Rh6BG172600

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011564
Physical Location & Seq
Forward (+)
28480571 .. 28483887
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG172600.1

Sequence Viewer

Length: 1350 bp
ATGGCAATTAAGGAGAGGAAAGATCAAGAATTGAAGAAAAAGCTAGCAGACACCAGGAACAATATCATGGAGAGTTTTGGAGGAGATGACATTGCAACTATAGCAAGTGATTTGATATCTTCCATGAGCAGCATTGTGCCTGAAAATTCTTTACTGTCGACCAAATGTTGCATCTTTGCAACCCCAAAATTACTCTTGAAAGATAATGAAAAATCTTATTTACCCACGGCATTCGCCATTGGGCCAGTGCACCATGGCAAACCAGACTTGCAGGAGGCAGAGCAAATTAAACTGAAGTATACACGAGACCTCATTGTTCGAGTCTCACAATGGAATGACACGATTGTGAAGGACTTAGTCGAAGAGATTGCAAGTCTTGAGGAACAAGCCCGCCAATACTACGCTGTGCCAGTGAAATTCAATAAAGCAAAGTTTGTAAAACTGTTGTTGGTTGATGGTTGCTTTATCATCGAGCTGTTCCGTAAAAAGCTAGGTGATTCACCTATTTATGAGGCTGACCCAGTATATGGTAGGCCTTTCTTACGTGAGGTATTAGGCACTGACTTGTTATTACTAGAAAACCAAATACCTTGGATGGTTCTCGATCGATTGCTTAGCAGCACAACTCGTACTGGTGCAAGTCCCCTGACTCGAGTAACCTTATTCTTCTTTAGTTTCTTGGGAACAGGAGGGTTAGATCTACAAACGGAAAATAGTTTCATGCCCAGCACTTTAGATCAAAGCAAGCACGTACTTGAGCTGTTAAGAAACTCATTAGTTTGGTCAACTATTCCAGAAGGAGGCGTGCTTGATACATGGGAGCTATTTCAAAGTGCCTCGAGTCTGATGGAAGCTGGTGTTAAATTCAAGAAGGGTAGTACATCGAATGGCTTATTGGATATCAAATTCAGTAATGGGGTACTTGAAATACCACCTTTGGTTATTGTCGAACCGACAAAAGCTTTCTTCCGAAGCTTGGTTAGCTATGAGCAGTGTTACCATTGGTGCCATCCTAAGACCACTTCTTATGCCATGTTTTTAGATCTTCTCATCAACACTTCAAAAGATATGGATATACTCTGCAAAAACGGAATCATTCAGAATTGGTTGAATCCAGAAGATGCGACCCAGATCTTCAACAACCTTTACAATGATGCAAATGTCAGCCACTTCTATTATCTTGAACTTTGCCGGGAAGTGAACAAGTATTGCAAACGTCGTTGGCCAAGATGGCGAGCAATGTATGTGCGAAATCATTTCAGCAGTCCATGGGCAATCGCTTCTCAAGTTTTGGCAGCTATCATATTTATTTTCACATTGTTGCAAACAGTGTTTAGCATCAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

449

Amino Acids

51.18

Weight (kDa)

7.04

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 58 - 437 6.7e-97 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1005
AccB7I CCANNNNNTGG 1 cut(s) 527
AccI GTMKAC 2 cut(s) 158, 299
AciI CCGC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 1223
AcsI RAATTY 4 cut(s) 145, 416, 863, 905
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 4 cut(s) 631, 753, 880, 921
AfiI CCNNNNNNNGG 3 cut(s) 527, 800, 976
AjnI CCWGG 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AleI CACNNNNGTG 1 cut(s) 344
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 300, 328
Alw44I GTGCAC 1 cut(s) 248
Ama87I CYCGRG 2 cut(s) 651, 838
AoxI GGCC 3 cut(s) 242, 533, 1223
ApaLI GTGCAC 1 cut(s) 248
ApeKI GCWGC 3 cut(s) 129, 618, 1295
ApoI RAATTY 4 cut(s) 145, 416, 863, 905
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 1193
AsuHPI GGTGA 2 cut(s) 492, 506
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 651, 838
BaeGI GKGCMC 1 cut(s) 252
BalI TGGCCA 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 1005
BauI CACGAG 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 141, 630, 1307
BccI CCATC 5 cut(s) 449, 589, 841, 1017, 1224
BceAI ACGGC 1 cut(s) 243
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 55
BcnI CCSGG 1 cut(s) 1193
BcoDI GTCTC 2 cut(s) 300, 328
BfaI CTAG 3 cut(s) 44, 491, 575
BfmI CTRYAG 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 1231
BglII AGATCT 3 cut(s) 697, 1042, 1131
BisI GCNGC 3 cut(s) 130, 619, 1296
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 3 cut(s) 131, 620, 1297
Bme1390I CCNGG 2 cut(s) 55, 1193
BmeT110I CYCGRG 2 cut(s) 651, 838
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 1007
BmrFI CCNGG 2 cut(s) 55, 1193
BmrI ACTGGG 1 cut(s) 515
BmsI GCATC 3 cut(s) 180, 1111, 1144
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 614
BpuEI CTTGAG 3 cut(s) 398, 776, 1269
BpuMI CCSGG 1 cut(s) 1193
Bsa29I ATCGAT 1 cut(s) 607
BsaAI YACGTR 2 cut(s) 545, 751
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 4 cut(s) 225, 253, 590, 1268
Bsc4I CCNNNNNNNGG 3 cut(s) 527, 800, 976
Bse1I ACTGG 4 cut(s) 245, 410, 521, 637
Bse3DI GCAATG 2 cut(s) 90, 1245
BseBI CCWGG 1 cut(s) 55
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 4 cut(s) 225, 253, 590, 1268
BseGI GGATG 2 cut(s) 600, 1009
BseLI CCNNNNNNNGG 3 cut(s) 527, 800, 976
BseMI GCAATG 2 cut(s) 90, 1245
BseNI ACTGG 4 cut(s) 245, 410, 521, 637
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 3 cut(s) 141, 630, 1307
BseYI CCCAGC 1 cut(s) 725
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 3 cut(s) 244, 535, 1225
BshNI GGYRCC 1 cut(s) 1005
BshVI ATCGAT 1 cut(s) 607
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 651, 838
BsiSI CCGG 1 cut(s) 1192
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 3 cut(s) 527, 800, 976
BsmAI GTCTC 2 cut(s) 300, 328
BsmFI GGGAC 1 cut(s) 627
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 3 cut(s) 244, 535, 1225
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 2 cut(s) 651, 838
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
Bsp1720I GCTNAGC 1 cut(s) 614
Bsp19I CCATGG 2 cut(s) 253, 1268
BspACI CCGC 1 cut(s) 391
BspANI GGCC 3 cut(s) 244, 535, 1225
BspDI ATCGAT 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 1007
BspOI GCTAGC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 1005
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 2 cut(s) 90, 1245
BsrI ACTGG 4 cut(s) 245, 410, 521, 637
BssECI CCNNGG 4 cut(s) 225, 253, 590, 1268
BssMI GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
BssNAI GTATAC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 253, 590, 1268
Bst1107I GTATAC 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 3 cut(s) 156, 444, 1330
Bst6I CTCTTC 1 cut(s) 357
BstBAI YACGTR 2 cut(s) 545, 751
BstC8I GCNNGC 5 cut(s) 45, 391, 746, 806, 1236
BstDEI CTNAG 3 cut(s) 355, 614, 1014
BstDSI CCRYGG 3 cut(s) 225, 253, 1268
BstF5I GGATG 2 cut(s) 600, 1009
BstKTI GATC 6 cut(s) 25, 607, 700, 739, 1045, 1134
BstMAI GTCTC 2 cut(s) 300, 328
BstMBI GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 3 cut(s) 101, 981, 1231
BstNI CCWGG 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 53, 1191
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 3 cut(s) 141, 630, 1307
BstX2I RGATCY 3 cut(s) 697, 1042, 1131
BstYI RGATCY 3 cut(s) 697, 1042, 1131
BstZ17I GTATAC 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 607
BsuRI GGCC 3 cut(s) 244, 535, 1225
BsuTUI ATCGAT 1 cut(s) 607
BtgI CCRYGG 3 cut(s) 225, 253, 1268
BtsCI GGATG 2 cut(s) 600, 1009
BtsI GCAGTG 1 cut(s) 998
BtsIMutI CAGTG 5 cut(s) 252, 417, 558, 998, 1335
Cac8I GCNNGC 5 cut(s) 45, 391, 746, 806, 1236
Cfr13I GGNCC 1 cut(s) 242
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 4 cut(s) 630, 752, 879, 920
CviAII CATG 7 cut(s) 67, 124, 254, 721, 816, 1033, 1269
CviQI GTAC 4 cut(s) 630, 752, 879, 920
DdeI CTNAG 3 cut(s) 355, 614, 1014
DpnI GATC 6 cut(s) 24, 606, 699, 738, 1044, 1133
DpnII GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
EaeI YGGCCR 1 cut(s) 1223
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco130I CCWWGG 3 cut(s) 253, 590, 1268
Eco147I AGGCCT 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 2 cut(s) 117, 901
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 2 cut(s) 651, 838
EcoRII CCWGG 1 cut(s) 53
EcoRV GATATC 2 cut(s) 117, 901
EcoT14I CCWWGG 3 cut(s) 253, 590, 1268
ErhI CCWWGG 3 cut(s) 253, 590, 1268
FaeI CATG 7 cut(s) 70, 127, 257, 724, 819, 1036, 1272
FaqI GGGAC 1 cut(s) 627
FatI CATG 7 cut(s) 66, 123, 253, 720, 815, 1032, 1268
FauI CCCGC 1 cut(s) 398
FblI GTMKAC 2 cut(s) 158, 299
Fnu4HI GCNGC 3 cut(s) 130, 619, 1296
FokI GGATG 2 cut(s) 607, 996
Fsp4HI GCNGC 3 cut(s) 130, 619, 1296
FspBI CTAG 3 cut(s) 44, 491, 575
GluI GCNGC 3 cut(s) 130, 619, 1296
GsaI CCCAGC 1 cut(s) 729
HaeIII GGCC 3 cut(s) 244, 535, 1225
HapII CCGG 1 cut(s) 1192
Hin1II CATG 7 cut(s) 70, 127, 257, 724, 819, 1036, 1272
HincII GTYRAC 2 cut(s) 159, 786
HindII GTYRAC 2 cut(s) 159, 786
HindIII AAGCTT 2 cut(s) 960, 973
HinfI GANTC 6 cut(s) 321, 497, 649, 841, 1092, 1111
HpaII CCGG 1 cut(s) 1192
HphI GGTGA 2 cut(s) 492, 506
Hpy166II GTNNAC 5 cut(s) 159, 250, 300, 786, 1201
Hpy188I TCNGA 3 cut(s) 846, 971, 1101
Hpy188III TCNNGA 9 cut(s) 26, 196, 377, 602, 794, 868, 1115, 1181, 1342
Hpy8I GTNNAC 5 cut(s) 159, 250, 300, 786, 1201
Hpy99I CGWCG 1 cut(s) 1221
HpyAV CCTTC 3 cut(s) 343, 791, 865
HpyCH4III ACNGT 3 cut(s) 156, 444, 1330
HpyCH4IV ACGT 3 cut(s) 544, 750, 1216
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 981, 1231
HpyF3I CTNAG 3 cut(s) 355, 614, 1014
HpySE526I ACGT 3 cut(s) 544, 750, 1216
Hsp92II CATG 7 cut(s) 70, 127, 257, 724, 819, 1036, 1272
Kzo9I GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 3 cut(s) 141, 630, 1307
LweI GCATC 3 cut(s) 180, 1111, 1144
MaeI CTAG 3 cut(s) 44, 491, 575
MaeII ACGT 3 cut(s) 544, 750, 1216
MaeIII GTNAC 2 cut(s) 655, 995
MalI GATC 6 cut(s) 24, 606, 699, 738, 1044, 1133
MboI GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
MboII GAAGA 8 cut(s) 46, 111, 374, 658, 958, 1037, 1126, 1130
MflI RGATCY 3 cut(s) 697, 1042, 1131
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 1225
MluCI AATT 9 cut(s) 6, 29, 145, 188, 285, 416, 863, 905, 1102
MluNI TGGCCA 1 cut(s) 1225
MlyI GAGTC 3 cut(s) 330, 643, 850
Mox20I TGGCCA 1 cut(s) 1225
MscI TGGCCA 1 cut(s) 1225
MseI TTAA 5 cut(s) 9, 288, 764, 861, 1348
MslI CAYNNNNRTG 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 1225
MspI CCGG 1 cut(s) 1192
MspR9I CCNGG 2 cut(s) 55, 1193
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 3 cut(s) 101, 981, 1231
NciI CCSGG 1 cut(s) 1193
NcoI CCATGG 2 cut(s) 253, 1268
NdeII GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 7 cut(s) 70, 127, 257, 724, 819, 1036, 1272
NlaIV GGNNCC 1 cut(s) 1007
OliI CACNNNNGTG 1 cut(s) 344
PaeR7I CTCGAG 2 cut(s) 651, 838
PceI AGGCCT 1 cut(s) 535
PctI GAATGC 1 cut(s) 230
PfeI GAWTC 3 cut(s) 497, 1092, 1111
PflFI GACNNNGTC 1 cut(s) 356
PflMI CCANNNNNTGG 1 cut(s) 527
PkrI GCNGC 3 cut(s) 131, 620, 1297
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 3 cut(s) 329, 643, 849
PpsI GAGTC 3 cut(s) 329, 643, 849
Ppu21I YACGTR 2 cut(s) 545, 751
Psp6I CCWGG 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 725
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 1007
PspPI GGNCC 1 cut(s) 242
PspXI VCTCGAGB 2 cut(s) 651, 838
PsuI RGATCY 3 cut(s) 697, 1042, 1131
PsyI GACNNNGTC 1 cut(s) 356
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 4 cut(s) 631, 753, 880, 921
RsaNI GTAC 4 cut(s) 630, 752, 879, 920
RseI CAYNNNNRTG 1 cut(s) 344
SalI GTCGAC 1 cut(s) 157
SaqAI TTAA 5 cut(s) 9, 288, 764, 861, 1348
SatI GCNGC 3 cut(s) 130, 619, 1296
Sau3AI GATC 6 cut(s) 22, 604, 697, 736, 1042, 1131
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 3 cut(s) 330, 643, 850
ScrFI CCNGG 2 cut(s) 55, 1193
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 3 cut(s) 180, 1111, 1144
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 2 cut(s) 651, 838
SlaI CTCGAG 2 cut(s) 651, 838
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 5 cut(s) 377, 651, 755, 838, 1284
SmoI CTYRAG 5 cut(s) 377, 651, 755, 838, 1284
Sse9I AATT 9 cut(s) 6, 29, 145, 188, 285, 416, 863, 905, 1102
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 1 cut(s) 391
SspMI CTAG 3 cut(s) 44, 491, 575
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 2 cut(s) 53, 1191
StyI CCWWGG 3 cut(s) 253, 590, 1268
TaaI ACNGT 3 cut(s) 156, 444, 1330
TaiI ACGT 3 cut(s) 547, 753, 1219
TasI AATT 9 cut(s) 6, 29, 145, 188, 285, 416, 863, 905, 1102
TatI WGTACW 1 cut(s) 878
TfiI GAWTC 3 cut(s) 497, 1092, 1111
Tru1I TTAA 5 cut(s) 9, 288, 764, 861, 1348
Tru9I TTAA 5 cut(s) 9, 288, 764, 861, 1348
TscAI CASTG 5 cut(s) 252, 417, 565, 998, 1335
TseI GCWGC 3 cut(s) 129, 618, 1295
TspDTI ATGAA 2 cut(s) 222, 709
TspGWI ACGGA 3 cut(s) 470, 722, 1104
TspRI CASTG 5 cut(s) 252, 417, 565, 998, 1335
Tth111I GACNNNGTC 1 cut(s) 356
Van91I CCANNNNNTGG 1 cut(s) 527
VneI GTGCAC 1 cut(s) 248
XapI RAATTY 4 cut(s) 145, 416, 863, 905
XhoI CTCGAG 2 cut(s) 651, 838
XmiI GTMKAC 2 cut(s) 158, 299
XspI CTAG 3 cut(s) 44, 491, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.