AT1G30230

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003320
Physical Location & Seq
Forward (+)
10638878 .. 10640941
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30230.2

Sequence Viewer

Length: 696 bp
ATGGCAGCATTCCCTAACCTTAACTCTGATGCTGGATTGAAGAAGCTCGACGAGCATCTTCTCACTCGCAGTTACATTACTGGATACCAGGCTTCAAAGGATGATATCACCGTCTTTGCAGCTCTTGCAAAGCCCCCAACTTCACAGTATGTGAACGCTTCTCGTTGGTACAATCACATTGATGCCCTCTTGAGGATCTCTGGTGTCTCTGCTGAAGGAAGCGGTGTCATTGTTGAGGGATCAGCTCCTATCACTGAGGAGGCTGTTGCTACTCCCCCTGCAGCTGATTCTAAGGATGCTGCGGCTGATGAAGAAGATGATGATGATGTTGACCTTTTCGGAGAGGAGACCGAAGAGGAAAAGAAAGCTGCTGAAGAGAGAGCAGCTTCTGTCAAGGCATCTACAAAGAAGAAGGAATCTGGAAAGTCCTCAGTTTTGATTGATATCAAACCGTGGGATGATGAGACTGACATGAAGAAGCTTGAGGAAGCTGTGAAGTCCATTCAGATGGAAGGTTTGTTTTGGGGAGCATCAAAGCTTGTCCCAGTTGGTTATGGTATCAAGAAGTTGCAGATTTTGTGCACCATTGTTGACGACCTTGTCTCTATTGACACCATGATCGAAGAGCAACTCACTGTTGAACCGATCAATGAATATGTCCAGAGCTGTGACATTGTTGCCTTCAACAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

231

Amino Acids

25.13

Weight (kDa)

4.43

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 144 - 231 4e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AciI CCGC 2 cut(s) 222, 302
AclWI GGATC 2 cut(s) 203, 247
AcuI CTGAAG 2 cut(s) 234, 393
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 4 cut(s) 40, 96, 641, 685
AjnI CCWGG 1 cut(s) 87
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 4 cut(s) 211, 341, 458, 607
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 2 cut(s) 203, 247
AlwNI CAGNNNCTG 1 cut(s) 389
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 6 cut(s) 5, 119, 281, 299, 368, 383
AsuHPI GGTGA 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 6 cut(s) 17, 131, 286, 293, 355, 395
BccI CCATC 1 cut(s) 502
BciT130I CCWGG 1 cut(s) 89
BciVI GTATCC 1 cut(s) 77
BcoDI GTCTC 4 cut(s) 211, 341, 458, 607
BfmI CTRYAG 1 cut(s) 279
BfuI GTATCC 1 cut(s) 77
BisI GCNGC 7 cut(s) 6, 120, 282, 300, 303, 369, 384
BlsI GCNGC 7 cut(s) 7, 121, 283, 301, 304, 370, 385
Bme1390I CCNGG 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 539
BmsI GCATC 6 cut(s) 19, 64, 172, 286, 407, 539
BmuI ACTGGG 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 211, 503
BsaBI GATNNNNATC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 452
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 2 cut(s) 85, 545
Bse8I GATNNNNATC 1 cut(s) 443
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 3 cut(s) 106, 301, 463
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 246, 444
BseNI ACTGG 2 cut(s) 85, 545
BseRI GAGGAG 2 cut(s) 272, 359
BseSI GKGCMC 1 cut(s) 584
BseXI GCAGC 6 cut(s) 17, 131, 286, 293, 355, 395
BsiHKAI GWGCWC 1 cut(s) 584
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 4 cut(s) 211, 341, 458, 607
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 4 cut(s) 195, 239, 618, 645
BspACI CCGC 2 cut(s) 222, 302
BspCNI CTCAG 2 cut(s) 247, 443
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 203, 247
BspQI GCTCTTC 1 cut(s) 618
BspTNI GGTCTC 1 cut(s) 341
BsrI ACTGG 2 cut(s) 85, 545
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 4 cut(s) 195, 239, 618, 645
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 4 cut(s) 112, 147, 453, 637
Bst6I CTCTTC 3 cut(s) 348, 369, 618
BstAPI GCANNNNNTGC 1 cut(s) 125
BstDEI CTNAG 3 cut(s) 255, 291, 430
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 3 cut(s) 106, 301, 463
BstKTI GATC 4 cut(s) 198, 242, 621, 648
BstMAI GTCTC 4 cut(s) 211, 341, 458, 607
BstMBI GATC 4 cut(s) 195, 239, 618, 645
BstMWI GCNNNNNNNGC 2 cut(s) 52, 125
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 584
BstV1I GCAGC 6 cut(s) 17, 131, 286, 293, 355, 395
BstX2I RGATCY 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 1 cut(s) 195
BsuI GTATCC 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 3 cut(s) 106, 301, 463
BtsIMutI CAGTG 2 cut(s) 252, 633
CaiI CAGNNNCTG 1 cut(s) 389
Csp6I GTAC 1 cut(s) 169
CviAII CATG 2 cut(s) 472, 616
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 3 cut(s) 255, 291, 430
DpnI GATC 4 cut(s) 197, 241, 620, 647
DpnII GATC 4 cut(s) 195, 239, 618, 645
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
Eam1104I CTCTTC 3 cut(s) 348, 369, 618
EarI CTCTTC 3 cut(s) 348, 369, 618
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 2 cut(s) 106, 445
Eco57I CTGAAG 2 cut(s) 234, 393
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 2 cut(s) 106, 445
FaeI CATG 2 cut(s) 475, 619
FaiI YATR 6 cut(s) 150, 473, 555, 617, 657, 694
FaqI GGGAC 1 cut(s) 527
FatI CATG 2 cut(s) 471, 615
Fnu4HI GCNGC 7 cut(s) 6, 120, 282, 300, 303, 369, 384
FokI GGATG 3 cut(s) 113, 308, 470
Fsp4HI GCNGC 7 cut(s) 6, 120, 282, 300, 303, 369, 384
GluI GCNGC 7 cut(s) 6, 120, 282, 300, 303, 369, 384
Hin1II CATG 2 cut(s) 475, 619
HincII GTYRAC 2 cut(s) 331, 592
HindII GTYRAC 2 cut(s) 331, 592
HindIII AAGCTT 2 cut(s) 479, 536
HinfI GANTC 2 cut(s) 287, 416
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 4 cut(s) 154, 331, 582, 592
Hpy188I TCNGA 3 cut(s) 28, 341, 507
Hpy188III TCNNGA 4 cut(s) 190, 420, 562, 661
Hpy8I GTNNAC 4 cut(s) 154, 331, 582, 592
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 209, 406, 506, 691
HpyCH4III ACNGT 4 cut(s) 112, 147, 453, 637
HpyCH4V TGCA 5 cut(s) 119, 128, 281, 571, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 125
HpyF3I CTNAG 3 cut(s) 255, 291, 430
Hsp92II CATG 2 cut(s) 475, 619
Kzo9I GATC 4 cut(s) 195, 239, 618, 645
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 2 cut(s) 250, 527
LpnPI CCDG 9 cut(s) 18, 66, 74, 101, 186, 291, 405, 558, 674
Lsp1109I GCAGC 6 cut(s) 17, 131, 286, 293, 355, 395
LweI GCATC 6 cut(s) 19, 64, 172, 286, 407, 539
MaeIII GTNAC 2 cut(s) 71, 668
MalI GATC 4 cut(s) 197, 241, 620, 647
MboI GATC 4 cut(s) 195, 239, 618, 645
MboII GAAGA 9 cut(s) 50, 52, 323, 326, 365, 386, 421, 487, 635
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 1 cut(s) 584
MnlI CCTC 9 cut(s) 186, 197, 229, 250, 253, 337, 349, 439, 478
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 2 cut(s) 180, 506
MspA1I CMGCKG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 52, 125
NdeII GATC 4 cut(s) 195, 239, 618, 645
NlaIII CATG 2 cut(s) 475, 619
NmuCI GTSAC 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 618
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 287, 416
PflFI GACNNNGTC 1 cut(s) 599
PkrI GCNGC 7 cut(s) 7, 121, 283, 301, 304, 370, 385
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 195
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 2 cut(s) 180, 506
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 7 cut(s) 6, 120, 282, 300, 303, 369, 384
Sau3AI GATC 4 cut(s) 195, 239, 618, 645
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 584
SfaNI GCATC 6 cut(s) 19, 64, 172, 286, 407, 539
SfcI CTRYAG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 180, 506
SmlI CTYRAG 2 cut(s) 190, 482
SmoI CTYRAG 2 cut(s) 190, 482
SsiI CCGC 2 cut(s) 222, 302
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 112, 147, 453, 637
TaqI TCGA 2 cut(s) 48, 621
TaqII GACCGA 1 cut(s) 365
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 2 cut(s) 287, 416
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 259, 640
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 6 cut(s) 5, 119, 281, 299, 368, 383
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 3 cut(s) 324, 488, 666
TspRI CASTG 2 cut(s) 259, 640
Tth111I GACNNNGTC 1 cut(s) 599
VneI GTGCAC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.