Rw7G009560

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004286
Physical Location & Seq
Forward (+)
8921970 .. 8931743
9774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009560.1

Sequence Viewer

Length: 3747 bp
ATGGTTTCAACGAGGAGAAGTGGATCTCTCTCCGGCAATAACAGTAAAAGATCGTCGTCTTCCGACGACAACAAGCCTCAGTCGCCGAAGCGGCAGAAGGTGGATAACAATGGCGTTGGTTCGGAGAAAACGGAGAATTCGAAGGAGGTTTGTACGCCGGCCACGGCGGCGGCTGATCCCGGAGAATGCAGCGCCGCCGGCGAGGCTCAGACGGCCGGAGACGGCGCGACTTCTGTAAAGACTGAAGCTTCTGCACAAGCCGTGGCGGTGACCCCGCCGCCTGTGGCTGAAGGTGCTGAAGGGTCTTCGCCGGCAGCTGTGCTGGATAAGGCGACTTCCTTTTTGGCATGGAGTTCCTTTCAGAAGCAGAGCCTGAATTCGGGGAGTTTCGAGTTGACAACACCTTGGTGCAAGCTTCTGTCACAGTCTGGACAGAATCTAAACATTTCTATCACCACATCGATTTTTACAATTGGTTCGAGTAGAAGCAGCAATTTCCCTCTGAAGGATAATACCATTAGTGCGTGTCTGTGCAAGATCAAACGCGAACAGCGGGAGAGCGGTGCTGTAACGATTATAGAAAGTATGGGAAACAAGGGATCACTGCAAGTAAATGGGGCGCATGTGAAGAAAGGCAACAGCTGCGTGCTCAACTCAGGTGATGAAGTGGTATTTGGTTCTCTGGGGAACCATGCTTATATTTTTCAACTACTTGTGGCGGAGGGTGCGGTCAAGGGTGCAGAGGTTCAAAGTGGTGTTGGAAAATATCTGCACCTTGACAGGAGAGCCGGAGATCCTTCAGATGTGGATGGGGCTTCCATACTGGCTTCTCTTAGCATGAGACCAGAACTATCACGATGGAAGTCTGCGGCTCAGACCACCAATAAAGTCCATCCAGGGGCTGAAGTACCAGCTCAGTCTGTTATTCGAGAAGGTACAGAAGTTGAGCTTGAAGAACTCGAAGGCAGCTTGACCCCAAACAGAACAACTGACAAAACAGAAGAGATCGGAGGAATCAACAAGAGTCTTACTCCGGACTGCAACCCAGATTCGGGTGTAGAGGCAGACAATGTAAAATTCTCTGGGATGAATGATTTGCTAGGGCCTTTATTGAGGATGTTAGCACGAACACCTAGTTATAAACTGAAATTGAGCAAAGGTATCTGTAAACAGGTATTGGAAGAAAAAAATGAGTGGACGAGGGATGCGCTGCCAGCATCAACGTCAGGCATGTCTGTGCGGTGTGCTGTTTTTAAAGAAGGCATTCATGCAGCAATTCTTGAAGGCAATAGCATGGATGTTTCATTTGATGATTTCCCGTACTATTTGAGTGAGAATACGAAGAATGTGCTGATTGCAGCTTCATTTATACACTTGAAACATAAAGAACATGCAAAGTATACTACAGAGCTTACCACTGTGAATCCTCGAATATTGCTCTCTGGTCCTGCAGGGTCTGAGATATACCAGGAGATGTTGGCAAAGGCACTTGCTCAATATTTCGGGGCTAAGTTGCTCATATTTGATAGCCAATCACTTTTGGGTGGTTTGTCATCAAAGGAAGCCGAGCTGCCCAAGGATGGGTCTAATGGAGATAAAATAAGCACCTTGGCCAAACCAAGTCCTGTTTCCACAGACTTGTCTAAGAGCATTGATCCATCAGTTTCTGAGCTAGAGGCACCAAGGTTGGCGCCAAGCTCTTCAAATGGACCTTCATATGGCCTGGAATCCCAACCTAAGACGGAGATTGACTCCATCGCTACATCCTCAGGGACATCTAAGAATTACGTGTTTAAAATAGGTGACAGAGTAAAGTTTATTGGTTTATCTTCGGGCAACTTATATCCTACAACATCTTCACGGGGCCCAGTACCTGGAGCCCGAGGAGAGGTTGCGTTGGTTTTTGAGGACAATCCTTTGTCAAAAATTGGTGTGAGGTTTGATAAACAAGTATTTGATGGTGTTGATCTTGGGGGTCTTTGTAAAGGAAATGGATTCTTCTGCAATGCCTCTGATCTTCGTTCAGAAAACACAGGTGCGGATGATCTGGATAAATTACTGATCAATACATTGTTTGAGGCTGTACATAGTGAGAGCAGAGGTTCACCTTTCATTTTGTTCATGAAAGATGCGGAGAAGTCCCTTGTAGGAAATTCAGATTCATATTCCACATTCAGAGGCAGACTTGATAAGCTGCCTGATAATGTGGTTGTAATTGGCTCTCATACTCATACTGACAATCGCAAGGAGAAGTCGCATCCTGGTGGTCTACTTTTCACAAAGTTCGGTAGCAATCAAACTGCTCTTCTTGATTTGGCTTTTCCGGATAGTTTTGGAAGACTGCATGAAAGAGGGAAGGAAGTCCCCAAGGCAACAAAACTTCTGACAAAACTTTTTCCTAATAAAGTAACCATTCATATGCCACAGGATGAGGGACTTCTTGTCTCATGGAAACAGCAATTGGATCGTGATGCTGAAACCCTCAAAATGAAGGGAAATTTGAACCACTTGCGCACTGTTCTGGGTCGGTGTGGGATGGAATGTGAAGGACTTGATACGCTAAGCATCAAGGACCAAACACTTACAAATGAAAGTTCAGAGAAGGTAGTAGGATGGGCATTAAGCCATCATTTAATGGAGAATCCTGAAGCTGATCCAGAAACTAGAGTTGTTTTGTCTGCTGAGAGCATCAAGTACGGACTTGGAATCTTACAGTCTATCCAGAATGAAAATAAGAGCTTGAAGAAGTCACTTAAGGATGTTGTCACGGAAAATGAATTTGAGAAAAGGCTTTTGGCTGATGTTATCCCACCTACTGACATTGGAGTTACATTTGATGATATTGGCGCTCTTGAAAATGTGAAGGATACATTGAAAGAATTGGTGATGCTGCCTTTACAAAGGCCGGAGCTTTTCTGCAAAGGGCAATTAACCAAGCCTTGCAAAGGCATCCTTTTATTTGGTCCCCCGGGGACAGGCAAGACAATGCTGGCAAAGGCTGTGGCTACAGAAGCTGGTGCAAACTTCATCAACATATCCATGTCGAGCATCACATCTAAGTGGTTTGGTGAGGGTGAGAAGTATGTGAAAGCTGTTTTCTCTCTAGCTAGTAAAATTGCCCCTAGTGTCGTATTTGTTGATGAAGTTGACAGCATGTTGGGCCGGCGGGAAAATCCAGGGGAGCATGAGGCAATGCGCAAGATGAAAAATGAATTTATGGTAAATTGGGATGGGCTACGAACAAAAGAAGCAGAACGAGTTCTGGTACTTGCAGCCACAAATAGGCCTTTTGACCTTGATGAAGCTGTCATTCGAAGGCTGCCCCGAAGGTTGATGGTAAATTTGCCAGATGCTCCCAATAGGGCAAAGATATTAAGAGTCATATTGGCAAAAGAGGACTTATCTCCCGATGTTGATTTCGAGGCAATTGCGAGCATGTCAGATGGATATTCTGGAAGTGACCTTAAAAATCTATGCGTGGCTGCTGCACACCGTCCAATTAAAGAGATTTTGGAAAAGGAAAAGAAGGATCGTGCTGCAGCTATAGCAGAAGGTAAACCTATTCCAGCATTGAGTAGCAGTGGCGATGTTCGGCCTGTGAACATGGATGATTTTAAATATGCTCATGAACAGGTATGTGCCAGTGTATCATCGGAGTCTGTCAACATGACAGAGCTCTTACAATGGAATGAACTATATGGAGAAGGGGGTTCTAGAAGAAAGAAGGCCCTTAGTTACTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

1248

Amino Acids

134.8

Weight (kDa)

6.05

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 157 - 226 4.4e-06 FHA domain
DUF7751 PF24933 846 - 905 1.4e-18 Domain of unknown function (DUF7751)
AAA PF00004 984 - 1114 1.4e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 1137 - 1173 4e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
Acc16I TGCGCA 2 cut(s) 2513, 3197
AccB1I GGYRCC 2 cut(s) 1678, 1690
AccB7I CCANNNNNTGG 1 cut(s) 1874
AccBSI CCGCTC 1 cut(s) 561
AccI GTMKAC 2 cut(s) 1400, 2269
AccII CGCG 2 cut(s) 227, 546
AccIII TCCGGA 2 cut(s) 1033, 2323
AclWI GGATC 8 cut(s) 31, 170, 607, 788, 1649, 2472, 2648, 3537
AcoI YGGCCR 3 cut(s) 159, 213, 1611
AcsI RAATTY 8 cut(s) 136, 376, 1076, 2152, 2497, 2777, 3212, 3340
AcuI CTGAAG 7 cut(s) 264, 309, 318, 524, 783, 924, 2667
AcyI GRCGYC 1 cut(s) 1691
AfaI GTAC 8 cut(s) 154, 909, 937, 1322, 1872, 2085, 2696, 3267
AflII CTTAAG 1 cut(s) 2753
AflIII ACRYGT 1 cut(s) 1788
AhdI GACNNNNNGTC 1 cut(s) 2441
AjnI CCWGG 6 cut(s) 895, 1467, 1722, 1873, 2260, 3175
AjuI GAANNNNNNNTTGG 8 cut(s) 657, 689, 1612, 1644, 2444, 2476, 2777, 2809
AleI CACNNNNGTG 1 cut(s) 406
Alw21I GWGCWC 2 cut(s) 651, 3678
Alw26I GTCTC 3 cut(s) 213, 835, 2449
AlwI GGATC 8 cut(s) 31, 170, 607, 788, 1649, 2472, 2648, 3537
AlwNI CAGNNNCTG 6 cut(s) 373, 902, 1457, 1667, 1874, 3014
Ama87I CYCGRG 2 cut(s) 1881, 2968
Aor13HI TCCGGA 2 cut(s) 1033, 2323
ApaI GGGCCC 1 cut(s) 1868
ApoI RAATTY 8 cut(s) 136, 376, 1076, 2152, 2497, 2777, 3212, 3340
ArsI GACNNNNNNTTYG 2 cut(s) 325, 357
Asp700I GAANNNNTTC 2 cut(s) 1263, 3258
AspLEI GCGC 8 cut(s) 194, 227, 622, 1210, 1693, 2514, 2849, 3198
AspS9I GGNCC 9 cut(s) 1103, 1445, 1709, 1864, 1865, 2572, 2963, 3160, 3727
AsuC2I CCSGG 3 cut(s) 180, 2969, 2970
AsuHPI GGTGA 8 cut(s) 280, 445, 671, 1814, 2097, 2896, 3080, 3086
AsuII TTCGAA 2 cut(s) 140, 3313
AvaI CYCGRG 2 cut(s) 1881, 2968
AvaII GGWCC 4 cut(s) 1445, 1709, 2572, 2963
AxyI CCTNAGG 1 cut(s) 1768
BaeGI GKGCMC 1 cut(s) 1868
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BalI TGGCCA 1 cut(s) 1613
BanI GGYRCC 2 cut(s) 1678, 1690
BanII GRGCYC 3 cut(s) 1868, 1882, 3678
BbsI GAAGAC 3 cut(s) 51, 297, 2344
Bbv12I GWGCWC 2 cut(s) 651, 3678
BceAI ACGGC 4 cut(s) 180, 228, 238, 245
BcgI CGANNNNNNTGC 6 cut(s) 1330, 1364, 2447, 2481, 3410, 3444
BciT130I CCWGG 6 cut(s) 897, 1469, 1724, 1875, 2262, 3177
BciVI GTATCC 1 cut(s) 2860
BclI TGATCA 1 cut(s) 2061
BcnI CCSGG 3 cut(s) 180, 2969, 2970
BcoDI GTCTC 3 cut(s) 213, 835, 2449
BfaI CTAG 8 cut(s) 1100, 1134, 1673, 2664, 3104, 3108, 3123, 3714
BfmI CTRYAG 5 cut(s) 1404, 1449, 3006, 3537, 3543
BfoI RGCGCY 3 cut(s) 195, 1694, 2850
BfrI CTTAAG 1 cut(s) 2753
BfuI GTATCC 1 cut(s) 2860
BglI GCCNNNNNGGC 3 cut(s) 91, 167, 203
BlpI GCTNAGC 1 cut(s) 2561
Bme1390I CCNGG 9 cut(s) 180, 897, 1469, 1724, 1875, 2262, 2969, 2970, 3177
Bme18I GGWCC 4 cut(s) 1445, 1709, 2572, 2963
BmeRI GACNNNNNGTC 1 cut(s) 2441
BmeT110I CYCGRG 2 cut(s) 1881, 2968
BmgT120I GGNCC 9 cut(s) 1103, 1445, 1709, 1864, 1865, 2572, 2963, 3160, 3727
BmiI GGNNCC 7 cut(s) 689, 1680, 1692, 1865, 1866, 1879, 2965
BmrFI CCNGG 9 cut(s) 180, 897, 1469, 1724, 1875, 2262, 2969, 2970, 3177
BmrI ACTGGG 1 cut(s) 1862
BmuI ACTGGG 1 cut(s) 1862
BpiI GAAGAC 3 cut(s) 51, 297, 2344
BplI GAGNNNNNCTC 4 cut(s) 1015, 1047, 1736, 1768
BpmI CTGGAG 1 cut(s) 1896
Bpu1102I GCTNAGC 1 cut(s) 2561
Bpu14I TTCGAA 2 cut(s) 140, 3313
BpuMI CCSGG 3 cut(s) 180, 2969, 2970
Bsa29I ATCGAT 1 cut(s) 461
BsaAI YACGTR 1 cut(s) 1789
BsaHI GRCGYC 1 cut(s) 1691
BsaI GGTCTC 1 cut(s) 835
BsaWI WCCGGW 2 cut(s) 1033, 2323
Bse118I RCCGGY 4 cut(s) 157, 197, 310, 3162
Bse1I ACTGG 3 cut(s) 828, 1868, 3642
Bse21I CCTNAGG 1 cut(s) 1768
Bse3DI GCAATG 2 cut(s) 2011, 3198
BseAI TCCGGA 2 cut(s) 1033, 2323
BseBI CCWGG 6 cut(s) 897, 1469, 1724, 1875, 2262, 3177
BseCI ATCGAT 1 cut(s) 461
BseMI GCAATG 2 cut(s) 2011, 3198
BseMII CTCAG 9 cut(s) 92, 221, 669, 887, 929, 1449, 1659, 1782, 2673
BseNI ACTGG 3 cut(s) 828, 1868, 3642
BseRI GAGGAG 2 cut(s) 28, 1899
BseSI GKGCMC 1 cut(s) 1868
BseX3I CGGCCG 1 cut(s) 213
BsgI GTGCAG 4 cut(s) 237, 755, 759, 3471
Bsh1236I CGCG 2 cut(s) 227, 546
Bsh1285I CGRYCG 1 cut(s) 216
BshNI GGYRCC 2 cut(s) 1678, 1690
BshVI ATCGAT 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 651, 3678
BsiHKCI CYCGRG 2 cut(s) 1881, 2968
BslFI GGGAC 6 cut(s) 1786, 2125, 2348, 2448, 2949, 2986
BsmAI GTCTC 3 cut(s) 213, 835, 2449
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 6 cut(s) 1786, 2125, 2348, 2448, 2949, 2986
BsmI GAATGC 2 cut(s) 191, 1263
Bso31I GGTCTC 1 cut(s) 835
BsoBI CYCGRG 2 cut(s) 1881, 2968
Bsp119I TTCGAA 2 cut(s) 140, 3313
Bsp120I GGGCCC 1 cut(s) 1864
Bsp1286I GDGCHC 4 cut(s) 651, 1868, 1882, 3678
Bsp13I TCCGGA 2 cut(s) 1033, 2323
Bsp1407I TGTACA 1 cut(s) 2083
Bsp1720I GCTNAGC 1 cut(s) 2561
BspCNI CTCAG 9 cut(s) 91, 220, 668, 886, 928, 1450, 1660, 1781, 2674
BspDI ATCGAT 1 cut(s) 461
BspEI TCCGGA 2 cut(s) 1033, 2323
BspFNI CGCG 2 cut(s) 227, 546
BspHI TCATGA 2 cut(s) 2121, 3625
BspLI GGNNCC 7 cut(s) 689, 1680, 1692, 1865, 1866, 1879, 2965
BspMAI CTGCAG 2 cut(s) 1453, 3541
BspPI GGATC 8 cut(s) 31, 170, 607, 788, 1649, 2472, 2648, 3537
BspQI GCTCTTC 2 cut(s) 1705, 2310
BspT104I TTCGAA 2 cut(s) 140, 3313
BspT107I GGYRCC 2 cut(s) 1678, 1690
BspTI CTTAAG 1 cut(s) 2753
BspTNI GGTCTC 1 cut(s) 835
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 2 cut(s) 2011, 3198
BsrFI RCCGGY 4 cut(s) 157, 197, 310, 3162
BsrGI TGTACA 1 cut(s) 2083
BsrI ACTGG 3 cut(s) 828, 1868, 3642
BssAI RCCGGY 4 cut(s) 157, 197, 310, 3162
BssNAI GTATAC 1 cut(s) 1401
BssNI GRCGYC 1 cut(s) 1691
BssT1I CCWWGG 5 cut(s) 404, 1575, 1608, 1682, 2367
Bst1107I GTATAC 1 cut(s) 1401
Bst2UI CCWGG 6 cut(s) 897, 1469, 1724, 1875, 2262, 3177
Bst4CI ACNGT 6 cut(s) 44, 426, 1420, 2518, 2715, 3494
Bst6I CTCTTC 3 cut(s) 996, 1705, 2310
BstACI GRCGYC 1 cut(s) 1691
BstAFI CTTAAG 1 cut(s) 2753
BstAPI GCANNNNNTGC 1 cut(s) 642
BstAUI TGTACA 1 cut(s) 2083
BstBAI YACGTR 1 cut(s) 1789
BstBI TTCGAA 2 cut(s) 140, 3313
BstC8I GCNNGC 9 cut(s) 159, 199, 312, 413, 647, 1215, 2991, 3164, 3433
BstDSI CCRYGG 2 cut(s) 162, 261
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 2943
BstFNI CGCG 2 cut(s) 227, 546
BstH2I RGCGCY 3 cut(s) 195, 1694, 2850
BstHHI GCGC 8 cut(s) 194, 227, 622, 1210, 1693, 2514, 2849, 3198
BstMAI GTCTC 3 cut(s) 213, 835, 2449
BstMCI CGRYCG 1 cut(s) 216
BstNI CCWGG 6 cut(s) 897, 1469, 1724, 1875, 2262, 3177
BstNSI RCATGY 5 cut(s) 626, 1234, 1394, 3157, 3439
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 9 cut(s) 178, 895, 1467, 1722, 1873, 2260, 2967, 2968, 3175
BstSFI CTRYAG 5 cut(s) 1404, 1449, 3006, 3537, 3543
BstSLI GKGCMC 1 cut(s) 1868
BstUI CGCG 2 cut(s) 227, 546
BstV2I GAAGAC 3 cut(s) 51, 297, 2344
BstX2I RGATCY 2 cut(s) 23, 793
BstYI RGATCY 2 cut(s) 23, 793
BstZ17I GTATAC 1 cut(s) 1401
BstZI CGGCCG 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 461
Bsu36I CCTNAGG 1 cut(s) 1768
BsuI GTATCC 1 cut(s) 2860
BsuTUI ATCGAT 1 cut(s) 461
BtgI CCRYGG 2 cut(s) 162, 261
BtgZI GCGATG 2 cut(s) 1741, 3600
BtsI GCAGTG 2 cut(s) 602, 3586
BtsIMutI CAGTG 5 cut(s) 602, 1416, 2514, 3586, 3649
Cac8I GCNNGC 9 cut(s) 159, 199, 312, 413, 647, 1215, 2991, 3164, 3433
CaiI CAGNNNCTG 6 cut(s) 373, 902, 1457, 1667, 1874, 3014
CciI TCATGA 2 cut(s) 2121, 3625
CfoI GCGC 8 cut(s) 194, 227, 622, 1210, 1693, 2514, 2849, 3198
Cfr10I RCCGGY 4 cut(s) 157, 197, 310, 3162
Cfr13I GGNCC 9 cut(s) 1103, 1445, 1709, 1864, 1865, 2572, 2963, 3160, 3727
Cfr9I CCCGGG 1 cut(s) 2968
ClaI ATCGAT 1 cut(s) 461
Csp6I GTAC 8 cut(s) 153, 908, 936, 1321, 1871, 2084, 2695, 3266
CspCI CAANNNNNGTGG 2 cut(s) 2982, 3017
CviQI GTAC 8 cut(s) 153, 908, 936, 1321, 1871, 2084, 2695, 3266
DinI GGCGCC 1 cut(s) 1692
DraI TTTAAA 3 cut(s) 1255, 1795, 3616
DriI GACNNNNNGTC 1 cut(s) 2441
EaeI YGGCCR 3 cut(s) 159, 213, 1611
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 3 cut(s) 996, 1705, 2310
Eam1105I GACNNNNNGTC 1 cut(s) 2441
EarI CTCTTC 3 cut(s) 996, 1705, 2310
EciI GGCGGA 1 cut(s) 734
Ecl136II GAGCTC 1 cut(s) 3676
EclXI CGGCCG 1 cut(s) 213
Eco130I CCWWGG 5 cut(s) 404, 1575, 1608, 1682, 2367
Eco147I AGGCCT 1 cut(s) 3286
Eco24I GRGCYC 3 cut(s) 1868, 1882, 3678
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 4 cut(s) 1445, 1709, 2572, 2963
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 3676
Eco57I CTGAAG 7 cut(s) 264, 309, 318, 524, 783, 924, 2667
Eco81I CCTNAGG 1 cut(s) 1768
Eco88I CYCGRG 2 cut(s) 1881, 2968
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 3676
EcoNI CCTNNNNNAGG 1 cut(s) 2943
EcoO109I RGGNCCY 3 cut(s) 1103, 1864, 3727
EcoO65I GGTNACC 1 cut(s) 268
EcoRI GAATTC 2 cut(s) 136, 376
EcoRII CCWGG 6 cut(s) 895, 1467, 1722, 1873, 2260, 3175
EcoT14I CCWWGG 5 cut(s) 404, 1575, 1608, 1682, 2367
EcoT38I GRGCYC 3 cut(s) 1868, 1882, 3678
EgeI GGCGCC 1 cut(s) 1692
EheI GGCGCC 1 cut(s) 1692
ErhI CCWWGG 5 cut(s) 404, 1575, 1608, 1682, 2367
Esp3I CGTCTC 1 cut(s) 213
FalI AAGNNNNNCTT 4 cut(s) 933, 965, 2937, 2969
FaqI GGGAC 6 cut(s) 1786, 2125, 2348, 2448, 2949, 2986
FauI CCCGC 3 cut(s) 282, 546, 3159
FauNDI CATATG 2 cut(s) 1717, 2418
FbaI TGATCA 1 cut(s) 2061
FblI GTMKAC 2 cut(s) 1400, 2269
FriOI GRGCYC 3 cut(s) 1868, 1882, 3678
FspBI CTAG 8 cut(s) 1100, 1134, 1673, 2664, 3104, 3108, 3123, 3714
FspI TGCGCA 2 cut(s) 2513, 3197
GlaI GCGC 8 cut(s) 193, 226, 621, 1209, 1692, 2513, 2848, 3197
GsuI CTGGAG 1 cut(s) 1896
HaeII RGCGCY 3 cut(s) 195, 1694, 2850
HhaI GCGC 8 cut(s) 194, 227, 622, 1210, 1693, 2514, 2849, 3198
Hin1I GRCGYC 1 cut(s) 1691
Hin6I GCGC 8 cut(s) 192, 225, 620, 1208, 1691, 2512, 2847, 3196
HinP1I GCGC 8 cut(s) 192, 225, 620, 1208, 1691, 2512, 2847, 3196
HincII GTYRAC 3 cut(s) 396, 3148, 3664
HindII GTYRAC 3 cut(s) 396, 3148, 3664
HindIII AAGCTT 2 cut(s) 246, 413
HphI GGTGA 8 cut(s) 280, 445, 671, 1814, 2097, 2896, 3080, 3086
Hpy99I CGWCG 2 cut(s) 58, 68
HpyCH4III ACNGT 6 cut(s) 44, 426, 1420, 2518, 2715, 3494
HpyCH4IV ACGT 2 cut(s) 1223, 1788
HpySE526I ACGT 2 cut(s) 1223, 1788
Hsp92I GRCGYC 1 cut(s) 1691
HspAI GCGC 8 cut(s) 192, 225, 620, 1208, 1691, 2512, 2847, 3196
KasI GGCGCC 1 cut(s) 1690
Kpn2I TCCGGA 2 cut(s) 1033, 2323
KroI GCCGGC 4 cut(s) 157, 197, 310, 3162
KroNI GCCGGC 4 cut(s) 159, 199, 312, 3164
Ksp22I TGATCA 1 cut(s) 2061
LguI GCTCTTC 2 cut(s) 1705, 2310
LmnI GCTCC 4 cut(s) 1877, 2908, 3181, 3358
MaeI CTAG 8 cut(s) 1100, 1134, 1673, 2664, 3104, 3108, 3123, 3714
MaeII ACGT 2 cut(s) 1223, 1788
MbiI CCGCTC 1 cut(s) 561
MfeI CAATTG 3 cut(s) 471, 2459, 3426
MflI RGATCY 2 cut(s) 23, 793
MhlI GDGCHC 4 cut(s) 651, 1868, 1882, 3678
MlsI TGGCCA 1 cut(s) 1613
MluNI TGGCCA 1 cut(s) 1613
Mly113I GGCGCC 1 cut(s) 1691
MlyI GAGTC 4 cut(s) 1033, 1745, 3387, 3665
MmeI TCCRAC 2 cut(s) 87, 739
Mox20I TGGCCA 1 cut(s) 1613
MreI CGCCGGCG 1 cut(s) 197
MroI TCCGGA 2 cut(s) 1033, 2323
MroNI GCCGGC 4 cut(s) 157, 197, 310, 3162
MroXI GAANNNNTTC 2 cut(s) 1263, 3258
MscI TGGCCA 1 cut(s) 1613
MslI CAYNNNNRTG 6 cut(s) 406, 1235, 2262, 2417, 3038, 3058
Msp20I TGGCCA 1 cut(s) 1613
MspA1I CMGCKG 3 cut(s) 317, 553, 642
MspCI CTTAAG 1 cut(s) 2753
MspR9I CCNGG 9 cut(s) 180, 897, 1469, 1724, 1875, 2262, 2969, 2970, 3177
MunI CAATTG 3 cut(s) 471, 2459, 3426
Mva1269I GAATGC 2 cut(s) 191, 1263
MvaI CCWGG 6 cut(s) 897, 1469, 1724, 1875, 2262, 3177
MvnI CGCG 2 cut(s) 227, 546
NaeI GCCGGC 4 cut(s) 159, 199, 312, 3164
NarI GGCGCC 1 cut(s) 1691
NciI CCSGG 3 cut(s) 180, 2969, 2970
NdeI CATATG 2 cut(s) 1717, 2418
NgoMIV GCCGGC 4 cut(s) 157, 197, 310, 3162
NlaIV GGNNCC 7 cut(s) 689, 1680, 1692, 1865, 1866, 1879, 2965
NmeAIII GCCGAG 1 cut(s) 1591
NmuCI GTSAC 6 cut(s) 268, 420, 1802, 2748, 2764, 3458
NsbI TGCGCA 2 cut(s) 2513, 3197
NspI RCATGY 5 cut(s) 626, 1234, 1394, 3157, 3439
NspV TTCGAA 2 cut(s) 140, 3313
OliI CACNNNNGTG 1 cut(s) 406
PagI TCATGA 2 cut(s) 2121, 3625
PceI AGGCCT 1 cut(s) 3286
PciSI GCTCTTC 2 cut(s) 1705, 2310
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 2 cut(s) 191, 1263
PdiI GCCGGC 4 cut(s) 159, 199, 312, 3164
PdmI GAANNNNTTC 2 cut(s) 1263, 3258
PfeI GAWTC 9 cut(s) 436, 1014, 1049, 1423, 1727, 1995, 2159, 2641, 2706
PflMI CCANNNNNTGG 1 cut(s) 1874
PfoI TCCNGGA 1 cut(s) 178
PleI GAGTC 4 cut(s) 1032, 1745, 3386, 3664
PluTI GGCGCC 1 cut(s) 1694
PpsI GAGTC 4 cut(s) 1032, 1745, 3386, 3664
Ppu21I YACGTR 1 cut(s) 1789
PsiI TTATAA 1 cut(s) 1140
Psp124BI GAGCTC 1 cut(s) 3678
Psp6I CCWGG 6 cut(s) 895, 1467, 1722, 1873, 2260, 3175
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 6 cut(s) 895, 1467, 1722, 1873, 2260, 3175
PspN4I GGNNCC 7 cut(s) 689, 1680, 1692, 1865, 1866, 1879, 2965
PspOMI GGGCCC 1 cut(s) 1864
PspPI GGNCC 9 cut(s) 1103, 1445, 1709, 1864, 1865, 2572, 2963, 3160, 3727
PstI CTGCAG 2 cut(s) 1453, 3541
PstNI CAGNNNCTG 6 cut(s) 373, 902, 1457, 1667, 1874, 3014
PsuI RGATCY 2 cut(s) 23, 793
PvuII CAGCTG 2 cut(s) 317, 642
RsaI GTAC 8 cut(s) 154, 909, 937, 1322, 1872, 2085, 2696, 3267
RsaNI GTAC 8 cut(s) 153, 908, 936, 1321, 1871, 2084, 2695, 3266
RseI CAYNNNNRTG 6 cut(s) 406, 1235, 2262, 2417, 3038, 3058
SacI GAGCTC 1 cut(s) 3678
SapI GCTCTTC 2 cut(s) 1705, 2310
Sau96I GGNCC 9 cut(s) 1103, 1445, 1709, 1864, 1865, 2572, 2963, 3160, 3727
SbfI CCTGCAGG 1 cut(s) 1453
SchI GAGTC 4 cut(s) 1033, 1745, 3387, 3665
ScrFI CCNGG 9 cut(s) 180, 897, 1469, 1724, 1875, 2262, 2969, 2970, 3177
SdaI CCTGCAGG 1 cut(s) 1453
SduI GDGCHC 4 cut(s) 651, 1868, 1882, 3678
SfcI CTRYAG 5 cut(s) 1404, 1449, 3006, 3537, 3543
SfoI GGCGCC 1 cut(s) 1692
SfuI TTCGAA 2 cut(s) 140, 3313
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 4 cut(s) 1445, 1709, 2572, 2963
SmaI CCCGGG 1 cut(s) 2970
SmiMI CAYNNNNRTG 6 cut(s) 406, 1235, 2262, 2417, 3038, 3058
SmlI CTYRAG 1 cut(s) 2753
SmoI CTYRAG 1 cut(s) 2753
Sse8387I CCTGCAGG 1 cut(s) 1453
SseBI AGGCCT 1 cut(s) 3286
SspDI GGCGCC 1 cut(s) 1690
SspI AATATT 2 cut(s) 1434, 1499
SspMI CTAG 8 cut(s) 1100, 1134, 1673, 2664, 3104, 3108, 3123, 3714
SstI GAGCTC 1 cut(s) 3678
StuI AGGCCT 1 cut(s) 3286
StyD4I CCNGG 9 cut(s) 178, 895, 1467, 1722, 1873, 2260, 2967, 2968, 3175
StyI CCWWGG 5 cut(s) 404, 1575, 1608, 1682, 2367
TaaI ACNGT 6 cut(s) 44, 426, 1420, 2518, 2715, 3494
TaiI ACGT 2 cut(s) 1226, 1791
TatI WGTACW 1 cut(s) 2083
TauI GCSGC 6 cut(s) 94, 170, 173, 197, 280, 872
TfiI GAWTC 9 cut(s) 436, 1014, 1049, 1423, 1727, 1995, 2159, 2641, 2706
TscAI CASTG 5 cut(s) 609, 1423, 2521, 3586, 3649
TseFI GTSAC 6 cut(s) 268, 420, 1802, 2748, 2764, 3458
Tsp45I GTSAC 6 cut(s) 268, 420, 1802, 2748, 2764, 3458
TspGWI ACGGA 4 cut(s) 146, 1757, 2712, 2783
TspMI CCCGGG 1 cut(s) 2968
TspRI CASTG 5 cut(s) 609, 1423, 2521, 3586, 3649
Van91I CCANNNNNTGG 1 cut(s) 1874
Vha464I CTTAAG 1 cut(s) 2753
VpaK11BI GGWCC 4 cut(s) 1445, 1709, 2572, 2963
XagI CCTNNNNNAGG 1 cut(s) 2943
XapI RAATTY 8 cut(s) 136, 376, 1076, 2152, 2497, 2777, 3212, 3340
XbaI TCTAGA 1 cut(s) 3713
XceI RCATGY 5 cut(s) 626, 1234, 1394, 3157, 3439
XmaI CCCGGG 1 cut(s) 2968
XmiI GTMKAC 2 cut(s) 1400, 2269
XmnI GAANNNNTTC 2 cut(s) 1263, 3258
XspI CTAG 8 cut(s) 1100, 1134, 1673, 2664, 3104, 3108, 3123, 3714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.