pycom06g13290

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004286
Physical Location & Seq
Forward (+)
18327101 .. 18329102
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13290.7

Sequence Viewer

Length: 1005 bp
ATGCGCCAACCGACCATGGTTTCAACGAGGCGAAGTGGATCCCTCTCCGGGAACAACAGCAAACGATCGTCGTCTTCCGAGGACAAGCCACCGTCGCCGAAACGACCGAAGGCGGATAATGGTAGTGCTTCGGAGAAAGCGACTCCTGGGGTGGAGAATTCGAAGGAGCTGTGCACGCTTCCGCCGGCGGCGGCTGCCGATCCCGGAGAATGTGGTCCCGAAGATGCGCCGGTCGCCGGAGACGGCGTGCCTTCTGGAAAGACCGAAGCTGCTGCGCCAGCTGTGGCGGTGACGACACCGATCGCCGAAGGGTCTTCGCCGGTGGTGGAAAAGCAACCGAGGAGTTCCTGGAGTTTCTATCAGAAGCAGAACCTGAGTATCGATACGCCTTGGTGCAAGCTTTTGTCACAGTCTGCACTGAATGTAAATATTTCTGTTAGTGCAGTAACATTCACAATTGGTGCAAGCAGACAGTGCAATTTTGCTCTGAAGGATCATACCATTAGTGGGGCTTTGTGCAAGATCAAGCGCACACAGCGTGAGGGCAGTGCTGTAGCAGTTCTTGAAAGTACGGGAAGCAAGGGATCAGTGCAAGTAAATGGGACAAATGTCAAGAGAGGCAACAGTTGCATGCTTAGCCCGGGTGATGAAGTTGTCTTTGGTTCAATGGGCAACCATGCTTATATTTTTCAGCTACTGCTGACTGAGGCTGCAGTTAAGGGTGCAGAGGTTCAAGGCAGTATTGGAAAATTTTTGCATCTTGAAAGGAGGGCAGGATCGATCCTTCAGCTGTGGCTGGGGCTTCCATACTGGCTTCTCTTAGCACGAGGGCAGAACAATCACGATGGAAGTCCGCAGCTCAGACCACCAGTAAAGTTCACCCCGGTGCTGAGGTACCAGCTCAGTCTGTCATTCAAGATGATACAGAAATTGAGCTTGATGGTCTCGAAAGCAGCTCAACTCCAAATAGAGGGACTGACAAGGCTGAAGATATTGGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

335

Amino Acids

35.54

Weight (kDa)

9.51

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 894
AccB1I GGYRCC 1 cut(s) 894
AciI CCGC 6 cut(s) 113, 182, 188, 191, 287, 854
AclWI GGATC 7 cut(s) 33, 46, 194, 501, 592, 775, 784
AcsI RAATTY 2 cut(s) 157, 749
AcuI CTGAAG 2 cut(s) 509, 770
AdeI CACNNNGTG 1 cut(s) 539
AfaI GTAC 2 cut(s) 571, 896
AfiI CCNNNNNNNGG 4 cut(s) 48, 236, 507, 970
AgsI TTSAA 6 cut(s) 24, 566, 666, 734, 764, 916
AjnI CCWGG 2 cut(s) 145, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AleI CACNNNNGTG 1 cut(s) 884
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 2 cut(s) 234, 949
Alw44I GTGCAC 1 cut(s) 172
AlwI GGATC 7 cut(s) 33, 46, 194, 501, 592, 775, 784
AlwNI CAGNNNCTG 2 cut(s) 373, 697
Ama87I CYCGRG 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 172
ApeKI GCWGC 6 cut(s) 194, 269, 272, 710, 856, 953
ApoI RAATTY 2 cut(s) 157, 749
Asp718I GGTACC 1 cut(s) 894
AspLEI GCGC 4 cut(s) 6, 229, 277, 531
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 5 cut(s) 49, 204, 641, 642, 884
AsuHPI GGTGA 3 cut(s) 301, 656, 871
AsuII TTCGAA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 640
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BamHI GGATCC 1 cut(s) 38
BanI GGYRCC 1 cut(s) 894
BauI CACGAG 1 cut(s) 825
BbsI GAAGAC 2 cut(s) 66, 306
Bbv12I GWGCWC 1 cut(s) 176
BbvCI CCTCAGC 1 cut(s) 890
BbvI GCAGC 6 cut(s) 181, 256, 259, 697, 868, 965
BccI CCATC 2 cut(s) 839, 934
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 2 cut(s) 147, 349
BcnI CCSGG 5 cut(s) 49, 204, 641, 642, 884
BcoDI GTCTC 2 cut(s) 234, 949
BfmI CTRYAG 2 cut(s) 552, 711
BisI GCNGC 8 cut(s) 189, 192, 195, 270, 273, 711, 857, 954
BlpI GCTNAGC 1 cut(s) 635
BlsI GCNGC 8 cut(s) 190, 193, 196, 271, 274, 712, 858, 955
Bme1390I CCNGG 7 cut(s) 49, 147, 204, 349, 641, 642, 884
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 640
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 3 cut(s) 40, 217, 896
BmrFI CCNGG 7 cut(s) 49, 147, 204, 349, 641, 642, 884
BmsI GCATC 2 cut(s) 214, 766
BoxI GACNNNNGTC 1 cut(s) 608
BpiI GAAGAC 2 cut(s) 66, 306
BpmI CTGGAG 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 890
Bpu1102I GCTNAGC 1 cut(s) 635
Bpu14I TTCGAA 1 cut(s) 161
BpuMI CCSGG 5 cut(s) 49, 204, 641, 642, 884
Bsa29I ATCGAT 2 cut(s) 381, 779
BsaI GGTCTC 1 cut(s) 949
BsaJI CCNNGG 7 cut(s) 15, 78, 146, 338, 389, 640, 882
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 236, 507, 970
Bse118I RCCGGY 3 cut(s) 184, 229, 319
Bse1I ACTGG 2 cut(s) 815, 869
BseBI CCWGG 2 cut(s) 147, 349
BseCI ATCGAT 2 cut(s) 381, 779
BseDI CCNNGG 7 cut(s) 15, 78, 146, 338, 389, 640, 882
BseLI CCNNNNNNNGG 4 cut(s) 48, 236, 507, 970
BseMII CTCAG 5 cut(s) 365, 696, 874, 881, 916
BseNI ACTGG 2 cut(s) 815, 869
BseRI GAGGAG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 6 cut(s) 181, 256, 259, 697, 868, 965
BseYI CCCAGC 1 cut(s) 796
BsgI GTGCAG 3 cut(s) 399, 462, 744
Bsh1285I CGRYCG 4 cut(s) 68, 107, 234, 303
BshNI GGYRCC 1 cut(s) 894
BshVI ATCGAT 2 cut(s) 381, 779
BsiEI CGRYCG 4 cut(s) 68, 107, 234, 303
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 640
BsiSI CCGG 8 cut(s) 48, 185, 204, 230, 237, 320, 641, 884
BslFI GGGAC 3 cut(s) 201, 616, 987
BslI CCNNNNNNNGG 4 cut(s) 48, 236, 507, 970
BsmAI GTCTC 2 cut(s) 234, 949
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 3 cut(s) 201, 616, 987
Bso31I GGTCTC 1 cut(s) 949
BsoBI CYCGRG 1 cut(s) 640
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
Bsp1720I GCTNAGC 1 cut(s) 635
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 6 cut(s) 113, 182, 188, 191, 287, 854
BspCNI CTCAG 5 cut(s) 366, 697, 873, 882, 915
BspDI ATCGAT 2 cut(s) 381, 779
BspLI GGNNCC 3 cut(s) 40, 217, 896
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 7 cut(s) 33, 46, 194, 501, 592, 775, 784
BspT104I TTCGAA 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 894
BspTNI GGTCTC 1 cut(s) 949
BsrFI RCCGGY 3 cut(s) 184, 229, 319
BsrI ACTGG 2 cut(s) 815, 869
BssAI RCCGGY 3 cut(s) 184, 229, 319
BssECI CCNNGG 7 cut(s) 15, 78, 146, 338, 389, 640, 882
BssMI GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 2 cut(s) 15, 389
Bst2BI CACGAG 1 cut(s) 825
Bst2UI CCWGG 2 cut(s) 147, 349
Bst4CI ACNGT 4 cut(s) 93, 411, 474, 626
BstAPI GCANNNNNTGC 2 cut(s) 474, 627
BstBI TTCGAA 1 cut(s) 161
BstC8I GCNNGC 7 cut(s) 176, 186, 248, 279, 398, 466, 632
BstDEI CTNAG 7 cut(s) 374, 635, 705, 820, 860, 890, 902
BstDSI CCRYGG 1 cut(s) 15
BstHHI GCGC 4 cut(s) 6, 229, 277, 531
BstKTI GATC 9 cut(s) 41, 68, 202, 303, 496, 525, 587, 779, 783
BstMAI GTCTC 2 cut(s) 234, 949
BstMBI GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
BstMCI CGRYCG 4 cut(s) 68, 107, 234, 303
BstMWI GCNNNNNNNGC 9 cut(s) 94, 175, 194, 233, 278, 474, 535, 627, 636
BstNI CCWGG 2 cut(s) 147, 349
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 608
BstSCI CCNGG 7 cut(s) 47, 145, 202, 347, 639, 640, 882
BstSFI CTRYAG 2 cut(s) 552, 711
BstSLI GKGCMC 1 cut(s) 176
BstV1I GCAGC 6 cut(s) 181, 256, 259, 697, 868, 965
BstV2I GAAGAC 2 cut(s) 66, 306
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
Bsu15I ATCGAT 2 cut(s) 381, 779
BsuTUI ATCGAT 2 cut(s) 381, 779
BtgI CCRYGG 1 cut(s) 15
BtsI GCAGTG 1 cut(s) 553
BtsIMutI CAGTG 4 cut(s) 416, 479, 553, 594
Cac8I GCNNGC 7 cut(s) 176, 186, 248, 279, 398, 466, 632
CaiI CAGNNNCTG 2 cut(s) 373, 697
CfoI GCGC 4 cut(s) 6, 229, 277, 531
Cfr10I RCCGGY 3 cut(s) 184, 229, 319
Cfr13I GGNCC 1 cut(s) 215
Cfr9I CCCGGG 1 cut(s) 640
ClaI ATCGAT 2 cut(s) 381, 779
Csp6I GTAC 2 cut(s) 570, 895
CviAII CATG 3 cut(s) 16, 631, 677
CviQI GTAC 2 cut(s) 570, 895
DdeI CTNAG 7 cut(s) 374, 635, 705, 820, 860, 890, 902
DpnI GATC 9 cut(s) 40, 67, 201, 302, 495, 524, 586, 778, 782
DpnII GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
DraIII CACNNNGTG 1 cut(s) 539
EciI GGCGGA 2 cut(s) 128, 171
Eco130I CCWWGG 2 cut(s) 15, 389
Eco31I GGTCTC 1 cut(s) 949
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 509, 770
Eco88I CYCGRG 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 145, 347
EcoT14I CCWWGG 2 cut(s) 15, 389
ErhI CCWWGG 2 cut(s) 15, 389
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 3 cut(s) 19, 634, 680
FaiI YATR 6 cut(s) 17, 498, 632, 678, 684, 808
FaqI GGGAC 3 cut(s) 201, 616, 987
FatI CATG 3 cut(s) 15, 630, 676
Fnu4HI GCNGC 8 cut(s) 189, 192, 195, 270, 273, 711, 857, 954
Fsp4HI GCNGC 8 cut(s) 189, 192, 195, 270, 273, 711, 857, 954
GlaI GCGC 4 cut(s) 5, 228, 276, 530
GluI GCNGC 8 cut(s) 189, 192, 195, 270, 273, 711, 857, 954
GsaI CCCAGC 1 cut(s) 800
GsuI CTGGAG 1 cut(s) 370
HapII CCGG 8 cut(s) 48, 185, 204, 230, 237, 320, 641, 884
HhaI GCGC 4 cut(s) 6, 229, 277, 531
Hin1II CATG 3 cut(s) 19, 634, 680
Hin6I GCGC 4 cut(s) 4, 227, 275, 529
HinP1I GCGC 4 cut(s) 4, 227, 275, 529
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 1 cut(s) 142
HpaII CCGG 8 cut(s) 48, 185, 204, 230, 237, 320, 641, 884
HphI GGTGA 3 cut(s) 301, 656, 871
Hpy166II GTNNAC 2 cut(s) 174, 879
Hpy188I TCNGA 5 cut(s) 79, 133, 363, 489, 863
Hpy188III TCNNGA 8 cut(s) 218, 255, 563, 613, 761, 842, 916, 946
Hpy8I GTNNAC 2 cut(s) 174, 879
Hpy99I CGWCG 2 cut(s) 73, 97
HpyAV CCTTC 6 cut(s) 103, 157, 261, 302, 484, 794
HpyCH4III ACNGT 4 cut(s) 93, 411, 474, 626
HpyF10VI GCNNNNNNNGC 9 cut(s) 94, 175, 194, 233, 278, 474, 535, 627, 636
HpyF3I CTNAG 7 cut(s) 374, 635, 705, 820, 860, 890, 902
Hsp92II CATG 3 cut(s) 19, 634, 680
HspAI GCGC 4 cut(s) 4, 227, 275, 529
KpnI GGTACC 1 cut(s) 898
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Kzo9I GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
LmnI GCTCC 2 cut(s) 166, 997
Lsp1109I GCAGC 6 cut(s) 181, 256, 259, 697, 868, 965
LweI GCATC 2 cut(s) 214, 766
MaeIII GTNAC 3 cut(s) 289, 405, 445
MalI GATC 9 cut(s) 40, 67, 201, 302, 495, 524, 586, 778, 782
MboI GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
MboII GAAGA 4 cut(s) 66, 233, 306, 1000
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 5 cut(s) 157, 456, 478, 749, 929
MlyI GAGTC 1 cut(s) 136
MreI CGCCGGCG 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 184
MseI TTAA 1 cut(s) 717
MslI CAYNNNNRTG 1 cut(s) 884
MspA1I CMGCKG 2 cut(s) 281, 790
MspI CCGG 8 cut(s) 48, 185, 204, 230, 237, 320, 641, 884
MspR9I CCNGG 7 cut(s) 49, 147, 204, 349, 641, 642, 884
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 2 cut(s) 147, 349
MwoI GCNNNNNNNGC 9 cut(s) 94, 175, 194, 233, 278, 474, 535, 627, 636
NaeI GCCGGC 1 cut(s) 186
NciI CCSGG 5 cut(s) 49, 204, 641, 642, 884
NcoI CCATGG 1 cut(s) 15
NdeII GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
NgoMIV GCCGGC 1 cut(s) 184
NlaIII CATG 3 cut(s) 19, 634, 680
NlaIV GGNNCC 3 cut(s) 40, 217, 896
NmuCI GTSAC 2 cut(s) 289, 405
NspI RCATGY 1 cut(s) 634
NspV TTCGAA 1 cut(s) 161
OliI CACNNNNGTG 1 cut(s) 884
PaeI GCATGC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PdiI GCCGGC 1 cut(s) 186
PfoI TCCNGGA 3 cut(s) 47, 202, 347
PkrI GCNGC 8 cut(s) 190, 193, 196, 271, 274, 712, 858, 955
Ple19I CGATCG 2 cut(s) 68, 303
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 145, 347
PspFI CCCAGC 1 cut(s) 796
PspGI CCWGG 2 cut(s) 145, 347
PspN4I GGNNCC 3 cut(s) 40, 217, 896
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 715
PstNI CAGNNNCTG 2 cut(s) 373, 697
PsuI RGATCY 1 cut(s) 38
PvuI CGATCG 2 cut(s) 68, 303
PvuII CAGCTG 2 cut(s) 281, 790
RsaI GTAC 2 cut(s) 571, 896
RsaNI GTAC 2 cut(s) 570, 895
RseI CAYNNNNRTG 1 cut(s) 884
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 8 cut(s) 189, 192, 195, 270, 273, 711, 857, 954
Sau3AI GATC 9 cut(s) 38, 65, 199, 300, 493, 522, 584, 776, 780
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 136
ScrFI CCNGG 7 cut(s) 49, 147, 204, 349, 641, 642, 884
SduI GDGCHC 1 cut(s) 176
SfaNI GCATC 2 cut(s) 214, 766
SfcI CTRYAG 2 cut(s) 552, 711
SfuI TTCGAA 1 cut(s) 161
SgrAI CRCCGGYG 2 cut(s) 184, 319
SinI GGWCC 1 cut(s) 215
SmaI CCCGGG 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 884
SphI GCATGC 1 cut(s) 634
Sse9I AATT 5 cut(s) 157, 456, 478, 749, 929
SsiI CCGC 6 cut(s) 113, 182, 188, 191, 287, 854
SspI AATATT 1 cut(s) 430
StyD4I CCNGG 7 cut(s) 47, 145, 202, 347, 639, 640, 882
StyI CCWWGG 2 cut(s) 15, 389
TaaI ACNGT 4 cut(s) 93, 411, 474, 626
TaqI TCGA 4 cut(s) 161, 381, 779, 947
TaqII GACCGA 2 cut(s) 121, 278
TasI AATT 5 cut(s) 157, 456, 478, 749, 929
TauI GCSGC 2 cut(s) 191, 194
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 4 cut(s) 423, 479, 553, 594
TseFI GTSAC 2 cut(s) 289, 405
TseI GCWGC 6 cut(s) 194, 269, 272, 710, 856, 953
Tsp45I GTSAC 2 cut(s) 289, 405
TspDTI ATGAA 1 cut(s) 663
TspMI CCCGGG 1 cut(s) 640
TspRI CASTG 4 cut(s) 423, 479, 553, 594
VneI GTGCAC 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 157, 749
XceI RCATGY 1 cut(s) 634
XmaI CCCGGG 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.