MD06G1143300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004286
Physical Location & Seq
Forward (+)
28688556 .. 28699598
11043 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1143300.v1.1.491

Sequence Viewer

Length: 3597 bp
ATGGTTTCAACGAGGCGAAGTGGATCTCTCTCCGGGAACAACTGCAAACGATCGTCGTCTTCCGAGGACAAGCCACCGTCACCGAAACGACCGAAGGCGGATAATGGTAGTGCTTCGGACAAAGCGACTCCTGGGGTGGAGAATTCGAAGGAGCTGTGCACGCTTCCGCCGGCGGGGGCTGCCGATCCCGGAGAATGTGGTCCCGAAGATGCGCCGGTCGCCGGAGACGGGGTGACTTCTGGAAAGACCGAAGCTGCTGCGCCAGCTGTGGCGGTGACGACACCGATCGCCGAAGGGTCTTCGCCGGTGGTGGAAAAGCAGCCGAGGAGTGCACCGTCTTCCTGGAGTTTCTATCAGAAGCAGAACCCGAGTTTTGATACGCCTTGGTGCAAGCTTTTGTCACAGTCTGCACTGAATGTAAATATTTCTATTAGTGCAATGGCATTCACAATTGGTGCAAACAGACAGTGCAATTTTGCTCTCAAGGATCATACCATTAGTGGAGTACTGTGCAAGATCAAGCGCACACAGCGTGAGGGCAGTGCTGTAGCAGTTCTTGAAAGTACGGGAAGCAAGGGATCAGTGCAAGTAAATGGGACAAATGTCAAGAAAGGCAACAGTTGCATGCTTAACCCGGGTGATGAAGTTGTCTTTGGTTCAATGGGCAACCATGCTTATATTTTTCAGCTACTGCTGACTGAGGCTGCAGTTAAGGGTGCAGAGGTGCAAGGCAGTATTGGAAAATTTTTGCATCTTGAAAAGAGGGCAGGAGATCCTTCAGCTGTGGCTGGGGCTTCCATACTGGCTTCTCTTAGCACGAGGGCAGAACAATCGCGATGGAAGTCCGCAGCTCAGACCACCAGTAAAGTTCACCCCGGTGCTGAGGTACCAGCTCAGTCTGTCATTCAAGATGATACAGAAATTGAGCTTGGTGGTCTAGAAAGCAGCTCAACTCCAAATAGAGCGACTGACAAAGCTGAAGATATTGGAGCAATAGACAAGAATCTCACTCCAGACTGCAACCCCGATTCTAGCATAGAGGTATTGGAAGAAAGAAACGAGTGGATGAGGGATTTGCAATCAGCATCAACGTCAGGCATGTCTCTCCGGTGTGTTGCATTTAAAGAAGGCCTTCATGCAGGAATTCTTGATGGCAAAAGCATAGATGTCTCCTTTGATAACTTCCCATACTATTTGAGTGAGAATACGAAGAAAGTGTTGATTGCAGCCTCATTTATACACTTGAAACATAAAGAGCATGTGAAGTATACCTCAGAGCTTACAGCTGTGAACCCTCGCATTTTGCTCTCTGGTCCTTCAGGGTCTGAGATATATCAGGAGATGTTGGCAAAGGCTCTTGCGCAGTACTTTGGGGCTAAGTTGCTCTTATTCGATAGCCACTCTTTTTTGGGTGGTTTATCATCAAAGGAAGCTGAGCTGCTCAGGGATGGATTGAGTGCAGAAAAACTCTGCAGCTTGACCAAACAAAGTCCCGCACTCCCAGACTTGGCCAAGAACACTGATCTTTCAGCTTCTGAAACAGAGGCACTGGGATCTTCAAATGCACTGAATGACCTGGAATCCCAACCAAAGATGGAGAATGACACCCTCCCCTCTTCCTCGGGGGCATCGAGGAATTACTTGTTTAAAATAGGTGACAGAGTAAGATTTATTGCTCCAGGTGCCTTATATGCTACATCATCTTCTTTACGGGGCCCAGGGATCGGAATGCGTGGAGAGGTTGTGCTGCTTTTTAAGGACAATCCTTTGTCAAAAGTCGGTGTAAAGTTTGATAAACCAATACCTGATGGTGTTGATCTTGGGGGTCTATGTAAAGGCAATGGATACTTCTGCAATGTCTTTGATCTTCGATTGGAAACCACGGGCGCAGAAGATTTGGACAAGTTACTTATCAACACATTGTTTGAGGCTGTAATAAGTGAGAGCAGAAGTTCCCCTTTCATTTTGTTCATGAAAGATGCTGAGAAGTCCCTGGTAGGAAATTCTGATTCATTTTCCGCATTCAGAGCGAGACTTGATAAGCTTCCAGATAATGTGGTTGTAATTGGTTCTCACACTCAAACTGACAGTCGCAAGGAGAAGTCGCACCCTGGTGGTTTGCTTTTCACAAAGTTTGGCAGCAATCAAACTGCTCTTCTTGACTTGGCTTTCCCGGATAGTTTTGGAAGACTACATGAGAGAGGGAAAGAAGTTCCCAAGGCGACAAAACTTCTGTCTAAACTCTTTCCCAATAAAGTTACCATTCATATGCCGCAGGATGAAGCACTTCTTGTCTCGTGGAAGCAACAATTGGATCGAGATGTTGAAACCCTAAAAATGAAGGGAAACTTGAATCTCTTGCGCACTGTTCTGGGTCGATGTGGACTAGAGTGTGAAGGACTTGAGACACTATGCATCAAGGATCAAACACTTACCAACGAAAGTTCAGACAAGGTAGTTGGATGGGCTCTAAACCATCATTTAATGCAGAATCCTGAAGCTGATCCAGAAACAAAAGTTGTTGTATCTGCGGAGAGCATCCAGTACGGACTTGAAATTTTACAGGCTCTCCAGAATGAAACGAAGAGTTTGAAGAAGTCACTTAAGGATGTTGTAACAGAAAATGAATTTGAGAAAAGGCTGTTAGCTGATGTTATTCCACCTAGTGACATTGGAGTTACGTTTGATGATATTGGAGCCCTTGAGAATGTGAAGGATACATTGAAGGAGTTGGTGATGCTTCCTTTACAAAGGCCTGAGCTTTTCTGCAAGGGGCAATTAACCAAGCCTTGCAAGGGTATCCTTTTATTTGGTCCCCCTGGAACAGGCAAGACAATGCTTGCAAAGGCTGTGGCCACGGAAGCTGGTGCAAACTTTATCAACATATCCATGTCAAGCATCACATCTAAGTGGTTTGGTGAGGGTGAGAAATACGTGAAAGCTGTTTTCTCGCTGGCCAGTAAAATTGCGCCTAGTGTTGTATTCGTAGATGAAGTGGACAGTATGTTGGGCCGACGGGAAAATCCAGGGGAGCATGAGGCAATGCGTAAGATGAAGAATGAATTTATGGTAAATTGGGATGGTTTACGAACAAAAGAAACAGAGCGAGTTCTTGTACTGGCAGCCACAAATAGGCCTTTTGACCTTGATGAGGCTGTCATTCGAAGACTGCCGCGCAGGTTGATGGTAAATTTGCCAGATGCTCCGAATAGAGCAAAAATATTGAAAGTCATACTGGCAAAAGAGGACTTGTCTCCCACTATTGATTTTGATGCTATTGCGAGCATGACGGATGGGTATTCTGGAAGTGACCTCAAGAATCTTTGTGTAACTGCTGCACATCGTCCAATTAAAGAGATTTTGGAGAAGGAAAAAAAGGAGCATGCTGTAGCTGTAGCAGAAGGTAGAGCTGCTCCAGCTTTGAGTGGCAGTGCTGATATACGGTCTCTGAACATGGACGACTTCAAAGATGCTCACGAACGGGTATGTGCCAGTGTTTCATCGGAGTCTGTGAACATGACCGAGCTTTTACAGTGGAACGAATTATACGGCGAAGGGGGTTCAAGAAGAAAGAAAGCCCTTAGCTACTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

1199

Amino Acids

129.51

Weight (kDa)

6.01

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 150 - 219 1.2e-06 FHA domain
DUF7751 PF24933 796 - 855 3e-19 Domain of unknown function (DUF7751)
AAA PF00004 934 - 1064 1.2e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 1088 - 1127 1.4e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1362, 2363
Acc36I ACCTGC 1 cut(s) 3168
Acc65I GGTACC 1 cut(s) 886
AccB1I GGYRCC 2 cut(s) 886, 1682
AccI GTMKAC 1 cut(s) 1268
AccII CGCG 2 cut(s) 835, 3175
AcoI YGGCCR 3 cut(s) 1509, 2853, 2955
AcsI RAATTY 8 cut(s) 142, 743, 1143, 2002, 2556, 2627, 3062, 3190
AcuI CTGAAG 4 cut(s) 762, 999, 1302, 2517
AdeI CACNNNGTG 2 cut(s) 533, 2666
AfaI GTAC 6 cut(s) 507, 565, 888, 1367, 2546, 3117
AfiI CCNNNNNNNGG 9 cut(s) 173, 221, 228, 1507, 1724, 2795, 2825, 3132, 3151
AflII CTTAAG 1 cut(s) 2603
AjnI CCWGG 9 cut(s) 130, 341, 1575, 1678, 1717, 1992, 2110, 2818, 3025
AjuI GAANNNNNNNTTGG 6 cut(s) 636, 668, 912, 944, 2294, 2326
AleI CACNNNNGTG 2 cut(s) 876, 2112
Alw21I GWGCWC 2 cut(s) 161, 334
Alw26I GTCTC 8 cut(s) 219, 1107, 1174, 2026, 2299, 2399, 3258, 3450
Alw44I GTGCAC 2 cut(s) 157, 330
AlwNI CAGNNNCTG 3 cut(s) 691, 1325, 1535
Ama87I CYCGRG 3 cut(s) 367, 634, 1621
AoxI GGCC 8 cut(s) 1129, 1509, 1714, 2753, 2853, 2955, 3010, 3134
ApaI GGGCCC 1 cut(s) 1718
ApaLI GTGCAC 2 cut(s) 157, 330
ApoI RAATTY 8 cut(s) 142, 743, 1143, 2002, 2556, 2627, 3062, 3190
ArsI GACNNNNNNTTYG 4 cut(s) 2074, 2106, 2219, 2251
Asp700I GAANNNNTTC 2 cut(s) 1131, 2286
Asp718I GGTACC 1 cut(s) 886
AspLEI GCGC 8 cut(s) 214, 262, 525, 1363, 1889, 2364, 2971, 3177
AspS9I GGNCC 6 cut(s) 200, 1313, 1714, 1715, 2813, 3010
AsuC2I CCSGG 6 cut(s) 34, 189, 635, 636, 876, 2174
AsuHPI GGTGA 9 cut(s) 72, 244, 286, 650, 863, 1667, 2746, 2930, 2936
AsuII TTCGAA 2 cut(s) 146, 3163
AvaI CYCGRG 3 cut(s) 367, 634, 1621
AvaII GGWCC 3 cut(s) 200, 1313, 2813
BaeGI GKGCMC 3 cut(s) 161, 334, 1718
BalI TGGCCA 3 cut(s) 1511, 2855, 2957
BanI GGYRCC 2 cut(s) 886, 1682
BanII GRGCYC 3 cut(s) 1718, 2470, 2701
BauI CACGAG 2 cut(s) 817, 2296
BbsI GAAGAC 5 cut(s) 51, 291, 330, 2194, 3172
Bbv12I GWGCWC 2 cut(s) 161, 334
BbvCI CCTCAGC 1 cut(s) 882
BceAI ACGGC 1 cut(s) 3565
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 813, 847
BciT130I CCWGG 9 cut(s) 132, 343, 1577, 1680, 1719, 1994, 2112, 2820, 3027
BciVI GTATCC 3 cut(s) 1838, 2710, 2810
BcnI CCSGG 6 cut(s) 34, 189, 635, 636, 876, 2174
BcoDI GTCTC 8 cut(s) 219, 1107, 1174, 2026, 2299, 2399, 3258, 3450
BfaI CTAG 5 cut(s) 938, 1032, 2387, 2664, 2973
BfmI CTRYAG 5 cut(s) 546, 705, 1471, 3387, 3393
BfrI CTTAAG 1 cut(s) 2603
BfuAI ACCTGC 1 cut(s) 3168
BfuI GTATCC 3 cut(s) 1838, 2710, 2810
BlpI GCTNAGC 1 cut(s) 1434
BmcAI AGTACT 2 cut(s) 507, 1367
Bme18I GGWCC 3 cut(s) 200, 1313, 2813
BmeT110I CYCGRG 3 cut(s) 367, 634, 1621
BmgT120I GGNCC 6 cut(s) 200, 1313, 1714, 1715, 2813, 3010
BmiI GGNNCC 7 cut(s) 202, 888, 1684, 1715, 1716, 2698, 2815
BmrI ACTGGG 1 cut(s) 1559
BmuI ACTGGG 1 cut(s) 1559
BoxI GACNNNNGTC 1 cut(s) 602
BpiI GAAGAC 5 cut(s) 51, 291, 330, 2194, 3172
BpmI CTGGAG 5 cut(s) 364, 996, 1662, 2555, 3399
Bpu10I CCTNAGC 4 cut(s) 882, 1442, 2757, 3581
Bpu1102I GCTNAGC 1 cut(s) 1434
Bpu14I TTCGAA 2 cut(s) 146, 3163
BpuEI CTTGAG 4 cut(s) 467, 2423, 2723, 3299
BpuMI CCSGG 6 cut(s) 34, 189, 635, 636, 876, 2174
BsaAI YACGTR 1 cut(s) 2935
BsaI GGTCTC 1 cut(s) 3450
BsaWI WCCGGW 1 cut(s) 1107
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 1728, 1758, 2553, 2583
Bsc4I CCNNNNNNNGG 9 cut(s) 173, 221, 228, 1507, 1724, 2795, 2825, 3132, 3151
Bse118I RCCGGY 3 cut(s) 169, 214, 304
Bse1I ACTGG 8 cut(s) 807, 861, 1554, 2542, 2958, 3123, 3240, 3492
Bse3DI GCAATG 4 cut(s) 444, 1846, 1861, 3048
BseBI CCWGG 9 cut(s) 132, 343, 1577, 1680, 1719, 1994, 2112, 2820, 3027
BseGI GGATG 8 cut(s) 1071, 1453, 2284, 2468, 2538, 2614, 3085, 3298
BseLI CCNNNNNNNGG 9 cut(s) 173, 221, 228, 1507, 1724, 2795, 2825, 3132, 3151
BseMI GCAATG 4 cut(s) 444, 1846, 1861, 3048
BseNI ACTGG 8 cut(s) 807, 861, 1554, 2542, 2958, 3123, 3240, 3492
BseRI GAGGAG 1 cut(s) 340
BseSI GKGCMC 3 cut(s) 161, 334, 1718
BseYI CCCAGC 1 cut(s) 788
BsgI GTGCAG 4 cut(s) 393, 738, 1479, 3321
Bsh1236I CGCG 2 cut(s) 835, 3175
Bsh1285I CGRYCG 4 cut(s) 53, 92, 219, 288
BshFI GGCC 8 cut(s) 1131, 1511, 1716, 2755, 2855, 2957, 3012, 3136
BshNI GGYRCC 2 cut(s) 886, 1682
BsiEI CGRYCG 4 cut(s) 53, 92, 219, 288
BsiHKAI GWGCWC 2 cut(s) 161, 334
BsiHKCI CYCGRG 3 cut(s) 367, 634, 1621
BslFI GGGAC 5 cut(s) 186, 610, 1476, 1975, 2799
BslI CCNNNNNNNGG 9 cut(s) 173, 221, 228, 1507, 1724, 2795, 2825, 3132, 3151
BsmAI GTCTC 8 cut(s) 219, 1107, 1174, 2026, 2299, 2399, 3258, 3450
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 5 cut(s) 186, 610, 1476, 1975, 2799
BsmI GAATGC 3 cut(s) 443, 1734, 2021
BsnI GGCC 8 cut(s) 1131, 1511, 1716, 2755, 2855, 2957, 3012, 3136
Bso31I GGTCTC 1 cut(s) 3450
BsoBI CYCGRG 3 cut(s) 367, 634, 1621
Bsp119I TTCGAA 2 cut(s) 146, 3163
Bsp120I GGGCCC 1 cut(s) 1714
Bsp1286I GDGCHC 5 cut(s) 161, 334, 1718, 2470, 2701
Bsp1720I GCTNAGC 1 cut(s) 1434
Bsp68I TCGCGA 1 cut(s) 835
BspANI GGCC 8 cut(s) 1131, 1511, 1716, 2755, 2855, 2957, 3012, 3136
BspFNI CGCG 2 cut(s) 835, 3175
BspHI TCATGA 1 cut(s) 1971
BspLI GGNNCC 7 cut(s) 202, 888, 1684, 1715, 1716, 2698, 2815
BspMAI CTGCAG 2 cut(s) 709, 1475
BspMI ACCTGC 1 cut(s) 3168
BspQI GCTCTTC 1 cut(s) 2160
BspT104I TTCGAA 2 cut(s) 146, 3163
BspT107I GGYRCC 2 cut(s) 886, 1682
BspTI CTTAAG 1 cut(s) 2603
BspTNI GGTCTC 1 cut(s) 3450
BsrDI GCAATG 4 cut(s) 444, 1846, 1861, 3048
BsrFI RCCGGY 3 cut(s) 169, 214, 304
BsrI ACTGG 8 cut(s) 807, 861, 1554, 2542, 2958, 3123, 3240, 3492
BssAI RCCGGY 3 cut(s) 169, 214, 304
BssNAI GTATAC 1 cut(s) 1269
BssSI CACGAG 2 cut(s) 817, 2296
BssT1I CCWWGG 2 cut(s) 383, 2217
Bst1107I GTATAC 1 cut(s) 1269
Bst2BI CACGAG 2 cut(s) 817, 2296
Bst2UI CCWGG 9 cut(s) 132, 343, 1577, 1680, 1719, 1994, 2112, 2820, 3027
Bst6I CTCTTC 3 cut(s) 1621, 2160, 2579
BstAFI CTTAAG 1 cut(s) 2603
BstAPI GCANNNNNTGC 1 cut(s) 621
BstBAI YACGTR 1 cut(s) 2935
BstBI TTCGAA 2 cut(s) 146, 3163
BstC8I GCNNGC 9 cut(s) 161, 171, 264, 392, 626, 2841, 2955, 3283, 3384
BstDSI CCRYGG 2 cut(s) 1881, 2856
BstENI CCTNNNNNAGG 2 cut(s) 2823, 3149
BstF5I GGATG 8 cut(s) 1071, 1453, 2284, 2468, 2538, 2614, 3085, 3298
BstFNI CGCG 2 cut(s) 835, 3175
BstHHI GCGC 8 cut(s) 214, 262, 525, 1363, 1889, 2364, 2971, 3177
BstMAI GTCTC 8 cut(s) 219, 1107, 1174, 2026, 2299, 2399, 3258, 3450
BstMCI CGRYCG 4 cut(s) 53, 92, 219, 288
BstNI CCWGG 9 cut(s) 132, 343, 1577, 1680, 1719, 1994, 2112, 2820, 3027
BstNSI RCATGY 4 cut(s) 628, 1102, 1262, 3386
BstPAI GACNNNNGTC 1 cut(s) 602
BstSFI CTRYAG 5 cut(s) 546, 705, 1471, 3387, 3393
BstSLI GKGCMC 3 cut(s) 161, 334, 1718
BstUI CGCG 2 cut(s) 835, 3175
BstV2I GAAGAC 5 cut(s) 51, 291, 330, 2194, 3172
BstX2I RGATCY 3 cut(s) 23, 772, 1553
BstYI RGATCY 3 cut(s) 23, 772, 1553
BstZ17I GTATAC 1 cut(s) 1269
BsuI GTATCC 3 cut(s) 1838, 2710, 2810
BsuRI GGCC 8 cut(s) 1131, 1511, 1716, 2755, 2855, 2957, 3012, 3136
BtgI CCRYGG 2 cut(s) 1881, 2856
BtgZI GCGATG 1 cut(s) 850
BtsCI GGATG 8 cut(s) 1071, 1453, 2284, 2468, 2538, 2614, 3085, 3298
BtsI GCAGTG 2 cut(s) 547, 3436
BtuMI TCGCGA 1 cut(s) 835
BveI ACCTGC 1 cut(s) 3168
Cac8I GCNNGC 9 cut(s) 161, 171, 264, 392, 626, 2841, 2955, 3283, 3384
CaiI CAGNNNCTG 3 cut(s) 691, 1325, 1535
CciI TCATGA 1 cut(s) 1971
CfoI GCGC 8 cut(s) 214, 262, 525, 1363, 1889, 2364, 2971, 3177
Cfr10I RCCGGY 3 cut(s) 169, 214, 304
Cfr13I GGNCC 6 cut(s) 200, 1313, 1714, 1715, 2813, 3010
Cfr9I CCCGGG 1 cut(s) 634
Csp6I GTAC 6 cut(s) 506, 564, 887, 1366, 2545, 3116
CspCI CAANNNNNGTGG 2 cut(s) 2832, 2867
CviQI GTAC 6 cut(s) 506, 564, 887, 1366, 2545, 3116
DraI TTTAAA 2 cut(s) 1123, 1648
DraIII CACNNNGTG 2 cut(s) 533, 2666
EaeI YGGCCR 3 cut(s) 1509, 2853, 2955
Eam1104I CTCTTC 3 cut(s) 1621, 2160, 2579
EarI CTCTTC 3 cut(s) 1621, 2160, 2579
EciI GGCGGA 2 cut(s) 113, 156
Eco130I CCWWGG 2 cut(s) 383, 2217
Eco147I AGGCCT 3 cut(s) 1131, 2755, 3136
Eco24I GRGCYC 3 cut(s) 1718, 2470, 2701
Eco31I GGTCTC 1 cut(s) 3450
Eco47I GGWCC 3 cut(s) 200, 1313, 2813
Eco57I CTGAAG 4 cut(s) 762, 999, 1302, 2517
Eco88I CYCGRG 3 cut(s) 367, 634, 1621
EcoNI CCTNNNNNAGG 2 cut(s) 2823, 3149
EcoO109I RGGNCCY 1 cut(s) 1714
EcoRI GAATTC 2 cut(s) 142, 1143
EcoRII CCWGG 9 cut(s) 130, 341, 1575, 1678, 1717, 1992, 2110, 2818, 3025
EcoT14I CCWWGG 2 cut(s) 383, 2217
EcoT22I ATGCAT 1 cut(s) 2417
EcoT38I GRGCYC 3 cut(s) 1718, 2470, 2701
ErhI CCWWGG 2 cut(s) 383, 2217
Esp3I CGTCTC 1 cut(s) 219
FaqI GGGAC 5 cut(s) 186, 610, 1476, 1975, 2799
FauI CCCGC 2 cut(s) 166, 1501
FauNDI CATATG 1 cut(s) 2268
FblI GTMKAC 1 cut(s) 1268
FokI GGATG 8 cut(s) 1078, 1460, 2291, 2475, 2525, 2621, 3092, 3305
FriOI GRGCYC 3 cut(s) 1718, 2470, 2701
FspBI CTAG 5 cut(s) 938, 1032, 2387, 2664, 2973
FspI TGCGCA 2 cut(s) 1362, 2363
GlaI GCGC 8 cut(s) 213, 261, 524, 1362, 1888, 2363, 2970, 3176
GsaI CCCAGC 1 cut(s) 792
GsuI CTGGAG 5 cut(s) 364, 996, 1662, 2555, 3399
HaeIII GGCC 8 cut(s) 1131, 1511, 1716, 2755, 2855, 2957, 3012, 3136
HhaI GCGC 8 cut(s) 214, 262, 525, 1363, 1889, 2364, 2971, 3177
Hin6I GCGC 8 cut(s) 212, 260, 523, 1361, 1887, 2362, 2969, 3175
HinP1I GCGC 8 cut(s) 212, 260, 523, 1361, 1887, 2362, 2969, 3175
HindIII AAGCTT 2 cut(s) 392, 2042
HinfI GANTC 9 cut(s) 127, 1003, 1028, 1580, 2009, 2353, 2491, 3319, 3506
HphI GGTGA 9 cut(s) 72, 244, 286, 650, 863, 1667, 2746, 2930, 2936
Hpy166II GTNNAC 9 cut(s) 159, 332, 871, 1269, 1291, 2384, 2998, 3086, 3514
Hpy8I GTNNAC 9 cut(s) 159, 332, 871, 1269, 1291, 2384, 2998, 3086, 3514
Hpy99I CGWCG 2 cut(s) 58, 3018
HpyCH4IV ACGT 3 cut(s) 1091, 2681, 2934
HpySE526I ACGT 3 cut(s) 1091, 2681, 2934
HspAI GCGC 8 cut(s) 212, 260, 523, 1361, 1887, 2362, 2969, 3175
KpnI GGTACC 1 cut(s) 890
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
LguI GCTCTTC 1 cut(s) 2160
LmnI GCTCC 8 cut(s) 151, 989, 1681, 2696, 3031, 3208, 3379, 3418
MaeI CTAG 5 cut(s) 938, 1032, 2387, 2664, 2973
MaeII ACGT 3 cut(s) 1091, 2681, 2934
MfeI CAATTG 2 cut(s) 450, 2309
MflI RGATCY 3 cut(s) 23, 772, 1553
MhlI GDGCHC 5 cut(s) 161, 334, 1718, 2470, 2701
MlsI TGGCCA 3 cut(s) 1511, 2855, 2957
MluNI TGGCCA 3 cut(s) 1511, 2855, 2957
MlyI GAGTC 2 cut(s) 121, 3515
MmeI TCCRAC 1 cut(s) 2440
Mox20I TGGCCA 3 cut(s) 1511, 2855, 2957
Mph1103I ATGCAT 1 cut(s) 2417
MreI CGCCGGCG 1 cut(s) 169
MroNI GCCGGC 1 cut(s) 169
MroXI GAANNNNTTC 2 cut(s) 1131, 2286
MscI TGGCCA 3 cut(s) 1511, 2855, 2957
MseI TTAA 9 cut(s) 630, 711, 1122, 1647, 1755, 2483, 2604, 2780, 3351
MslI CAYNNNNRTG 5 cut(s) 876, 2112, 2267, 2888, 2908
Msp20I TGGCCA 3 cut(s) 1511, 2855, 2957
MspA1I CMGCKG 3 cut(s) 266, 782, 1286
MspCI CTTAAG 1 cut(s) 2603
MunI CAATTG 2 cut(s) 450, 2309
Mva1269I GAATGC 3 cut(s) 443, 1734, 2021
MvaI CCWGG 9 cut(s) 132, 343, 1577, 1680, 1719, 1994, 2112, 2820, 3027
MvnI CGCG 2 cut(s) 835, 3175
NaeI GCCGGC 1 cut(s) 171
NciI CCSGG 6 cut(s) 34, 189, 635, 636, 876, 2174
NdeI CATATG 1 cut(s) 2268
NgoMIV GCCGGC 1 cut(s) 169
NlaIV GGNNCC 7 cut(s) 202, 888, 1684, 1715, 1716, 2698, 2815
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 8 cut(s) 78, 232, 274, 399, 1655, 2598, 2666, 3308
NruI TCGCGA 1 cut(s) 835
NsbI TGCGCA 2 cut(s) 1362, 2363
NsiI ATGCAT 1 cut(s) 2417
NspI RCATGY 4 cut(s) 628, 1102, 1262, 3386
NspV TTCGAA 2 cut(s) 146, 3163
OliI CACNNNNGTG 2 cut(s) 876, 2112
PaeI GCATGC 2 cut(s) 628, 3386
PagI TCATGA 1 cut(s) 1971
PasI CCCWGGG 1 cut(s) 1718
PceI AGGCCT 3 cut(s) 1131, 2755, 3136
PciSI GCTCTTC 1 cut(s) 2160
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 1628
PctI GAATGC 3 cut(s) 443, 1734, 2021
PdiI GCCGGC 1 cut(s) 171
PdmI GAANNNNTTC 2 cut(s) 1131, 2286
PfeI GAWTC 7 cut(s) 1003, 1028, 1580, 2009, 2353, 2491, 3319
PfoI TCCNGGA 4 cut(s) 32, 187, 341, 2172
Ple19I CGATCG 2 cut(s) 53, 288
PleI GAGTC 2 cut(s) 121, 3514
PpsI GAGTC 2 cut(s) 121, 3514
Ppu21I YACGTR 1 cut(s) 2935
PshAI GACNNNNGTC 1 cut(s) 602
Psp6I CCWGG 9 cut(s) 130, 341, 1575, 1678, 1717, 1992, 2110, 2818, 3025
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 9 cut(s) 130, 341, 1575, 1678, 1717, 1992, 2110, 2818, 3025
PspN4I GGNNCC 7 cut(s) 202, 888, 1684, 1715, 1716, 2698, 2815
PspOMI GGGCCC 1 cut(s) 1714
PspPI GGNCC 6 cut(s) 200, 1313, 1714, 1715, 2813, 3010
PsrI GAACNNNNNNTAC 2 cut(s) 3530, 3562
PstI CTGCAG 2 cut(s) 709, 1475
PstNI CAGNNNCTG 3 cut(s) 691, 1325, 1535
PsuI RGATCY 3 cut(s) 23, 772, 1553
PvuI CGATCG 2 cut(s) 53, 288
PvuII CAGCTG 3 cut(s) 266, 782, 1286
RruI TCGCGA 1 cut(s) 835
RsaI GTAC 6 cut(s) 507, 565, 888, 1367, 2546, 3117
RsaNI GTAC 6 cut(s) 506, 564, 887, 1366, 2545, 3116
RseI CAYNNNNRTG 5 cut(s) 876, 2112, 2267, 2888, 2908
SapI GCTCTTC 1 cut(s) 2160
SaqAI TTAA 9 cut(s) 630, 711, 1122, 1647, 1755, 2483, 2604, 2780, 3351
Sau96I GGNCC 6 cut(s) 200, 1313, 1714, 1715, 2813, 3010
ScaI AGTACT 2 cut(s) 507, 1367
SchI GAGTC 2 cut(s) 121, 3515
SduI GDGCHC 5 cut(s) 161, 334, 1718, 2470, 2701
SfcI CTRYAG 5 cut(s) 546, 705, 1471, 3387, 3393
SfuI TTCGAA 2 cut(s) 146, 3163
SgrAI CRCCGGYG 2 cut(s) 169, 304
SinI GGWCC 3 cut(s) 200, 1313, 2813
SmaI CCCGGG 1 cut(s) 636
SmiMI CAYNNNNRTG 5 cut(s) 876, 2112, 2267, 2888, 2908
SmlI CTYRAG 5 cut(s) 482, 2402, 2603, 2702, 3314
SmoI CTYRAG 5 cut(s) 482, 2402, 2603, 2702, 3314
SphI GCATGC 2 cut(s) 628, 3386
SseBI AGGCCT 3 cut(s) 1131, 2755, 3136
SspI AATATT 2 cut(s) 424, 3222
SspMI CTAG 5 cut(s) 938, 1032, 2387, 2664, 2973
StuI AGGCCT 3 cut(s) 1131, 2755, 3136
StyI CCWWGG 2 cut(s) 383, 2217
TaiI ACGT 3 cut(s) 1094, 2684, 2937
TaqI TCGA 7 cut(s) 146, 1392, 1631, 1870, 2317, 2377, 3163
TaqII GACCGA 3 cut(s) 106, 263, 3536
TatI WGTACW 3 cut(s) 505, 1365, 3115
TauI GCSGC 2 cut(s) 2275, 3175
TfiI GAWTC 7 cut(s) 1003, 1028, 1580, 2009, 2353, 2491, 3319
Tru1I TTAA 9 cut(s) 630, 711, 1122, 1647, 1755, 2483, 2604, 2780, 3351
Tru9I TTAA 9 cut(s) 630, 711, 1122, 1647, 1755, 2483, 2604, 2780, 3351
TseFI GTSAC 8 cut(s) 78, 232, 274, 399, 1655, 2598, 2666, 3308
Tsp45I GTSAC 8 cut(s) 78, 232, 274, 399, 1655, 2598, 2666, 3308
TspGWI ACGGA 3 cut(s) 2562, 2873, 3305
TspMI CCCGGG 1 cut(s) 634
Vha464I CTTAAG 1 cut(s) 2603
VneI GTGCAC 2 cut(s) 157, 330
VpaK11BI GGWCC 3 cut(s) 200, 1313, 2813
XagI CCTNNNNNAGG 2 cut(s) 2823, 3149
XapI RAATTY 8 cut(s) 142, 743, 1143, 2002, 2556, 2627, 3062, 3190
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 4 cut(s) 628, 1102, 1262, 3386
XmaI CCCGGG 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 1268
XmnI GAANNNNTTC 2 cut(s) 1131, 2286
XspI CTAG 5 cut(s) 938, 1032, 2387, 2664, 2973
ZrmI AGTACT 2 cut(s) 507, 1367
Zsp2I ATGCAT 1 cut(s) 2417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.