FvH4_5g22950

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011564
Physical Location & Seq
Forward (+)
14262713 .. 14264899
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22950.t1

Sequence Viewer

Length: 1521 bp
ATGCATCGGCTACGAAGCATACATATCTCAGCGAGAAACCCTTTTTCAAATAAGCTTCGCAAACAGCAAACAAGGAGATCAAAGCTCAATCCAGAGAGATCGTGCTTCCCTATTAGTACCATGGAAGGTTTGGCACGAGATGATAGCATAATTGATATTGCAGCTGTCGAAAATGGTTTGAGATCTTCCATGAGAAATGGTGATACTGCTGCACTGCCTGTCGAGAGTAGCAAGGAATCTTCTATGGACAATGTTGATGTTGCAGCTGTCGAAACTGACTTGAGATTTTGCATGGACAACATTGAGTCTGGGAACTCTCTTTTGTCAACCAAGTGTTGCATCTTTGTGACCCCCAAATTACTCTTCAGAAATAACGAAAAAGCTTTTCTCCCCACAGCATTCTCATTCGGAACATTGCACCATGGCAAACCAGACTTGGCCGACACACAGAAAATCAAACTGAAGTATGCGCGAGACCTCATCAGTCGAGTCTCTTCATGGAATGACACGATTTTGAATGACTTGGTCAAGGAGGTTGCAAGTCTTGAGGAAGAAGTTCGACAAAGTTACTCAGTACCTGTGAAGTTCAGTAAAGATGAGTTTGTTAAAGTTTTGTTAGTCGATGGTTGCTTTATCATTGAGCTCTTCCGTAAAAGTGGAGCTGACATACCAATATTGCAAGATGATCCGATACACGGTAGGGCTTGTATGCTTGAGGTAGTAGGCTGTGACATATTTTTGTTGGAAAATCAAATACCTTGGGTGGTGCTCGATCGTTTGTTTAGCAAGACCACTCTTCCTAATGGGAAGCAATCTCTGATTGAATTAGCTCTCAGTAGCTTTGAATTCTTGGGGATAGGAGCACTGGAAATGGAAACAAGTTTGATTCCAACTACTTTGGCCCAAAGCAAACATATACTTGAGTTGTTAAGAAATCTGCTAGTTGGATCGACTATGCCTGAGGGTGGGGAGCTAGATGCAACCTCATGGGAACTAATTCCCAGCGCCTCAAGTCTGATAGAAGCTGGAGTGAAGTTCAAAAAGGGTAATCCATCTAATGGCATATTGGATATCAAGTTCAGTGATGGGGTACTTGAAATACCGACTTTGGTTATACATGAGGCAACAGAAGCTTTATTCAGAAATTTAATCAGCTTTGAACAGTGTTACCATTGGTGTGATTCTAGGATCACTTCTTATGCCATGCTCCTAGATATACTCATCAACACTCCGAAAGACATGGATATACTATGTAAAAATGGTATTATTCAGAATTGGTTGAATCCAGAAGAGGCGACCGAGATCTTCAACAACCTTTACAATAATACAAATGTCGACACTTTCTACTATCTCGAGCTTTGTCAGGAAGTGAACAAGTATTGCAGACGTCGCTGGCCGAGATGGAGAGCAACATACGTGCGAAATCATTTCAGCAGTCCATGGGCAATTGCTTCCCAAGCATTTGCAGCTGTCATATTTGTACTCGCCTTACTGCAAACAGTGTTTGGCATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

507

Amino Acids

57.09

Weight (kDa)

5.83

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 114 - 495 2.1e-100 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1390
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccI GTMKAC 1 cut(s) 1335
AccII CGCG 1 cut(s) 472
AclWI GGATC 3 cut(s) 680, 955, 1196
AcoI YGGCCR 2 cut(s) 438, 1394
AcsI RAATTY 2 cut(s) 845, 1144
AcuI CTGAAG 2 cut(s) 349, 482
AcyI GRCGYC 1 cut(s) 1387
AfaI GTAC 4 cut(s) 118, 576, 1092, 1482
AfiI CCNNNNNNNGG 3 cut(s) 695, 965, 1058
AgsI TTSAA 9 cut(s) 48, 517, 824, 845, 1039, 1097, 1160, 1282, 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AloI GAACNNNNNNTCC 2 cut(s) 1061, 1093
Alw21I GWGCWC 3 cut(s) 645, 771, 865
Alw26I GTCTC 2 cut(s) 468, 496
AlwI GGATC 3 cut(s) 680, 955, 1196
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 1352
AoxI GGCC 3 cut(s) 438, 900, 1394
ApeKI GCWGC 4 cut(s) 161, 209, 263, 1466
ApoI RAATTY 2 cut(s) 845, 1144
Asp700I GAANNNNTTC 2 cut(s) 555, 996
AspLEI GCGC 2 cut(s) 472, 1007
AspS9I GGNCC 1 cut(s) 901
AsuHPI GGTGA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 1352
AxyI CCTNAGG 1 cut(s) 960
BanII GRGCYC 1 cut(s) 645
BauI CACGAG 1 cut(s) 135
Bbv12I GWGCWC 3 cut(s) 645, 771, 865
BbvI GCAGC 4 cut(s) 173, 196, 275, 1478
BccI CCATC 4 cut(s) 617, 1060, 1079, 1395
BcoDI GTCTC 2 cut(s) 468, 496
BfaI CTAG 4 cut(s) 941, 974, 1185, 1211
BfoI RGCGCY 1 cut(s) 1008
BglII AGATCT 2 cut(s) 182, 1302
BisI GCNGC 4 cut(s) 162, 210, 264, 1467
BlsI GCNGC 4 cut(s) 163, 211, 265, 1468
BmeT110I CYCGRG 1 cut(s) 1352
BmgT120I GGNCC 1 cut(s) 901
BmsI GCATC 3 cut(s) 13, 348, 967
BpmI CTGGAG 1 cut(s) 1047
BpuEI CTTGAG 5 cut(s) 301, 566, 734, 941, 994
BsaAI YACGTR 1 cut(s) 1417
BsaHI GRCGYC 1 cut(s) 1387
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 4 cut(s) 120, 421, 758, 1439
Bsc4I CCNNNNNNNGG 3 cut(s) 695, 965, 1058
Bse1I ACTGG 1 cut(s) 870
Bse21I CCTNAGG 1 cut(s) 960
Bse3DI GCAATG 1 cut(s) 413
BseDI CCNNGG 4 cut(s) 120, 421, 758, 1439
BseLI CCNNNNNNNGG 3 cut(s) 695, 965, 1058
BseMI GCAATG 1 cut(s) 413
BseMII CTCAG 4 cut(s) 42, 585, 847, 951
BseNI ACTGG 1 cut(s) 870
BseXI GCAGC 4 cut(s) 173, 196, 275, 1478
BseYI CCCAGC 1 cut(s) 1001
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 472
Bsh1285I CGRYCG 2 cut(s) 775, 1299
BshFI GGCC 3 cut(s) 440, 902, 1396
BsiEI CGRYCG 2 cut(s) 775, 1299
BsiHKAI GWGCWC 3 cut(s) 645, 771, 865
BsiHKCI CYCGRG 1 cut(s) 1352
BslI CCNNNNNNNGG 3 cut(s) 695, 965, 1058
BsmAI GTCTC 2 cut(s) 468, 496
BsmI GAATGC 1 cut(s) 398
BsnI GGCC 3 cut(s) 440, 902, 1396
Bso31I GGTCTC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 1352
Bsp1286I GDGCHC 3 cut(s) 645, 771, 865
Bsp143I GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
Bsp19I CCATGG 3 cut(s) 120, 421, 1439
BspANI GGCC 3 cut(s) 440, 902, 1396
BspCNI CTCAG 4 cut(s) 41, 584, 846, 952
BspFNI CGCG 1 cut(s) 472
BspPI GGATC 3 cut(s) 680, 955, 1196
BspQI GCTCTTC 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 413
BsrI ACTGG 1 cut(s) 870
BssECI CCNNGG 4 cut(s) 120, 421, 758, 1439
BssMI GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
BssNI GRCGYC 1 cut(s) 1387
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 4 cut(s) 120, 421, 758, 1439
Bst2BI CACGAG 1 cut(s) 135
Bst4CI ACNGT 3 cut(s) 698, 1164, 1501
Bst6I CTCTTC 5 cut(s) 368, 499, 650, 801, 1284
BstACI GRCGYC 1 cut(s) 1387
BstBAI YACGTR 1 cut(s) 1417
BstC8I GCNNGC 1 cut(s) 1394
BstDEI CTNAG 4 cut(s) 28, 571, 833, 960
BstDSI CCRYGG 3 cut(s) 120, 421, 1439
BstFNI CGCG 1 cut(s) 472
BstH2I RGCGCY 1 cut(s) 1008
BstHHI GCGC 2 cut(s) 472, 1007
BstKTI GATC 8 cut(s) 80, 101, 185, 688, 775, 950, 1191, 1305
BstMAI GTCTC 2 cut(s) 468, 496
BstMBI GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
BstMCI CGRYCG 2 cut(s) 775, 1299
BstMWI GCNNNNNNNGC 4 cut(s) 1130, 1389, 1457, 1466
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 4 cut(s) 173, 196, 275, 1478
BstX2I RGATCY 2 cut(s) 182, 1302
BstYI RGATCY 2 cut(s) 182, 1302
Bsu36I CCTNAGG 1 cut(s) 960
BsuRI GGCC 3 cut(s) 440, 902, 1396
BtgI CCRYGG 3 cut(s) 120, 421, 1439
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 5 cut(s) 212, 863, 1087, 1169, 1506
Cac8I GCNNGC 1 cut(s) 1394
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 2 cut(s) 472, 1007
Cfr13I GGNCC 1 cut(s) 901
Csp6I GTAC 4 cut(s) 117, 575, 1091, 1481
CviQI GTAC 4 cut(s) 117, 575, 1091, 1481
DdeI CTNAG 4 cut(s) 28, 571, 833, 960
DpnI GATC 8 cut(s) 79, 100, 184, 687, 774, 949, 1190, 1304
DpnII GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
EaeI YGGCCR 2 cut(s) 438, 1394
Eam1104I CTCTTC 5 cut(s) 368, 499, 650, 801, 1284
EarI CTCTTC 5 cut(s) 368, 499, 650, 801, 1284
Ecl136II GAGCTC 1 cut(s) 643
Eco130I CCWWGG 4 cut(s) 120, 421, 758, 1439
Eco24I GRGCYC 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 468
Eco32I GATATC 1 cut(s) 1072
Eco53kI GAGCTC 1 cut(s) 643
Eco57I CTGAAG 2 cut(s) 349, 482
Eco81I CCTNAGG 1 cut(s) 960
Eco88I CYCGRG 1 cut(s) 1352
EcoICRI GAGCTC 1 cut(s) 643
EcoRI GAATTC 1 cut(s) 845
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 4 cut(s) 120, 421, 758, 1439
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 4 cut(s) 120, 421, 758, 1439
FblI GTMKAC 1 cut(s) 1335
Fnu4HI GCNGC 4 cut(s) 162, 210, 264, 1467
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 4 cut(s) 162, 210, 264, 1467
FspBI CTAG 4 cut(s) 941, 974, 1185, 1211
GlaI GCGC 2 cut(s) 471, 1006
GluI GCNGC 4 cut(s) 162, 210, 264, 1467
GsaI CCCAGC 1 cut(s) 1005
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 1008
HaeIII GGCC 3 cut(s) 440, 902, 1396
HhaI GCGC 2 cut(s) 472, 1007
Hin1I GRCGYC 1 cut(s) 1387
Hin6I GCGC 2 cut(s) 470, 1005
HinP1I GCGC 2 cut(s) 470, 1005
HincII GTYRAC 2 cut(s) 327, 1336
HindII GTYRAC 2 cut(s) 327, 1336
HindIII AAGCTT 3 cut(s) 53, 381, 1131
HinfI GANTC 6 cut(s) 236, 305, 489, 886, 1181, 1282
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 3 cut(s) 327, 1336, 1372
Hpy188I TCNGA 8 cut(s) 368, 410, 690, 819, 1017, 1142, 1233, 1272
Hpy188III TCNNGA 7 cut(s) 92, 223, 545, 1286, 1352, 1364, 1513
Hpy8I GTNNAC 3 cut(s) 327, 1336, 1372
Hpy99I CGWCG 1 cut(s) 1392
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 3 cut(s) 698, 1164, 1501
HpyCH4IV ACGT 2 cut(s) 1387, 1416
HpyF10VI GCNNNNNNNGC 4 cut(s) 1130, 1389, 1457, 1466
HpyF3I CTNAG 4 cut(s) 28, 571, 833, 960
HpySE526I ACGT 2 cut(s) 1387, 1416
Hsp92I GRCGYC 1 cut(s) 1387
HspAI GCGC 2 cut(s) 470, 1005
Kzo9I GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 4 cut(s) 659, 860, 970, 1212
Lsp1109I GCAGC 4 cut(s) 173, 196, 275, 1478
LweI GCATC 3 cut(s) 13, 348, 967
MaeI CTAG 4 cut(s) 941, 974, 1185, 1211
MaeII ACGT 2 cut(s) 1387, 1416
MaeIII GTNAC 4 cut(s) 346, 566, 728, 1166
MalI GATC 8 cut(s) 79, 100, 184, 687, 774, 949, 1190, 1304
MboI GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
MboII GAAGA 9 cut(s) 177, 231, 355, 486, 563, 637, 788, 1297, 1301
MfeI CAATTG 1 cut(s) 1446
MflI RGATCY 2 cut(s) 182, 1302
MhlI GDGCHC 3 cut(s) 645, 771, 865
MluCI AATT 8 cut(s) 150, 356, 824, 845, 996, 1144, 1273, 1446
MlyI GAGTC 2 cut(s) 314, 498
MmeI TCCRAC 3 cut(s) 723, 914, 925
MnlI CCTC 9 cut(s) 488, 526, 541, 709, 955, 994, 1018, 1114, 1285
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 555, 996
MseI TTAA 3 cut(s) 606, 929, 1148
MslI CAYNNNNRTG 2 cut(s) 344, 1176
MspA1I CMGCKG 3 cut(s) 164, 266, 1469
MunI CAATTG 1 cut(s) 1446
Mva1269I GAATGC 1 cut(s) 398
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 4 cut(s) 1130, 1389, 1457, 1466
NcoI CCATGG 3 cut(s) 120, 421, 1439
NdeII GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
NmeAIII GCCGAG 1 cut(s) 1422
NmuCI GTSAC 2 cut(s) 346, 728
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 1352
PciSI GCTCTTC 1 cut(s) 650
PctI GAATGC 1 cut(s) 398
PdmI GAANNNNTTC 2 cut(s) 555, 996
PfeI GAWTC 4 cut(s) 236, 886, 1181, 1282
PflFI GACNNNGTC 1 cut(s) 524
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 4 cut(s) 163, 211, 265, 1468
Ple19I CGATCG 1 cut(s) 775
PleI GAGTC 2 cut(s) 313, 497
PpsI GAGTC 2 cut(s) 313, 497
Ppu21I YACGTR 1 cut(s) 1417
Psp124BI GAGCTC 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 1001
PspPI GGNCC 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 182, 1302
PsyI GACNNNGTC 1 cut(s) 524
PvuI CGATCG 1 cut(s) 775
PvuII CAGCTG 3 cut(s) 164, 266, 1469
RsaI GTAC 4 cut(s) 118, 576, 1092, 1482
RsaNI GTAC 4 cut(s) 117, 575, 1091, 1481
RseI CAYNNNNRTG 2 cut(s) 344, 1176
SacI GAGCTC 1 cut(s) 645
SalI GTCGAC 1 cut(s) 1334
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 3 cut(s) 606, 929, 1148
SatI GCNGC 4 cut(s) 162, 210, 264, 1467
Sau3AI GATC 8 cut(s) 77, 98, 182, 685, 772, 947, 1188, 1302
Sau96I GGNCC 1 cut(s) 901
SchI GAGTC 2 cut(s) 314, 498
SduI GDGCHC 3 cut(s) 645, 771, 865
SfaNI GCATC 3 cut(s) 13, 348, 967
Sfr274I CTCGAG 1 cut(s) 1352
SlaI CTCGAG 1 cut(s) 1352
SmiMI CAYNNNNRTG 2 cut(s) 344, 1176
SmlI CTYRAG 6 cut(s) 280, 545, 713, 920, 1009, 1352
SmoI CTYRAG 6 cut(s) 280, 545, 713, 920, 1009, 1352
Sse9I AATT 8 cut(s) 150, 356, 824, 845, 996, 1144, 1273, 1446
SspI AATATT 1 cut(s) 675
SspMI CTAG 4 cut(s) 941, 974, 1185, 1211
SstI GAGCTC 1 cut(s) 645
StyI CCWWGG 4 cut(s) 120, 421, 758, 1439
TaaI ACNGT 3 cut(s) 698, 1164, 1501
TaiI ACGT 2 cut(s) 1390, 1419
TaqII GACCGA 1 cut(s) 1313
TasI AATT 8 cut(s) 150, 356, 824, 845, 996, 1144, 1273, 1446
TatI WGTACW 1 cut(s) 1480
TfiI GAWTC 4 cut(s) 236, 886, 1181, 1282
Tru1I TTAA 3 cut(s) 606, 929, 1148
Tru9I TTAA 3 cut(s) 606, 929, 1148
TscAI CASTG 5 cut(s) 219, 870, 1087, 1169, 1506
TseFI GTSAC 2 cut(s) 346, 728
TseI GCWGC 4 cut(s) 161, 209, 263, 1466
Tsp45I GTSAC 2 cut(s) 346, 728
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 5 cut(s) 219, 870, 1087, 1169, 1506
Tth111I GACNNNGTC 1 cut(s) 524
Van91I CCANNNNNTGG 1 cut(s) 1058
XapI RAATTY 2 cut(s) 845, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 1352
XmiI GTMKAC 1 cut(s) 1335
XmnI GAANNNNTTC 2 cut(s) 555, 996
XspI CTAG 4 cut(s) 941, 974, 1185, 1211
ZraI GACGTC 1 cut(s) 1388
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.