Rroxscaffold_3G00247590

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011564
Physical Location & Seq
Reverse (-)
40907636 .. 40933656
26021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247590.1

Sequence Viewer

Length: 1299 bp
ATGCAAGGTTTTGGAAGAGATGGCACCACAATTGATACTGCCAATTTGCAAAGAGACTGGCTATCTTCCATGAGCAATATTGTGGTTGAAAATTCTTTATTGTCGACCAAATGCTGCATCTTCGTAACTCCAAAACTACTCTTCAGAAACAATGAAAAAGCATATGTCCCCACAGCATTCTCAATCGGCCCTTTGCACCATGGCAAACCAGAATTTAAAGAGGCGGAGCAAATTAAACTGAAATATACGCGAGACCTCATCTGTCGAGTCCAGAATTGGAACCCAACGATTGTGCGGGACTTGGTCGAAGATATTGCAGGTCTCGAGAAAGAAGCCCGCGAATGTTACTCTGTACCGGTGGAACCCAGAAGGGATGAGTTTATTAAAATTTTGTTAGTTGATGGTTGCTTTCTCATCGAGCTATTCCGTAATCGTGCTGGGCCTTTTTACAATGAGGGTGACCCCGTGTTCAGTAGGCCTTTCTTACGTGGGGTATTAGACACTGACTTGTTATTACTAGAAAACCAAATACCTTGGATAGTGCTCGATCGATTGTTTAGTCGGACCACTCCTCCTCCGGCCACTCCTCCTCGAACAGATCTCCTGACTCAGCTAGCCTGGTACTATATTAGTTGCATAACGACTGAACCTCATCCGAAAAAGCAAAATATACCCAGCACTTTGGGTCCAAGAGAGCACCTACTTGAGCTATTGAGAAGTTCATTTATTGGATCAACTATGACACGAGGAGGTAACCTTAATGAATGGGAATTAATTCCGAGTGTCTCAAGTTTAATAGAAGCTGGAGTGAAATTCCAGAAGCCTAGTACAGATTGTGGCATTTTGGATATCAAATTTAGTAATGGGATACTCGAAATACCACCTTTGACCATTCATGAGTCAACTGAATCTTTATTCCGAAACATGATAAGCTTTGAACAGTGTTCCAACTGGTGTGAGCCTAGGATCACTTGTTATGCCATGCTTTTAGATAGACTTATCAACACTCCGAAGGACATGGATATACTTGTTCAAAAAGGAATTATTCGAAACTGGTTGAATCCAGAAGAGGCCACCGATATCTTCAACAAGCTTTATTATGATGCATATGTCACCGAGTTCTATTACCTCGAGCTTTGCAAGGAAGTGAACAAGTATTGCAGACGTCGTTGGCCAAGATGGCGAGCAATATATGTCAGAAATCATTTTTGCAGTCCATGGGCAATCGCTTCACAAGTGTTCGCAGCTATCATATTTGTTCTTGCATTATTGCAACCAGTTTTCGGCAACAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

432

Amino Acids

49.9

Weight (kDa)

6.99

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 40 - 421 8.6e-102 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1168
Acc36I ACCTGC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 104
AccII CGCG 2 cut(s) 250, 339
AciI CCGC 3 cut(s) 224, 295, 337
AclWI GGATC 2 cut(s) 739, 974
AcoI YGGCCR 2 cut(s) 579, 1172
AcsI RAATTY 5 cut(s) 91, 212, 387, 812, 854
AcuI CTGAAG 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 1165
AfaI GTAC 3 cut(s) 354, 623, 829
AfiI CCNNNNNNNGG 1 cut(s) 1283
AgeI ACCGGT 1 cut(s) 355
AgsI TTSAA 5 cut(s) 89, 938, 1034, 1060, 1087
AjnI CCWGG 1 cut(s) 617
AluBI AGCT 8 cut(s) 421, 613, 709, 803, 933, 1093, 1135, 1247
AluI AGCT 8 cut(s) 421, 613, 709, 803, 933, 1093, 1135, 1247
Alw21I GWGCWC 2 cut(s) 546, 699
Alw26I GTCTC 4 cut(s) 48, 246, 326, 790
AlwI GGATC 2 cut(s) 739, 974
Ama87I CYCGRG 2 cut(s) 323, 1130
AoxI GGCC 6 cut(s) 187, 440, 476, 579, 1071, 1172
ApeKI GCWGC 2 cut(s) 114, 1244
ApoI RAATTY 5 cut(s) 91, 212, 387, 812, 854
AseI ATTAAT 1 cut(s) 773
AsiGI ACCGGT 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 774
AspA2I CCTAGG 1 cut(s) 962
AspS9I GGNCC 4 cut(s) 188, 440, 564, 686
AsuHPI GGTGA 2 cut(s) 470, 1105
AsuII TTCGAA 1 cut(s) 1048
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 2 cut(s) 323, 1130
AvaII GGWCC 2 cut(s) 564, 686
AvrII CCTAGG 1 cut(s) 962
BalI TGGCCA 1 cut(s) 1174
BanI GGYRCC 1 cut(s) 23
BauI CACGAG 1 cut(s) 744
Bbv12I GWGCWC 2 cut(s) 546, 699
BbvI GCAGC 2 cut(s) 101, 1256
BccI CCATC 3 cut(s) 14, 395, 1173
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 1 cut(s) 619
BciVI GTATCC 1 cut(s) 861
BcoDI GTCTC 4 cut(s) 48, 246, 326, 790
BfaI CTAG 4 cut(s) 518, 614, 825, 963
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 861
BglI GCCNNNNNGGC 1 cut(s) 1180
BglII AGATCT 1 cut(s) 598
BisI GCNGC 2 cut(s) 115, 1245
BlnI CCTAGG 1 cut(s) 962
BlsI GCNGC 2 cut(s) 116, 1246
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 2 cut(s) 564, 686
BmeT110I CYCGRG 2 cut(s) 323, 1130
BmgT120I GGNCC 4 cut(s) 188, 440, 564, 686
BmiI GGNNCC 4 cut(s) 25, 281, 363, 687
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 2 cut(s) 126, 1093
BmtI GCTAGC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 825
Bpu14I TTCGAA 1 cut(s) 1048
BpuEI CTTGAG 2 cut(s) 725, 772
Bsa29I ATCGAT 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 1165
BsaI GGTCTC 2 cut(s) 246, 326
BsaJI CCNNGG 4 cut(s) 199, 533, 962, 1217
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 4 cut(s) 556, 559, 586, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 1283
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 4 cut(s) 62, 956, 1058, 1277
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 4 cut(s) 199, 533, 962, 1217
BseGI GGATG 2 cut(s) 379, 652
BseLI CCNNNNNNNGG 1 cut(s) 1283
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 4 cut(s) 62, 956, 1058, 1277
BseRI GAGGAG 5 cut(s) 561, 564, 576, 579, 762
BseXI GCAGC 2 cut(s) 101, 1256
BseYI CCCAGC 2 cut(s) 437, 674
Bsh1236I CGCG 2 cut(s) 250, 339
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 6 cut(s) 189, 442, 478, 581, 1073, 1174
BshNI GGYRCC 1 cut(s) 23
BshTI ACCGGT 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 550
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 546, 699
BsiHKCI CYCGRG 2 cut(s) 323, 1130
BsiSI CCGG 2 cut(s) 356, 578
BslFI GGGAC 2 cut(s) 152, 311
BslI CCNNNNNNNGG 1 cut(s) 1283
BsmAI GTCTC 4 cut(s) 48, 246, 326, 790
BsmFI GGGAC 2 cut(s) 152, 311
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 6 cut(s) 189, 442, 478, 581, 1073, 1174
Bso31I GGTCTC 2 cut(s) 246, 326
BsoBI CYCGRG 2 cut(s) 323, 1130
Bsp119I TTCGAA 1 cut(s) 1048
Bsp1286I GDGCHC 2 cut(s) 546, 699
Bsp143I GATC 4 cut(s) 547, 598, 731, 966
Bsp19I CCATGG 2 cut(s) 199, 1217
BspACI CCGC 3 cut(s) 224, 295, 337
BspANI GGCC 6 cut(s) 189, 442, 478, 581, 1073, 1174
BspCNI CTCAG 1 cut(s) 622
BspDI ATCGAT 1 cut(s) 550
BspFNI CGCG 2 cut(s) 250, 339
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 25, 281, 363, 687
BspMI ACCTGC 1 cut(s) 308
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 739, 974
BspT104I TTCGAA 1 cut(s) 1048
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 2 cut(s) 246, 326
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 4 cut(s) 62, 956, 1058, 1277
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 4 cut(s) 199, 533, 962, 1217
BssMI GATC 4 cut(s) 547, 598, 731, 966
BssNI GRCGYC 1 cut(s) 1165
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 4 cut(s) 199, 533, 962, 1217
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 1 cut(s) 942
Bst6I CTCTTC 3 cut(s) 10, 146, 1062
BstACI GRCGYC 1 cut(s) 1165
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 1048
BstC8I GCNNGC 3 cut(s) 337, 615, 1185
BstDEI CTNAG 1 cut(s) 609
BstDSI CCRYGG 2 cut(s) 199, 1217
BstEII GGTNACC 2 cut(s) 458, 752
BstF5I GGATG 2 cut(s) 379, 652
BstFNI CGCG 2 cut(s) 250, 339
BstKTI GATC 4 cut(s) 550, 601, 734, 969
BstMAI GTCTC 4 cut(s) 48, 246, 326, 790
BstMBI GATC 4 cut(s) 547, 598, 731, 966
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 1 cut(s) 1180
BstNI CCWGG 1 cut(s) 619
BstPI GGTNACC 2 cut(s) 458, 752
BstSCI CCNGG 1 cut(s) 617
BstUI CGCG 2 cut(s) 250, 339
BstV1I GCAGC 2 cut(s) 101, 1256
BstX2I RGATCY 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 682
BstYI RGATCY 1 cut(s) 598
Bsu15I ATCGAT 1 cut(s) 550
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 6 cut(s) 189, 442, 478, 581, 1073, 1174
BsuTUI ATCGAT 1 cut(s) 550
BtgI CCRYGG 2 cut(s) 199, 1217
BtsCI GGATG 2 cut(s) 379, 652
BtsIMutI CAGTG 2 cut(s) 501, 947
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 3 cut(s) 337, 615, 1185
CciI TCATGA 1 cut(s) 895
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 4 cut(s) 188, 440, 564, 686
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 3 cut(s) 353, 622, 828
CspAI ACCGGT 1 cut(s) 355
CviAII CATG 7 cut(s) 70, 200, 896, 925, 982, 1018, 1218
CviQI GTAC 3 cut(s) 353, 622, 828
DdeI CTNAG 1 cut(s) 609
DpnI GATC 4 cut(s) 549, 600, 733, 968
DpnII GATC 4 cut(s) 547, 598, 731, 966
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 579, 1172
Eam1104I CTCTTC 3 cut(s) 10, 146, 1062
EarI CTCTTC 3 cut(s) 10, 146, 1062
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 4 cut(s) 199, 533, 962, 1217
Eco147I AGGCCT 1 cut(s) 478
Eco31I GGTCTC 2 cut(s) 246, 326
Eco32I GATATC 2 cut(s) 850, 1081
Eco47I GGWCC 2 cut(s) 564, 686
Eco57I CTGAAG 1 cut(s) 127
Eco88I CYCGRG 2 cut(s) 323, 1130
Eco91I GGTNACC 2 cut(s) 458, 752
EcoO65I GGTNACC 2 cut(s) 458, 752
EcoRII CCWGG 1 cut(s) 617
EcoRV GATATC 2 cut(s) 850, 1081
EcoT14I CCWWGG 4 cut(s) 199, 533, 962, 1217
EcoT22I ATGCAT 1 cut(s) 1108
ErhI CCWWGG 4 cut(s) 199, 533, 962, 1217
FaeI CATG 7 cut(s) 73, 203, 899, 928, 985, 1021, 1221
FaqI GGGAC 2 cut(s) 152, 311
FatI CATG 7 cut(s) 69, 199, 895, 924, 981, 1017, 1217
FauI CCCGC 2 cut(s) 288, 344
FauNDI CATATG 2 cut(s) 163, 1108
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 115, 1245
FokI GGATG 2 cut(s) 386, 639
Fsp4HI GCNGC 2 cut(s) 115, 1245
FspBI CTAG 4 cut(s) 518, 614, 825, 963
GluI GCNGC 2 cut(s) 115, 1245
GsaI CCCAGC 2 cut(s) 441, 678
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 6 cut(s) 189, 442, 478, 581, 1073, 1174
HapII CCGG 2 cut(s) 356, 578
Hin1I GRCGYC 1 cut(s) 1165
Hin1II CATG 7 cut(s) 73, 203, 899, 928, 985, 1021, 1221
HincII GTYRAC 2 cut(s) 105, 903
HindII GTYRAC 2 cut(s) 105, 903
HindIII AAGCTT 2 cut(s) 931, 1091
HinfI GANTC 5 cut(s) 267, 607, 899, 908, 1060
HpaII CCGG 2 cut(s) 356, 578
HphI GGTGA 2 cut(s) 470, 1105
Hpy166II GTNNAC 3 cut(s) 105, 903, 1150
Hpy188I TCNGA 7 cut(s) 146, 564, 657, 780, 920, 1011, 1199
Hpy188III TCNNGA 7 cut(s) 271, 323, 325, 604, 817, 896, 1064
Hpy8I GTNNAC 3 cut(s) 105, 903, 1150
Hpy99I CGWCG 1 cut(s) 1170
HpyAV CCTTC 2 cut(s) 363, 1006
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4IV ACGT 2 cut(s) 487, 1165
HpyF10VI GCNNNNNNNGC 1 cut(s) 1180
HpyF3I CTNAG 1 cut(s) 609
HpySE526I ACGT 2 cut(s) 487, 1165
Hsp92I GRCGYC 1 cut(s) 1165
Hsp92II CATG 7 cut(s) 73, 203, 899, 928, 985, 1021, 1221
Kzo9I GATC 4 cut(s) 547, 598, 731, 966
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 2 cut(s) 101, 1256
LweI GCATC 2 cut(s) 126, 1093
MaeI CTAG 4 cut(s) 518, 614, 825, 963
MaeII ACGT 2 cut(s) 487, 1165
MaeIII GTNAC 5 cut(s) 124, 344, 458, 752, 1111
MalI GATC 4 cut(s) 549, 600, 733, 968
MboI GATC 4 cut(s) 547, 598, 731, 966
MboII GAAGA 7 cut(s) 27, 57, 112, 133, 320, 1075, 1079
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 2 cut(s) 546, 699
MlsI TGGCCA 1 cut(s) 1174
MluNI TGGCCA 1 cut(s) 1174
MlyI GAGTC 3 cut(s) 276, 601, 908
MmeI TCCRAC 2 cut(s) 542, 972
Mox20I TGGCCA 1 cut(s) 1174
Mph1103I ATGCAT 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 774
MscI TGGCCA 1 cut(s) 1174
MseI TTAA 6 cut(s) 216, 234, 384, 759, 773, 794
Msp20I TGGCCA 1 cut(s) 1174
MspI CCGG 2 cut(s) 356, 578
MspR9I CCNGG 1 cut(s) 619
MunI CAATTG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 619
MvnI CGCG 2 cut(s) 250, 339
MwoI GCNNNNNNNGC 1 cut(s) 1180
NcoI CCATGG 2 cut(s) 199, 1217
NdeI CATATG 2 cut(s) 163, 1108
NdeII GATC 4 cut(s) 547, 598, 731, 966
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 7 cut(s) 73, 203, 899, 928, 985, 1021, 1221
NlaIV GGNNCC 4 cut(s) 25, 281, 363, 687
NmuCI GTSAC 2 cut(s) 458, 1111
NsiI ATGCAT 1 cut(s) 1108
NspV TTCGAA 1 cut(s) 1048
PaeR7I CTCGAG 2 cut(s) 323, 1130
PagI TCATGA 1 cut(s) 895
PceI AGGCCT 1 cut(s) 478
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 2 cut(s) 908, 1060
PflFI GACNNNGTC 1 cut(s) 302
PinAI ACCGGT 1 cut(s) 355
PkrI GCNGC 2 cut(s) 116, 1246
Ple19I CGATCG 1 cut(s) 550
PleI GAGTC 3 cut(s) 275, 601, 907
PpsI GAGTC 3 cut(s) 275, 601, 907
Ppu21I YACGTR 1 cut(s) 488
PshBI ATTAAT 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 617
PspEI GGTNACC 2 cut(s) 458, 752
PspFI CCCAGC 2 cut(s) 437, 674
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 4 cut(s) 25, 281, 363, 687
PspPI GGNCC 4 cut(s) 188, 440, 564, 686
PspXI VCTCGAGB 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 302
PvuI CGATCG 1 cut(s) 550
RsaI GTAC 3 cut(s) 354, 623, 829
RsaNI GTAC 3 cut(s) 353, 622, 828
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 6 cut(s) 216, 234, 384, 759, 773, 794
SatI GCNGC 2 cut(s) 115, 1245
Sau3AI GATC 4 cut(s) 547, 598, 731, 966
Sau96I GGNCC 4 cut(s) 188, 440, 564, 686
SchI GAGTC 3 cut(s) 276, 601, 908
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 546, 699
SfaNI GCATC 2 cut(s) 126, 1093
Sfr274I CTCGAG 2 cut(s) 323, 1130
SfuI TTCGAA 1 cut(s) 1048
SinI GGWCC 2 cut(s) 564, 686
SlaI CTCGAG 2 cut(s) 323, 1130
SmlI CTYRAG 4 cut(s) 323, 704, 787, 1130
SmoI CTYRAG 4 cut(s) 323, 704, 787, 1130
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 3 cut(s) 224, 295, 337
SspI AATATT 1 cut(s) 79
SspMI CTAG 4 cut(s) 518, 614, 825, 963
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 4 cut(s) 199, 533, 962, 1217
TaaI ACNGT 1 cut(s) 942
TaiI ACGT 2 cut(s) 490, 1168
TatI WGTACW 1 cut(s) 827
TfiI GAWTC 2 cut(s) 908, 1060
Tru1I TTAA 6 cut(s) 216, 234, 384, 759, 773, 794
Tru9I TTAA 6 cut(s) 216, 234, 384, 759, 773, 794
TscAI CASTG 2 cut(s) 508, 947
TseFI GTSAC 2 cut(s) 458, 1111
TseI GCWGC 2 cut(s) 114, 1244
Tsp45I GTSAC 2 cut(s) 458, 1111
TspDTI ATGAA 4 cut(s) 168, 711, 777, 884
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 508, 947
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 564, 686
VspI ATTAAT 1 cut(s) 773
XapI RAATTY 5 cut(s) 91, 212, 387, 812, 854
XhoI CTCGAG 2 cut(s) 323, 1130
XmaJI CCTAGG 1 cut(s) 962
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 4 cut(s) 518, 614, 825, 963
ZraI GACGTC 1 cut(s) 1166
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.