Rw1G000590

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003320
Physical Location & Seq
Forward (+)
904774 .. 906146
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000590.1

Sequence Viewer

Length: 780 bp
ATGGAAGTTGCATTCCATGACATCAACTCAGCTGCTGGCCTCAAGAATTTGGACCAGTACTTGCTCACTCGCAGTTACATCACCGGCTATCAGGCTTCGAAGGATGATATCACTGTGCATGCTGCTCTGTCCAAGACTCCATCAGCTGAATACGTGAATGCATCTCGGTGGTACAACCACATTGCTGCACTTCTTAGGATTTCTTTGCGTTTGAATGATATCTGGTGTTGTGGGGGTGTTTCTGGAGAAGGTTGTGGTTTCACAATTGAGGGATCTGCTCCCATCACAGGAGAAGCCGCTGCCACACCTCCAACTGGTGGTTCAAAGGCCTCTGCTGCTGAAGATGATGATGATAGTGATGTGGATCTCTTTGGTGAAGAGACTGAAGAGGAGAAGAAGGCTGCCGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAGGAGTCTGGCAAGTCATCAGTTCTATTCGATATTGAGCCGTGGGATGATGAAACAGACATGAAAAAGCTTGAGGAGGCAGTAAGGAGCATTGAGGTTGAGAGCTTACATTGGGGAGCATCTAAACTAGTACCTGCTGGGTATGGCATAAAGAAGTTGACAATTATGCTTGCAATTGTTGATGACCTCGTTTCTGTGGACTCTCTGATTGAAGAACGTCTTACCGTGGAACCTATTAATGAATATGTCCAGAGATATGACATTGTTGCCTTCAATAAAATATGTAAGATTGATGCACTGCTGAGTTGGAGTGGTTTGAAGATCTTTCTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

259

Amino Acids

28.24

Weight (kDa)

4.66

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 155 - 242 1.8e-27 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 586
AccB7I CCANNNNNTGG 1 cut(s) 317
AciI CCGC 1 cut(s) 297
AclWI GGATC 2 cut(s) 280, 372
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 2 cut(s) 360, 405
AfaI GTAC 3 cut(s) 59, 173, 576
AfiI CCNNNNNNNGG 3 cut(s) 287, 314, 317
AgsI TTSAA 5 cut(s) 214, 324, 656, 718, 763
AhlI ACTAGT 1 cut(s) 571
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 5 cut(s) 32, 146, 419, 514, 549
AluI AGCT 5 cut(s) 32, 146, 419, 514, 549
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 2 cut(s) 280, 372
AlwNI CAGNNNCTG 2 cut(s) 35, 422
AoxI GGCC 2 cut(s) 37, 327
ApeKI GCWGC 8 cut(s) 32, 122, 185, 299, 335, 401, 416, 419
ApoI RAATTY 1 cut(s) 46
AseI ATTAAT 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 73, 386
AsuII TTCGAA 1 cut(s) 98
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 8 cut(s) 19, 109, 172, 286, 322, 388, 406, 428
BccI CCATC 2 cut(s) 148, 290
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BcoDI GTCTC 1 cut(s) 374
BcuI ACTAGT 1 cut(s) 571
BfaI CTAG 1 cut(s) 572
BfuAI ACCTGC 1 cut(s) 586
BglII AGATCT 1 cut(s) 765
BisI GCNGC 9 cut(s) 33, 123, 186, 297, 300, 336, 402, 417, 420
BlsI GCNGC 9 cut(s) 34, 124, 187, 298, 301, 337, 403, 418, 421
BmcAI AGTACT 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 675
BmsI GCATC 4 cut(s) 170, 440, 572, 727
BpmI CTGGAG 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 98
BpuEI CTTGAG 2 cut(s) 26, 536
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 485, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 314, 317
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 55, 319
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 485, 669
BseGI GGATG 2 cut(s) 109, 496
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 3 cut(s) 287, 314, 317
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 42, 737
BseNI ACTGG 2 cut(s) 55, 319
BseRI GAGGAG 2 cut(s) 404, 533
BseXI GCAGC 8 cut(s) 19, 109, 172, 286, 322, 388, 406, 428
BseYI CCCAGC 1 cut(s) 581
BsgI GTGCAG 2 cut(s) 171, 435
BshFI GGCC 2 cut(s) 39, 329
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 287, 314, 317
BsmAI GTCTC 1 cut(s) 374
BsmI GAATGC 2 cut(s) 11, 163
BsnI GGCC 2 cut(s) 39, 329
Bsp119I TTCGAA 1 cut(s) 98
Bsp143I GATC 3 cut(s) 272, 364, 765
BspACI CCGC 1 cut(s) 297
BspANI GGCC 2 cut(s) 39, 329
BspCNI CTCAG 2 cut(s) 41, 738
BspLI GGNNCC 1 cut(s) 675
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 280, 372
BspT104I TTCGAA 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 55, 319
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 485, 669
BssMI GATC 3 cut(s) 272, 364, 765
Bst4CI ACNGT 2 cut(s) 115, 670
Bst6I CTCTTC 2 cut(s) 372, 381
BstBAI YACGTR 1 cut(s) 154
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 37, 120, 615
BstDEI CTNAG 3 cut(s) 28, 194, 746
BstDSI CCRYGG 2 cut(s) 485, 669
BstF5I GGATG 2 cut(s) 109, 496
BstKTI GATC 3 cut(s) 275, 367, 768
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 272, 364, 765
BstMWI GCNNNNNNNGC 2 cut(s) 335, 428
BstNSI RCATGY 1 cut(s) 122
BstV1I GCAGC 8 cut(s) 19, 109, 172, 286, 322, 388, 406, 428
BstX2I RGATCY 3 cut(s) 272, 364, 765
BstYI RGATCY 3 cut(s) 272, 364, 765
BsuRI GGCC 2 cut(s) 39, 329
BtgI CCRYGG 2 cut(s) 485, 669
BtsCI GGATG 2 cut(s) 109, 496
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 2 cut(s) 111, 740
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 3 cut(s) 37, 120, 615
CaiI CAGNNNCTG 2 cut(s) 35, 422
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 3 cut(s) 58, 172, 575
CviAII CATG 3 cut(s) 17, 119, 505
CviQI GTAC 3 cut(s) 58, 172, 575
DdeI CTNAG 3 cut(s) 28, 194, 746
DpnI GATC 3 cut(s) 274, 366, 767
DpnII GATC 3 cut(s) 272, 364, 765
Eam1104I CTCTTC 2 cut(s) 372, 381
EarI CTCTTC 2 cut(s) 372, 381
Eco147I AGGCCT 1 cut(s) 329
Eco32I GATATC 2 cut(s) 109, 220
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 360, 405
EcoRV GATATC 2 cut(s) 109, 220
EcoT22I ATGCAT 1 cut(s) 163
FaeI CATG 3 cut(s) 20, 122, 508
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FatI CATG 3 cut(s) 16, 118, 504
Fnu4HI GCNGC 9 cut(s) 33, 123, 186, 297, 300, 336, 402, 417, 420
FokI GGATG 2 cut(s) 116, 503
Fsp4HI GCNGC 9 cut(s) 33, 123, 186, 297, 300, 336, 402, 417, 420
FspBI CTAG 1 cut(s) 572
GluI GCNGC 9 cut(s) 33, 123, 186, 297, 300, 336, 402, 417, 420
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 2 cut(s) 39, 329
HapII CCGG 1 cut(s) 84
Hin1II CATG 3 cut(s) 20, 122, 508
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 3 cut(s) 136, 449, 644
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 73, 386
Hpy166II GTNNAC 2 cut(s) 603, 643
Hpy188I TCNGA 1 cut(s) 651
Hpy188III TCNNGA 3 cut(s) 43, 243, 694
Hpy8I GTNNAC 2 cut(s) 603, 643
HpyAV CCTTC 5 cut(s) 94, 242, 391, 439, 724
HpyCH4III ACNGT 2 cut(s) 115, 670
HpyCH4IV ACGT 3 cut(s) 153, 412, 661
HpyCH4V TGCA 7 cut(s) 11, 118, 161, 188, 416, 617, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 428
HpyF3I CTNAG 3 cut(s) 28, 194, 746
HpySE526I ACGT 3 cut(s) 153, 412, 661
Hsp92II CATG 3 cut(s) 20, 122, 508
Kzo9I GATC 3 cut(s) 272, 364, 765
LmnI GCTCC 3 cut(s) 283, 531, 560
Lsp1109I GCAGC 8 cut(s) 19, 109, 172, 286, 322, 388, 406, 428
LweI GCATC 4 cut(s) 170, 440, 572, 727
MaeI CTAG 1 cut(s) 572
MaeII ACGT 3 cut(s) 153, 412, 661
MaeIII GTNAC 1 cut(s) 74
MalI GATC 3 cut(s) 274, 366, 767
MboI GATC 3 cut(s) 272, 364, 765
MboII GAAGA 7 cut(s) 353, 389, 398, 406, 419, 668, 775
MfeI CAATTG 2 cut(s) 264, 618
MflI RGATCY 3 cut(s) 272, 364, 765
MluCI AATT 4 cut(s) 46, 264, 606, 618
MlyI GAGTC 3 cut(s) 130, 458, 638
MmeI TCCRAC 2 cut(s) 335, 731
MnlI CCTC 9 cut(s) 50, 262, 318, 340, 382, 511, 514, 532, 641
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 4 cut(s) 32, 146, 299, 419
MspI CCGG 1 cut(s) 84
MunI CAATTG 2 cut(s) 264, 618
Mva1269I GAATGC 2 cut(s) 11, 163
MwoI GCNNNNNNNGC 2 cut(s) 335, 428
NdeII GATC 3 cut(s) 272, 364, 765
NlaIII CATG 3 cut(s) 20, 122, 508
NlaIV GGNNCC 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 98
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 329
PctI GAATGC 2 cut(s) 11, 163
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 9 cut(s) 34, 124, 187, 298, 301, 337, 403, 418, 421
PleI GAGTC 3 cut(s) 130, 457, 638
PpsI GAGTC 3 cut(s) 130, 457, 638
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 2 cut(s) 35, 422
PsuI RGATCY 3 cut(s) 272, 364, 765
PvuII CAGCTG 3 cut(s) 32, 146, 419
RsaI GTAC 3 cut(s) 59, 173, 576
RsaNI GTAC 3 cut(s) 58, 172, 575
RseI CAYNNNNRTG 1 cut(s) 166
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 9 cut(s) 33, 123, 186, 297, 300, 336, 402, 417, 420
Sau3AI GATC 3 cut(s) 272, 364, 765
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 3 cut(s) 130, 458, 638
SfaNI GCATC 4 cut(s) 170, 440, 572, 727
SfuI TTCGAA 1 cut(s) 98
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 41, 515
SmoI CTYRAG 2 cut(s) 41, 515
SpeI ACTAGT 1 cut(s) 571
SphI GCATGC 1 cut(s) 122
Sse9I AATT 4 cut(s) 46, 264, 606, 618
SseBI AGGCCT 1 cut(s) 329
SsiI CCGC 1 cut(s) 297
SspMI CTAG 1 cut(s) 572
StuI AGGCCT 1 cut(s) 329
TaaI ACNGT 2 cut(s) 115, 670
TaiI ACGT 3 cut(s) 156, 415, 664
TaqI TCGA 2 cut(s) 98, 474
TasI AATT 4 cut(s) 46, 264, 606, 618
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 2 cut(s) 118, 747
TseI GCWGC 8 cut(s) 32, 122, 185, 299, 335, 401, 416, 419
TspDTI ATGAA 3 cut(s) 510, 521, 699
TspRI CASTG 2 cut(s) 118, 747
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 1 cut(s) 46
XceI RCATGY 1 cut(s) 122
XspI CTAG 1 cut(s) 572
ZrmI AGTACT 1 cut(s) 59
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.