MD14G1158300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004286
Physical Location & Seq
Forward (+)
25238802 .. 25249618
10817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1158300.v1.1.491

Sequence Viewer

Length: 3603 bp
ATGGTTTCAACGAGGCGAAGTGGATCTCTCTCCGGAAACAACAGCAAGCGATCGTCGTCTTCCGAGGACAAGCCCCCGTCGCCGAAACGACAGAAGGCGGATAATGGAAGTGCTTCGGACAAAGCGACTCCCGGGGTGGAGAACTCGAAGGAGCTGTGTACGCCTCCGCCGGCGGCGGCTGCCGATCCCGGAGAATGTGGTCCCGACGATTCTCCGCCCGCCGGAGACGGCGTGACTTCTGGAAAGACCGAGGCTACTGCGCCAGATGTGGCGGTGGCCGCGCCGATCGCGGAAGGGTCTTCACCGGTGGTGGAAAAGCAGCGGAGGAGTTCACTCTCTTCCTGGAGTTTCTATCAGAAGCAGAGTCCGAGTTTGGATACGCCTTGGTGCAAGCTTTTGTCACAGTCTGCACTGAATGTAAATATTTCTATTAGCGCAATGACTTTCACAATTGGTGCAAACAGACAGTGCAATTTTGCTCTGAAGGATCACACCATTAGTGGGGTTCTGTGCAAGATCAAGCGCACGCAGCGCGAGGGCGGTGCTGTAGCAATTCTTGAAAGTACGGGAAGCAAGGGATCAGTGCAAGTAAATGGGACAAATGTCAAGAAAGGCAACAGTTGCGCGCTTAACCCTGGTGATGAAGTTGTCTTTGGTTCGGTGGGCAACCATGCTTATATTTTTCAGCTACTGCTGACTGAGGCTGCAGTTAAGGGTGCAGAGGTTCAAGGCAGTATTGGAAAATTTTTGCATCTTGAAAGGAGGGCAGGAGATCCTTCAGCTGTGGCTGGGGCTTCCATACTGGCTTCTCTTAGCGCGAGGGCAGAACAATCGCGATGGAAGTCCGCAGCTCAGACCACCAGTAAAGTTCACCCCGGTGCTGAGGTACCAGCTCAGTCTGTCATTGAAGATGGTACAGAAGTTGAGCTTGATGGCCTAGAAAGCAGCTCAACTCCAGACAGAGTGACTGACAAAGCTGAAGATATCAGAGCAATAGACAAGATTCTTACTCCAGACTGCAACCCCGATTCTAGCATAGAGGTATTGGAAGAAAGAAACGAGTGGACGAGGGATTCGCAATCAGCATCAACGTCAGGCATGTCTCTTCGGTGTGTTGCATTTAAAGAAGGCCTTCATGCAGGAATTCTTGATGGCAAAAGCATAGATGTCTCCTTTGATAACTTCCCATACTATTTGAGTGAGAATACGAAGAAAGTCTTGATTGCAGCCTCATTTATACACTTGAAACATAAAGAGCATGTGAAGTATACTTCAGAGCTTACAGCTGTGAACCCTCGCATTTTGCTCTCTGGTCCTTCAGGGTCTGAGATATATCAGGAGATGTTGGCAAAGGCTCTTGCGCAGTATTTTGGGGCTAAGTTGCTCATATTTGATAGCCACTCTTTTTTGGGTGGTTTATCATCAAAGGAAGCTGAGCTGATCAAGGATGGATTGAATGCAGAAAAACTCTGCAGCTTGACCAAACAAAGTCCTGCACTGCCAGACTTGGCTAAGAACAATGATCTGTCAGCTTCTGAAACAGAGGCACCAGGATCATCAAATGCACCTTTGAATGACATGGAATCCCAACCAAAGATGGAGACTGACACCCTCCCCTCTTCCTCAGGGACATCGAAGAATTACTTGTTTAAAATAGGTGACAGAGTAAGATTTAGTGCTTCATCTTCTGTTGCCTTGTATACTACATCATCTTCTTCACGGGGCCCAGTGAGCGGAATGCGCGGAGAGGTTGTGCTGCTTTTTGAGGAAAATCCTTTTTCAAAAGTCGGTGTAAAGTTTGATAAACCAATACCTGATGGTGTTGATCTGGGGGGTCTATGTAAAGGCAATGGGTACTTCTGCAATGTCTCTGATCTTCGATTGGAAACCCCGGGCACAGAAGATTTGGACAAATTACTTATCAACACATTGTTTGAGGCTGTACTAAGTGAGAGCAGAAATTCCCCTTTCATTTTGTTCATGAAAGATGCTGAGAAGTCCCTGGTGGGAAATTCCGATTCATTTTCCACATTCAGAGCCAGACTCGATAAGCTTCCAGATAATGTGGTTGTAATTGGTTCTCATACTCAAACTGACAGTCGCAAGGAGAAGTCGCACCCTGGCGGTTTGCTTTTCACAAAGTTTGGCAGCAATCAAACTGCTCTTCTTGACTTGGCTTTCCCGGATAGTTTTGGAAGACTACATGAGAGAGGGAAAGAAGTTCCCAAGGCGACAAAACTTCTGACTAAACTCTTTCCCAATAAAGTTACCATTCATATGCCGCAGGATGAAGTACTTCTTACCTCGTGGAAGCAACAATTGGATCGAGACGTTGAAACCCTAAAAATGAAGGGAAACTTGAATCACTTGCGTACTGTTCTGGGTCGATGTGGACTGGAATGTGAAGGACTTGGGACACTATGCATCAAGGATCAAACACTTACAAACGAAAGTTCAGACAAGATAGTTGGATGGGCTTTAAACCATCATTTAATGCAGAATCCTGAAGCTGATCCAGAAACAAAAGTTGTTCTGTCTGCGGAGAGCATCCAGTACGGACTTGAAATTTTACAGGCTCTCCAGAATGAAACGAAGAGTTTGAAGTCACTTAAGGATGTTGTTACGGAAAATGAATTTGAGAAAAGGCTTTTAGCTGATGTTATTCCACCTAGTGACATTGGAGTTACATTTGATGATATTGGCGCCCTTGAGAATGTGAAGGATACATTGAAGGAGTTGGTGATGCTTCCCTTACAAAGGCCAGAGCTTTTCTGCAAGGGGCAATTGACCAAGCCTTGCAAGGGTATCCTTTTATTTGGTCCCCCTGGAACAGGCAAGACAATGCTTGCAAAGGCTGTGGCTACGGAAGCTGGTGCAAACTTTATCAATATATCCATGTCGAGCATCACATCAAAGTGGTTTGGTGAGGGTGAGAAATATGTGAAAGCTGTTTTTTCGCTGGCCAGTAAAATTGCGCCTAGTGTTGTGTTCGTAGATGAAGTGGACAGTATGTTGGGCCGACGGGAAAATCCAGGTGAGCATGAGGCAATGCGTAAGATGAAGAATGAATTCATGGTAAATTGGGATGGTTTACGAACAAAAGAAGCAGAGCGAGTTCTTGTACTGGCAGCCACTAATAGGCCTTTTGACCTTGATGAGGCTGTCATTCGAAGACTGCCGCGCAGGTTGATGGTAAATTTGCCAGATGCTCCAAATAGGGCAAAAATATTGAAAGTCATACTGGCAAAAGAGGACTTGTCTCCCACTGTTGATTTTGATGCGATTGCAAGCATGACGGATGGGTATTCAGGAAGTGACCTCAAGAATCTTTGTGTAACTGCCGCACATCGTCCAATTAAAGAGATTTTGGAGAAGGAAAAAAAGGAGCATGCTGTAGCCGTAGCGGAAGGTAAACCCGTTCCAGCTTTGAGTGGCAGTGCTGATATACGGCCTCTGAACATGGATGACTTCAAAGATGCTCACGAACGGGTATGCGCTAGTGTTTCATCGGAATCCGTGAACATGACCGAGCTTTTACAATGGAACGAACTCTATGGCGAAGGGGGCTCAAGAAGAAAGAAGGCCCTTAGCTACTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

1201

Amino Acids

129.8

Weight (kDa)

5.89

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 150 - 219 1.1e-06 FHA domain
DUF7751 PF24933 799 - 858 1.5e-19 Domain of unknown function (DUF7751)
AAA PF00004 936 - 1066 1.4e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 1090 - 1129 6.7e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1362
Acc36I ACCTGC 1 cut(s) 3174
Acc65I GGTACC 1 cut(s) 886
AccB1I GGYRCC 3 cut(s) 886, 1546, 2702
AccBSI CCGCTC 1 cut(s) 1734
AccI GTMKAC 2 cut(s) 1268, 1700
AccII CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
AccIII TCCGGA 1 cut(s) 32
AclWI GGATC 9 cut(s) 31, 179, 495, 586, 767, 1561, 2331, 2439, 2507
AcoI YGGCCR 2 cut(s) 276, 2961
AcsI RAATTY 8 cut(s) 743, 1143, 1960, 2011, 2565, 2633, 3068, 3196
AcuI CTGAAG 6 cut(s) 503, 762, 999, 1257, 1302, 2526
AcyI GRCGYC 1 cut(s) 2703
AdeI CACNNNGTG 1 cut(s) 2672
AfiI CCNNNNNNNGG 8 cut(s) 221, 501, 1733, 2757, 2801, 2831, 3138, 3157
AflII CTTAAG 1 cut(s) 2609
AgeI ACCGGT 1 cut(s) 304
AjnI CCWGG 7 cut(s) 341, 634, 1549, 2001, 2119, 2824, 3031
AjuI GAANNNNNNNTTGG 4 cut(s) 636, 668, 2303, 2335
AleI CACNNNNGTG 1 cut(s) 876
Alw26I GTCTC 7 cut(s) 219, 1107, 1174, 1595, 1873, 2322, 3264
AlwI GGATC 9 cut(s) 31, 179, 495, 586, 767, 1561, 2331, 2439, 2507
AlwNI CAGNNNCTG 3 cut(s) 691, 1325, 1535
Ama87I CYCGRG 2 cut(s) 131, 1891
Aor13HI TCCGGA 1 cut(s) 32
ApaI GGGCCC 1 cut(s) 1727
ApoI RAATTY 8 cut(s) 743, 1143, 1960, 2011, 2565, 2633, 3068, 3196
ArsI GACNNNNNNTTYG 4 cut(s) 1057, 1089, 2083, 2115
AsiGI ACCGGT 1 cut(s) 304
Asp700I GAANNNNTTC 4 cut(s) 112, 1131, 2295, 3068
Asp718I GGTACC 1 cut(s) 886
AspS9I GGNCC 7 cut(s) 200, 1313, 1723, 1724, 2819, 3016, 3583
AsuC2I CCSGG 7 cut(s) 132, 133, 189, 876, 1892, 1893, 2183
AsuHPI GGTGA 8 cut(s) 294, 650, 863, 1670, 2752, 2936, 2942, 3047
AsuII TTCGAA 1 cut(s) 3169
AvaI CYCGRG 2 cut(s) 131, 1891
AvaII GGWCC 3 cut(s) 200, 1313, 2819
AxyI CCTNAGG 1 cut(s) 1624
BaeGI GKGCMC 2 cut(s) 1727, 1898
BaeI ACNNNNGTAYC 2 cut(s) 906, 939
BalI TGGCCA 1 cut(s) 2963
BanI GGYRCC 3 cut(s) 886, 1546, 2702
BanII GRGCYC 2 cut(s) 1727, 3569
BauI CACGAG 1 cut(s) 2305
BbsI GAAGAC 4 cut(s) 51, 291, 2203, 3178
BbvCI CCTCAGC 1 cut(s) 882
BceAI ACGGC 3 cut(s) 244, 3383, 3464
BcgI CGANNNNNNTGC 6 cut(s) 239, 273, 524, 558, 813, 847
BciT130I CCWGG 7 cut(s) 343, 636, 1551, 2003, 2121, 2826, 3033
BciVI GTATCC 3 cut(s) 370, 2716, 2816
BclI TGATCA 1 cut(s) 1440
BcnI CCSGG 7 cut(s) 132, 133, 189, 876, 1892, 1893, 2183
BcoDI GTCTC 7 cut(s) 219, 1107, 1174, 1595, 1873, 2322, 3264
BfaI CTAG 5 cut(s) 938, 1032, 2670, 2979, 3498
BfmI CTRYAG 4 cut(s) 546, 705, 1471, 3393
BfoI RGCGCY 1 cut(s) 2706
BfrI CTTAAG 1 cut(s) 2609
BfuAI ACCTGC 1 cut(s) 3174
BfuI GTATCC 3 cut(s) 370, 2716, 2816
BlpI GCTNAGC 1 cut(s) 1434
BmcAI AGTACT 1 cut(s) 2295
Bme18I GGWCC 3 cut(s) 200, 1313, 2819
BmeT110I CYCGRG 2 cut(s) 131, 1891
BmgT120I GGNCC 7 cut(s) 200, 1313, 1723, 1724, 2819, 3016, 3583
BmiI GGNNCC 7 cut(s) 202, 888, 1548, 1724, 1725, 2704, 2821
BmrI ACTGGG 1 cut(s) 1721
BmuI ACTGGG 1 cut(s) 1721
BoxI GACNNNNGTC 1 cut(s) 602
BpiI GAAGAC 4 cut(s) 51, 291, 2203, 3178
BplI GAGNNNNNCTC 2 cut(s) 2028, 2060
BpmI CTGGAG 4 cut(s) 364, 939, 996, 2564
Bpu10I CCTNAGC 2 cut(s) 882, 3587
Bpu1102I GCTNAGC 1 cut(s) 1434
Bpu14I TTCGAA 1 cut(s) 3169
BpuEI CTTGAG 3 cut(s) 2729, 3305, 3553
BpuMI CCSGG 7 cut(s) 132, 133, 189, 876, 1892, 1893, 2183
BsaHI GRCGYC 1 cut(s) 2703
BsaWI WCCGGW 2 cut(s) 32, 304
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 1737, 1767, 2562, 2592
Bsc4I CCNNNNNNNGG 8 cut(s) 221, 501, 1733, 2757, 2801, 2831, 3138, 3157
Bse118I RCCGGY 2 cut(s) 169, 304
Bse1I ACTGG 8 cut(s) 807, 861, 1727, 2400, 2551, 2964, 3129, 3246
Bse21I CCTNAGG 1 cut(s) 1624
Bse3DI GCAATG 4 cut(s) 444, 1855, 1870, 3054
BseAI TCCGGA 1 cut(s) 32
BseBI CCWGG 7 cut(s) 343, 636, 1551, 2003, 2121, 2826, 3033
BseGI GGATG 8 cut(s) 1453, 2293, 2477, 2547, 2620, 3091, 3304, 3469
BseLI CCNNNNNNNGG 8 cut(s) 221, 501, 1733, 2757, 2801, 2831, 3138, 3157
BseMI GCAATG 4 cut(s) 444, 1855, 1870, 3054
BseMII CTCAG 8 cut(s) 690, 866, 873, 908, 1317, 1425, 1638, 1983
BseNI ACTGG 8 cut(s) 807, 861, 1727, 2400, 2551, 2964, 3129, 3246
BsePI GCGCGC 1 cut(s) 624
BseRI GAGGAG 1 cut(s) 340
BseSI GKGCMC 2 cut(s) 1727, 1898
BseYI CCCAGC 1 cut(s) 788
BsgI GTGCAG 3 cut(s) 393, 738, 1479
Bsh1236I CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
Bsh1285I CGRYCG 2 cut(s) 53, 288
BshNI GGYRCC 3 cut(s) 886, 1546, 2702
BshTI ACCGGT 1 cut(s) 304
BsiEI CGRYCG 2 cut(s) 53, 288
BsiHKCI CYCGRG 2 cut(s) 131, 1891
BsiSI CCGG 9 cut(s) 33, 132, 170, 189, 222, 305, 876, 1892, 2183
BslFI GGGAC 6 cut(s) 186, 610, 1642, 1984, 2428, 2805
BslI CCNNNNNNNGG 8 cut(s) 221, 501, 1733, 2757, 2801, 2831, 3138, 3157
BsmAI GTCTC 7 cut(s) 219, 1107, 1174, 1595, 1873, 2322, 3264
BsmBI CGTCTC 2 cut(s) 219, 2322
BsmFI GGGAC 6 cut(s) 186, 610, 1642, 1984, 2428, 2805
BsmI GAATGC 2 cut(s) 1462, 1743
BsoBI CYCGRG 2 cut(s) 131, 1891
Bsp119I TTCGAA 1 cut(s) 3169
Bsp120I GGGCCC 1 cut(s) 1723
Bsp1286I GDGCHC 3 cut(s) 1727, 1898, 3569
Bsp13I TCCGGA 1 cut(s) 32
Bsp1720I GCTNAGC 1 cut(s) 1434
Bsp68I TCGCGA 1 cut(s) 835
BspCNI CTCAG 8 cut(s) 691, 865, 874, 907, 1318, 1426, 1637, 1984
BspEI TCCGGA 1 cut(s) 32
BspFNI CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
BspHI TCATGA 1 cut(s) 1980
BspLI GGNNCC 7 cut(s) 202, 888, 1548, 1724, 1725, 2704, 2821
BspMAI CTGCAG 2 cut(s) 709, 1475
BspMI ACCTGC 1 cut(s) 3174
BspPI GGATC 9 cut(s) 31, 179, 495, 586, 767, 1561, 2331, 2439, 2507
BspQI GCTCTTC 1 cut(s) 2169
BspT104I TTCGAA 1 cut(s) 3169
BspT107I GGYRCC 3 cut(s) 886, 1546, 2702
BspTI CTTAAG 1 cut(s) 2609
BsrBI CCGCTC 1 cut(s) 1734
BsrDI GCAATG 4 cut(s) 444, 1855, 1870, 3054
BsrFI RCCGGY 2 cut(s) 169, 304
BsrI ACTGG 8 cut(s) 807, 861, 1727, 2400, 2551, 2964, 3129, 3246
BssAI RCCGGY 2 cut(s) 169, 304
BssHII GCGCGC 1 cut(s) 624
BssNAI GTATAC 2 cut(s) 1269, 1701
BssNI GRCGYC 1 cut(s) 2703
BssSI CACGAG 1 cut(s) 2305
BssT1I CCWWGG 2 cut(s) 383, 2226
Bst1107I GTATAC 2 cut(s) 1269, 1701
Bst2BI CACGAG 1 cut(s) 2305
Bst2UI CCWGG 7 cut(s) 343, 636, 1551, 2003, 2121, 2826, 3033
Bst4CI ACNGT 7 cut(s) 405, 468, 620, 2099, 2377, 3008, 3268
Bst6I CTCTTC 5 cut(s) 343, 1110, 1624, 2169, 2588
BstACI GRCGYC 1 cut(s) 2703
BstAFI CTTAAG 1 cut(s) 2609
BstAPI GCANNNNNTGC 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 3169
BstENI CCTNNNNNAGG 3 cut(s) 2755, 2829, 3155
BstF5I GGATG 8 cut(s) 1453, 2293, 2477, 2547, 2620, 3091, 3304, 3469
BstFNI CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
BstH2I RGCGCY 1 cut(s) 2706
BstMAI GTCTC 7 cut(s) 219, 1107, 1174, 1595, 1873, 2322, 3264
BstMCI CGRYCG 2 cut(s) 53, 288
BstNI CCWGG 7 cut(s) 343, 636, 1551, 2003, 2121, 2826, 3033
BstNSI RCATGY 3 cut(s) 1102, 1262, 3392
BstPAI GACNNNNGTC 1 cut(s) 602
BstSFI CTRYAG 4 cut(s) 546, 705, 1471, 3393
BstSLI GKGCMC 2 cut(s) 1727, 1898
BstUI CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
BstV2I GAAGAC 4 cut(s) 51, 291, 2203, 3178
BstX2I RGATCY 2 cut(s) 23, 772
BstYI RGATCY 2 cut(s) 23, 772
BstZ17I GTATAC 2 cut(s) 1269, 1701
Bsu36I CCTNAGG 1 cut(s) 1624
BsuI GTATCC 3 cut(s) 370, 2716, 2816
BtgZI GCGATG 1 cut(s) 850
BtsCI GGATG 8 cut(s) 1453, 2293, 2477, 2547, 2620, 3091, 3304, 3469
BtsI GCAGTG 2 cut(s) 1496, 3442
BtsIMutI CAGTG 7 cut(s) 410, 473, 588, 1496, 1734, 3264, 3442
BtuMI TCGCGA 1 cut(s) 835
BveI ACCTGC 1 cut(s) 3174
CaiI CAGNNNCTG 3 cut(s) 691, 1325, 1535
CciI TCATGA 1 cut(s) 1980
Cfr10I RCCGGY 2 cut(s) 169, 304
Cfr13I GGNCC 7 cut(s) 200, 1313, 1723, 1724, 2819, 3016, 3583
Cfr9I CCCGGG 2 cut(s) 131, 1891
CspAI ACCGGT 1 cut(s) 304
CspCI CAANNNNNGTGG 2 cut(s) 2838, 2873
DinI GGCGCC 1 cut(s) 2704
DraI TTTAAA 3 cut(s) 1123, 1651, 2481
DraIII CACNNNGTG 1 cut(s) 2672
EaeI YGGCCR 2 cut(s) 276, 2961
Eam1104I CTCTTC 5 cut(s) 343, 1110, 1624, 2169, 2588
EarI CTCTTC 5 cut(s) 343, 1110, 1624, 2169, 2588
EciI GGCGGA 3 cut(s) 113, 156, 204
Eco130I CCWWGG 2 cut(s) 383, 2226
Eco147I AGGCCT 2 cut(s) 1131, 3142
Eco24I GRGCYC 2 cut(s) 1727, 3569
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 3 cut(s) 200, 1313, 2819
Eco57I CTGAAG 6 cut(s) 503, 762, 999, 1257, 1302, 2526
Eco81I CCTNAGG 1 cut(s) 1624
Eco88I CYCGRG 2 cut(s) 131, 1891
EcoNI CCTNNNNNAGG 3 cut(s) 2755, 2829, 3155
EcoO109I RGGNCCY 2 cut(s) 1723, 3583
EcoRI GAATTC 2 cut(s) 1143, 3068
EcoRII CCWGG 7 cut(s) 341, 634, 1549, 2001, 2119, 2824, 3031
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 383, 2226
EcoT22I ATGCAT 1 cut(s) 2426
EcoT38I GRGCYC 2 cut(s) 1727, 3569
EgeI GGCGCC 1 cut(s) 2704
EheI GGCGCC 1 cut(s) 2704
ErhI CCWWGG 2 cut(s) 383, 2226
Esp3I CGTCTC 2 cut(s) 219, 2322
FaqI GGGAC 6 cut(s) 186, 610, 1642, 1984, 2428, 2805
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 1 cut(s) 2277
FbaI TGATCA 1 cut(s) 1440
FblI GTMKAC 2 cut(s) 1268, 1700
FokI GGATG 8 cut(s) 1460, 2300, 2484, 2534, 2627, 3098, 3311, 3476
FriOI GRGCYC 2 cut(s) 1727, 3569
FspBI CTAG 5 cut(s) 938, 1032, 2670, 2979, 3498
FspI TGCGCA 1 cut(s) 1362
GsaI CCCAGC 1 cut(s) 792
GsuI CTGGAG 4 cut(s) 364, 939, 996, 2564
HaeII RGCGCY 1 cut(s) 2706
HapII CCGG 9 cut(s) 33, 132, 170, 189, 222, 305, 876, 1892, 2183
Hin1I GRCGYC 1 cut(s) 2703
HindIII AAGCTT 2 cut(s) 392, 2051
HpaII CCGG 9 cut(s) 33, 132, 170, 189, 222, 305, 876, 1892, 2183
HphI GGTGA 8 cut(s) 294, 650, 863, 1670, 2752, 2936, 2942, 3047
Hpy99I CGWCG 4 cut(s) 58, 82, 209, 3024
HpyCH4III ACNGT 7 cut(s) 405, 468, 620, 2099, 2377, 3008, 3268
HpyCH4IV ACGT 2 cut(s) 1091, 2331
HpySE526I ACGT 2 cut(s) 1091, 2331
Hsp92I GRCGYC 1 cut(s) 2703
KasI GGCGCC 1 cut(s) 2702
Kpn2I TCCGGA 1 cut(s) 32
KpnI GGTACC 1 cut(s) 890
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 1440
LguI GCTCTTC 1 cut(s) 2169
LmnI GCTCC 3 cut(s) 151, 3214, 3385
MaeI CTAG 5 cut(s) 938, 1032, 2670, 2979, 3498
MaeII ACGT 2 cut(s) 1091, 2331
MbiI CCGCTC 1 cut(s) 1734
MfeI CAATTG 3 cut(s) 450, 2318, 2783
MflI RGATCY 2 cut(s) 23, 772
MhlI GDGCHC 3 cut(s) 1727, 1898, 3569
MlsI TGGCCA 1 cut(s) 2963
MluNI TGGCCA 1 cut(s) 2963
Mly113I GGCGCC 1 cut(s) 2703
MlyI GAGTC 3 cut(s) 121, 373, 2037
MmeI TCCRAC 1 cut(s) 2449
Mox20I TGGCCA 1 cut(s) 2963
Mph1103I ATGCAT 1 cut(s) 2426
MreI CGCCGGCG 1 cut(s) 169
MroI TCCGGA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 169
MroXI GAANNNNTTC 4 cut(s) 112, 1131, 2295, 3068
MscI TGGCCA 1 cut(s) 2963
MseI TTAA 8 cut(s) 630, 711, 1122, 1650, 2480, 2492, 2610, 3357
MslI CAYNNNNRTG 3 cut(s) 876, 2276, 2914
Msp20I TGGCCA 1 cut(s) 2963
MspA1I CMGCKG 3 cut(s) 322, 782, 1286
MspCI CTTAAG 1 cut(s) 2609
MspI CCGG 9 cut(s) 33, 132, 170, 189, 222, 305, 876, 1892, 2183
MunI CAATTG 3 cut(s) 450, 2318, 2783
Mva1269I GAATGC 2 cut(s) 1462, 1743
MvaI CCWGG 7 cut(s) 343, 636, 1551, 2003, 2121, 2826, 3033
MvnI CGCG 8 cut(s) 281, 290, 534, 626, 818, 835, 1743, 3181
NaeI GCCGGC 1 cut(s) 171
NarI GGCGCC 1 cut(s) 2703
NciI CCSGG 7 cut(s) 132, 133, 189, 876, 1892, 1893, 2183
NdeI CATATG 1 cut(s) 2277
NgoMIV GCCGGC 1 cut(s) 169
NlaIV GGNNCC 7 cut(s) 202, 888, 1548, 1724, 1725, 2704, 2821
NmuCI GTSAC 7 cut(s) 232, 399, 964, 1658, 2604, 2672, 3314
NruI TCGCGA 1 cut(s) 835
NsbI TGCGCA 1 cut(s) 1362
NsiI ATGCAT 1 cut(s) 2426
NspI RCATGY 3 cut(s) 1102, 1262, 3392
NspV TTCGAA 1 cut(s) 3169
OliI CACNNNNGTG 1 cut(s) 876
PaeI GCATGC 1 cut(s) 3392
PagI TCATGA 1 cut(s) 1980
PauI GCGCGC 1 cut(s) 624
PceI AGGCCT 2 cut(s) 1131, 3142
PciSI GCTCTTC 1 cut(s) 2169
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 2 cut(s) 1462, 1743
PdiI GCCGGC 1 cut(s) 171
PdmI GAANNNNTTC 4 cut(s) 112, 1131, 2295, 3068
PfoI TCCNGGA 3 cut(s) 187, 341, 2181
PinAI ACCGGT 1 cut(s) 304
Ple19I CGATCG 2 cut(s) 53, 288
PleI GAGTC 3 cut(s) 121, 372, 2037
PluTI GGCGCC 1 cut(s) 2706
PpsI GAGTC 3 cut(s) 121, 372, 2037
PshAI GACNNNNGTC 1 cut(s) 602
Psp6I CCWGG 7 cut(s) 341, 634, 1549, 2001, 2119, 2824, 3031
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 7 cut(s) 341, 634, 1549, 2001, 2119, 2824, 3031
PspN4I GGNNCC 7 cut(s) 202, 888, 1548, 1724, 1725, 2704, 2821
PspOMI GGGCCC 1 cut(s) 1723
PspPI GGNCC 7 cut(s) 200, 1313, 1723, 1724, 2819, 3016, 3583
PstI CTGCAG 2 cut(s) 709, 1475
PstNI CAGNNNCTG 3 cut(s) 691, 1325, 1535
PsuI RGATCY 2 cut(s) 23, 772
PteI GCGCGC 1 cut(s) 624
PvuI CGATCG 2 cut(s) 53, 288
PvuII CAGCTG 2 cut(s) 782, 1286
RruI TCGCGA 1 cut(s) 835
RseI CAYNNNNRTG 3 cut(s) 876, 2276, 2914
SapI GCTCTTC 1 cut(s) 2169
SaqAI TTAA 8 cut(s) 630, 711, 1122, 1650, 2480, 2492, 2610, 3357
Sau96I GGNCC 7 cut(s) 200, 1313, 1723, 1724, 2819, 3016, 3583
ScaI AGTACT 1 cut(s) 2295
SchI GAGTC 3 cut(s) 121, 373, 2037
SduI GDGCHC 3 cut(s) 1727, 1898, 3569
SfcI CTRYAG 4 cut(s) 546, 705, 1471, 3393
SfoI GGCGCC 1 cut(s) 2704
SfuI TTCGAA 1 cut(s) 3169
SgrAI CRCCGGYG 2 cut(s) 169, 304
SinI GGWCC 3 cut(s) 200, 1313, 2819
SmaI CCCGGG 2 cut(s) 133, 1893
SmiMI CAYNNNNRTG 3 cut(s) 876, 2276, 2914
SmlI CTYRAG 4 cut(s) 2609, 2708, 3320, 3568
SmoI CTYRAG 4 cut(s) 2609, 2708, 3320, 3568
SphI GCATGC 1 cut(s) 3392
SseBI AGGCCT 2 cut(s) 1131, 3142
SspDI GGCGCC 1 cut(s) 2702
SspI AATATT 2 cut(s) 424, 3228
SspMI CTAG 5 cut(s) 938, 1032, 2670, 2979, 3498
StuI AGGCCT 2 cut(s) 1131, 3142
StyI CCWWGG 2 cut(s) 383, 2226
TaaI ACNGT 7 cut(s) 405, 468, 620, 2099, 2377, 3008, 3268
TaiI ACGT 2 cut(s) 1094, 2334
TaqI TCGA 8 cut(s) 146, 1634, 1879, 2046, 2326, 2386, 2900, 3169
TaqII GACCGA 2 cut(s) 263, 3542
TatI WGTACW 3 cut(s) 1942, 2293, 3121
TauI GCSGC 6 cut(s) 176, 179, 281, 2284, 3181, 3344
Tru1I TTAA 8 cut(s) 630, 711, 1122, 1650, 2480, 2492, 2610, 3357
Tru9I TTAA 8 cut(s) 630, 711, 1122, 1650, 2480, 2492, 2610, 3357
TscAI CASTG 7 cut(s) 417, 473, 588, 1503, 1734, 3271, 3442
TseFI GTSAC 7 cut(s) 232, 399, 964, 1658, 2604, 2672, 3314
Tsp45I GTSAC 7 cut(s) 232, 399, 964, 1658, 2604, 2672, 3314
TspGWI ACGGA 5 cut(s) 2571, 2639, 2879, 3311, 3505
TspMI CCCGGG 2 cut(s) 131, 1891
TspRI CASTG 7 cut(s) 417, 473, 588, 1503, 1734, 3271, 3442
Vha464I CTTAAG 1 cut(s) 2609
VpaK11BI GGWCC 3 cut(s) 200, 1313, 2819
XagI CCTNNNNNAGG 3 cut(s) 2755, 2829, 3155
XapI RAATTY 8 cut(s) 743, 1143, 1960, 2011, 2565, 2633, 3068, 3196
XceI RCATGY 3 cut(s) 1102, 1262, 3392
XmaI CCCGGG 2 cut(s) 131, 1891
XmiI GTMKAC 2 cut(s) 1268, 1700
XmnI GAANNNNTTC 4 cut(s) 112, 1131, 2295, 3068
XspI CTAG 5 cut(s) 938, 1032, 2670, 2979, 3498
ZrmI AGTACT 1 cut(s) 2295
Zsp2I ATGCAT 1 cut(s) 2426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.