Rh1AG013200

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003320
Physical Location & Seq
Forward (+)
2130135 .. 2132255
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG013200.1

Sequence Viewer

Length: 702 bp
ATGGCAGTTGCATTCCATGACATCAACTCGGCTGCTGGCCTCAAGAAATTGGACCAGTACTTGCTCACTCGCAGTTACATCACTGGCTATCAGGCTTCTAAGGATGATGTCACTGTGCATGCTGCTCTGTCCAAACCTCCATCAGCTGAATACGTGAATGCGTCTCGGTGGTACAACCACATTGCTGCACTTCTTAGGATTTCGGGTGTTTCTGGAGAAGGTTGTGGTGTCAAAATTGAGGGATCTGCTCCCATCACAGCAGAAGCAGTTGCCACCCCTCCAGCTTGTGATTCAAAGGCCTCTGCTGCTGAAGATGATGATGATGATAGTGATGTGGATCTGTTTGGTGAAGAGACTGAAGAGGAGAAGAAGGCTGCCGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAAGAGTCTGGCAAGTCATCAGTTCTATTGGATATTAAGCCATGGGACGATGAAACAGACATGAAAAAGCTTGAGGAGGCAGTAAGGAGCATTGAGGTTGAGGGCTTACATTGGGGAGCATCTAAACTAGTACCTGTTGGGTATGGCATAAAGAAGTTGACAATTATGCTTACAATTGTTGATGACCTCGTTTCTGTGGACTCTCTGATTGAAGAACGTCTTACCGTGGAGCCTATTAATGAATATGTCCAGAGCTGTGACATTGTTGCCTTCAATAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

233

Amino Acids

25.18

Weight (kDa)

4.67

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 146 - 233 1.8e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 250, 345
AcuI CTGAAG 2 cut(s) 330, 378
AfaI GTAC 3 cut(s) 59, 173, 549
AgsI TTSAA 3 cut(s) 294, 629, 691
AhlI ACTAGT 1 cut(s) 544
AluBI AGCT 5 cut(s) 146, 284, 392, 487, 672
AluI AGCT 5 cut(s) 146, 284, 392, 487, 672
Alw26I GTCTC 2 cut(s) 168, 347
AlwI GGATC 2 cut(s) 250, 345
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 2 cut(s) 37, 297
ApeKI GCWGC 7 cut(s) 32, 122, 185, 305, 374, 389, 392
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BccI CCATC 2 cut(s) 148, 260
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BcoDI GTCTC 2 cut(s) 168, 347
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 1 cut(s) 545
BisI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
BlsI GCNGC 7 cut(s) 34, 124, 187, 307, 376, 391, 394
BmcAI AGTACT 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 648
BmsI GCATC 2 cut(s) 413, 545
BpmI CTGGAG 2 cut(s) 234, 264
BpuEI CTTGAG 2 cut(s) 26, 509
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 458, 642
Bse1I ACTGG 2 cut(s) 55, 88
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 458, 642
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 55, 88
BseRI GAGGAG 2 cut(s) 377, 506
BseXI GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BsgI GTGCAG 2 cut(s) 171, 408
BshFI GGCC 2 cut(s) 39, 299
BslFI GGGAC 1 cut(s) 476
BsmAI GTCTC 2 cut(s) 168, 347
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 476
BsmI GAATGC 2 cut(s) 11, 163
BsnI GGCC 2 cut(s) 39, 299
Bsp143I GATC 2 cut(s) 242, 337
Bsp19I CCATGG 1 cut(s) 458
BspANI GGCC 2 cut(s) 39, 299
BspLI GGNNCC 1 cut(s) 648
BspPI GGATC 2 cut(s) 250, 345
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 2 cut(s) 55, 88
BssECI CCNNGG 2 cut(s) 458, 642
BssMI GATC 2 cut(s) 242, 337
BssT1I CCWWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 115, 643
Bst6I CTCTTC 2 cut(s) 345, 354
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 2 cut(s) 99, 194
BstDSI CCRYGG 2 cut(s) 458, 642
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 2 cut(s) 245, 340
BstMAI GTCTC 2 cut(s) 168, 347
BstMBI GATC 2 cut(s) 242, 337
BstMWI GCNNNNNNNGC 2 cut(s) 305, 401
BstNSI RCATGY 1 cut(s) 122
BstV1I GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
BstX2I RGATCY 2 cut(s) 242, 337
BstYI RGATCY 2 cut(s) 242, 337
BsuRI GGCC 2 cut(s) 39, 299
BtgI CCRYGG 2 cut(s) 458, 642
BtsCI GGATG 1 cut(s) 109
BtsIMutI CAGTG 2 cut(s) 81, 111
Cac8I GCNNGC 2 cut(s) 37, 120
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 150
Csp6I GTAC 3 cut(s) 58, 172, 548
CviAII CATG 4 cut(s) 17, 119, 459, 478
CviQI GTAC 3 cut(s) 58, 172, 548
DdeI CTNAG 2 cut(s) 99, 194
DpnI GATC 2 cut(s) 244, 339
DpnII GATC 2 cut(s) 242, 337
Eam1104I CTCTTC 2 cut(s) 345, 354
EarI CTCTTC 2 cut(s) 345, 354
Eco130I CCWWGG 1 cut(s) 458
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 330, 378
EcoT14I CCWWGG 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 458
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 4 cut(s) 20, 122, 462, 481
FaiI YATR 9 cut(s) 18, 120, 460, 479, 561, 566, 584, 663, 700
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 1 cut(s) 476
FatI CATG 4 cut(s) 16, 118, 458, 477
Fnu4HI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
FspBI CTAG 1 cut(s) 545
GluI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
GsuI CTGGAG 2 cut(s) 234, 264
HaeIII GGCC 2 cut(s) 39, 299
HgaI GACGC 1 cut(s) 150
Hin1II CATG 4 cut(s) 20, 122, 462, 481
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 290, 422, 617
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 576, 616
Hpy188I TCNGA 1 cut(s) 624
Hpy188III TCNNGA 3 cut(s) 43, 213, 667
Hpy8I GTNNAC 2 cut(s) 576, 616
HpyAV CCTTC 3 cut(s) 212, 364, 697
HpyCH4III ACNGT 2 cut(s) 115, 643
HpyCH4IV ACGT 3 cut(s) 153, 385, 634
HpyCH4V TGCA 4 cut(s) 11, 118, 188, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 401
HpyF3I CTNAG 2 cut(s) 99, 194
HpySE526I ACGT 3 cut(s) 153, 385, 634
Hsp92II CATG 4 cut(s) 20, 122, 462, 481
Kzo9I GATC 2 cut(s) 242, 337
LmnI GCTCC 4 cut(s) 253, 504, 533, 646
LpnPI CCDG 9 cut(s) 21, 68, 69, 77, 198, 294, 411, 564, 680
Lsp1109I GCAGC 7 cut(s) 19, 109, 172, 292, 361, 379, 401
LweI GCATC 2 cut(s) 413, 545
MaeI CTAG 1 cut(s) 545
MaeII ACGT 3 cut(s) 153, 385, 634
MaeIII GTNAC 3 cut(s) 74, 109, 674
MalI GATC 2 cut(s) 244, 339
MboI GATC 2 cut(s) 242, 337
MboII GAAGA 6 cut(s) 323, 362, 371, 379, 392, 641
MfeI CAATTG 1 cut(s) 591
MflI RGATCY 2 cut(s) 242, 337
MluCI AATT 4 cut(s) 47, 234, 579, 591
MlyI GAGTC 2 cut(s) 431, 611
MseI TTAA 2 cut(s) 453, 654
MspA1I CMGCKG 2 cut(s) 146, 392
MunI CAATTG 1 cut(s) 591
Mva1269I GAATGC 2 cut(s) 11, 163
MwoI GCNNNNNNNGC 2 cut(s) 305, 401
NcoI CCATGG 1 cut(s) 458
NdeII GATC 2 cut(s) 242, 337
NlaIII CATG 4 cut(s) 20, 122, 462, 481
NlaIV GGNNCC 1 cut(s) 648
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 109, 674
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 2 cut(s) 11, 163
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 7 cut(s) 34, 124, 187, 307, 376, 391, 394
PleI GAGTC 2 cut(s) 430, 611
PpsI GAGTC 2 cut(s) 430, 611
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 654
PspN4I GGNNCC 1 cut(s) 648
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 2 cut(s) 242, 337
PvuII CAGCTG 2 cut(s) 146, 392
RsaI GTAC 3 cut(s) 59, 173, 549
RsaNI GTAC 3 cut(s) 58, 172, 548
SaqAI TTAA 2 cut(s) 453, 654
SatI GCNGC 7 cut(s) 33, 123, 186, 306, 375, 390, 393
Sau3AI GATC 2 cut(s) 242, 337
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 2 cut(s) 431, 611
SfaNI GCATC 2 cut(s) 413, 545
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 2 cut(s) 41, 488
SmoI CTYRAG 2 cut(s) 41, 488
SpeI ACTAGT 1 cut(s) 544
SphI GCATGC 1 cut(s) 122
Sse9I AATT 4 cut(s) 47, 234, 579, 591
SseBI AGGCCT 1 cut(s) 299
SspMI CTAG 1 cut(s) 545
StuI AGGCCT 1 cut(s) 299
StyI CCWWGG 1 cut(s) 458
TaaI ACNGT 2 cut(s) 115, 643
TaiI ACGT 3 cut(s) 156, 388, 637
TasI AATT 4 cut(s) 47, 234, 579, 591
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 453, 654
Tru9I TTAA 2 cut(s) 453, 654
TscAI CASTG 2 cut(s) 88, 118
TseFI GTSAC 2 cut(s) 109, 674
TseI GCWGC 7 cut(s) 32, 122, 185, 305, 374, 389, 392
Tsp45I GTSAC 2 cut(s) 109, 674
TspDTI ATGAA 3 cut(s) 483, 494, 672
TspRI CASTG 2 cut(s) 88, 118
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 654
XceI RCATGY 1 cut(s) 122
XspI CTAG 1 cut(s) 545
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.