RLG00000002978

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011564
Physical Location & Seq
Reverse (-)
42586158 .. 42587453
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002978

Sequence Viewer

Length: 1296 bp
ATGGAGAGTTTTGGAGGAGATGATGGTGCAGTTGACATTGCAACTATAGGAAGTGATTTGATATCTTCCATGAACAACATTGTGTCTGAAAATTCTTTACTGTCGACCAAATGTTGCATCTTCGCAACCCCAAAATTACTCTTGAAAGATAATGAAAAATCTTTTTTACCCAAGGCATTCGCCATTGGGCCAGTGCACCATGGCACACCAGACTTGCAGGAGGCAGAGCAAATTAAACTGAAGTATACACGAGACCTCATTGATCGAGTCTCAGAATGGAATGACACGATTGTGAAGGACTTGGTCGAAGAGATTGCAAGTATTGAGGAACAAGCCCGCCAATACTACGCTGTGCCAGTGAAATTCAATAAAGCAAAGTTTGTAAAAATGTTGTTGGTTGATGGTTGCTTTATCATCGAGCTGTTCCGTAAAGAGCTAGGTGATTCACCTGTTTATGAGGATGACCCAGTAATTGGTAGGCCTTTCTTACGCGAGGTATTAGACACTGACTTGTTATTACTAGAAAACCAAATACCTTGGATGGTGCTCGATCGATTGTTTAGCAGGACAACTCGTACTGGTGCAAGTCCCCTGACTCGACTAACCTTATTCTTCTTTAATTTCTTGGGAACAGGAGGATTAGATCTAGAAACAGAAGATAGTTTCATGCCCAGTACTTTAGATCAAAGCAAGCACATACTAGAGCTTTTAAGGAACTCATTAGTTTGGTCAACTATGCCAGAAGGAGGCGCGCTTGATACATGGGAGCTAATTCCGAGTGCCTCCAGTCTGATGGAAGCTGGTGTTACATTCAAGAAGGGTAGTACATCGAATGGCTTATTGGATATCAAATTCAGTAATGGGGTACTCGAAATACCACCTTTGGTTATTGTCGACTCGACAAAAGCTTTCTTCCGAAACTTGATTTGCTATGAGCAGTGTTACCATTGGTGCCATCCTAGGATCACTTCTTATGCCATGCTTCTAGATCTTCTCATCAACACTCCAAAAGATATGGATATACTCTGCAAAAACGGAATCATTCAAAATTGGTTGAATCCAGAAGATGCGACCCAGATCTTCAACAACCTTTACAATGATACAAATGTCAACCACTTCTATTATCTTGAACTTTGCCGGGAAGTGAACAAGTATTGCAAACGTCGTTGGCCGAGATGGCGAGCAATGTATGTGCGAAATCATTTCAGCAGTCCATGGGCAATTGCTTCCCAAGTTTTGGCAGCTATCATATTTATTCTCACATTGTTGCAAACAGTGTTTAGCATCAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

432

Amino Acids

49.11

Weight (kDa)

5.18

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 40 - 420 4.5e-103 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 951
AccB7I CCANNNNNTGG 2 cut(s) 473, 1237
AccI GTMKAC 3 cut(s) 104, 245, 894
AccII CGCG 2 cut(s) 492, 752
AciI CCGC 1 cut(s) 337
AclWI GGATC 1 cut(s) 971
AcoI YGGCCR 1 cut(s) 1169
AcsI RAATTY 3 cut(s) 91, 362, 851
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 4 cut(s) 577, 676, 826, 867
AfiI CCNNNNNNNGG 3 cut(s) 473, 746, 1237
AgsI TTSAA 7 cut(s) 145, 367, 814, 1046, 1057, 1084, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 7 cut(s) 421, 436, 706, 769, 800, 908, 1244
AluI AGCT 7 cut(s) 421, 436, 706, 769, 800, 908, 1244
Alw21I GWGCWC 2 cut(s) 198, 549
Alw26I GTCTC 2 cut(s) 246, 274
Alw44I GTGCAC 1 cut(s) 194
AlwI GGATC 1 cut(s) 971
AoxI GGCC 3 cut(s) 188, 479, 1169
ApaLI GTGCAC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 3 cut(s) 91, 362, 851
AspA2I CCTAGG 1 cut(s) 959
AspLEI GCGC 2 cut(s) 752, 754
AspS9I GGNCC 1 cut(s) 188
AsuC2I CCSGG 1 cut(s) 1139
AsuHPI GGTGA 2 cut(s) 438, 452
AvrII CCTAGG 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BanI GGYRCC 1 cut(s) 951
BauI CACGAG 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 198, 549
BbvI GCAGC 1 cut(s) 1253
BccI CCATC 6 cut(s) 17, 395, 535, 787, 963, 1170
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BcnI CCSGG 1 cut(s) 1139
BcoDI GTCTC 2 cut(s) 246, 274
BfaI CTAG 6 cut(s) 437, 521, 647, 701, 960, 986
BfmI CTRYAG 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 1177
BglII AGATCT 3 cut(s) 643, 988, 1077
BisI GCNGC 1 cut(s) 1242
BlnI CCTAGG 1 cut(s) 959
BlsI GCNGC 1 cut(s) 1243
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 1 cut(s) 1139
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 953
BmrFI CCNGG 1 cut(s) 1139
BmrI ACTGGG 2 cut(s) 461, 666
BmsI GCATC 2 cut(s) 126, 1057
BmuI ACTGGG 2 cut(s) 461, 666
BpmI CTGGAG 1 cut(s) 769
BpuMI CCSGG 1 cut(s) 1139
Bsa29I ATCGAT 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 5 cut(s) 171, 199, 536, 959, 1214
Bsc4I CCNNNNNNNGG 3 cut(s) 473, 746, 1237
Bse1I ACTGG 6 cut(s) 191, 356, 467, 583, 672, 786
Bse3DI GCAATG 2 cut(s) 36, 1191
BseCI ATCGAT 1 cut(s) 553
BseDI CCNNGG 5 cut(s) 171, 199, 536, 959, 1214
BseGI GGATG 3 cut(s) 466, 546, 955
BseLI CCNNNNNNNGG 3 cut(s) 473, 746, 1237
BseMI GCAATG 2 cut(s) 36, 1191
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 6 cut(s) 191, 356, 467, 583, 672, 786
BsePI GCGCGC 1 cut(s) 750
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 2 cut(s) 492, 752
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 3 cut(s) 190, 481, 1171
BshNI GGYRCC 1 cut(s) 951
BshVI ATCGAT 1 cut(s) 553
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 198, 549
BsiSI CCGG 1 cut(s) 1138
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 3 cut(s) 473, 746, 1237
BsmAI GTCTC 2 cut(s) 246, 274
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 3 cut(s) 190, 481, 1171
Bso31I GGTCTC 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 198, 549
Bsp143I GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
Bsp19I CCATGG 2 cut(s) 199, 1214
BspACI CCGC 1 cut(s) 337
BspANI GGCC 3 cut(s) 190, 481, 1171
BspCNI CTCAG 1 cut(s) 284
BspDI ATCGAT 1 cut(s) 553
BspFNI CGCG 2 cut(s) 492, 752
BspLI GGNNCC 1 cut(s) 953
BspPI GGATC 1 cut(s) 971
BspT107I GGYRCC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 36, 1191
BsrI ACTGG 6 cut(s) 191, 356, 467, 583, 672, 786
BssECI CCNNGG 5 cut(s) 171, 199, 536, 959, 1214
BssHII GCGCGC 1 cut(s) 750
BssMI GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
BssNAI GTATAC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 5 cut(s) 171, 199, 536, 959, 1214
Bst1107I GTATAC 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 102, 1276
Bst6I CTCTTC 1 cut(s) 303
BstC8I GCNNGC 4 cut(s) 337, 692, 752, 1182
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 2 cut(s) 199, 1214
BstF5I GGATG 3 cut(s) 466, 546, 955
BstFNI CGCG 2 cut(s) 492, 752
BstHHI GCGC 2 cut(s) 752, 754
BstKTI GATC 7 cut(s) 265, 553, 646, 685, 966, 991, 1080
BstMAI GTCTC 2 cut(s) 246, 274
BstMBI GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 1 cut(s) 1177
BstSCI CCNGG 1 cut(s) 1137
BstSFI CTRYAG 1 cut(s) 45
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 2 cut(s) 492, 752
BstV1I GCAGC 1 cut(s) 1253
BstX2I RGATCY 3 cut(s) 643, 988, 1077
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 3 cut(s) 643, 988, 1077
BstZ17I GTATAC 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 553
BsuRI GGCC 3 cut(s) 190, 481, 1171
BsuTUI ATCGAT 1 cut(s) 553
BtgI CCRYGG 2 cut(s) 199, 1214
BtsCI GGATG 3 cut(s) 466, 546, 955
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 5 cut(s) 198, 363, 504, 944, 1281
Cac8I GCNNGC 4 cut(s) 337, 692, 752, 1182
CfoI GCGC 2 cut(s) 752, 754
Cfr13I GGNCC 1 cut(s) 188
ClaI ATCGAT 1 cut(s) 553
Csp6I GTAC 4 cut(s) 576, 675, 825, 866
CviAII CATG 6 cut(s) 70, 200, 667, 762, 979, 1215
CviQI GTAC 4 cut(s) 576, 675, 825, 866
DdeI CTNAG 1 cut(s) 271
DpnI GATC 7 cut(s) 264, 552, 645, 684, 965, 990, 1079
DpnII GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
EaeI YGGCCR 1 cut(s) 1169
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 5 cut(s) 171, 199, 536, 959, 1214
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 246
Eco32I GATATC 2 cut(s) 63, 847
Eco57I CTGAAG 1 cut(s) 260
EcoRV GATATC 2 cut(s) 63, 847
EcoT14I CCWWGG 5 cut(s) 171, 199, 536, 959, 1214
ErhI CCWWGG 5 cut(s) 171, 199, 536, 959, 1214
FaeI CATG 6 cut(s) 73, 203, 670, 765, 982, 1218
FaqI GGGAC 1 cut(s) 573
FatI CATG 6 cut(s) 69, 199, 666, 761, 978, 1214
FauI CCCGC 1 cut(s) 344
FblI GTMKAC 3 cut(s) 104, 245, 894
Fnu4HI GCNGC 1 cut(s) 1242
FokI GGATG 3 cut(s) 473, 553, 942
Fsp4HI GCNGC 1 cut(s) 1242
FspBI CTAG 6 cut(s) 437, 521, 647, 701, 960, 986
GlaI GCGC 2 cut(s) 751, 753
GluI GCNGC 1 cut(s) 1242
GsuI CTGGAG 1 cut(s) 769
HaeIII GGCC 3 cut(s) 190, 481, 1171
HapII CCGG 1 cut(s) 1138
HhaI GCGC 2 cut(s) 752, 754
Hin1II CATG 6 cut(s) 73, 203, 670, 765, 982, 1218
Hin6I GCGC 2 cut(s) 750, 752
HinP1I GCGC 2 cut(s) 750, 752
HincII GTYRAC 5 cut(s) 34, 105, 732, 895, 1111
HindII GTYRAC 5 cut(s) 34, 105, 732, 895, 1111
HindIII AAGCTT 1 cut(s) 906
HinfI GANTC 6 cut(s) 267, 443, 595, 896, 1038, 1057
HpaII CCGG 1 cut(s) 1138
HphI GGTGA 2 cut(s) 438, 452
Hpy166II GTNNAC 8 cut(s) 34, 105, 196, 246, 732, 895, 1111, 1147
Hpy188I TCNGA 5 cut(s) 88, 274, 777, 792, 917
Hpy188III TCNNGA 7 cut(s) 142, 647, 814, 986, 1061, 1127, 1288
Hpy8I GTNNAC 8 cut(s) 34, 105, 196, 246, 732, 895, 1111, 1147
Hpy99I CGWCG 1 cut(s) 1167
HpyAV CCTTC 3 cut(s) 289, 737, 811
HpyCH4III ACNGT 2 cut(s) 102, 1276
HpyCH4IV ACGT 1 cut(s) 1162
HpyF10VI GCNNNNNNNGC 1 cut(s) 1177
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 1162
Hsp92II CATG 6 cut(s) 73, 203, 670, 765, 982, 1218
HspAI GCGC 2 cut(s) 750, 752
Kzo9I GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 1 cut(s) 1253
LweI GCATC 2 cut(s) 126, 1057
MaeI CTAG 6 cut(s) 437, 521, 647, 701, 960, 986
MaeII ACGT 1 cut(s) 1162
MaeIII GTNAC 2 cut(s) 805, 941
MalI GATC 7 cut(s) 264, 552, 645, 684, 965, 990, 1079
MboI GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
MboII GAAGA 9 cut(s) 57, 112, 320, 604, 668, 904, 983, 1072, 1076
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 3 cut(s) 643, 988, 1077
MhlI GDGCHC 2 cut(s) 198, 549
MlyI GAGTC 3 cut(s) 276, 589, 890
MnlI CCTC 9 cut(s) 8, 214, 266, 319, 451, 487, 629, 740, 793
MseI TTAA 4 cut(s) 234, 618, 710, 1294
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 1 cut(s) 1138
MspR9I CCNGG 1 cut(s) 1139
MunI CAATTG 1 cut(s) 1221
Mva1269I GAATGC 1 cut(s) 176
MvnI CGCG 2 cut(s) 492, 752
MwoI GCNNNNNNNGC 1 cut(s) 1177
NciI CCSGG 1 cut(s) 1139
NcoI CCATGG 2 cut(s) 199, 1214
NdeII GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
NlaIII CATG 6 cut(s) 73, 203, 670, 765, 982, 1218
NlaIV GGNNCC 1 cut(s) 953
NmeAIII GCCGAG 1 cut(s) 1197
OliI CACNNNNGTG 1 cut(s) 290
PauI GCGCGC 1 cut(s) 750
PceI AGGCCT 1 cut(s) 481
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 3 cut(s) 443, 1038, 1057
PflFI GACNNNGTC 1 cut(s) 302
PflMI CCANNNNNTGG 2 cut(s) 473, 1237
PkrI GCNGC 1 cut(s) 1243
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 3 cut(s) 275, 589, 890
PpsI GAGTC 3 cut(s) 275, 589, 890
PspN4I GGNNCC 1 cut(s) 953
PspPI GGNCC 1 cut(s) 188
PsuI RGATCY 3 cut(s) 643, 988, 1077
PsyI GACNNNGTC 1 cut(s) 302
PteI GCGCGC 1 cut(s) 750
PvuI CGATCG 1 cut(s) 553
RsaI GTAC 4 cut(s) 577, 676, 826, 867
RsaNI GTAC 4 cut(s) 576, 675, 825, 866
RseI CAYNNNNRTG 1 cut(s) 290
SalI GTCGAC 2 cut(s) 103, 893
SaqAI TTAA 4 cut(s) 234, 618, 710, 1294
SatI GCNGC 1 cut(s) 1242
Sau3AI GATC 7 cut(s) 262, 550, 643, 682, 963, 988, 1077
Sau96I GGNCC 1 cut(s) 188
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 3 cut(s) 276, 589, 890
ScrFI CCNGG 1 cut(s) 1139
SduI GDGCHC 2 cut(s) 198, 549
SfaNI GCATC 2 cut(s) 126, 1057
SfcI CTRYAG 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 290
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 1 cut(s) 337
SspMI CTAG 6 cut(s) 437, 521, 647, 701, 960, 986
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 1137
StyI CCWWGG 5 cut(s) 171, 199, 536, 959, 1214
TaaI ACNGT 2 cut(s) 102, 1276
TaiI ACGT 1 cut(s) 1165
TatI WGTACW 2 cut(s) 674, 824
TfiI GAWTC 3 cut(s) 443, 1038, 1057
Tru1I TTAA 4 cut(s) 234, 618, 710, 1294
Tru9I TTAA 4 cut(s) 234, 618, 710, 1294
TscAI CASTG 5 cut(s) 198, 363, 511, 944, 1281
TseI GCWGC 1 cut(s) 1241
TspDTI ATGAA 3 cut(s) 86, 168, 655
TspGWI ACGGA 2 cut(s) 416, 1050
TspRI CASTG 5 cut(s) 198, 363, 511, 944, 1281
Tth111I GACNNNGTC 1 cut(s) 302
Van91I CCANNNNNTGG 2 cut(s) 473, 1237
VneI GTGCAC 1 cut(s) 194
XapI RAATTY 3 cut(s) 91, 362, 851
XbaI TCTAGA 2 cut(s) 646, 985
XmaJI CCTAGG 1 cut(s) 959
XmiI GTMKAC 3 cut(s) 104, 245, 894
XspI CTAG 6 cut(s) 437, 521, 647, 701, 960, 986
ZrmI AGTACT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.