MD02G1316000.v1.1

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003320
Physical Location & Seq
Reverse (-)
37065316 .. 37067354
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1316000.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGCAGTCACATTCTTTGACGTCAACTCAGCCTCCGGCCTGAAGAAACTCGATGACTACTTGCTTGCTCGCAGTTACATCACTGGATATCAGGCTTCCAAAGATGATATCACTGTGTATGCAGCTCTTTCAAAGGCCCCTTCTTCCGAATTTGTGAATGTGTCTAGGTGGTACAACCACATTGCTGCTCTTCTTAGGATTTTTGGTGTAACCGGAGAAGGATGTGGTGTCACTATTGAGGGATCTGCTCCTATCACCGGGGAAGCAATTGCTACACCGCCTGTAGCTGATACAAAGGCCTCGGCTGCTGAGGATGACGATGATGACGATGTTGATCTCTTTGGGGAAGAGACCGAAGAGGAGAAGAAGGCTGCCGAAGAACGTGCAGCTTCTATCAAGGCATCAACAAAGAAGAAAGAGTCTGGCAAGTCGTCGGTTCTGTTGGATGTGAAGCCATGGGATGATGAAACTGACATGAAAAAGCTTGAGGAGGCTGTAAGAAGTGTCCACTTGGAAGGCTTGCATTGGGGAGCATCCAAACTTGTAGCTGTTGGGTACGGGATTAAGAAGTTGCAAATAATGATGACAATAGTTGATGACCTGGTCTCTGTTGACACTCTCATTGAGGAACATCTTACCGTCGAACCCATTAATGAGTATGTTCAGAGTTGTGACATCGTAGCCTTCAACAAAATATGTAAGATTCGAACTTGCAGAGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF1_GNE

Protein Analysis

242

Amino Acids

26.3

Weight (kDa)

4.67

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 145 - 232 1.1e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AciI CCGC 1 cut(s) 278
AclWI GGATC 1 cut(s) 250
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 2 cut(s) 173, 557
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 2 cut(s) 132, 688
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 5 cut(s) 125, 287, 389, 484, 548
AluI AGCT 5 cut(s) 125, 287, 389, 484, 548
Alw26I GTCTC 2 cut(s) 344, 610
AlwI GGATC 1 cut(s) 250
AoxI GGCC 3 cut(s) 37, 135, 297
ApeKI GCWGC 5 cut(s) 122, 185, 305, 371, 386
ApoI RAATTY 1 cut(s) 149
AseI ATTAAT 1 cut(s) 651
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 1 cut(s) 247
AsuII TTCGAA 1 cut(s) 706
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 5 cut(s) 134, 172, 292, 358, 398
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 602
BcnI CCSGG 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 344, 610
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 5 cut(s) 123, 186, 306, 372, 387
BlsI GCNGC 5 cut(s) 124, 187, 307, 373, 388
Bme1390I CCNGG 2 cut(s) 259, 602
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 259, 602
BmsI GCATC 2 cut(s) 410, 542
Bpu10I CCTNAGC 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 2 cut(s) 344, 610
BsaJI CCNNGG 3 cut(s) 258, 300, 455
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 3 cut(s) 258, 300, 455
BseGI GGATG 5 cut(s) 227, 319, 451, 466, 533
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 42, 300
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 2 cut(s) 374, 503
BseXI GCAGC 5 cut(s) 134, 172, 292, 358, 398
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 3 cut(s) 39, 137, 299
BsiSI CCGG 3 cut(s) 36, 213, 258
BslI CCNNNNNNNGG 1 cut(s) 257
BsmAI GTCTC 2 cut(s) 344, 610
BsnI GGCC 3 cut(s) 39, 137, 299
Bso31I GGTCTC 2 cut(s) 344, 610
Bsp119I TTCGAA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 242, 334
Bsp19I CCATGG 1 cut(s) 455
BspACI CCGC 1 cut(s) 278
BspANI GGCC 3 cut(s) 39, 137, 299
BspCNI CTCAG 2 cut(s) 41, 301
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 250
BspQI GCTCTTC 1 cut(s) 195
BspT104I TTCGAA 1 cut(s) 706
BspTNI GGTCTC 2 cut(s) 344, 610
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 3 cut(s) 258, 300, 455
BssMI GATC 2 cut(s) 242, 334
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 115, 640
Bst6I CTCTTC 3 cut(s) 195, 342, 351
BstACI GRCGYC 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 66, 70, 521
BstDEI CTNAG 3 cut(s) 28, 194, 309
BstDSI CCRYGG 1 cut(s) 455
BstF5I GGATG 5 cut(s) 227, 319, 451, 466, 533
BstKTI GATC 2 cut(s) 245, 337
BstMAI GTCTC 2 cut(s) 344, 610
BstMBI GATC 2 cut(s) 242, 334
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 1 cut(s) 602
BstSCI CCNGG 2 cut(s) 257, 600
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 5 cut(s) 134, 172, 292, 358, 398
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 3 cut(s) 39, 137, 299
BtgI CCRYGG 1 cut(s) 455
BtsCI GGATG 5 cut(s) 227, 319, 451, 466, 533
BtsIMutI CAGTG 2 cut(s) 81, 111
Cac8I GCNNGC 3 cut(s) 66, 70, 521
Cfr13I GGNCC 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 2 cut(s) 172, 556
CviAII CATG 2 cut(s) 456, 475
CviQI GTAC 2 cut(s) 172, 556
DdeI CTNAG 3 cut(s) 28, 194, 309
DpnI GATC 2 cut(s) 244, 336
DpnII GATC 2 cut(s) 242, 334
Eam1104I CTCTTC 3 cut(s) 195, 342, 351
EarI CTCTTC 3 cut(s) 195, 342, 351
Eco130I CCWWGG 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 2 cut(s) 344, 610
Eco32I GATATC 2 cut(s) 89, 109
Eco57I CTGAAG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 600
EcoRV GATATC 2 cut(s) 89, 109
EcoT14I CCWWGG 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 2 cut(s) 459, 478
FaiI YATR 6 cut(s) 120, 457, 476, 660, 697, 724
FatI CATG 2 cut(s) 455, 474
Fnu4HI GCNGC 5 cut(s) 123, 186, 306, 372, 387
FokI GGATG 5 cut(s) 234, 326, 458, 473, 520
Fsp4HI GCNGC 5 cut(s) 123, 186, 306, 372, 387
FspBI CTAG 1 cut(s) 165
GluI GCNGC 5 cut(s) 123, 186, 306, 372, 387
HaeIII GGCC 3 cut(s) 39, 137, 299
HapII CCGG 3 cut(s) 36, 213, 258
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 459, 478
HincII GTYRAC 2 cut(s) 25, 613
HindII GTYRAC 2 cut(s) 25, 613
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 419, 703
HpaII CCGG 3 cut(s) 36, 213, 258
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 3 cut(s) 25, 508, 613
Hpy188I TCNGA 2 cut(s) 148, 666
Hpy8I GTNNAC 3 cut(s) 25, 508, 613
Hpy99I CGWCG 2 cut(s) 436, 644
HpyAV CCTTC 5 cut(s) 150, 212, 361, 509, 694
HpyCH4III ACNGT 2 cut(s) 115, 640
HpyCH4IV ACGT 2 cut(s) 21, 382
HpyCH4V TGCA 5 cut(s) 122, 386, 523, 574, 714
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 28, 194, 309
HpySE526I ACGT 2 cut(s) 21, 382
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 459, 478
Kzo9I GATC 2 cut(s) 242, 334
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 2 cut(s) 253, 530
Lsp1109I GCAGC 5 cut(s) 134, 172, 292, 358, 398
LweI GCATC 2 cut(s) 410, 542
MabI ACCWGGT 1 cut(s) 600
MaeI CTAG 1 cut(s) 165
MaeII ACGT 2 cut(s) 21, 382
MaeIII GTNAC 5 cut(s) 7, 74, 208, 229, 671
MalI GATC 2 cut(s) 244, 336
MboI GATC 2 cut(s) 242, 334
MboII GAAGA 8 cut(s) 55, 135, 182, 359, 368, 376, 389, 424
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 1 cut(s) 242
MluCI AATT 2 cut(s) 149, 267
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 8 cut(s) 43, 232, 304, 310, 352, 481, 484, 619
MseI TTAA 2 cut(s) 564, 651
MspI CCGG 3 cut(s) 36, 213, 258
MspR9I CCNGG 2 cut(s) 259, 602
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 259
NcoI CCATGG 1 cut(s) 455
NdeII GATC 2 cut(s) 242, 334
NlaIII CATG 2 cut(s) 459, 478
NlaIV GGNNCC 1 cut(s) 138
NmeAIII GCCGAG 1 cut(s) 281
NmuCI GTSAC 3 cut(s) 7, 229, 671
NspV TTCGAA 1 cut(s) 706
PceI AGGCCT 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 1 cut(s) 703
PflFI GACNNNGTC 1 cut(s) 602
PkrI GCNGC 5 cut(s) 124, 187, 307, 373, 388
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PshBI ATTAAT 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 1 cut(s) 136
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 602
RsaI GTAC 2 cut(s) 173, 557
RsaNI GTAC 2 cut(s) 172, 556
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 564, 651
SatI GCNGC 5 cut(s) 123, 186, 306, 372, 387
Sau3AI GATC 2 cut(s) 242, 334
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 259, 602
SetI ASST 9 cut(s) 24, 127, 170, 289, 385, 391, 486, 550, 603
SexAI ACCWGGT 1 cut(s) 600
SfaNI GCATC 2 cut(s) 410, 542
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 2 cut(s) 149, 267
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 1 cut(s) 278
SspMI CTAG 1 cut(s) 165
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 257, 600
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 115, 640
TaiI ACGT 2 cut(s) 24, 385
TaqI TCGA 3 cut(s) 51, 642, 706
TaqII GACCGA 1 cut(s) 368
TasI AATT 2 cut(s) 149, 267
TfiI GAWTC 1 cut(s) 703
Tru1I TTAA 2 cut(s) 564, 651
Tru9I TTAA 2 cut(s) 564, 651
TscAI CASTG 2 cut(s) 88, 118
TseFI GTSAC 3 cut(s) 7, 229, 671
TseI GCWGC 5 cut(s) 122, 185, 305, 371, 386
Tsp45I GTSAC 3 cut(s) 7, 229, 671
TspDTI ATGAA 2 cut(s) 480, 491
TspRI CASTG 2 cut(s) 88, 118
Tth111I GACNNNGTC 1 cut(s) 602
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 1 cut(s) 149
XspI CTAG 1 cut(s) 165
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.