RchiOBHm_Chr6g0269291

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011564
Physical Location & Seq
Forward (+)
26570776 .. 26574157
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24156

Sequence Viewer

Length: 1296 bp
ATGGAGAGTTTTGGAGGAGATGACGGTGCAGATGACATTGCAACTATAGGAAGTGATTTGATATCTTCCATGAACAACATTGTGCCTGAAAATTCTTTACTGTCGACCAAATGTTGCATCTTTGCAACCCCAAAATTACTCTTGAAAGATAATGAAAAATCTTATTTACCCACGGCATTCGCCATTGGGCCAGTGCACCATGGCAAACCAGACTTGCAGGAGGCAGAGCAAATTAAACTGAAGTATACACGAGACCTCATTGCTCGAGTCTCAGAATGGAATGACACGATTGTGAAGGACTTAGTCAAAGAGATTGCAAGTCTTGAGGAACAAGCCCGCCAATACTACGCTGTGCCAGTGAAATTCAATAAAGCAAAGTTTGTAAAACTGTTGTTGGTTGATGGTTGCTTTATCATCGAGCTGTTCCGTAAAAGGCTAGGTGATTCACCTATTTATGAGGATGACCCAGTATGTGGTAGGCCTTTCTTACGTGAGGTATTAGGCACTGACTTGTTATTACTAGAAAACCAAATACCTTGGATGGTTCTCGATCGATTGTTTAGCAGCACAACCCGTACTGGTGCACTTTCCCTGACTCAAGTAACCTTATCCTTCTTTAGTTTCTTGCGAACAGGAGGGTTAGATCTACAAACGGAAAATAGTTTCATGCTCAGCACTTTAGATCAAAGCAAGCACGTACTTGAGCTGTTAAGAAACTCATTAGTTTGGTCAACTATTCCAGAAGGAGGCGAGCTTGATACATGGGAGCTAATTCCGAGTGCCTCGAGTCTGACGGAAGCTGGTGTTAAATTCAAGAAAGGTAGTACATCGAATGGCTTATTGGATATCAAATTCAGTAATGGGGTACTTGAAATACCACCTTTGGTTATTGTCGAACCGACAAAAGCTTTCTTCCGAAACTTGATTAGCTATGAGCAGTGTTACCATTGGTGCCATCCTAAGATCACTTCTTATGCCATGCTTCTAGATCTTCTCATCAACACTTCAAAAGATATGGATATACTCTGCGAAAACGGAATCATTCAGAATTGGTTGAATCCAGAAGATGCGACCCAGATCTTCAACAACCTTTACAATGATACAAATGTCAGCCACTTCTATTATCTTGAACTTTGCCGGGAAGTGAACAAGTATTGCAAACGTCGTTGGCCGAGATGGCGAGCAATGTATGTGCGAAATCATTTCAGCAGTCCGTGGGCAATCGCTTCTCAAGTTTTGGCAGCTATCATATTTATTCTCACATTGTTGCAAACAGTGTTTAGCATCAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

431

Amino Acids

48.96

Weight (kDa)

5.52

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 40 - 420 1.4e-102 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 951
AccB7I CCANNNNNTGG 1 cut(s) 473
AccI GTMKAC 2 cut(s) 104, 245
AciI CCGC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 1169
AcsI RAATTY 4 cut(s) 91, 362, 809, 851
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 4 cut(s) 577, 699, 826, 867
AfiI CCNNNNNNNGG 2 cut(s) 473, 746
AgsI TTSAA 8 cut(s) 145, 367, 814, 872, 1008, 1057, 1084, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 8 cut(s) 421, 706, 754, 769, 800, 908, 930, 1244
AluI AGCT 8 cut(s) 421, 706, 754, 769, 800, 908, 930, 1244
Alw21I GWGCWC 2 cut(s) 198, 586
Alw26I GTCTC 2 cut(s) 246, 274
Alw44I GTGCAC 2 cut(s) 194, 582
Ama87I CYCGRG 2 cut(s) 264, 784
AoxI GGCC 3 cut(s) 188, 479, 1169
ApaLI GTGCAC 2 cut(s) 194, 582
ApeKI GCWGC 2 cut(s) 564, 1241
ApoI RAATTY 4 cut(s) 91, 362, 809, 851
AspS9I GGNCC 1 cut(s) 188
AsuC2I CCSGG 1 cut(s) 1139
AsuHPI GGTGA 2 cut(s) 438, 452
AvaI CYCGRG 2 cut(s) 264, 784
BaeGI GKGCMC 2 cut(s) 198, 586
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BanI GGYRCC 1 cut(s) 951
BauI CACGAG 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 198, 586
BbvI GCAGC 2 cut(s) 576, 1253
BccI CCATC 4 cut(s) 395, 535, 963, 1170
BceAI ACGGC 1 cut(s) 189
BcnI CCSGG 1 cut(s) 1139
BcoDI GTCTC 2 cut(s) 246, 274
BfaI CTAG 3 cut(s) 437, 521, 986
BfmI CTRYAG 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 1177
BglII AGATCT 3 cut(s) 643, 988, 1077
BisI GCNGC 2 cut(s) 565, 1242
BlpI GCTNAGC 1 cut(s) 671
BlsI GCNGC 2 cut(s) 566, 1243
Bme1390I CCNGG 1 cut(s) 1139
BmeT110I CYCGRG 2 cut(s) 264, 784
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 953
BmrFI CCNGG 1 cut(s) 1139
BmrI ACTGGG 1 cut(s) 461
BmsI GCATC 2 cut(s) 126, 1057
BmuI ACTGGG 1 cut(s) 461
Bpu1102I GCTNAGC 1 cut(s) 671
BpuEI CTTGAG 4 cut(s) 344, 582, 722, 1215
BpuMI CCSGG 1 cut(s) 1139
Bsa29I ATCGAT 1 cut(s) 553
BsaAI YACGTR 2 cut(s) 491, 697
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 171, 199, 536, 1214
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 746
Bse1I ACTGG 4 cut(s) 191, 356, 467, 583
Bse3DI GCAATG 3 cut(s) 36, 258, 1191
BseCI ATCGAT 1 cut(s) 553
BseDI CCNNGG 4 cut(s) 171, 199, 536, 1214
BseGI GGATG 3 cut(s) 466, 546, 955
BseLI CCNNNNNNNGG 2 cut(s) 473, 746
BseMI GCAATG 3 cut(s) 36, 258, 1191
BseMII CTCAG 2 cut(s) 285, 685
BseNI ACTGG 4 cut(s) 191, 356, 467, 583
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 2 cut(s) 198, 586
BseXI GCAGC 2 cut(s) 576, 1253
BsgI GTGCAG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 3 cut(s) 190, 481, 1171
BshNI GGYRCC 1 cut(s) 951
BshVI ATCGAT 1 cut(s) 553
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 198, 586
BsiHKCI CYCGRG 2 cut(s) 264, 784
BsiSI CCGG 1 cut(s) 1138
BslI CCNNNNNNNGG 2 cut(s) 473, 746
BsmAI GTCTC 2 cut(s) 246, 274
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 3 cut(s) 190, 481, 1171
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 2 cut(s) 264, 784
Bsp1286I GDGCHC 2 cut(s) 198, 586
Bsp143I GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
Bsp1720I GCTNAGC 1 cut(s) 671
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 1 cut(s) 337
BspANI GGCC 3 cut(s) 190, 481, 1171
BspCNI CTCAG 2 cut(s) 284, 684
BspDI ATCGAT 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 953
BspT107I GGYRCC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 3 cut(s) 36, 258, 1191
BsrI ACTGG 4 cut(s) 191, 356, 467, 583
BssECI CCNNGG 4 cut(s) 171, 199, 536, 1214
BssMI GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
BssNAI GTATAC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 2 cut(s) 199, 536
Bst1107I GTATAC 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 249
Bst4CI ACNGT 4 cut(s) 26, 102, 390, 1276
BstBAI YACGTR 2 cut(s) 491, 697
BstC8I GCNNGC 4 cut(s) 337, 692, 752, 1182
BstDEI CTNAG 4 cut(s) 271, 301, 671, 960
BstDSI CCRYGG 3 cut(s) 171, 199, 1214
BstF5I GGATG 3 cut(s) 466, 546, 955
BstKTI GATC 6 cut(s) 553, 646, 685, 966, 991, 1080
BstMAI GTCTC 2 cut(s) 246, 274
BstMBI GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 1 cut(s) 1177
BstSCI CCNGG 1 cut(s) 1137
BstSFI CTRYAG 1 cut(s) 45
BstSLI GKGCMC 2 cut(s) 198, 586
BstV1I GCAGC 2 cut(s) 576, 1253
BstX2I RGATCY 3 cut(s) 643, 988, 1077
BstYI RGATCY 3 cut(s) 643, 988, 1077
BstZ17I GTATAC 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 553
BsuRI GGCC 3 cut(s) 190, 481, 1171
BsuTUI ATCGAT 1 cut(s) 553
BtgI CCRYGG 3 cut(s) 171, 199, 1214
BtsCI GGATG 3 cut(s) 466, 546, 955
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 5 cut(s) 198, 363, 504, 944, 1281
Cac8I GCNNGC 4 cut(s) 337, 692, 752, 1182
Cfr13I GGNCC 1 cut(s) 188
ClaI ATCGAT 1 cut(s) 553
Csp6I GTAC 4 cut(s) 576, 698, 825, 866
CviAII CATG 5 cut(s) 70, 200, 667, 762, 979
CviQI GTAC 4 cut(s) 576, 698, 825, 866
DdeI CTNAG 4 cut(s) 271, 301, 671, 960
DpnI GATC 6 cut(s) 552, 645, 684, 965, 990, 1079
DpnII GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
EaeI YGGCCR 1 cut(s) 1169
Eco130I CCWWGG 2 cut(s) 199, 536
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 246
Eco32I GATATC 2 cut(s) 63, 847
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 2 cut(s) 264, 784
EcoRV GATATC 2 cut(s) 63, 847
EcoT14I CCWWGG 2 cut(s) 199, 536
ErhI CCWWGG 2 cut(s) 199, 536
FaeI CATG 5 cut(s) 73, 203, 670, 765, 982
FatI CATG 5 cut(s) 69, 199, 666, 761, 978
FauI CCCGC 1 cut(s) 344
FblI GTMKAC 2 cut(s) 104, 245
Fnu4HI GCNGC 2 cut(s) 565, 1242
FokI GGATG 3 cut(s) 473, 553, 942
Fsp4HI GCNGC 2 cut(s) 565, 1242
FspBI CTAG 3 cut(s) 437, 521, 986
GluI GCNGC 2 cut(s) 565, 1242
HaeIII GGCC 3 cut(s) 190, 481, 1171
HapII CCGG 1 cut(s) 1138
Hin1II CATG 5 cut(s) 73, 203, 670, 765, 982
HincII GTYRAC 2 cut(s) 105, 732
HindII GTYRAC 2 cut(s) 105, 732
HindIII AAGCTT 1 cut(s) 906
HinfI GANTC 6 cut(s) 267, 443, 595, 787, 1038, 1057
HpaII CCGG 1 cut(s) 1138
HphI GGTGA 2 cut(s) 438, 452
Hpy166II GTNNAC 6 cut(s) 105, 196, 246, 584, 732, 1147
Hpy188I TCNGA 5 cut(s) 274, 777, 792, 917, 1047
Hpy188III TCNNGA 9 cut(s) 142, 323, 548, 740, 814, 986, 1061, 1127, 1288
Hpy8I GTNNAC 6 cut(s) 105, 196, 246, 584, 732, 1147
Hpy99I CGWCG 1 cut(s) 1167
HpyAV CCTTC 3 cut(s) 289, 622, 737
HpyCH4III ACNGT 4 cut(s) 26, 102, 390, 1276
HpyCH4IV ACGT 3 cut(s) 490, 696, 1162
HpyF10VI GCNNNNNNNGC 1 cut(s) 1177
HpyF3I CTNAG 4 cut(s) 271, 301, 671, 960
HpySE526I ACGT 3 cut(s) 490, 696, 1162
Hsp92II CATG 5 cut(s) 73, 203, 670, 765, 982
Kzo9I GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 2 cut(s) 576, 1253
LweI GCATC 2 cut(s) 126, 1057
MaeI CTAG 3 cut(s) 437, 521, 986
MaeII ACGT 3 cut(s) 490, 696, 1162
MaeIII GTNAC 2 cut(s) 601, 941
MalI GATC 6 cut(s) 552, 645, 684, 965, 990, 1079
MboI GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
MboII GAAGA 5 cut(s) 57, 904, 983, 1072, 1076
MflI RGATCY 3 cut(s) 643, 988, 1077
MhlI GDGCHC 2 cut(s) 198, 586
MluCI AATT 8 cut(s) 91, 134, 231, 362, 771, 809, 851, 1048
MlyI GAGTC 3 cut(s) 276, 589, 796
MnlI CCTC 9 cut(s) 8, 214, 266, 319, 451, 487, 629, 740, 793
MseI TTAA 4 cut(s) 234, 710, 807, 1294
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 1 cut(s) 1138
MspR9I CCNGG 1 cut(s) 1139
Mva1269I GAATGC 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 1177
NciI CCSGG 1 cut(s) 1139
NcoI CCATGG 1 cut(s) 199
NdeII GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
NlaIII CATG 5 cut(s) 73, 203, 670, 765, 982
NlaIV GGNNCC 1 cut(s) 953
NmeAIII GCCGAG 1 cut(s) 1197
OliI CACNNNNGTG 1 cut(s) 290
PaeR7I CTCGAG 2 cut(s) 264, 784
PceI AGGCCT 1 cut(s) 481
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 3 cut(s) 443, 1038, 1057
PflFI GACNNNGTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 2 cut(s) 566, 1243
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 3 cut(s) 275, 589, 795
PpsI GAGTC 3 cut(s) 275, 589, 795
Ppu21I YACGTR 2 cut(s) 491, 697
PspN4I GGNNCC 1 cut(s) 953
PspPI GGNCC 1 cut(s) 188
PspXI VCTCGAGB 2 cut(s) 264, 784
PsuI RGATCY 3 cut(s) 643, 988, 1077
PsyI GACNNNGTC 1 cut(s) 302
PvuI CGATCG 1 cut(s) 553
RsaI GTAC 4 cut(s) 577, 699, 826, 867
RsaNI GTAC 4 cut(s) 576, 698, 825, 866
RseI CAYNNNNRTG 1 cut(s) 290
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 4 cut(s) 234, 710, 807, 1294
SatI GCNGC 2 cut(s) 565, 1242
Sau3AI GATC 6 cut(s) 550, 643, 682, 963, 988, 1077
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 3 cut(s) 276, 589, 796
ScrFI CCNGG 1 cut(s) 1139
SduI GDGCHC 2 cut(s) 198, 586
SfaNI GCATC 2 cut(s) 126, 1057
SfcI CTRYAG 1 cut(s) 45
Sfr274I CTCGAG 2 cut(s) 264, 784
SlaI CTCGAG 2 cut(s) 264, 784
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 6 cut(s) 264, 323, 597, 701, 784, 1230
SmoI CTYRAG 6 cut(s) 264, 323, 597, 701, 784, 1230
Sse9I AATT 8 cut(s) 91, 134, 231, 362, 771, 809, 851, 1048
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 1 cut(s) 337
SspMI CTAG 3 cut(s) 437, 521, 986
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 1137
StyI CCWWGG 2 cut(s) 199, 536
TaaI ACNGT 4 cut(s) 26, 102, 390, 1276
TaiI ACGT 3 cut(s) 493, 699, 1165
TaqI TCGA 8 cut(s) 104, 265, 417, 549, 553, 785, 830, 894
TasI AATT 8 cut(s) 91, 134, 231, 362, 771, 809, 851, 1048
TatI WGTACW 1 cut(s) 824
TfiI GAWTC 3 cut(s) 443, 1038, 1057
Tru1I TTAA 4 cut(s) 234, 710, 807, 1294
Tru9I TTAA 4 cut(s) 234, 710, 807, 1294
TscAI CASTG 5 cut(s) 198, 363, 511, 944, 1281
TseI GCWGC 2 cut(s) 564, 1241
TspDTI ATGAA 3 cut(s) 86, 168, 655
TspGWI ACGGA 5 cut(s) 416, 668, 809, 1050, 1203
TspRI CASTG 5 cut(s) 198, 363, 511, 944, 1281
Tth111I GACNNNGTC 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 473
VneI GTGCAC 2 cut(s) 194, 582
XapI RAATTY 4 cut(s) 91, 362, 809, 851
XbaI TCTAGA 1 cut(s) 985
XhoI CTCGAG 2 cut(s) 264, 784
XmiI GTMKAC 2 cut(s) 104, 245
XspI CTAG 3 cut(s) 437, 521, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.