Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005432

Physical Location & Seq

Genomic Coordinates Reverse (-)

14896409 .. 14899950

Gene Structure 3542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g23550.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGCTCAACACCCATTTCTCTTTGTTTCCTTCTGCAATGGCTAAAACCTCAAGCAACAGCTTAAACCCTGAAAAACATAACTCTTTGGCTACCCAACAACAACAGCAACAACAACATCCATGGTGGTTTTCACCAGAAACAGGAATCTACAGCAGCAAACACCCTAATGTTAACCTCCCCAGTGACCCTTTTCTTGATGTTGTCTCATTCATTTTCTCACACAAGCACAGTGGGGTTTCAGCTTTGATTGATTCGTCATCTGGGTTTTCAATATCTTACTCAAAGCTCTATTCTTTGGTCGAGTCCATGGCCTCTGGCCTCATCAAAATGGGTGTTTCACAAGGTGATGTTGTTCTGCTTCTTTTGCCTAATTCTGTCTACTATCCCATTGTTTTCTTGGGTGTTTTGTATATTGGTGCTGTTGTTACAACCATGAACCCTCTAAGCAGTGTGGTAGAGATTAAGAAACAGATTAAGGATTGCAATGCATGTTTTGGTTTCACAGGATATGAAAATGTTGATAAGTTGCAAGCATTGAGTATTCCTGCAATTGCAGTTCCAGGAAATGTAGTATTGGATTCAAAGAAAGAAATGTTTTCAGTTTTTTATCAGCTTGTTGATAGTGAAGTTGATTTGGCTCTAAGGCCAGTGATCAAGCAGCAAGACACTGCTGCGATATTGTATTCATCCGGGACTACTGGTGTGAGTAAAGGTGTTTTGATAACACATGGGAATTTGATAGCTACAGTGGAGCTTTTTGTGCGGTTTGAGGCTTCACAATATGAGTATTCAAGCTTGAAGAATGTGTATTTATCTGTTCTGCCATTGTTTCATATATACGGGTTGGCACTTATGGTGGTTGGATTATTGTCATTGGGGTCGAGCATTGTCATAATGAAGAAATTTGATGTTAATGAGGTGGTCTCAGCAATTGATAGATATAAAGTGACACATTTCCCAGTTGTTCCACCAATATTAACAGCATTAACAAAAATAGCACAGGATGTTGGGGCACAAAGTCTGCAGAGTTTAAAGCAGGTTTCTTGTGGAGCAGCTCCAACGAGCATGAAACTCATAGAGGAGTTAGTTCAGACCCTTCCTCATGTTGATTTTATTCAGGGATATGGCATGACTGAGACGACTGCAGTAGGAACTCGTGGCTTCAATACTGAAAAGATCAGGAAATATTCTTCAATTGGACTTTTAGCTCCAAACATGCAAGCTAAAGTGGTAGATTGGAATACGGGATCCTTTTTGCCTCCAGCTAGTATCGGTGAACTATGGTTACGAGGACCTTCAATTATGAGAGGTTACTTGAATAATGCAAGGGCAACTATGTCAACAATTGATAATGATGGTTGGCTGCATACCGGGGATATCAGTTATTTTGATGAGGACGGATACTTGTATATATGTGATCGCATAAAAGAGATTATCAAATACAAGGGATTTCAGATTGCTCCTGCAGATTTAGAAGCTGTGTTGATTTCGCATCCTGAGATACTAGATGTTGGAGTTACAGGTGCTACCGATGAAGAATGTGGAGAGGTACCAGTGGCATTTGTGGTTAGGAAGCATGACAGTGAACTTTCTCAGGAAGATATCATGGATTACGTTGCCCGACAGGTTTCTCCACACAAGAAGGTCAGAAAGGTGGTGTTTACACATTCAATACCAAGATCTGCAGCAGGGAAGATCCTGCGAAGGGAGCTCAGAAATCTCTTGGCTTCTAGACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006732 GO:0006733 GO:0006743 GO:0006744 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0042180 GO:0042181 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

580

Amino Acids

63.64

Weight (kDa)

6.44

Isoelectric Point (pI)

39.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	75 - 439	8.5e-87	AMP-binding enzyme
AMP-binding_C	PF13193	491 - 565	6.2e-17	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19010
fragaria_vesca	FvH4_5g23550
malus_domestica	MD14G1050700.v1.1
prunus_persica	Prupe.7G085200_v2.0.a1 Prupe.8G160600_v2.0.a1
pyrus_communis	pycom12g04570 pycom14g04200
rosa_chinensis	RchiOBHm_Chr3g0478721 RchiOBHm_Chr7g0212861
rosa_laevigata	RLG00000002864 RLG00000023630
rosa_multiflora	Rmu_sc0000394.1_g000050 Rmu_sc0001519.1_g000013
rosa_roxburghii	Rroxscaffold_3G00246500 Rroxscaffold_6G00403160
rosa_rugosa	Rorug03G0167300 Rorug03G0167400 Rorug07G0137100
rosa_samantha	Rh3AG219500 Rh3BG251200 Rh3CG248100 Rh3CG248200 Rh3DG244500 Rh7BG264300 Rh7CG287700 Rh7DG276800
rosa_wichuraiana	Rw3G019720 Rw7G023220 Rw7G023340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1027
Acc65I	GGTACC	1 cut(s)	1549
AccB1I	GGYRCC	1 cut(s)	1549
AccI	GTMKAC	1 cut(s)	378
AciI	CCGC	1 cut(s)	763
AclWI	GGATC	3 cut(s)	1242, 1255, 1690
AcsI	RAATTY	2 cut(s)	733, 902
AdeI	CACNNNGTG	1 cut(s)	344
AfaI	GTAC	1 cut(s)	1551
AfiI	CCNNNNNNNGG	2 cut(s)	140, 1705
AgsI	TTSAA	9 cut(s)	270, 582, 792, 799, 1165, 1194, 1299, 1318, 1671
AjnI	CCWGG	1 cut(s)	559
Alw21I	GWGCWC	1 cut(s)	1713
Alw26I	GTCTC	3 cut(s)	208, 928, 1130
AlwI	GGATC	3 cut(s)	1242, 1255, 1690
AoxI	GGCC	3 cut(s)	309, 316, 644
ApeKI	GCWGC	6 cut(s)	153, 658, 671, 1052, 1363, 1685
ApoI	RAATTY	2 cut(s)	733, 902
Asp718I	GGTACC	1 cut(s)	1549
AspS9I	GGNCC	1 cut(s)	1292
AsuC2I	CCSGG	2 cut(s)	691, 1372
AsuHPI	GGTGA	3 cut(s)	123, 356, 1286
AvaII	GGWCC	1 cut(s)	1292
BaeGI	GKGCMC	1 cut(s)	1015
BamHI	GGATCC	1 cut(s)	1247
BanI	GGYRCC	1 cut(s)	1549
BanII	GRGCYC	1 cut(s)	1713
BauI	CACGAG	1 cut(s)	1155
Bbv12I	GWGCWC	1 cut(s)	1713
BbvI	GCAGC	6 cut(s)	165, 658, 670, 1064, 1350, 1697
BccI	CCATC	1 cut(s)	1349
BciT130I	CCWGG	1 cut(s)	561
BciVI	GTATCC	1 cut(s)	1394
BclI	TGATCA	1 cut(s)	651
BcnI	CCSGG	2 cut(s)	691, 1372
BcoDI	GTCTC	3 cut(s)	208, 928, 1130
BfaI	CTAG	3 cut(s)	1266, 1505, 1731
BfmI	CTRYAG	7 cut(s)	148, 744, 1022, 1143, 1464, 1683, 1736
BfuAI	ACCTGC	1 cut(s)	1027
BfuI	GTATCC	1 cut(s)	1394
BglII	AGATCT	1 cut(s)	1679
BisI	GCNGC	6 cut(s)	154, 659, 672, 1053, 1364, 1686
BlsI	GCNGC	6 cut(s)	155, 660, 673, 1054, 1365, 1687
Bme1390I	CCNGG	3 cut(s)	561, 691, 1372
Bme18I	GGWCC	1 cut(s)	1292
BmgT120I	GGNCC	1 cut(s)	1292
BmiI	GGNNCC	2 cut(s)	1249, 1551
BmrFI	CCNGG	3 cut(s)	561, 691, 1372
BmrI	ACTGGG	2 cut(s)	174, 953
BmsI	GCATC	1 cut(s)	1501
BmuI	ACTGGG	2 cut(s)	174, 953
BplI	GAGNNNNNCTC	2 cut(s)	908, 940
BpmI	CTGGAG	1 cut(s)	1245
BpuEI	CTTGAG	1 cut(s)	34
BpuMI	CCSGG	2 cut(s)	691, 1372
BsaI	GGTCTC	1 cut(s)	928
BsaJI	CCNNGG	3 cut(s)	119, 306, 1371
Bsc4I	CCNNNNNNNGG	2 cut(s)	140, 1705
Bse1I	ACTGG	5 cut(s)	180, 647, 703, 959, 1553
Bse3DI	GCAATG	2 cut(s)	42, 490
BseBI	CCWGG	1 cut(s)	561
BseDI	CCNNGG	3 cut(s)	119, 306, 1371
BseGI	GGATG	4 cut(s)	115, 686, 1009, 1492
BseLI	CCNNNNNNNGG	2 cut(s)	140, 1705
BseMI	GCAATG	2 cut(s)	42, 490
BseMII	CTCAG	5 cut(s)	939, 1125, 1488, 1607, 1726
BseNI	ACTGG	5 cut(s)	180, 647, 703, 959, 1553
BseRI	GAGGAG	1 cut(s)	1094
BseSI	GKGCMC	1 cut(s)	1015
BseXI	GCAGC	6 cut(s)	165, 658, 670, 1064, 1350, 1697
BshFI	GGCC	3 cut(s)	311, 318, 646
BshNI	GGYRCC	1 cut(s)	1549
BsiHKAI	GWGCWC	1 cut(s)	1713
BsiSI	CCGG	2 cut(s)	690, 1371
BslFI	GGGAC	1 cut(s)	706
BslI	CCNNNNNNNGG	2 cut(s)	140, 1705
BsmAI	GTCTC	3 cut(s)	208, 928, 1130
BsmBI	CGTCTC	1 cut(s)	1130
BsmFI	GGGAC	1 cut(s)	706
BsnI	GGCC	3 cut(s)	311, 318, 646
Bso31I	GGTCTC	1 cut(s)	928
Bsp1286I	GDGCHC	2 cut(s)	1015, 1713
Bsp143I	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
Bsp19I	CCATGG	2 cut(s)	119, 306
BspACI	CCGC	1 cut(s)	763
BspANI	GGCC	3 cut(s)	311, 318, 646
BspCNI	CTCAG	5 cut(s)	938, 1126, 1489, 1606, 1725
BspLI	GGNNCC	2 cut(s)	1249, 1551
BspMAI	CTGCAG	4 cut(s)	1026, 1147, 1468, 1687
BspMI	ACCTGC	1 cut(s)	1027
BspPI	GGATC	3 cut(s)	1242, 1255, 1690
BspT107I	GGYRCC	1 cut(s)	1549
BspTNI	GGTCTC	1 cut(s)	928
BsrDI	GCAATG	2 cut(s)	42, 490
BsrI	ACTGG	5 cut(s)	180, 647, 703, 959, 1553
BssECI	CCNNGG	3 cut(s)	119, 306, 1371
BssMI	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
BssSI	CACGAG	1 cut(s)	1155
BssT1I	CCWWGG	2 cut(s)	119, 306
Bst2BI	CACGAG	1 cut(s)	1155
Bst2UI	CCWGG	1 cut(s)	561
Bst4CI	ACNGT	3 cut(s)	230, 748, 1583
BstC8I	GCNNGC	2 cut(s)	531, 1221
BstDEI	CTNAG	7 cut(s)	443, 641, 925, 1134, 1497, 1593, 1712
BstDSI	CCRYGG	2 cut(s)	119, 306
BstF5I	GGATG	4 cut(s)	115, 686, 1009, 1492
BstKTI	GATC	6 cut(s)	654, 1179, 1250, 1420, 1682, 1698
BstMAI	GTCTC	3 cut(s)	208, 928, 1130
BstMBI	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
BstMWI	GCNNNNNNNGC	3 cut(s)	364, 760, 1708
BstNI	CCWGG	1 cut(s)	561
BstNSI	RCATGY	2 cut(s)	492, 1219
BstSCI	CCNGG	3 cut(s)	559, 689, 1370
BstSFI	CTRYAG	7 cut(s)	148, 744, 1022, 1143, 1464, 1683, 1736
BstSLI	GKGCMC	1 cut(s)	1015
BstV1I	GCAGC	6 cut(s)	165, 658, 670, 1064, 1350, 1697
BstX2I	RGATCY	3 cut(s)	1247, 1679, 1695
BstYI	RGATCY	3 cut(s)	1247, 1679, 1695
BsuI	GTATCC	1 cut(s)	1394
BsuRI	GGCC	3 cut(s)	311, 318, 646
BtgI	CCRYGG	2 cut(s)	119, 306
BtsCI	GGATG	4 cut(s)	115, 686, 1009, 1492
BtsI	GCAGTG	2 cut(s)	454, 666
BtsIMutI	CAGTG	8 cut(s)	187, 235, 454, 654, 666, 753, 1560, 1588
BveI	ACCTGC	1 cut(s)	1027
Cac8I	GCNNGC	2 cut(s)	531, 1221
Cfr13I	GGNCC	1 cut(s)	1292
Csp6I	GTAC	1 cut(s)	1550
CspCI	CAANNNNNGTGG	2 cut(s)	211, 246
CviQI	GTAC	1 cut(s)	1550
DdeI	CTNAG	7 cut(s)	443, 641, 925, 1134, 1497, 1593, 1712
DpnI	GATC	6 cut(s)	653, 1178, 1249, 1419, 1681, 1697
DpnII	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
DraI	TTTAAA	1 cut(s)	1032
DraIII	CACNNNGTG	1 cut(s)	344
Ecl136II	GAGCTC	1 cut(s)	1711
Eco130I	CCWWGG	2 cut(s)	119, 306
Eco24I	GRGCYC	1 cut(s)	1713
Eco31I	GGTCTC	1 cut(s)	928
Eco32I	GATATC	2 cut(s)	1378, 1603
Eco47I	GGWCC	1 cut(s)	1292
Eco53kI	GAGCTC	1 cut(s)	1711
EcoICRI	GAGCTC	1 cut(s)	1711
EcoO109I	RGGNCCY	1 cut(s)	1292
EcoRII	CCWGG	1 cut(s)	559
EcoRV	GATATC	2 cut(s)	1378, 1603
EcoT14I	CCWWGG	2 cut(s)	119, 306
EcoT22I	ATGCAT	1 cut(s)	490
EcoT38I	GRGCYC	1 cut(s)	1713
ErhI	CCWWGG	2 cut(s)	119, 306
Esp3I	CGTCTC	1 cut(s)	1130
FaqI	GGGAC	1 cut(s)	706
FbaI	TGATCA	1 cut(s)	651
FblI	GTMKAC	1 cut(s)	378
Fnu4HI	GCNGC	6 cut(s)	154, 659, 672, 1053, 1364, 1686
FokI	GGATG	4 cut(s)	102, 673, 1016, 1479
FriOI	GRGCYC	1 cut(s)	1713
Fsp4HI	GCNGC	6 cut(s)	154, 659, 672, 1053, 1364, 1686
FspBI	CTAG	3 cut(s)	1266, 1505, 1731
GluI	GCNGC	6 cut(s)	154, 659, 672, 1053, 1364, 1686
GsuI	CTGGAG	1 cut(s)	1245
HaeIII	GGCC	3 cut(s)	311, 318, 646
HapII	CCGG	2 cut(s)	690, 1371
HincII	GTYRAC	2 cut(s)	172, 1341
HindII	GTYRAC	2 cut(s)	172, 1341
HindIII	AAGCTT	1 cut(s)	793
HinfI	GANTC	4 cut(s)	144, 251, 302, 578
HpaI	GTTAAC	1 cut(s)	172
HpaII	CCGG	2 cut(s)	690, 1371
HphI	GGTGA	3 cut(s)	123, 356, 1286
Hpy166II	GTNNAC	6 cut(s)	172, 379, 1277, 1341, 1586, 1662
Hpy188I	TCNGA	4 cut(s)	1092, 1455, 1649, 1715
Hpy188III	TCNNGA	5 cut(s)	194, 1180, 1496, 1595, 1731
Hpy8I	GTNNAC	6 cut(s)	172, 379, 1277, 1341, 1586, 1662
HpyAV	CCTTC	5 cut(s)	39, 1106, 1305, 1636, 1698
HpyCH4III	ACNGT	3 cut(s)	230, 748, 1583
HpyCH4IV	ACGT	1 cut(s)	1614
HpyF10VI	GCNNNNNNNGC	3 cut(s)	364, 760, 1708
HpyF3I	CTNAG	7 cut(s)	443, 641, 925, 1134, 1497, 1593, 1712
HpySE526I	ACGT	1 cut(s)	1614
KpnI	GGTACC	1 cut(s)	1553
Ksp22I	TGATCA	1 cut(s)	651
KspAI	GTTAAC	1 cut(s)	172
Kzo9I	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
LmnI	GCTCC	6 cut(s)	751, 1049, 1060, 1213, 1465, 1708
Lsp1109I	GCAGC	6 cut(s)	165, 658, 670, 1064, 1350, 1697
LweI	GCATC	1 cut(s)	1501
MaeI	CTAG	3 cut(s)	1266, 1505, 1731
MaeII	ACGT	1 cut(s)	1614
MaeIII	GTNAC	6 cut(s)	182, 424, 946, 1284, 1310, 1516
MalI	GATC	6 cut(s)	653, 1178, 1249, 1419, 1681, 1697
MboI	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
MboII	GAAGA	6 cut(s)	811, 910, 1182, 1547, 1610, 1705
MfeI	CAATTG	4 cut(s)	549, 930, 1194, 1344
MflI	RGATCY	3 cut(s)	1247, 1679, 1695
MhlI	GDGCHC	2 cut(s)	1015, 1713
MluCI	AATT	8 cut(s)	370, 549, 733, 902, 930, 1194, 1299, 1344
MlyI	GAGTC	1 cut(s)	311
MmeI	TCCRAC	3 cut(s)	841, 1082, 1492
Mph1103I	ATGCAT	1 cut(s)	490
MseI	TTAA	8 cut(s)	62, 171, 462, 474, 912, 977, 986, 1031
MslI	CAYNNNNRTG	3 cut(s)	165, 326, 1581
MspI	CCGG	2 cut(s)	690, 1371
MspR9I	CCNGG	3 cut(s)	561, 691, 1372
MunI	CAATTG	4 cut(s)	549, 930, 1194, 1344
MvaI	CCWGG	1 cut(s)	561
MwoI	GCNNNNNNNGC	3 cut(s)	364, 760, 1708
NciI	CCSGG	2 cut(s)	691, 1372
NcoI	CCATGG	2 cut(s)	119, 306
NdeII	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
NlaIV	GGNNCC	2 cut(s)	1249, 1551
NmuCI	GTSAC	2 cut(s)	182, 946
NsiI	ATGCAT	1 cut(s)	490
NspI	RCATGY	2 cut(s)	492, 1219
PfeI	GAWTC	3 cut(s)	144, 251, 578
PfoI	TCCNGGA	2 cut(s)	559, 689
PkrI	GCNGC	6 cut(s)	155, 660, 673, 1054, 1365, 1687
PleI	GAGTC	1 cut(s)	310
PpsI	GAGTC	1 cut(s)	310
PpuMI	RGGWCCY	1 cut(s)	1292
Psp124BI	GAGCTC	1 cut(s)	1713
Psp5II	RGGWCCY	1 cut(s)	1292
Psp6I	CCWGG	1 cut(s)	559
PspGI	CCWGG	1 cut(s)	559
PspN4I	GGNNCC	2 cut(s)	1249, 1551
PspPI	GGNCC	1 cut(s)	1292
PspPPI	RGGWCCY	1 cut(s)	1292
PsrI	GAACNNNNNNTAC	2 cut(s)	1269, 1301
PstI	CTGCAG	4 cut(s)	1026, 1147, 1468, 1687
PsuI	RGATCY	3 cut(s)	1247, 1679, 1695
RsaI	GTAC	1 cut(s)	1551
RsaNI	GTAC	1 cut(s)	1550
RseI	CAYNNNNRTG	3 cut(s)	165, 326, 1581
SacI	GAGCTC	1 cut(s)	1713
SaqAI	TTAA	8 cut(s)	62, 171, 462, 474, 912, 977, 986, 1031
SatI	GCNGC	6 cut(s)	154, 659, 672, 1053, 1364, 1686
Sau3AI	GATC	6 cut(s)	651, 1176, 1247, 1417, 1679, 1695
Sau96I	GGNCC	1 cut(s)	1292
SchI	GAGTC	1 cut(s)	311
ScrFI	CCNGG	3 cut(s)	561, 691, 1372
SduI	GDGCHC	2 cut(s)	1015, 1713
SfaNI	GCATC	1 cut(s)	1501
SfcI	CTRYAG	7 cut(s)	148, 744, 1022, 1143, 1464, 1683, 1736
SinI	GGWCC	1 cut(s)	1292
SmiMI	CAYNNNNRTG	3 cut(s)	165, 326, 1581
SmlI	CTYRAG	1 cut(s)	49
SmoI	CTYRAG	1 cut(s)	49
Sse9I	AATT	8 cut(s)	370, 549, 733, 902, 930, 1194, 1299, 1344
SsiI	CCGC	1 cut(s)	763
SspI	AATATT	2 cut(s)	975, 1187
SspMI	CTAG	3 cut(s)	1266, 1505, 1731
SstI	GAGCTC	1 cut(s)	1713
StyD4I	CCNGG	3 cut(s)	559, 689, 1370
StyI	CCWWGG	2 cut(s)	119, 306
TaaI	ACNGT	3 cut(s)	230, 748, 1583
TaiI	ACGT	1 cut(s)	1617
TaqI	TCGA	2 cut(s)	300, 881
TasI	AATT	8 cut(s)	370, 549, 733, 902, 930, 1194, 1299, 1344
TfiI	GAWTC	3 cut(s)	144, 251, 578
Tru1I	TTAA	8 cut(s)	62, 171, 462, 474, 912, 977, 986, 1031
Tru9I	TTAA	8 cut(s)	62, 171, 462, 474, 912, 977, 986, 1031
TscAI	CASTG	8 cut(s)	187, 235, 454, 654, 673, 753, 1560, 1588
TseFI	GTSAC	2 cut(s)	182, 946
TseI	GCWGC	6 cut(s)	153, 658, 671, 1052, 1363, 1685
Tsp45I	GTSAC	2 cut(s)	182, 946
TspDTI	ATGAA	8 cut(s)	199, 449, 525, 675, 821, 911, 1082, 1548
TspGWI	ACGGA	1 cut(s)	1413
TspRI	CASTG	8 cut(s)	187, 235, 454, 654, 673, 753, 1560, 1588
VpaK11BI	GGWCC	1 cut(s)	1292
XapI	RAATTY	2 cut(s)	733, 902
XbaI	TCTAGA	1 cut(s)	1730
XceI	RCATGY	2 cut(s)	492, 1219
XcmI	CCANNNNNNNNNTGG	1 cut(s)	394
XmiI	GTMKAC	1 cut(s)	378
XspI	CTAG	3 cut(s)	1266, 1505, 1731
Zsp2I	ATGCAT	1 cut(s)	490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.