Rorug01G0002400

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003320
Physical Location & Seq
Forward (+)
546517 .. 549410
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0002400.1

Sequence Viewer

Length: 621 bp
ATGGAGATGGGTCATCAGCAGACGTTCTGGTGCCACGAGTGCGACATGAGCGTGAGTCTGCCGACGACGACGACGACGACGAGTGTTGTCTGTCCTCACTGCTTCAGCGACCTCCTGGAGCTGATGGATTCGAGTAGTGGCGACAATTTCCAAGCTCCAGAAGCATTCCGCCGCCCCCACGATCAGGACAACTACCGCCTCAACAGCCCAGTCCTCCAACGGCTGATCCACCGCCTCTCCGAGGACGACGTCGTCGTTCCGCCGCCCCCCGCCAATACCTACTTACTGCCGGCTTCCAAGGCGTACGTAGACGTCATCCCAACGGTGAGGATGACGTCACGTTCCATGTCGGTGTGCGCTGTTTGTAAGGAGCAATTTGCGGAGGACGCGGAGGCTAAGCAGCTGGCTTGCAACCACGTCTACCATCCTGACTGCATTCTCCCTTGGCTTTCTTCCCGGAGTTCGTGCCCCCTCTGCCGCTATCAGCTCCCCACCGACGACCACCACGCTCCTCCTCAACAAGACTCCCAACACCGAATTCAACGTCTGGTGCATTACCGACTTCAACTTCAACTCCAGCTCCAGCTCGACCGCTCATCCTTTAATGGTGGTTTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

206

Amino Acids

23.46

Weight (kDa)

5.82

Isoelectric Point (pI)

78.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 7 - 42 1.2e-10 zinc-ribbon
zf-rbx1 PF12678 115 - 160 1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 119 - 160 3.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 119 - 159 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 119 - 159 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 119 - 146 3e-06 RING-like zinc finger
zf-RING_UBOX PF13445 119 - 157 8.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 3 cut(s) 252, 315, 338
AccB1I GGYRCC 1 cut(s) 30
AccBSI CCGCTC 1 cut(s) 594
AccI GTMKAC 2 cut(s) 309, 420
AccII CGCG 1 cut(s) 389
AclWI GGATC 1 cut(s) 220
AcsI RAATTY 1 cut(s) 537
AcuI CTGAAG 1 cut(s) 88
AcyI GRCGYC 3 cut(s) 249, 312, 335
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 184, 241
AgsI TTSAA 3 cut(s) 542, 566, 572
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 1 cut(s) 114
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 6 cut(s) 121, 155, 403, 487, 580, 586
AluI AGCT 6 cut(s) 121, 155, 403, 487, 580, 586
AlwI GGATC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 537
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
AspLEI GCGC 1 cut(s) 359
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 30
BauI CACGAG 1 cut(s) 35
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 118, 432
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 116
BcnI CCSGG 1 cut(s) 457
BisI GCNGC 4 cut(s) 172, 263, 401, 478
BlpI GCTNAGC 1 cut(s) 396
BlsI GCNGC 4 cut(s) 173, 264, 402, 479
Bme1390I CCNGG 2 cut(s) 116, 457
BmgBI CACGTC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 2 cut(s) 116, 457
BmrI ACTGGG 1 cut(s) 203
BmuI ACTGGG 1 cut(s) 203
BpmI CTGGAG 4 cut(s) 137, 141, 560, 566
Bpu1102I GCTNAGC 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 307
BsaHI GRCGYC 3 cut(s) 249, 312, 335
BsaJI CCNNGG 3 cut(s) 240, 297, 443
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 558, 564, 588, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 241
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 240, 297, 443
BseGI GGATG 4 cut(s) 315, 336, 424, 596
BseLI CCNNNNNNNGG 2 cut(s) 184, 241
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 501, 504
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 1 cut(s) 412
Bsh1236I CGCG 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 592
BshNI GGYRCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 592
BsiSI CCGG 2 cut(s) 290, 457
BsiWI CGTACG 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 184, 241
BsmI GAATGC 2 cut(s) 164, 435
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 181, 225
Bsp1720I GCTNAGC 1 cut(s) 396
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 1 cut(s) 32
BspPI GGATC 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 240, 297, 443
BssMI GATC 2 cut(s) 181, 225
BssNI GRCGYC 3 cut(s) 249, 312, 335
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 2 cut(s) 297, 443
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 325, 618
BstACI GRCGYC 3 cut(s) 249, 312, 335
BstBAI YACGTR 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 291, 405, 409
BstDEI CTNAG 1 cut(s) 396
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 4 cut(s) 315, 336, 424, 596
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 2 cut(s) 184, 228
BstMBI GATC 2 cut(s) 181, 225
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 7 cut(s) 39, 48, 161, 204, 299, 386, 474
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 114, 455
BstSLI GKGCMC 1 cut(s) 470
BstSNI TACGTA 1 cut(s) 307
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 412
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 4 cut(s) 315, 336, 424, 596
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 291, 405, 409
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 289
CseI GACGC 1 cut(s) 395
Csp6I GTAC 1 cut(s) 304
CviAII CATG 2 cut(s) 46, 346
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 1 cut(s) 396
DpnI GATC 2 cut(s) 183, 227
DpnII GATC 2 cut(s) 181, 225
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EciI GGCGGA 2 cut(s) 158, 249
Eco105I TACGTA 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 297, 443
Eco57I CTGAAG 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 2 cut(s) 297, 443
ErhI CCWWGG 2 cut(s) 297, 443
FaeI CATG 2 cut(s) 49, 349
FaiI YATR 2 cut(s) 47, 347
FatI CATG 2 cut(s) 45, 345
FauI CCCGC 1 cut(s) 277
FblI GTMKAC 2 cut(s) 309, 420
Fnu4HI GCNGC 4 cut(s) 172, 263, 401, 478
FokI GGATG 4 cut(s) 302, 343, 411, 583
Fsp4HI GCNGC 4 cut(s) 172, 263, 401, 478
GlaI GCGC 1 cut(s) 358
GluI GCNGC 4 cut(s) 172, 263, 401, 478
GsuI CTGGAG 4 cut(s) 137, 141, 560, 566
HapII CCGG 2 cut(s) 290, 457
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 359
Hin1I GRCGYC 3 cut(s) 249, 312, 335
Hin1II CATG 2 cut(s) 49, 349
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HinfI GANTC 3 cut(s) 55, 128, 524
HpaII CCGG 2 cut(s) 290, 457
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 3 cut(s) 310, 421, 614
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 158, 185, 428
Hpy8I GTNNAC 3 cut(s) 310, 421, 614
HpyCH4III ACNGT 2 cut(s) 325, 618
HpyCH4IV ACGT 8 cut(s) 23, 249, 306, 312, 335, 340, 417, 544
HpyCH4V TGCA 3 cut(s) 411, 435, 553
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 48, 161, 204, 299, 386, 474
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 8 cut(s) 23, 249, 306, 312, 335, 340, 417, 544
Hsp92I GRCGYC 3 cut(s) 249, 312, 335
Hsp92II CATG 2 cut(s) 49, 349
HspAI GCGC 1 cut(s) 357
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 181, 225
LmnI GCTCC 6 cut(s) 118, 160, 370, 492, 514, 585
Lsp1109I GCAGC 1 cut(s) 412
MaeII ACGT 8 cut(s) 23, 249, 306, 312, 335, 340, 417, 544
MaeIII GTNAC 1 cut(s) 336
MalI GATC 2 cut(s) 183, 227
MbiI CCGCTC 1 cut(s) 594
MboI GATC 2 cut(s) 181, 225
MboII GAAGA 1 cut(s) 444
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 3 cut(s) 145, 374, 537
MlyI GAGTC 2 cut(s) 64, 518
MmeI TCCRAC 1 cut(s) 241
MroNI GCCGGC 1 cut(s) 289
MseI TTAA 1 cut(s) 603
MslI CAYNNNNRTG 2 cut(s) 50, 350
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 2 cut(s) 290, 457
MspR9I CCNGG 2 cut(s) 116, 457
Mva1269I GAATGC 2 cut(s) 164, 435
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 7 cut(s) 39, 48, 161, 204, 299, 386, 474
NaeI GCCGGC 1 cut(s) 291
NciI CCSGG 1 cut(s) 457
NdeII GATC 2 cut(s) 181, 225
NgoMIV GCCGGC 1 cut(s) 289
NlaIII CATG 2 cut(s) 49, 349
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 336
PcsI WCGNNNNNNNCGW 4 cut(s) 71, 74, 77, 252
PctI GAATGC 2 cut(s) 164, 435
PdiI GCCGGC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 128
Pfl23II CGTACG 1 cut(s) 303
PflFI GACNNNGTC 2 cut(s) 248, 251
PfoI TCCNGGA 2 cut(s) 114, 455
PkrI GCNGC 4 cut(s) 173, 264, 402, 479
PleI GAGTC 2 cut(s) 63, 518
PpsI GAGTC 2 cut(s) 63, 518
Ppu21I YACGTR 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspLI CGTACG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 32
PsyI GACNNNGTC 2 cut(s) 248, 251
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 50, 350
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 4 cut(s) 172, 263, 401, 478
Sau3AI GATC 2 cut(s) 181, 225
SchI GAGTC 2 cut(s) 64, 518
ScrFI CCNGG 2 cut(s) 116, 457
SduI GDGCHC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 50, 350
SnaBI TACGTA 1 cut(s) 307
Sse9I AATT 3 cut(s) 145, 374, 537
StyD4I CCNGG 2 cut(s) 114, 455
StyI CCWWGG 2 cut(s) 297, 443
TaaI ACNGT 2 cut(s) 325, 618
TaiI ACGT 8 cut(s) 26, 252, 309, 315, 338, 343, 420, 547
TaqI TCGA 2 cut(s) 131, 588
TasI AATT 3 cut(s) 145, 374, 537
TauI GCSGC 3 cut(s) 174, 265, 480
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TscAI CASTG 1 cut(s) 104
TseFI GTSAC 1 cut(s) 336
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 336
TspRI CASTG 1 cut(s) 104
Tth111I GACNNNGTC 2 cut(s) 248, 251
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 1 cut(s) 537
XmiI GTMKAC 2 cut(s) 309, 420
ZraI GACGTC 3 cut(s) 250, 313, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.