FvH4_7g00650

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003320
Physical Location & Seq
Forward (+)
1177830 .. 1179829
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00650.t2

Sequence Viewer

Length: 699 bp
ATGGCAGTTGCATTCCATGACATCAACTCGGCTGCTGGCCTCAAGAAATTGGACCAGTACTTGCTCACTCGCAGCTACATCACCGGCTATCAGGCGTCAAAGGATGATATCACCGTGCACGCTGCTCTCTCCAAGCCCCCATCATCTGAATATGTGAATGCATCCCGGTGGTACAACCACATTGCTGCCCTTCTTAGGATTTCGGGTGTTTCTGAAGAAGGCTGTGGTGTCACAATTGAGGGATGTGCTCCCATCACAGGGGAAGCAGTTGCCACTCCTCCCGCTGCTGATTCAAAGGCCTCTGCTGCTGAAGATGATGATGACAGTGATGTGGATCTCTTTGGTGAGGAGACCGAAGAGGAGAAGAAGGCTGCTGAAGAACGTGCAGCTGCTGTCAAGGCATCTACCAAAAAGAAAGAGTCTGGCAAGTCATCAGTTCTGCTTGATGTTAAGCCATGGGATGATGAAACAGACATGAAAAAGCTTGAGGAAGCAGTAAGGAGTGTCGAGGTTGAGGGCTTACACTGGGGAGCATCTAAACTTGTACCTGTTGGGTATGGCATAAAGAAGTTGACAATCATGCTTACAATTATTGATGACCTTGTTTCTGTGGACACTCTAATTGAGGAACGTCTTACCGCGGAACCTATCAATGAATATGTCCAGAGTTGTGACATTGTTGCATTCAATAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

233

Amino Acids

25.08

Weight (kDa)

4.63

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 145 - 232 1.6e-30 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 641
AciI CCGC 3 cut(s) 282, 639, 641
AclWI GGATC 1 cut(s) 342
AcuI CTGAAG 3 cut(s) 234, 330, 396
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 59, 173, 546
AfiI CCNNNNNNNGG 3 cut(s) 195, 257, 258
AgsI TTSAA 2 cut(s) 294, 688
AluBI AGCT 3 cut(s) 75, 389, 484
AluI AGCT 3 cut(s) 75, 389, 484
Alw21I GWGCWC 2 cut(s) 120, 250
Alw26I GTCTC 1 cut(s) 344
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 1 cut(s) 342
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 2 cut(s) 37, 297
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 9 cut(s) 32, 72, 122, 185, 284, 305, 371, 386, 389
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 73, 103, 356
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 120, 250
BbvI GCAGC 9 cut(s) 19, 84, 109, 172, 271, 292, 358, 376, 398
BccI CCATC 2 cut(s) 148, 260
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 344
BisI GCNGC 9 cut(s) 33, 73, 123, 186, 285, 306, 372, 387, 390
BlsI GCNGC 9 cut(s) 34, 74, 124, 187, 286, 307, 373, 388, 391
BmcAI AGTACT 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 166
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 170, 410, 542
BmuI ACTGGG 1 cut(s) 535
BpuEI CTTGAG 2 cut(s) 26, 506
BpuMI CCSGG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 455, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 257, 258
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 55, 530
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 455, 639
BseGI GGATG 4 cut(s) 109, 161, 248, 466
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 195, 257, 258
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 55, 530
BseRI GAGGAG 3 cut(s) 267, 362, 374
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 9 cut(s) 19, 84, 109, 172, 271, 292, 358, 376, 398
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 641
BshFI GGCC 2 cut(s) 39, 299
BsiHKAI GWGCWC 2 cut(s) 120, 250
BsiSI CCGG 2 cut(s) 84, 166
BslI CCNNNNNNNGG 3 cut(s) 195, 257, 258
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 3 cut(s) 11, 163, 683
BsnI GGCC 2 cut(s) 39, 299
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 120, 250
Bsp143I GATC 1 cut(s) 334
Bsp19I CCATGG 1 cut(s) 455
BspACI CCGC 3 cut(s) 282, 639, 641
BspANI GGCC 2 cut(s) 39, 299
BspFNI CGCG 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 645
BspPI GGATC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 55, 530
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 455, 639
BssMI GATC 1 cut(s) 334
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 455
Bst4CI ACNGT 2 cut(s) 115, 326
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 37, 120
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 2 cut(s) 455, 639
BstF5I GGATG 4 cut(s) 109, 161, 248, 466
BstFNI CGCG 1 cut(s) 641
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 305, 398
BstSCI CCNGG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 9 cut(s) 19, 84, 109, 172, 271, 292, 358, 376, 398
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 2 cut(s) 39, 299
BtgI CCRYGG 2 cut(s) 455, 639
BtsCI GGATG 4 cut(s) 109, 161, 248, 466
BtsIMutI CAGTG 2 cut(s) 331, 523
Cac8I GCNNGC 2 cut(s) 37, 120
CaiI CAGNNNCTG 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 52
Cfr42I CCGCGG 1 cut(s) 642
CseI GACGC 1 cut(s) 84
Csp6I GTAC 3 cut(s) 58, 172, 545
CviAII CATG 4 cut(s) 17, 456, 475, 580
CviQI GTAC 3 cut(s) 58, 172, 545
DdeI CTNAG 1 cut(s) 194
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 344
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 3 cut(s) 234, 330, 396
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 455
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 4 cut(s) 20, 459, 478, 583
FaiI YATR 9 cut(s) 18, 153, 457, 476, 558, 563, 581, 660, 697
FatI CATG 4 cut(s) 16, 455, 474, 579
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 9 cut(s) 33, 73, 123, 186, 285, 306, 372, 387, 390
FokI GGATG 4 cut(s) 116, 148, 255, 473
Fsp4HI GCNGC 9 cut(s) 33, 73, 123, 186, 285, 306, 372, 387, 390
GluI GCNGC 9 cut(s) 33, 73, 123, 186, 285, 306, 372, 387, 390
HaeIII GGCC 2 cut(s) 39, 299
HapII CCGG 2 cut(s) 84, 166
HgaI GACGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 4 cut(s) 20, 459, 478, 583
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 290, 419
HpaII CCGG 2 cut(s) 84, 166
HphI GGTGA 3 cut(s) 73, 103, 356
Hpy166II GTNNAC 3 cut(s) 118, 573, 613
Hpy188I TCNGA 2 cut(s) 148, 214
Hpy188III TCNNGA 2 cut(s) 43, 664
Hpy8I GTNNAC 3 cut(s) 118, 573, 613
HpyAV CCTTC 3 cut(s) 200, 212, 361
HpyCH4III ACNGT 2 cut(s) 115, 326
HpyCH4IV ACGT 2 cut(s) 382, 631
HpyCH4V TGCA 5 cut(s) 11, 118, 161, 386, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 398
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 2 cut(s) 382, 631
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 4 cut(s) 20, 459, 478, 583
KspI CCGCGG 1 cut(s) 642
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 253, 530
Lsp1109I GCAGC 9 cut(s) 19, 84, 109, 172, 271, 292, 358, 376, 398
LweI GCATC 3 cut(s) 170, 410, 542
MaeII ACGT 2 cut(s) 382, 631
MaeIII GTNAC 2 cut(s) 229, 671
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 5 cut(s) 227, 323, 368, 376, 389
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 120, 250
MluCI AATT 4 cut(s) 47, 234, 588, 621
MlyI GAGTC 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 3 cut(s) 284, 389, 641
MspI CCGG 2 cut(s) 84, 166
MspR9I CCNGG 1 cut(s) 166
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 3 cut(s) 11, 163, 683
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 2 cut(s) 305, 398
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 455
NdeII GATC 1 cut(s) 334
NlaIII CATG 4 cut(s) 20, 459, 478, 583
NlaIV GGNNCC 1 cut(s) 645
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 229, 671
NsiI ATGCAT 1 cut(s) 163
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 3 cut(s) 11, 163, 683
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 9 cut(s) 34, 74, 124, 187, 286, 307, 373, 388, 391
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 59, 173, 546
RsaNI GTAC 3 cut(s) 58, 172, 545
RseI CAYNNNNRTG 1 cut(s) 166
SacII CCGCGG 1 cut(s) 642
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 9 cut(s) 33, 73, 123, 186, 285, 306, 372, 387, 390
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 166
SduI GDGCHC 2 cut(s) 120, 250
SetI ASST 9 cut(s) 77, 385, 391, 486, 513, 550, 603, 634, 649
SfaNI GCATC 3 cut(s) 170, 410, 542
Sfr303I CCGCGG 1 cut(s) 642
SgrBI CCGCGG 1 cut(s) 642
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 41, 485
SmoI CTYRAG 2 cut(s) 41, 485
Sse9I AATT 4 cut(s) 47, 234, 588, 621
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 3 cut(s) 282, 639, 641
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 164
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 115, 326
TaiI ACGT 2 cut(s) 385, 634
TaqI TCGA 1 cut(s) 507
TaqII GACCGA 1 cut(s) 368
TasI AATT 4 cut(s) 47, 234, 588, 621
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 2 cut(s) 331, 530
TseFI GTSAC 2 cut(s) 229, 671
TseI GCWGC 9 cut(s) 32, 72, 122, 185, 284, 305, 371, 386, 389
Tsp45I GTSAC 2 cut(s) 229, 671
TspDTI ATGAA 3 cut(s) 480, 491, 669
TspRI CASTG 2 cut(s) 331, 530
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 59
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.