Rh1BG004800

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003320
Physical Location & Seq
Forward (+)
647561 .. 650072
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG004800.1

Sequence Viewer

Length: 594 bp
ATGGCAGTTGCATTCCATGACATCAACTCGGCTGCTGGCCTCAAGAAATTGGACCAGTACTTGCTCACTCGCAGTTACATCACTGGCTATCAGGCTTCAAAGGATGATGTCACTGTGCATGCTGCTCTGTCCAAGCCTCCATCGGCTGAATACGTGAATGCGTCTCGGTGGTACAACCACATTGCTGCACTTCTTAGGATTTCAGGTGTTTCTGGAGAAGGTTGTGGTGTCACAATTAAGGGATCTGCTCCCATCACAGCAGAAGCAGTTGCCACACCTCCAGCTTGTGATTCAAAGGCCTCTGCTGCTGAAGATGATGATGATGATAGTGATGTGGATCTCTTTGGTGAAGAGACCGAAGAGGAGAAGAAGGCTGCTGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAAGAGTCTGGCAAGTCATCAGTTCTATTGGATGTTAAGCCATGGGATGATGAAACAGACATGAAAAAGCTTGAGGAGGCAGTAAGGAGCATTGAGGTTGAGGGCTTATTTTGGGGAGCATGTAAGTTTCCTACCCCCACCCCATATATGGATAATTTAGCTAGAGAAGCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

197

Amino Acids

21.18

Weight (kDa)

4.73

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 146 - 181 1.5e-10 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 250, 345
AcuI CTGAAG 2 cut(s) 330, 399
AfaI GTAC 2 cut(s) 59, 173
AfiI CCNNNNNNNGG 1 cut(s) 565
AgsI TTSAA 2 cut(s) 99, 294
AluBI AGCT 5 cut(s) 284, 392, 487, 578, 587
AluI AGCT 5 cut(s) 284, 392, 487, 578, 587
Alw26I GTCTC 2 cut(s) 168, 347
AlwI GGATC 2 cut(s) 250, 345
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 2 cut(s) 37, 297
ApeKI GCWGC 7 cut(s) 32, 122, 185, 305, 374, 389, 392
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BccI CCATC 2 cut(s) 148, 260
BcoDI GTCTC 2 cut(s) 168, 347
BfaI CTAG 1 cut(s) 579
BisI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
BlsI GCNGC 7 cut(s) 34, 124, 187, 307, 376, 391, 394
BmcAI AGTACT 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmsI GCATC 1 cut(s) 413
BpmI CTGGAG 2 cut(s) 234, 264
BpuEI CTTGAG 2 cut(s) 26, 509
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 458
Bsc4I CCNNNNNNNGG 1 cut(s) 565
Bse1I ACTGG 2 cut(s) 55, 88
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 458
BseGI GGATG 3 cut(s) 109, 454, 469
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 565
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 55, 88
BseRI GAGGAG 2 cut(s) 377, 506
BseXI GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BsgI GTGCAG 2 cut(s) 171, 408
BshFI GGCC 2 cut(s) 39, 299
BslI CCNNNNNNNGG 1 cut(s) 565
BsmAI GTCTC 2 cut(s) 168, 347
BsmBI CGTCTC 1 cut(s) 168
BsmI GAATGC 2 cut(s) 11, 163
BsnI GGCC 2 cut(s) 39, 299
Bso31I GGTCTC 1 cut(s) 347
Bsp143I GATC 2 cut(s) 242, 337
Bsp19I CCATGG 1 cut(s) 458
BspANI GGCC 2 cut(s) 39, 299
BspPI GGATC 2 cut(s) 250, 345
BspTNI GGTCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 2 cut(s) 55, 88
BssECI CCNNGG 1 cut(s) 458
BssMI GATC 2 cut(s) 242, 337
BssT1I CCWWGG 1 cut(s) 458
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 2 cut(s) 345, 354
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 2 cut(s) 194, 591
BstDSI CCRYGG 1 cut(s) 458
BstF5I GGATG 3 cut(s) 109, 454, 469
BstKTI GATC 2 cut(s) 245, 340
BstMAI GTCTC 2 cut(s) 168, 347
BstMBI GATC 2 cut(s) 242, 337
BstMWI GCNNNNNNNGC 3 cut(s) 305, 401, 584
BstNSI RCATGY 2 cut(s) 122, 540
BstV1I GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BstX2I RGATCY 2 cut(s) 242, 337
BstYI RGATCY 2 cut(s) 242, 337
BsuRI GGCC 2 cut(s) 39, 299
BtgI CCRYGG 1 cut(s) 458
BtsCI GGATG 3 cut(s) 109, 454, 469
BtsIMutI CAGTG 2 cut(s) 81, 111
Cac8I GCNNGC 2 cut(s) 37, 120
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 150
Csp6I GTAC 2 cut(s) 58, 172
CviAII CATG 5 cut(s) 17, 119, 459, 478, 537
CviQI GTAC 2 cut(s) 58, 172
DdeI CTNAG 2 cut(s) 194, 591
DpnI GATC 2 cut(s) 244, 339
DpnII GATC 2 cut(s) 242, 337
Eam1104I CTCTTC 2 cut(s) 345, 354
EarI CTCTTC 2 cut(s) 345, 354
Eco130I CCWWGG 1 cut(s) 458
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 330, 399
EcoT14I CCWWGG 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 458
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 20, 122, 462, 481, 540
FaiI YATR 8 cut(s) 18, 120, 460, 479, 538, 562, 564, 566
FatI CATG 5 cut(s) 16, 118, 458, 477, 536
Fnu4HI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
FokI GGATG 3 cut(s) 116, 461, 476
Fsp4HI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
FspBI CTAG 1 cut(s) 579
GluI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
GsuI CTGGAG 2 cut(s) 234, 264
HaeIII GGCC 2 cut(s) 39, 299
HgaI GACGC 1 cut(s) 150
Hin1II CATG 5 cut(s) 20, 122, 462, 481, 540
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 2 cut(s) 290, 422
HphI GGTGA 1 cut(s) 359
Hpy188III TCNNGA 2 cut(s) 43, 213
HpyAV CCTTC 2 cut(s) 212, 364
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 153, 385
HpyCH4V TGCA 4 cut(s) 11, 118, 188, 389
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 401, 584
HpyF3I CTNAG 2 cut(s) 194, 591
HpySE526I ACGT 2 cut(s) 153, 385
Hsp92II CATG 5 cut(s) 20, 122, 462, 481, 540
Kzo9I GATC 2 cut(s) 242, 337
LmnI GCTCC 3 cut(s) 253, 504, 533
LpnPI CCDG 8 cut(s) 21, 68, 69, 77, 189, 198, 294, 411
Lsp1109I GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
LweI GCATC 1 cut(s) 413
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 153, 385
MaeIII GTNAC 3 cut(s) 74, 109, 229
MalI GATC 2 cut(s) 244, 339
MboI GATC 2 cut(s) 242, 337
MboII GAAGA 5 cut(s) 323, 362, 371, 379, 392
MflI RGATCY 2 cut(s) 242, 337
MluCI AATT 3 cut(s) 47, 234, 571
MlyI GAGTC 1 cut(s) 431
MnlI CCTC 9 cut(s) 50, 147, 288, 310, 355, 484, 487, 505, 511
MseI TTAA 2 cut(s) 237, 453
MspA1I CMGCKG 1 cut(s) 392
Mva1269I GAATGC 2 cut(s) 11, 163
MwoI GCNNNNNNNGC 3 cut(s) 305, 401, 584
NcoI CCATGG 1 cut(s) 458
NdeII GATC 2 cut(s) 242, 337
NlaIII CATG 5 cut(s) 20, 122, 462, 481, 540
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 109, 229
NspI RCATGY 2 cut(s) 122, 540
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 2 cut(s) 11, 163
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 7 cut(s) 34, 124, 187, 307, 376, 391, 394
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 154
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 2 cut(s) 242, 337
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 59, 173
RsaNI GTAC 2 cut(s) 58, 172
SaqAI TTAA 2 cut(s) 237, 453
SatI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
Sau3AI GATC 2 cut(s) 242, 337
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 1 cut(s) 431
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 2 cut(s) 41, 488
SmoI CTYRAG 2 cut(s) 41, 488
SphI GCATGC 1 cut(s) 122
Sse9I AATT 3 cut(s) 47, 234, 571
SseBI AGGCCT 1 cut(s) 299
SspMI CTAG 1 cut(s) 579
StuI AGGCCT 1 cut(s) 299
StyI CCWWGG 1 cut(s) 458
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 156, 388
TaqII GACCGA 1 cut(s) 371
TasI AATT 3 cut(s) 47, 234, 571
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 237, 453
Tru9I TTAA 2 cut(s) 237, 453
TscAI CASTG 2 cut(s) 88, 118
TseFI GTSAC 2 cut(s) 109, 229
TseI GCWGC 7 cut(s) 32, 122, 185, 305, 374, 389, 392
Tsp45I GTSAC 2 cut(s) 109, 229
TspDTI ATGAA 2 cut(s) 483, 494
TspRI CASTG 2 cut(s) 88, 118
VpaK11BI GGWCC 1 cut(s) 52
XceI RCATGY 2 cut(s) 122, 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XspI CTAG 1 cut(s) 579
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.