RLG00000008853

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011564
Physical Location & Seq
Reverse (-)
41184826 .. 41186340
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008853

Sequence Viewer

Length: 1515 bp
ATGGAAGGTCTTGGTAGAGATGATAGCGCTAATGATTTTTGTCCAATTGAAATGAGCAGTTTCGGATCTTCCACAGGATATAATGTTGATACTGCAGCTGCACCACATGAGAGTAGTTTCCGATCGTCCATGGGAAATAATATTGATGTTGCAGCTGCATCATACGAAAGTAGTTTTGGATCTTCTGTGGGAAACATTGATATTGGATTTGTGGAAAGGGATTTGAGATCTTCACTGGATAGTATTGTTTTTGAAAACTCCCTCTTGTCGACCAAATGTTCCATCTTCGAAACCCCCAAAATACTCTTTAGAACTAAAGAGAAAACTTATGTCCCCACGGCATTCTCGATCGGGCCTTTACACCATGGCAAACCAGGCTTGACAGCCACAGAACAAATTAAACTGAAATATACGCAAGACCTCATCAGTCGAGTCTCACAAACAAAGGGCACGGTTTTGAAAGACATGGTAAAATGGATTGCATGTACTGAGGAAGAAGCCCGCCAATGTTATGCTGTACCAGTTGAATTCACTAGAAATGAGTTTATCAAAATCTTGTTAGTTGATGGTTGCTTTCTCATCGAGCTATTCCGTAAATGTGGAGGTCTTGATTCATCGACTTCAGCGAATGACCCCGTGATGAACAGGGCTTGTATGCTTGAGGTTGTAGATCGTGACTTGTTCTTGTTAGAAAACCAAATACCTTGGATGGTGCTTCATCGTCTGTTTAGCTTGATTTCTTCTGATGACGACATTCTTTTCTTTCGTTCTCCACAGCCAATGTCGTCTGGTAATGTCCTAACTAGACTAGCTTTGAGGTTCTTTCGAATTTTTGGTACAAGGGGGTTAGATTTATCAATGGAAACTAGCTTAATGCGAAGCAGTCATTATAACGAAACTAAACACGTACATGTACTTGATCTGCTGAGATCTTCGATAATAGGGTTAACGTCAAGATTAGAAAGCGGAAATAAGGAAAGATGGGAACTAATTCCAAGTGCCTCAAGCCTGATAGAAGCTGGAGTGAAATTCAAGAAGGGTAGTACGGATTGTGGCATATTGGACATCAAATTCAGTAATGGAGTACTCGAAATACCACCTTTCATTATTCACGAGTCAACTGAAGCTTTCTTCCGTAACATGATCAGCTTCGAGCAGTGTTACCATCTGTGCGATCCTAGGATCACAAATTATGCCATGCTACTAGATATACTCATCAACACTCCAAGAGATATAGATATACTCTGCGAATATGGAATCATTAAGAATTGGTTGAATCCAGAAGAGGCATCCCATATATTTGACAACCTTGACAGTGATACATATGTCACCGAGTTCTATTACCTAGAGCTTTCCCAGGAAGTTAACAAATATTGCAAGCGTCGTTGGCCAAGATGGCGAGCAATGTATGTTCGAAATCATTTCAGCAGCCCATGGGCAATCGCCTCGCAAATGTTGGCAGCTATCATATTTATTCTTGCATTATTGCAAGCAGTGTTCACCATCAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

505

Amino Acids

57.14

Weight (kDa)

5.58

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 95 - 493 2.2e-104 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 891
AccI GTMKAC 1 cut(s) 269
AciI CCGC 2 cut(s) 502, 966
AclWI GGATC 4 cut(s) 73, 187, 1169, 1190
AcoI YGGCCR 1 cut(s) 1388
AcsI RAATTY 4 cut(s) 527, 828, 1028, 1070
AcuI CTGAAG 2 cut(s) 606, 1143
AfaI GTAC 7 cut(s) 487, 519, 838, 909, 915, 1045, 1086
AfeI AGCGCT 1 cut(s) 28
AflIII ACRYGT 2 cut(s) 904, 910
AgsI TTSAA 6 cut(s) 50, 254, 460, 527, 1033, 1276
AjnI CCWGG 2 cut(s) 373, 1356
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
Alw26I GTCTC 1 cut(s) 439
AlwI GGATC 4 cut(s) 73, 187, 1169, 1190
Aor51HI AGCGCT 1 cut(s) 28
AoxI GGCC 2 cut(s) 353, 1388
ApeKI GCWGC 6 cut(s) 95, 98, 152, 155, 1428, 1460
ApoI RAATTY 4 cut(s) 527, 828, 1028, 1070
Asp700I GAANNNNTTC 1 cut(s) 990
AspA2I CCTAGG 1 cut(s) 1178
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 1321, 1492
AsuII TTCGAA 3 cut(s) 288, 826, 1414
AvrII CCTAGG 1 cut(s) 1178
BaeGI GKGCMC 1 cut(s) 452
BaeI ACNNNNGTAYC 2 cut(s) 1311, 1344
BalI TGGCCA 1 cut(s) 1390
BauI CACGAG 1 cut(s) 1112
BbvI GCAGC 6 cut(s) 85, 107, 142, 164, 1440, 1472
BccI CCATC 7 cut(s) 290, 560, 703, 975, 1173, 1389, 1511
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 2 cut(s) 1428, 1462
BciT130I CCWGG 2 cut(s) 375, 1358
BclI TGATCA 1 cut(s) 1143
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 7 cut(s) 534, 804, 809, 867, 1179, 1205, 1346
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 30
BglI GCCNNNNNGGC 1 cut(s) 1396
BglII AGATCT 2 cut(s) 227, 929
BisI GCNGC 6 cut(s) 96, 99, 153, 156, 1429, 1461
BlnI CCTAGG 1 cut(s) 1178
BlsI GCNGC 6 cut(s) 97, 100, 154, 157, 1430, 1462
BmcAI AGTACT 1 cut(s) 1086
Bme1390I CCNGG 2 cut(s) 375, 1358
BmgT120I GGNCC 1 cut(s) 353
BmrFI CCNGG 2 cut(s) 375, 1358
BmsI GCATC 2 cut(s) 167, 1298
BpmI CTGGAG 1 cut(s) 1041
Bpu14I TTCGAA 3 cut(s) 288, 826, 1414
BpuEI CTTGAG 2 cut(s) 680, 988
BsaAI YACGTR 1 cut(s) 907
BsaJI CCNNGG 7 cut(s) 129, 336, 364, 704, 1178, 1356, 1433
Bse1I ACTGG 2 cut(s) 240, 521
Bse3DI GCAATG 1 cut(s) 1410
BseBI CCWGG 2 cut(s) 375, 1358
BseDI CCNNGG 7 cut(s) 129, 336, 364, 704, 1178, 1356, 1433
BseGI GGATG 2 cut(s) 714, 1289
BseMI GCAATG 1 cut(s) 1410
BseMII CTCAG 2 cut(s) 480, 917
BseNI ACTGG 2 cut(s) 240, 521
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 6 cut(s) 85, 107, 142, 164, 1440, 1472
BsgI GTGCAG 1 cut(s) 84
Bsh1285I CGRYCG 2 cut(s) 125, 351
BshFI GGCC 2 cut(s) 355, 1390
BsiEI CGRYCG 2 cut(s) 125, 351
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 317
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 2 cut(s) 355, 1390
Bsp119I TTCGAA 3 cut(s) 288, 826, 1414
Bsp1286I GDGCHC 1 cut(s) 452
Bsp19I CCATGG 3 cut(s) 129, 364, 1433
BspACI CCGC 2 cut(s) 502, 966
BspANI GGCC 2 cut(s) 355, 1390
BspCNI CTCAG 2 cut(s) 481, 918
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 4 cut(s) 73, 187, 1169, 1190
BspT104I TTCGAA 3 cut(s) 288, 826, 1414
BsrDI GCAATG 1 cut(s) 1410
BsrI ACTGG 2 cut(s) 240, 521
BssECI CCNNGG 7 cut(s) 129, 336, 364, 704, 1178, 1356, 1433
BssSI CACGAG 1 cut(s) 1112
BssT1I CCWWGG 5 cut(s) 129, 364, 704, 1178, 1433
Bst2BI CACGAG 1 cut(s) 1112
Bst2UI CCWGG 2 cut(s) 375, 1358
Bst4CI ACNGT 2 cut(s) 454, 1316
Bst6I CTCTTC 1 cut(s) 1278
BstBAI YACGTR 1 cut(s) 907
BstBI TTCGAA 3 cut(s) 288, 826, 1414
BstC8I GCNNGC 4 cut(s) 502, 1379, 1401, 1491
BstDEI CTNAG 2 cut(s) 489, 926
BstDSI CCRYGG 4 cut(s) 129, 336, 364, 1433
BstF5I GGATG 2 cut(s) 714, 1289
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstMAI GTCTC 1 cut(s) 439
BstMCI CGRYCG 2 cut(s) 125, 351
BstMWI GCNNNNNNNGC 3 cut(s) 375, 1387, 1396
BstNI CCWGG 2 cut(s) 375, 1358
BstNSI RCATGY 2 cut(s) 486, 914
BstSCI CCNGG 2 cut(s) 373, 1356
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 6 cut(s) 85, 107, 142, 164, 1440, 1472
BstX2I RGATCY 4 cut(s) 65, 179, 227, 929
BstYI RGATCY 4 cut(s) 65, 179, 227, 929
BsuRI GGCC 2 cut(s) 355, 1390
BtgI CCRYGG 4 cut(s) 129, 336, 364, 1433
BtsCI GGATG 2 cut(s) 714, 1289
BtsI GCAGTG 2 cut(s) 1163, 1500
BtsIMutI CAGTG 4 cut(s) 233, 1163, 1321, 1500
Cac8I GCNNGC 4 cut(s) 502, 1379, 1401, 1491
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 1370
Csp6I GTAC 7 cut(s) 486, 518, 837, 908, 914, 1044, 1085
CviAII CATG 9 cut(s) 107, 130, 365, 466, 483, 911, 1141, 1198, 1434
CviQI GTAC 7 cut(s) 486, 518, 837, 908, 914, 1044, 1085
DdeI CTNAG 2 cut(s) 489, 926
EaeI YGGCCR 1 cut(s) 1388
Eam1104I CTCTTC 1 cut(s) 1278
EarI CTCTTC 1 cut(s) 1278
Eco130I CCWWGG 5 cut(s) 129, 364, 704, 1178, 1433
Eco47III AGCGCT 1 cut(s) 28
Eco57I CTGAAG 2 cut(s) 606, 1143
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 373, 1356
EcoT14I CCWWGG 5 cut(s) 129, 364, 704, 1178, 1433
ErhI CCWWGG 5 cut(s) 129, 364, 704, 1178, 1433
FaeI CATG 9 cut(s) 110, 133, 368, 469, 486, 914, 1144, 1201, 1437
FaqI GGGAC 1 cut(s) 317
FatI CATG 9 cut(s) 106, 129, 364, 465, 482, 910, 1140, 1197, 1433
FauI CCCGC 1 cut(s) 509
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 1 cut(s) 1143
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 6 cut(s) 96, 99, 153, 156, 1429, 1461
FokI GGATG 2 cut(s) 721, 1276
Fsp4HI GCNGC 6 cut(s) 96, 99, 153, 156, 1429, 1461
FspBI CTAG 7 cut(s) 534, 804, 809, 867, 1179, 1205, 1346
GlaI GCGC 1 cut(s) 28
GluI GCNGC 6 cut(s) 96, 99, 153, 156, 1429, 1461
GsuI CTGGAG 1 cut(s) 1041
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 355, 1390
HgaI GACGC 1 cut(s) 1370
HhaI GCGC 1 cut(s) 29
Hin1II CATG 9 cut(s) 110, 133, 368, 469, 486, 914, 1144, 1201, 1437
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HincII GTYRAC 4 cut(s) 270, 948, 1119, 1366
HindII GTYRAC 4 cut(s) 270, 948, 1119, 1366
HindIII AAGCTT 1 cut(s) 1125
HinfI GANTC 5 cut(s) 432, 611, 1115, 1257, 1276
HpaI GTTAAC 2 cut(s) 948, 1366
HphI GGTGA 2 cut(s) 1321, 1492
Hpy166II GTNNAC 5 cut(s) 270, 948, 1119, 1366, 1500
Hpy188I TCNGA 3 cut(s) 65, 122, 745
Hpy188III TCNNGA 8 cut(s) 346, 608, 674, 954, 1033, 1112, 1280, 1507
Hpy8I GTNNAC 5 cut(s) 270, 948, 1119, 1366, 1500
Hpy99I CGWCG 1 cut(s) 1386
HpyAV CCTTC 1 cut(s) 1030
HpyCH4III ACNGT 2 cut(s) 454, 1316
HpyCH4IV ACGT 2 cut(s) 906, 950
HpyCH4V TGCA 8 cut(s) 95, 101, 152, 158, 482, 1377, 1481, 1489
HpyF10VI GCNNNNNNNGC 3 cut(s) 375, 1387, 1396
HpyF3I CTNAG 2 cut(s) 489, 926
HpySE526I ACGT 2 cut(s) 906, 950
Hsp92II CATG 9 cut(s) 110, 133, 368, 469, 486, 914, 1144, 1201, 1437
HspAI GCGC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 1143
KspAI GTTAAC 2 cut(s) 948, 1366
Lsp1109I GCAGC 6 cut(s) 85, 107, 142, 164, 1440, 1472
LweI GCATC 2 cut(s) 167, 1298
MaeI CTAG 7 cut(s) 534, 804, 809, 867, 1179, 1205, 1346
MaeII ACGT 2 cut(s) 906, 950
MaeIII GTNAC 4 cut(s) 674, 1136, 1160, 1327
MboII GAAGA 9 cut(s) 60, 174, 222, 277, 506, 732, 924, 1123, 1295
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 4 cut(s) 65, 179, 227, 929
MhlI GDGCHC 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 1390
MluNI TGGCCA 1 cut(s) 1390
MlyI GAGTC 2 cut(s) 441, 1124
MnlI CCTC 9 cut(s) 272, 431, 484, 596, 655, 810, 1012, 1279, 1456
Mox20I TGGCCA 1 cut(s) 1390
MroXI GAANNNNTTC 1 cut(s) 990
MscI TGGCCA 1 cut(s) 1390
MseI TTAA 6 cut(s) 399, 872, 947, 1263, 1365, 1513
MslI CAYNNNNRTG 1 cut(s) 909
Msp20I TGGCCA 1 cut(s) 1390
MspA1I CMGCKG 2 cut(s) 98, 155
MspR9I CCNGG 2 cut(s) 375, 1358
MunI CAATTG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 2 cut(s) 375, 1358
MwoI GCNNNNNNNGC 3 cut(s) 375, 1387, 1396
NcoI CCATGG 3 cut(s) 129, 364, 1433
NdeI CATATG 1 cut(s) 1324
NlaIII CATG 9 cut(s) 110, 133, 368, 469, 486, 914, 1144, 1201, 1437
NmuCI GTSAC 2 cut(s) 674, 1327
NspI RCATGY 2 cut(s) 486, 914
NspV TTCGAA 3 cut(s) 288, 826, 1414
PciI ACATGT 1 cut(s) 910
PcsI WCGNNNNNNNCGW 2 cut(s) 344, 623
PctI GAATGC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 611, 1257, 1276
PkrI GCNGC 6 cut(s) 97, 100, 154, 157, 1430, 1462
Ple19I CGATCG 2 cut(s) 125, 351
PleI GAGTC 2 cut(s) 440, 1123
PpsI GAGTC 2 cut(s) 440, 1123
Ppu21I YACGTR 1 cut(s) 907
PscI ACATGT 1 cut(s) 910
PsiI TTATAA 1 cut(s) 891
Psp6I CCWGG 2 cut(s) 373, 1356
PspGI CCWGG 2 cut(s) 373, 1356
PspPI GGNCC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 4 cut(s) 65, 179, 227, 929
PvuI CGATCG 2 cut(s) 125, 351
PvuII CAGCTG 2 cut(s) 98, 155
RsaI GTAC 7 cut(s) 487, 519, 838, 909, 915, 1045, 1086
RsaNI GTAC 7 cut(s) 486, 518, 837, 908, 914, 1044, 1085
RseI CAYNNNNRTG 1 cut(s) 909
SalI GTCGAC 1 cut(s) 268
SaqAI TTAA 6 cut(s) 399, 872, 947, 1263, 1365, 1513
SatI GCNGC 6 cut(s) 96, 99, 153, 156, 1429, 1461
Sau96I GGNCC 1 cut(s) 353
ScaI AGTACT 1 cut(s) 1086
SchI GAGTC 2 cut(s) 441, 1124
ScrFI CCNGG 2 cut(s) 375, 1358
SduI GDGCHC 1 cut(s) 452
SfaNI GCATC 2 cut(s) 167, 1298
SfcI CTRYAG 1 cut(s) 93
SfuI TTCGAA 3 cut(s) 288, 826, 1414
SmiMI CAYNNNNRTG 1 cut(s) 909
SmlI CTYRAG 2 cut(s) 659, 1003
SmoI CTYRAG 2 cut(s) 659, 1003
SsiI CCGC 2 cut(s) 502, 966
SspI AATATT 2 cut(s) 142, 1373
SspMI CTAG 7 cut(s) 534, 804, 809, 867, 1179, 1205, 1346
StyD4I CCNGG 2 cut(s) 373, 1356
StyI CCWWGG 5 cut(s) 129, 364, 704, 1178, 1433
TaaI ACNGT 2 cut(s) 454, 1316
TaiI ACGT 2 cut(s) 909, 953
TatI WGTACW 3 cut(s) 485, 913, 1084
TfiI GAWTC 3 cut(s) 611, 1257, 1276
Tru1I TTAA 6 cut(s) 399, 872, 947, 1263, 1365, 1513
Tru9I TTAA 6 cut(s) 399, 872, 947, 1263, 1365, 1513
TscAI CASTG 4 cut(s) 240, 1163, 1321, 1500
TseFI GTSAC 2 cut(s) 674, 1327
TseI GCWGC 6 cut(s) 95, 98, 152, 155, 1428, 1460
Tsp45I GTSAC 2 cut(s) 674, 1327
TspDTI ATGAA 4 cut(s) 603, 656, 707, 1093
TspGWI ACGGA 3 cut(s) 581, 1061, 1124
TspRI CASTG 4 cut(s) 240, 1163, 1321, 1500
XapI RAATTY 4 cut(s) 527, 828, 1028, 1070
XceI RCATGY 2 cut(s) 486, 914
XmaJI CCTAGG 1 cut(s) 1178
XmiI GTMKAC 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 7 cut(s) 534, 804, 809, 867, 1179, 1205, 1346
ZrmI AGTACT 1 cut(s) 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.