MD07G1005900.v1.1

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003320
Physical Location & Seq
Forward (+)
582660 .. 584691
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1005900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCAGTCACATTCTTTGACGTGAACTCAGCTGCCGGCCTGAAGAAACTTGATGACTACTTGCTTTCTCGCAGTTACATCACTGGCTATCAGGCTTCCAAAGATGATATCACTGTGCATGCAGCTCTTTCCAAGGCCCCGTCTTCGGAATTTGTGAATGTGTCTAGGTGGTACAACCACATTGCTGCTCTTCTTAGGATTTCTGGTGTTTGCGGAGAAGGATCTGGTGTCACTATTGAGGGATCTGCTCCCGTAACCAGGGAAGCAGTTGCTACTCCTCCTGTTGCTGACACTAAGGCCTCAGCTGCTGAGGATGACGATGACGATGTTGATCTTTTTGGTGAAGAGACCGAAGAGGAGAAGAAGGCTGCTGAAGAGCGTGCGGCTTCCATCAAGGCATCTACAAAGAAGAAAGAGTCTGGCAAGTCATCGGTTCTGTTGGATGTGAAGCCATGGGATGATGAAACTGACATGAAAAAGCTTGAGGAGGCTGTAAGAAGTGTCCACATGGATGGCTTGCTTTGGGGAGCATCCAAACTCGTAGCTGTTGGGTACGGGATTAAGAAGTTGCAAATAATGATGACAATTGTTGATGACCTGGTCTCTGTTGATACTCTCATTGAGGAGCAACTTACTGTTGAACCAATTAACGAGTATGTCCAGAGTTGCGACATCGTAGCCTTCAACAAGATATGTAAGATTGGAGCTTGCGGATGTGTATTTTGTTTCTGGCGGTATGATTTGAGACATTATATTTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

255

Amino Acids

27.92

Weight (kDa)

4.76

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 144 - 231 8.5e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 213, 383, 711, 733
AclWI GGATC 2 cut(s) 229, 250
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 2 cut(s) 62, 393
AfaI GTAC 2 cut(s) 173, 554
AfiI CCNNNNNNNGG 2 cut(s) 145, 258
AgsI TTSAA 2 cut(s) 641, 685
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 257, 597
AluBI AGCT 6 cut(s) 32, 125, 305, 481, 545, 707
AluI AGCT 6 cut(s) 32, 125, 305, 481, 545, 707
Alw26I GTCTC 3 cut(s) 341, 607, 739
AlwI GGATC 2 cut(s) 229, 250
AlwNI CAGNNNCTG 1 cut(s) 308
AoxI GGCC 3 cut(s) 37, 135, 297
ApeKI GCWGC 5 cut(s) 32, 122, 185, 305, 368
ApoI RAATTY 1 cut(s) 149
ArsI GACNNNNNNTTYG 1 cut(s) 738
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 135
BbvCI CCTCAGC 2 cut(s) 301, 309
BbvI GCAGC 5 cut(s) 19, 134, 172, 292, 355
BccI CCATC 2 cut(s) 398, 506
BciT130I CCWGG 2 cut(s) 259, 599
BcoDI GTCTC 3 cut(s) 341, 607, 739
BfaI CTAG 1 cut(s) 165
BisI GCNGC 6 cut(s) 33, 123, 186, 306, 369, 384
BlsI GCNGC 6 cut(s) 34, 124, 187, 307, 370, 385
Bme1390I CCNGG 2 cut(s) 259, 599
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 259, 599
BmsI GCATC 2 cut(s) 407, 539
BpiI GAAGAC 1 cut(s) 135
Bpu10I CCTNAGC 2 cut(s) 301, 309
BpuEI CTTGAG 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 2 cut(s) 341, 607
BsaJI CCNNGG 3 cut(s) 132, 258, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 258
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 2 cut(s) 259, 599
BseDI CCNNGG 3 cut(s) 132, 258, 452
BseGI GGATG 6 cut(s) 319, 448, 463, 517, 530, 719
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 145, 258
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 3 cut(s) 42, 300, 315
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 4 cut(s) 267, 371, 500, 638
BseXI GCAGC 5 cut(s) 19, 134, 172, 292, 355
BshFI GGCC 3 cut(s) 39, 137, 299
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 145, 258
BsmAI GTCTC 3 cut(s) 341, 607, 739
BsnI GGCC 3 cut(s) 39, 137, 299
Bso31I GGTCTC 2 cut(s) 341, 607
Bsp143I GATC 3 cut(s) 221, 242, 331
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 4 cut(s) 213, 383, 711, 733
BspANI GGCC 3 cut(s) 39, 137, 299
BspCNI CTCAG 3 cut(s) 41, 301, 314
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 2 cut(s) 229, 250
BspQI GCTCTTC 2 cut(s) 195, 369
BspTNI GGTCTC 2 cut(s) 341, 607
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 132, 258, 452
BssMI GATC 3 cut(s) 221, 242, 331
BssT1I CCWWGG 2 cut(s) 132, 452
Bst2UI CCWGG 2 cut(s) 259, 599
Bst4CI ACNGT 2 cut(s) 115, 637
Bst6I CTCTTC 4 cut(s) 195, 339, 348, 369
BstC8I GCNNGC 5 cut(s) 37, 120, 381, 518, 709
BstDEI CTNAG 5 cut(s) 28, 194, 294, 301, 309
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 6 cut(s) 319, 448, 463, 517, 530, 719
BstKTI GATC 3 cut(s) 224, 245, 334
BstMAI GTCTC 3 cut(s) 341, 607, 739
BstMBI GATC 3 cut(s) 221, 242, 331
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 2 cut(s) 259, 599
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 257, 597
BstV1I GCAGC 5 cut(s) 19, 134, 172, 292, 355
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 2 cut(s) 221, 242
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 2 cut(s) 221, 242
BsuRI GGCC 3 cut(s) 39, 137, 299
BtgI CCRYGG 1 cut(s) 452
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 6 cut(s) 319, 448, 463, 517, 530, 719
BtsIMutI CAGTG 2 cut(s) 81, 111
Cac8I GCNNGC 5 cut(s) 37, 120, 381, 518, 709
CaiI CAGNNNCTG 1 cut(s) 308
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 597
Csp6I GTAC 2 cut(s) 172, 553
CviAII CATG 4 cut(s) 119, 453, 472, 508
CviQI GTAC 2 cut(s) 172, 553
DdeI CTNAG 5 cut(s) 28, 194, 294, 301, 309
DpnI GATC 3 cut(s) 223, 244, 333
DpnII GATC 3 cut(s) 221, 242, 331
Eam1104I CTCTTC 4 cut(s) 195, 339, 348, 369
EarI CTCTTC 4 cut(s) 195, 339, 348, 369
Eco130I CCWWGG 2 cut(s) 132, 452
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 2 cut(s) 341, 607
Eco32I GATATC 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 62, 393
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 257, 597
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 132, 452
ErhI CCWWGG 2 cut(s) 132, 452
FaeI CATG 4 cut(s) 122, 456, 475, 511
FaiI YATR 8 cut(s) 120, 454, 473, 509, 657, 694, 738, 753
FatI CATG 4 cut(s) 118, 452, 471, 507
Fnu4HI GCNGC 6 cut(s) 33, 123, 186, 306, 369, 384
FokI GGATG 6 cut(s) 326, 455, 470, 517, 524, 726
Fsp4HI GCNGC 6 cut(s) 33, 123, 186, 306, 369, 384
FspBI CTAG 1 cut(s) 165
GluI GCNGC 6 cut(s) 33, 123, 186, 306, 369, 384
HaeIII GGCC 3 cut(s) 39, 137, 299
HapII CCGG 1 cut(s) 36
Hin1II CATG 4 cut(s) 122, 456, 475, 511
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 1 cut(s) 416
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 25, 505
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 1 cut(s) 661
Hpy8I GTNNAC 2 cut(s) 25, 505
HpyAV CCTTC 3 cut(s) 212, 358, 691
HpyCH4III ACNGT 2 cut(s) 115, 637
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 118, 122, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 5 cut(s) 28, 194, 294, 301, 309
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 4 cut(s) 122, 456, 475, 511
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 221, 242, 331
LguI GCTCTTC 2 cut(s) 195, 369
LmnI GCTCC 4 cut(s) 253, 527, 625, 704
Lsp1109I GCAGC 5 cut(s) 19, 134, 172, 292, 355
LweI GCATC 2 cut(s) 407, 539
MabI ACCWGGT 1 cut(s) 597
MaeI CTAG 1 cut(s) 165
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 7, 74, 229, 253
MalI GATC 3 cut(s) 223, 244, 333
MboI GATC 3 cut(s) 221, 242, 331
MboII GAAGA 8 cut(s) 55, 135, 182, 356, 365, 373, 386, 421
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 2 cut(s) 221, 242
MluCI AATT 3 cut(s) 149, 585, 645
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 8 cut(s) 232, 288, 304, 310, 349, 478, 481, 616
MroNI GCCGGC 1 cut(s) 35
MseI TTAA 2 cut(s) 561, 648
MslI CAYNNNNRTG 1 cut(s) 510
MspA1I CMGCKG 2 cut(s) 32, 305
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 259, 599
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 2 cut(s) 259, 599
MwoI GCNNNNNNNGC 1 cut(s) 305
NaeI GCCGGC 1 cut(s) 37
NcoI CCATGG 1 cut(s) 452
NdeII GATC 3 cut(s) 221, 242, 331
NgoMIV GCCGGC 1 cut(s) 35
NlaIII CATG 4 cut(s) 122, 456, 475, 511
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 7, 229
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 299
PciSI GCTCTTC 2 cut(s) 195, 369
PdiI GCCGGC 1 cut(s) 37
PflFI GACNNNGTC 1 cut(s) 599
PkrI GCNGC 6 cut(s) 34, 124, 187, 307, 370, 385
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 257, 597
PspGI CCWGG 2 cut(s) 257, 597
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 2 cut(s) 221, 242
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 2 cut(s) 32, 305
RsaI GTAC 2 cut(s) 173, 554
RsaNI GTAC 2 cut(s) 172, 553
RseI CAYNNNNRTG 1 cut(s) 510
SapI GCTCTTC 2 cut(s) 195, 369
SaqAI TTAA 2 cut(s) 561, 648
SatI GCNGC 6 cut(s) 33, 123, 186, 306, 369, 384
Sau3AI GATC 3 cut(s) 221, 242, 331
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 2 cut(s) 259, 599
SetI ASST 9 cut(s) 24, 34, 127, 170, 307, 483, 547, 600, 709
SexAI ACCWGGT 1 cut(s) 597
SfaNI GCATC 2 cut(s) 407, 539
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SphI GCATGC 1 cut(s) 122
Sse9I AATT 3 cut(s) 149, 585, 645
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 4 cut(s) 213, 383, 711, 733
SspMI CTAG 1 cut(s) 165
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 257, 597
StyI CCWWGG 2 cut(s) 132, 452
TaaI ACNGT 2 cut(s) 115, 637
TaiI ACGT 1 cut(s) 24
TaqII GACCGA 1 cut(s) 365
TasI AATT 3 cut(s) 149, 585, 645
TauI GCSGC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 561, 648
Tru9I TTAA 2 cut(s) 561, 648
TscAI CASTG 2 cut(s) 88, 118
TseFI GTSAC 2 cut(s) 7, 229
TseI GCWGC 5 cut(s) 32, 122, 185, 305, 368
Tsp45I GTSAC 2 cut(s) 7, 229
TspDTI ATGAA 2 cut(s) 477, 488
TspRI CASTG 2 cut(s) 88, 118
Tth111I GACNNNGTC 1 cut(s) 599
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 122
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.