RLG00000004537

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003320
Physical Location & Seq
Forward (+)
60112826 .. 60114815
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004537

Sequence Viewer

Length: 705 bp
ATGCCCACAGCCCCTGTCACTGTCACTATCACTATCACTGTCACTCCCTATAAACCCAGGAAAAGAGTGCCGCGCGCACTGCTTATCTCCAAACTGTCACTATCCATCATCTTCTCTCGTCGGTATCAGGCTTCAAAGGATGATATCACTGTGCTTGCAGCTCTTGCAAAACCCCCATCCTCGGATTCTCGGAATATTGGTGTTTCCGGAGAAGGTTGTGGTGTCACCATCAAGGGAGATGCTCCTGTCATAGCAGCTGAAACGAAGGCAAGTCCAGCCTCAGCTGCTGAAGAAGATGATGATGATGTGGATCTTTTTGGTGAAGAAACCGAAGAGGAGAAGAAGGCTGCTCAAGAACGTGCAGCCTCCATCAACGCATCTGCAAAAAAGAAAGAGTCCGGCAAGTCGTCAGTTCTGATGGATGTCAAACCATGGGATGATGAAACCGACATGCAAAAGCTTTTGGAAGCAGTAAAAAGTGTTCAGATGGAAGGTTTGCATTGGGGAGCATCCAAACTTGTACCTGTTGGGTACGGGATAAAGAAGCTGCAGATAATGCTAACAATTGTTGATGACCTGGTCTCTGTGGACACTCTCAGTGAGGAACATCTCACAGTTGAACCGATTAATGAGTATGTCCAAAGCTGCGATATTGTAGCCTTCAACAAAATATGTACGTATAGTTTCCTTGTACTTTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

235

Amino Acids

25.37

Weight (kDa)

4.96

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 137 - 224 3.6e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 73, 75
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 1 cut(s) 71
AclWI GGATC 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 4 cut(s) 522, 533, 676, 693
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 3 cut(s) 135, 620, 664
AjnI CCWGG 2 cut(s) 56, 576
AluBI AGCT 6 cut(s) 161, 257, 284, 460, 547, 645
AluI AGCT 6 cut(s) 161, 257, 284, 460, 547, 645
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 1 cut(s) 318
AlwNI CAGNNNCTG 2 cut(s) 14, 287
Aor13HI TCCGGA 1 cut(s) 206
ApeKI GCWGC 7 cut(s) 158, 254, 284, 347, 362, 547, 645
AseI ATTAAT 1 cut(s) 627
AspLEI GCGC 2 cut(s) 75, 77
AsuHPI GGTGA 2 cut(s) 217, 332
BbvCI CCTCAGC 1 cut(s) 280
BbvI GCAGC 7 cut(s) 170, 266, 271, 334, 374, 534, 632
BccI CCATC 6 cut(s) 113, 184, 236, 377, 412, 481
BciT130I CCWGG 2 cut(s) 58, 578
BcoDI GTCTC 1 cut(s) 586
BfmI CTRYAG 1 cut(s) 548
BisI GCNGC 8 cut(s) 71, 159, 255, 285, 348, 363, 548, 646
BlsI GCNGC 8 cut(s) 72, 160, 256, 286, 349, 364, 549, 647
Bme1390I CCNGG 2 cut(s) 58, 578
BmrFI CCNGG 2 cut(s) 58, 578
BmsI GCATC 3 cut(s) 229, 386, 518
Bpu10I CCTNAGC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 586
BsaJI CCNNGG 3 cut(s) 56, 180, 431
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 206
BseBI CCWGG 2 cut(s) 58, 578
BseDI CCNNGG 3 cut(s) 56, 180, 431
BseGI GGATG 5 cut(s) 145, 176, 427, 442, 509
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 294, 610
BsePI GCGCGC 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 7 cut(s) 170, 266, 271, 334, 374, 534, 632
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 2 cut(s) 73, 75
BsiSI CCGG 2 cut(s) 207, 399
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 586
Bso31I GGTCTC 1 cut(s) 586
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 1 cut(s) 71
BspCNI CTCAG 2 cut(s) 293, 609
BspEI TCCGGA 1 cut(s) 206
BspFNI CGCG 2 cut(s) 73, 75
BspMAI CTGCAG 1 cut(s) 552
BspPI GGATC 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 586
BssECI CCNNGG 3 cut(s) 56, 180, 431
BssHII GCGCGC 1 cut(s) 73
BssMI GATC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 58, 578
Bst4CI ACNGT 5 cut(s) 22, 40, 96, 151, 616
Bst6I CTCTTC 1 cut(s) 327
BstAPI GCANNNNNTGC 2 cut(s) 164, 556
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 75, 156
BstDEI CTNAG 2 cut(s) 280, 596
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 5 cut(s) 145, 176, 427, 442, 509
BstFNI CGCG 2 cut(s) 73, 75
BstHHI GCGC 2 cut(s) 75, 77
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 5 cut(s) 79, 164, 275, 284, 556
BstNI CCWGG 2 cut(s) 58, 578
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 2 cut(s) 56, 576
BstSFI CTRYAG 1 cut(s) 548
BstSNI TACGTA 1 cut(s) 678
BstUI CGCG 2 cut(s) 73, 75
BstV1I GCAGC 7 cut(s) 170, 266, 271, 334, 374, 534, 632
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 431
BtsCI GGATG 5 cut(s) 145, 176, 427, 442, 509
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 5 cut(s) 18, 36, 77, 147, 604
Cac8I GCNNGC 2 cut(s) 75, 156
CaiI CAGNNNCTG 2 cut(s) 14, 287
CfoI GCGC 2 cut(s) 75, 77
CsiI ACCWGGT 1 cut(s) 576
Csp6I GTAC 4 cut(s) 521, 532, 675, 692
CviAII CATG 2 cut(s) 432, 451
CviQI GTAC 4 cut(s) 521, 532, 675, 692
DdeI CTNAG 2 cut(s) 280, 596
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 678
Eco130I CCWWGG 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 586
Eco32I GATATC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 56, 576
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 2 cut(s) 435, 454
FaiI YATR 7 cut(s) 51, 251, 433, 452, 636, 673, 681
FatI CATG 2 cut(s) 431, 450
Fnu4HI GCNGC 8 cut(s) 71, 159, 255, 285, 348, 363, 548, 646
FokI GGATG 5 cut(s) 152, 163, 434, 449, 496
Fsp4HI GCNGC 8 cut(s) 71, 159, 255, 285, 348, 363, 548, 646
GlaI GCGC 2 cut(s) 74, 76
GluI GCNGC 8 cut(s) 71, 159, 255, 285, 348, 363, 548, 646
HapII CCGG 2 cut(s) 207, 399
HhaI GCGC 2 cut(s) 75, 77
Hin1II CATG 2 cut(s) 435, 454
Hin6I GCGC 2 cut(s) 73, 75
HinP1I GCGC 2 cut(s) 73, 75
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 2 cut(s) 185, 395
HpaII CCGG 2 cut(s) 207, 399
HphI GGTGA 2 cut(s) 217, 332
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 4 cut(s) 184, 192, 417, 486
Hpy188III TCNNGA 2 cut(s) 207, 353
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 5 cut(s) 206, 259, 337, 485, 670
HpyCH4III ACNGT 5 cut(s) 22, 40, 96, 151, 616
HpyCH4IV ACGT 2 cut(s) 358, 677
HpyCH4V TGCA 8 cut(s) 158, 167, 362, 383, 454, 499, 550, 700
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 164, 275, 284, 556
HpyF3I CTNAG 2 cut(s) 280, 596
HpySE526I ACGT 2 cut(s) 358, 677
Hsp92II CATG 2 cut(s) 435, 454
HspAI GCGC 2 cut(s) 73, 75
Kpn2I TCCGGA 1 cut(s) 206
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 247, 506
Lsp1109I GCAGC 7 cut(s) 170, 266, 271, 334, 374, 534, 632
LweI GCATC 3 cut(s) 229, 386, 518
MabI ACCWGGT 1 cut(s) 576
MaeII ACGT 2 cut(s) 358, 677
MaeIII GTNAC 5 cut(s) 16, 22, 40, 96, 223
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 6 cut(s) 103, 302, 305, 335, 344, 352
MfeI CAATTG 1 cut(s) 564
MflI RGATCY 1 cut(s) 310
MluCI AATT 1 cut(s) 564
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 5 cut(s) 190, 289, 328, 376, 595
MroI TCCGGA 1 cut(s) 206
MseI TTAA 1 cut(s) 627
MspA1I CMGCKG 2 cut(s) 257, 284
MspI CCGG 2 cut(s) 207, 399
MspR9I CCNGG 2 cut(s) 58, 578
MunI CAATTG 1 cut(s) 564
MvaI CCWGG 2 cut(s) 58, 578
MvnI CGCG 2 cut(s) 73, 75
MwoI GCNNNNNNNGC 5 cut(s) 79, 164, 275, 284, 556
NcoI CCATGG 1 cut(s) 431
NdeII GATC 1 cut(s) 310
NlaIII CATG 2 cut(s) 435, 454
NmuCI GTSAC 5 cut(s) 16, 22, 40, 96, 223
NspI RCATGY 1 cut(s) 454
PauI GCGCGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 8 cut(s) 72, 160, 256, 286, 349, 364, 549, 647
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 678
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 56, 576
PspGI CCWGG 2 cut(s) 56, 576
PsrI GAACNNNNNNTAC 2 cut(s) 465, 497
PstI CTGCAG 1 cut(s) 552
PstNI CAGNNNCTG 2 cut(s) 14, 287
PsuI RGATCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 578
PteI GCGCGC 1 cut(s) 73
PvuII CAGCTG 2 cut(s) 257, 284
RsaI GTAC 4 cut(s) 522, 533, 676, 693
RsaNI GTAC 4 cut(s) 521, 532, 675, 692
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 8 cut(s) 71, 159, 255, 285, 348, 363, 548, 646
Sau3AI GATC 1 cut(s) 310
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 2 cut(s) 58, 578
SexAI ACCWGGT 1 cut(s) 576
SfaNI GCATC 3 cut(s) 229, 386, 518
SfcI CTRYAG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
SnaBI TACGTA 1 cut(s) 678
Sse9I AATT 1 cut(s) 564
SsiI CCGC 1 cut(s) 71
SspI AATATT 1 cut(s) 196
StyD4I CCNGG 2 cut(s) 56, 576
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 5 cut(s) 22, 40, 96, 151, 616
TaiI ACGT 2 cut(s) 361, 680
TasI AATT 1 cut(s) 564
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TscAI CASTG 5 cut(s) 25, 43, 84, 154, 604
TseFI GTSAC 5 cut(s) 16, 22, 40, 96, 223
TseI GCWGC 7 cut(s) 158, 254, 284, 347, 362, 547, 645
Tsp45I GTSAC 5 cut(s) 16, 22, 40, 96, 223
TspDTI ATGAA 1 cut(s) 456
TspRI CASTG 5 cut(s) 25, 43, 84, 154, 604
Tth111I GACNNNGTC 1 cut(s) 578
VspI ATTAAT 1 cut(s) 627
XceI RCATGY 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.