
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 3 cut(s) 37, 297, 715
ApeKI GCWGC 7 cut(s) 32, 122, 185, 305, 371, 386, 389
AseI ATTAAT 1 cut(s) 651
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 73, 299, 356
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BccI CCATC 2 cut(s) 148, 260
BceAI ACGGC 1 cut(s) 730
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 703
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 344
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 1 cut(s) 542
BisI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
BlsI GCNGC 7 cut(s) 34, 124, 187, 307, 373, 388, 391
BmcAI AGTACT 1 cut(s) 59
Bme1390I CCNGG 2 cut(s) 85, 703
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 2 cut(s) 85, 703
BmsI GCATC 3 cut(s) 170, 410, 542
BpmI CTGGAG 2 cut(s) 234, 264
BpuEI CTTGAG 2 cut(s) 26, 506
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 455, 639
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 54, 55
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 703
BseDI CCNNGG 2 cut(s) 455, 639
BseGI GGATG 2 cut(s) 109, 466
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 2 cut(s) 54, 55
BseRI GAGGAG 2 cut(s) 374, 503
BseXI GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BsgI GTGCAG 2 cut(s) 171, 405
BshFI GGCC 3 cut(s) 39, 299, 717
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 2 cut(s) 11, 163
BsnI GGCC 3 cut(s) 39, 299, 717
Bsp143I GATC 3 cut(s) 242, 334, 739
Bsp19I CCATGG 1 cut(s) 455
BspANI GGCC 3 cut(s) 39, 299, 717
BspCNI CTCAG 1 cut(s) 757
BspLI GGNNCC 1 cut(s) 645
BspPI GGATC 2 cut(s) 250, 342
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 2 cut(s) 54, 55
BssECI CCNNGG 2 cut(s) 455, 639
BssMI GATC 3 cut(s) 242, 334, 739
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 1 cut(s) 703
Bst4CI ACNGT 2 cut(s) 115, 640
Bst6I CTCTTC 2 cut(s) 342, 351
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 3 cut(s) 132, 194, 765
BstDSI CCRYGG 2 cut(s) 455, 639
BstF5I GGATG 2 cut(s) 109, 466
BstKTI GATC 3 cut(s) 245, 337, 742
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 3 cut(s) 242, 334, 739
BstMWI GCNNNNNNNGC 3 cut(s) 305, 398, 729
BstNI CCWGG 1 cut(s) 703
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 83, 701
BstV1I GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
BstX2I RGATCY 2 cut(s) 242, 334
BstYI RGATCY 2 cut(s) 242, 334
BsuRI GGCC 3 cut(s) 39, 299, 717
BtgI CCRYGG 2 cut(s) 455, 639
BtsCI GGATG 2 cut(s) 109, 466
BtsIMutI CAGTG 2 cut(s) 111, 749
Cac8I GCNNGC 2 cut(s) 37, 120
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 3 cut(s) 58, 172, 545
CviAII CATG 4 cut(s) 17, 119, 456, 475
CviQI GTAC 3 cut(s) 58, 172, 545
DdeI CTNAG 3 cut(s) 132, 194, 765
DpnI GATC 3 cut(s) 244, 336, 741
DpnII GATC 3 cut(s) 242, 334, 739
EaeI YGGCCR 1 cut(s) 715
Eam1104I CTCTTC 2 cut(s) 342, 351
EarI CTCTTC 2 cut(s) 342, 351
Eco130I CCWWGG 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 330, 375
EcoRII CCWGG 1 cut(s) 701
EcoT14I CCWWGG 1 cut(s) 455
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 4 cut(s) 20, 122, 459, 478
FaiI YATR 8 cut(s) 18, 120, 457, 476, 558, 563, 581, 660
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 4 cut(s) 16, 118, 455, 474
FbaI TGATCA 1 cut(s) 739
Fnu4HI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
FokI GGATG 2 cut(s) 116, 473
Fsp4HI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
FspBI CTAG 1 cut(s) 542
GluI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
GsuI CTGGAG 2 cut(s) 234, 264
HaeIII GGCC 3 cut(s) 39, 299, 717
HapII CCGG 1 cut(s) 84
Hin1II CATG 4 cut(s) 20, 122, 459, 478
HincII GTYRAC 2 cut(s) 573, 712
HindII GTYRAC 2 cut(s) 573, 712
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 3 cut(s) 290, 419, 614
HpaI GTTAAC 1 cut(s) 712
HpaII CCGG 1 cut(s) 84
HphI GGTGA 3 cut(s) 73, 299, 356
Hpy166II GTNNAC 3 cut(s) 573, 613, 712
Hpy188I TCNGA 2 cut(s) 621, 766
Hpy188III TCNNGA 3 cut(s) 43, 213, 664
Hpy8I GTNNAC 3 cut(s) 573, 613, 712
HpyAV CCTTC 3 cut(s) 212, 361, 694
HpyCH4III ACNGT 2 cut(s) 115, 640
HpyCH4IV ACGT 3 cut(s) 153, 382, 631
HpyCH4V TGCA 6 cut(s) 11, 118, 161, 188, 386, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 398, 729
HpyF3I CTNAG 3 cut(s) 132, 194, 765
HpySE526I ACGT 3 cut(s) 153, 382, 631
Hsp92II CATG 4 cut(s) 20, 122, 459, 478
Ksp22I TGATCA 1 cut(s) 739
KspAI GTTAAC 1 cut(s) 712
Kzo9I GATC 3 cut(s) 242, 334, 739
LmnI GCTCC 3 cut(s) 253, 501, 530
Lsp1109I GCAGC 7 cut(s) 19, 109, 172, 292, 358, 376, 398
LweI GCATC 3 cut(s) 170, 410, 542
MaeI CTAG 1 cut(s) 542
MaeII ACGT 3 cut(s) 153, 382, 631
MaeIII GTNAC 4 cut(s) 74, 109, 229, 671
MalI GATC 3 cut(s) 244, 336, 741
MboI GATC 3 cut(s) 242, 334, 739
MboII GAAGA 6 cut(s) 323, 359, 368, 376, 389, 638
MfeI CAATTG 3 cut(s) 234, 588, 795
MflI RGATCY 2 cut(s) 242, 334
MluCI AATT 6 cut(s) 234, 576, 588, 648, 724, 795
MlyI GAGTC 2 cut(s) 428, 608
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 3 cut(s) 450, 651, 711
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 3 cut(s) 146, 284, 389
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 2 cut(s) 85, 703
MunI CAATTG 3 cut(s) 234, 588, 795
Mva1269I GAATGC 2 cut(s) 11, 163
MvaI CCWGG 1 cut(s) 703
MwoI GCNNNNNNNGC 3 cut(s) 305, 398, 729
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 1 cut(s) 455
NdeII GATC 3 cut(s) 242, 334, 739
NlaIII CATG 4 cut(s) 20, 122, 459, 478
NlaIV GGNNCC 1 cut(s) 645
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 3 cut(s) 109, 229, 671
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 2 cut(s) 11, 163
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 7 cut(s) 34, 124, 187, 307, 373, 388, 391
PleI GAGTC 2 cut(s) 427, 608
PpsI GAGTC 2 cut(s) 427, 608
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 701
PspGI CCWGG 1 cut(s) 701
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 242, 334
PvuII CAGCTG 3 cut(s) 146, 284, 389
RsaI GTAC 3 cut(s) 59, 173, 546
RsaNI GTAC 3 cut(s) 58, 172, 545
RseI CAYNNNNRTG 1 cut(s) 166
SaqAI TTAA 3 cut(s) 450, 651, 711
SatI GCNGC 7 cut(s) 33, 123, 186, 306, 372, 387, 390
Sau3AI GATC 3 cut(s) 242, 334, 739
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 2 cut(s) 428, 608
ScrFI CCNGG 2 cut(s) 85, 703
SfaNI GCATC 3 cut(s) 170, 410, 542
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 41, 485
SmoI CTYRAG 2 cut(s) 41, 485
SpeI ACTAGT 1 cut(s) 541
SphI GCATGC 1 cut(s) 122
Sse9I AATT 6 cut(s) 234, 576, 588, 648, 724, 795
SseBI AGGCCT 1 cut(s) 299
SspI AATATT 1 cut(s) 696
SspMI CTAG 1 cut(s) 542
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 83, 701
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 115, 640
TaiI ACGT 3 cut(s) 156, 385, 634
TasI AATT 6 cut(s) 234, 576, 588, 648, 724, 795
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 3 cut(s) 450, 651, 711
Tru9I TTAA 3 cut(s) 450, 651, 711
TscAI CASTG 2 cut(s) 118, 749
TseFI GTSAC 3 cut(s) 109, 229, 671
TseI GCWGC 7 cut(s) 32, 122, 185, 305, 371, 386, 389
Tsp45I GTSAC 3 cut(s) 109, 229, 671
TspDTI ATGAA 4 cut(s) 480, 491, 669, 797
TspRI CASTG 2 cut(s) 118, 749
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 651
XceI RCATGY 1 cut(s) 122
XspI CTAG 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 59
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.