pycom14g13190

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004286
Physical Location & Seq
Forward (+)
16173849 .. 16175121
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g13190.4

Sequence Viewer

Length: 597 bp
ATGGTTTCAACGAGGCGAAGTGGATCTCTCTCCGGAAACAACAGCAAACGATCGTCGTCTTCCGAGGACAAGCCCCCGTCGCCGAAACGACAGAAGGCGGATAATGGAAGTGCTTCGGAGAAAGCCACTCCCGGGGTGGAGAATTCGAAGGAGCTGTGTACGCCTCCGCCGGCGGCGGCTGCCGATCCCGGAGAATGTGGTCCCGACTATTCTCCGCCCGCCGGAGACGGCGTGACTTCTGGAAAGACCGAAGCTACTGCGCCAGATGTGGCGGTGGCGGCGCCGATCGCGGAAGGGTCTTCGCCGGTGGTGGAAAAGCAGCCGAGGAGTTCACTCTCCTATAGTTTCTATCAGAAGCAGAGTCCGAGTTTCGATACGCCTTGGTGCAAGCTTTTGTCACAGTCTGCACTGAATGTAAATATTTCTATTAGCGCAATGACTTTCACAATTGGTGCAGACAGACAGTGCAATTTTGCTCTGAGGGGTCACACCATTAGTGGGGTTCTGTGCAAGATCAAGCGCACACAGCGCGATATCGGTGCTGTAGCAATTCTTGAAAATATGGGAAGCTTAGCAAGGATCGATCAGTGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FHA

Protein Analysis

199

Amino Acids

20.69

Weight (kDa)

8.75

Isoelectric Point (pI)

62.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccII CGCG 2 cut(s) 290, 531
AccIII TCCGGA 1 cut(s) 32
AciI CCGC 9 cut(s) 98, 167, 173, 176, 215, 219, 272, 278, 290
AclWI GGATC 3 cut(s) 31, 179, 587
AcsI RAATTY 1 cut(s) 142
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 3 cut(s) 132, 221, 498
AgsI TTSAA 2 cut(s) 9, 557
AluBI AGCT 4 cut(s) 154, 254, 391, 570
AluI AGCT 4 cut(s) 154, 254, 391, 570
Alw26I GTCTC 1 cut(s) 219
AlwI GGATC 3 cut(s) 31, 179, 587
Ama87I CYCGRG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 32
ApeKI GCWGC 2 cut(s) 179, 319
ApoI RAATTY 1 cut(s) 142
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 5 cut(s) 262, 283, 434, 522, 531
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 3 cut(s) 132, 133, 189
AsuII TTCGAA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 280
BbsI GAAGAC 2 cut(s) 51, 291
BbvI GCAGC 2 cut(s) 166, 331
BceAI ACGGC 1 cut(s) 244
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 521, 555
BcnI CCSGG 3 cut(s) 132, 133, 189
BcoDI GTCTC 1 cut(s) 219
BfmI CTRYAG 2 cut(s) 340, 543
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 5 cut(s) 174, 177, 180, 279, 320
BlpI GCTNAGC 1 cut(s) 571
BlsI GCNGC 5 cut(s) 175, 178, 181, 280, 321
Bme1390I CCNGG 3 cut(s) 132, 133, 189
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 2 cut(s) 202, 282
BmrFI CCNGG 3 cut(s) 132, 133, 189
BpiI GAAGAC 2 cut(s) 51, 291
Bpu1102I GCTNAGC 1 cut(s) 571
Bpu14I TTCGAA 1 cut(s) 146
BpuMI CCSGG 3 cut(s) 132, 133, 189
Bsa29I ATCGAT 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 5 cut(s) 63, 131, 132, 323, 380
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 221, 498
Bse118I RCCGGY 2 cut(s) 169, 304
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 5 cut(s) 63, 131, 132, 323, 380
BseLI CCNNNNNNNGG 3 cut(s) 132, 221, 498
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 340
BseXI GCAGC 2 cut(s) 166, 331
BsgI GTGCAG 2 cut(s) 390, 474
Bsh1236I CGCG 2 cut(s) 290, 531
Bsh1285I CGRYCG 2 cut(s) 53, 288
BshNI GGYRCC 1 cut(s) 280
BshVI ATCGAT 1 cut(s) 582
BsiEI CGRYCG 2 cut(s) 53, 288
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 6 cut(s) 33, 132, 170, 189, 222, 305
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 3 cut(s) 132, 221, 498
BsmAI GTCTC 1 cut(s) 219
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 146
Bsp13I TCCGGA 1 cut(s) 32
Bsp143I GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
Bsp1720I GCTNAGC 1 cut(s) 571
BspACI CCGC 9 cut(s) 98, 167, 173, 176, 215, 219, 272, 278, 290
BspCNI CTCAG 1 cut(s) 471
BspDI ATCGAT 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 32
BspFNI CGCG 2 cut(s) 290, 531
BspLI GGNNCC 2 cut(s) 202, 282
BspPI GGATC 3 cut(s) 31, 179, 587
BspT104I TTCGAA 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 2 cut(s) 169, 304
BssAI RCCGGY 2 cut(s) 169, 304
BssECI CCNNGG 5 cut(s) 63, 131, 132, 323, 380
BssMI GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 402, 465
BstACI GRCGYC 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 171, 219, 389
BstDEI CTNAG 2 cut(s) 479, 571
BstFNI CGCG 2 cut(s) 290, 531
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 5 cut(s) 262, 283, 434, 522, 531
BstKTI GATC 7 cut(s) 26, 53, 187, 288, 516, 582, 586
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
BstMCI CGRYCG 2 cut(s) 53, 288
BstMWI GCNNNNNNNGC 7 cut(s) 79, 160, 179, 278, 287, 526, 528
BstSCI CCNGG 3 cut(s) 130, 131, 187
BstSFI CTRYAG 2 cut(s) 340, 543
BstUI CGCG 2 cut(s) 290, 531
BstV1I GCAGC 2 cut(s) 166, 331
BstV2I GAAGAC 2 cut(s) 51, 291
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 582
BsuTUI ATCGAT 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 407, 470, 593
Cac8I GCNNGC 3 cut(s) 171, 219, 389
CfoI GCGC 5 cut(s) 262, 283, 434, 522, 531
Cfr10I RCCGGY 2 cut(s) 169, 304
Cfr13I GGNCC 1 cut(s) 200
Cfr9I CCCGGG 1 cut(s) 131
ClaI ATCGAT 1 cut(s) 582
Csp6I GTAC 1 cut(s) 159
CviJI RGCY 8 cut(s) 73, 125, 154, 179, 254, 322, 391, 570
CviKI_1 RGCY 8 cut(s) 73, 125, 154, 179, 254, 322, 391, 570
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 2 cut(s) 479, 571
DinI GGCGCC 1 cut(s) 282
DpnI GATC 7 cut(s) 25, 52, 186, 287, 515, 581, 585
DpnII GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
EciI GGCGGA 3 cut(s) 113, 156, 204
Eco130I CCWWGG 1 cut(s) 380
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 200
Eco88I CYCGRG 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 142
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 380
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 380
Esp3I CGTCTC 1 cut(s) 219
FaiI YATR 2 cut(s) 342, 563
FaqI GGGAC 1 cut(s) 186
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 5 cut(s) 174, 177, 180, 279, 320
Fsp4HI GCNGC 5 cut(s) 174, 177, 180, 279, 320
GlaI GCGC 5 cut(s) 261, 282, 433, 521, 530
GluI GCNGC 5 cut(s) 174, 177, 180, 279, 320
HaeII RGCGCY 1 cut(s) 284
HapII CCGG 6 cut(s) 33, 132, 170, 189, 222, 305
HhaI GCGC 5 cut(s) 262, 283, 434, 522, 531
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 5 cut(s) 260, 281, 432, 520, 529
HinP1I GCGC 5 cut(s) 260, 281, 432, 520, 529
HindIII AAGCTT 2 cut(s) 389, 568
HinfI GANTC 1 cut(s) 361
HpaII CCGG 6 cut(s) 33, 132, 170, 189, 222, 305
Hpy166II GTNNAC 2 cut(s) 159, 332
Hpy188I TCNGA 5 cut(s) 64, 118, 354, 366, 480
Hpy188III TCNNGA 4 cut(s) 33, 203, 240, 554
Hpy8I GTNNAC 2 cut(s) 159, 332
Hpy99I CGWCG 2 cut(s) 58, 82
HpyAV CCTTC 3 cut(s) 88, 142, 287
HpyCH4III ACNGT 2 cut(s) 402, 465
HpyCH4V TGCA 6 cut(s) 387, 407, 455, 468, 510, 591
HpyF10VI GCNNNNNNNGC 7 cut(s) 79, 160, 179, 278, 287, 526, 528
HpyF3I CTNAG 2 cut(s) 479, 571
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 5 cut(s) 260, 281, 432, 520, 529
KasI GGCGCC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 32
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Kzo9I GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 8 cut(s) 46, 145, 183, 202, 225, 235, 276, 318
Lsp1109I GCAGC 2 cut(s) 166, 331
MaeIII GTNAC 3 cut(s) 232, 396, 485
MalI GATC 7 cut(s) 25, 52, 186, 287, 515, 581, 585
MboI GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
MboII GAAGA 2 cut(s) 51, 291
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 1 cut(s) 23
MluCI AATT 4 cut(s) 142, 447, 469, 549
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 1 cut(s) 370
MnlI CCTC 5 cut(s) 6, 58, 174, 318, 474
MreI CGCCGGCG 1 cut(s) 169
MroI TCCGGA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 112
MspI CCGG 6 cut(s) 33, 132, 170, 189, 222, 305
MspR9I CCNGG 3 cut(s) 132, 133, 189
MunI CAATTG 1 cut(s) 447
MvnI CGCG 2 cut(s) 290, 531
MwoI GCNNNNNNNGC 7 cut(s) 79, 160, 179, 278, 287, 526, 528
NaeI GCCGGC 1 cut(s) 171
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 3 cut(s) 132, 133, 189
NdeII GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
NgoMIV GCCGGC 1 cut(s) 169
NlaIV GGNNCC 2 cut(s) 202, 282
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 3 cut(s) 232, 396, 485
NspV TTCGAA 1 cut(s) 146
PdiI GCCGGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 5 cut(s) 175, 178, 181, 280, 321
Ple19I CGATCG 2 cut(s) 53, 288
PleI GAGTC 1 cut(s) 369
PluTI GGCGCC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 202, 282
PspPI GGNCC 1 cut(s) 200
PsuI RGATCY 1 cut(s) 23
PvuI CGATCG 2 cut(s) 53, 288
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SatI GCNGC 5 cut(s) 174, 177, 180, 279, 320
Sau3AI GATC 7 cut(s) 23, 50, 184, 285, 513, 579, 583
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 132, 133, 189
SetI ASST 4 cut(s) 156, 256, 393, 572
SfcI CTRYAG 2 cut(s) 340, 543
SfoI GGCGCC 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 146
SgrAI CRCCGGYG 2 cut(s) 169, 304
SinI GGWCC 1 cut(s) 200
SmaI CCCGGG 1 cut(s) 133
Sse9I AATT 4 cut(s) 142, 447, 469, 549
SsiI CCGC 9 cut(s) 98, 167, 173, 176, 215, 219, 272, 278, 290
SspDI GGCGCC 1 cut(s) 280
SspI AATATT 1 cut(s) 421
StyD4I CCNGG 3 cut(s) 130, 131, 187
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 2 cut(s) 402, 465
TaqI TCGA 3 cut(s) 146, 372, 582
TaqII GACCGA 1 cut(s) 263
TasI AATT 4 cut(s) 142, 447, 469, 549
TauI GCSGC 3 cut(s) 176, 179, 281
TscAI CASTG 3 cut(s) 414, 470, 593
TseFI GTSAC 3 cut(s) 232, 396, 485
TseI GCWGC 2 cut(s) 179, 319
Tsp45I GTSAC 3 cut(s) 232, 396, 485
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 3 cut(s) 414, 470, 593
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmaI CCCGGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.