Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005432

Physical Location & Seq

Genomic Coordinates Reverse (-)

4859343 .. 4861058

Gene Structure 1716 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1050700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCCATGACTACCTTAACCAAGAAGTTCAACAACCATATCTCACAGAAACAGAGCACTCAGGATGGCACTCCGGATCACAAGTACCCATGGTGGTTTTCACCAGAAACCGGAATTTATTGCAGTAAACACCCAGAAATTACCCTCCCCACAAACCATTTTCTGGATGTTGTCACCTTCATCTTCTCACACCAGCATACCGGGGTTTCACCTGCCCTCATTGATTCCTCATCTGGGTCCTCACTTTCTTACTCCCAGCTCTACTCTTTTGTCAAATCATTTGCCTCCGGCCTCCGGCAAATGGGGATTTCACAAGGTGACGTTGTTCTGTTCCTTTTGCCGAATTCCATTTATTATCCCATTGTTTTCTTGGGAGTTTTGTACGCTGGTGCAATTGTCACGACGATGAATCCTCTCAGCAGTGTGGAGGAAATCAAGAAACAGGTTGGCGATTCGAAAGCGTGTCATGCCTTTACTGGACTTGAATATGTTGGTAAACTACAAGCATTGGGTGTTCCTGCAGTTTTGGTTCCGGAAAATGCAGTTTTGGATTCGAAGAATGAGGCTTTTTCGGTTTTTTATAAGCTTGTTAATGGTAATGTTGGTTTGGCTCCAAGACCAGTGATTAGGCAGCAAGACACCGCGGCGATAATGTACTCTTCCGGGACTACTGGTGCGAGCAAAGGTGTTGTGATCACACACAGGAATTTCATAGCTGCAGTGGAGCTTTTCGTGCGGTTTGAGGCTTCACAGTACGAGGGTTCGAGTTTGAGCGATGTGTACTTGGATGTTCTGCCGTTGTTTCATATTTATGGGTTGTTGCTCTTCGTGATGGGGTTGTTTTCTTTAGGATCAAGAATTGTTGTGATGAAGAAATTTGATGTCAGTGAGGTGGTGAAAGCAATTGAGAAGTACAAAGTTACACACTTTCCGGTTGTTCCGCCGATTTTAACAGCATTGACAGCAAAAGCTAAGGATGGTTTGGCCAAACAAAGCCTCAAGAGTTTGAAGCAGGTTTCATCCGGAGCAGCCCCTTTGAGCGCGAAATCGATAGAGGACTTTGTTCGGACTCTTCCTCATGTCGATTTGATTCAGGGTTATGGAATGACTGAGACCACGGCAGTAGGAACTCGCGGTTTCAACACCGAAAGCCTTAAAAAACCCTCTTCAATCGGGCTTTTAGCTCCAAACATGCAGGCCAAAGTGGTGGATTGGAAAACAGGATCCCCTCTGCCTCCCACAAGCAGCGGTGAGCTGTGGCTGCGCGGGCCAGGGATCATGAATGGATACTTGAACAATGCCGACGCAACAATGTCGACAATTGATGAAGAGGGTTGGCTGCACACAGGGGACATTGTGTACTTTGACGAAGACGGATACTTATACCTACAAGACCGGCTTAAAGACGCGATCAAATACAAGGGGTTTCAGATTGCACCGGCGGATTTAGAAGGCGTGCTGATCAACCATCCTGAGATACTTGATGTTGCAGTGGCAGGTGTGGTGGATGAGGAAAGTGGAGAGATACCAGTGGCATTTGTGGTGAGGAAGCACGGAAGTCGAATTTTGGAGGATAACGTGATGGATTTTGTGGCTGCACAAGTTGCTCCATACAAGAAGGTGAGGAAGGTGGTTTTCACAGATTCGATTCCAAAGTCTGCAGCTGGGAAGATCCTAAGAAGGGAGCTTAGGAAGGTCCTGACCTCTAGACTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006732 GO:0006733 GO:0006743 GO:0006744 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0042180 GO:0042181 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

572

Amino Acids

62.38

Weight (kDa)

7.73

Isoelectric Point (pI)

40.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	69 - 431	2.7e-91	AMP-binding enzyme
AMP-binding_C	PF13193	483 - 557	4.7e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19010
fragaria_vesca	FvH4_5g23550
malus_domestica	MD14G1050700.v1.1
prunus_persica	Prupe.7G085200_v2.0.a1 Prupe.8G160600_v2.0.a1
pyrus_communis	pycom12g04570 pycom14g04200
rosa_chinensis	RchiOBHm_Chr3g0478721 RchiOBHm_Chr7g0212861
rosa_laevigata	RLG00000002864 RLG00000023630
rosa_multiflora	Rmu_sc0000394.1_g000050 Rmu_sc0001519.1_g000013
rosa_roxburghii	Rroxscaffold_3G00246500 Rroxscaffold_6G00403160
rosa_rugosa	Rorug03G0167300 Rorug03G0167400 Rorug07G0137100
rosa_samantha	Rh3AG219500 Rh3BG251200 Rh3CG248100 Rh3CG248200 Rh3DG244500 Rh7BG264300 Rh7CG287700 Rh7DG276800
rosa_wichuraiana	Rw3G019720 Rw7G023220 Rw7G023340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	582
AarI	CACCTGC	2 cut(s)	220, 1487
Acc36I	ACCTGC	3 cut(s)	220, 1003, 1487
AccB7I	CCANNNNNTGG	1 cut(s)	163
AccI	GTMKAC	1 cut(s)	1316
AccII	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
AccIII	TCCGGA	3 cut(s)	73, 532, 1022
AciI	CCGC	8 cut(s)	642, 644, 736, 941, 1134, 1248, 1266, 1442
AclWI	GGATC	6 cut(s)	84, 859, 1218, 1231, 1283, 1666
AcoI	YGGCCR	2 cut(s)	3, 984
AcsI	RAATTY	5 cut(s)	114, 343, 706, 875, 1563
AdeI	CACNNNGTG	1 cut(s)	317
AfaI	GTAC	7 cut(s)	86, 383, 656, 755, 782, 914, 1361
AfiI	CCNNNNNNNGG	6 cut(s)	110, 163, 234, 294, 301, 1681
AgsI	TTSAA	6 cut(s)	31, 485, 1009, 1141, 1170, 1294
AjnI	CCWGG	1 cut(s)	1270
AluBI	AGCT	9 cut(s)	259, 586, 716, 727, 971, 1184, 1255, 1664, 1687
AluI	AGCT	9 cut(s)	259, 586, 716, 727, 971, 1184, 1255, 1664, 1687
Alw21I	GWGCWC	1 cut(s)	59
Alw26I	GTCTC	1 cut(s)	1106
AlwI	GGATC	6 cut(s)	84, 859, 1218, 1231, 1283, 1666
Aor13HI	TCCGGA	3 cut(s)	73, 532, 1022
AoxI	GGCC	5 cut(s)	3, 289, 984, 1197, 1268
ApeKI	GCWGC	8 cut(s)	631, 716, 1028, 1245, 1261, 1339, 1595, 1661
ApoI	RAATTY	5 cut(s)	114, 343, 706, 875, 1563
AspLEI	GCGC	2 cut(s)	1043, 1266
AspS9I	GGNCC	3 cut(s)	237, 1268, 1696
AsuC2I	CCSGG	2 cut(s)	202, 664
AsuHPI	GGTGA	8 cut(s)	93, 166, 201, 329, 907, 1262, 1555, 1633
AsuII	TTCGAA	2 cut(s)	455, 554
AvaII	GGWCC	2 cut(s)	237, 1696
BalI	TGGCCA	2 cut(s)	5, 986
BamHI	GGATCC	1 cut(s)	1223
BbsI	GAAGAC	1 cut(s)	1377
Bbv12I	GWGCWC	1 cut(s)	59
BbvI	GCAGC	8 cut(s)	643, 703, 1040, 1248, 1257, 1326, 1582, 1673
BccI	CCATC	5 cut(s)	59, 826, 971, 1476, 1576
BceAI	ACGGC	2 cut(s)	781, 1134
BcgI	CGANNNNNNTGC	4 cut(s)	1296, 1330, 1541, 1575
BciT130I	CCWGG	1 cut(s)	1272
BciVI	GTATCC	2 cut(s)	1280, 1370
BclI	TGATCA	2 cut(s)	693, 1461
BcnI	CCSGG	2 cut(s)	202, 664
BcoDI	GTCTC	1 cut(s)	1106
BfaI	CTAG	1 cut(s)	1707
BfmI	CTRYAG	3 cut(s)	519, 717, 1659
BfuAI	ACCTGC	3 cut(s)	220, 1003, 1487
BfuI	GTATCC	2 cut(s)	1280, 1370
BisI	GCNGC	9 cut(s)	632, 645, 717, 1029, 1246, 1262, 1340, 1596, 1662
BlsI	GCNGC	9 cut(s)	633, 646, 718, 1030, 1247, 1263, 1341, 1597, 1663
Bme1390I	CCNGG	3 cut(s)	202, 664, 1272
Bme18I	GGWCC	2 cut(s)	237, 1696
BmgT120I	GGNCC	3 cut(s)	237, 1268, 1696
BmiI	GGNNCC	4 cut(s)	238, 531, 612, 1225
BmrFI	CCNGG	3 cut(s)	202, 664, 1272
BpiI	GAAGAC	1 cut(s)	1377
Bpu10I	CCTNAGC	2 cut(s)	972, 1688
Bpu14I	TTCGAA	2 cut(s)	455, 554
BpuEI	CTTGAG	1 cut(s)	983
BpuMI	CCSGG	2 cut(s)	202, 664
Bsa29I	ATCGAT	1 cut(s)	1049
BsaI	GGTCTC	1 cut(s)	1106
BsaJI	CCNNGG	5 cut(s)	89, 201, 642, 1116, 1271
BsaWI	WCCGGW	5 cut(s)	73, 110, 532, 931, 1022
Bsc4I	CCNNNNNNNGG	6 cut(s)	110, 163, 234, 294, 301, 1681
Bse118I	RCCGGY	2 cut(s)	1395, 1438
Bse1I	ACTGG	4 cut(s)	481, 620, 676, 1529
BseAI	TCCGGA	3 cut(s)	73, 532, 1022
BseBI	CCWGG	1 cut(s)	1272
BseCI	ATCGAT	1 cut(s)	1049
BseDI	CCNNGG	5 cut(s)	89, 201, 642, 1116, 1271
BseGI	GGATG	7 cut(s)	70, 172, 793, 982, 1019, 1468, 1513
BseLI	CCNNNNNNNGG	6 cut(s)	110, 163, 234, 294, 301, 1681
BseMII	CTCAG	4 cut(s)	74, 430, 1101, 1464
BseNI	ACTGG	4 cut(s)	481, 620, 676, 1529
BseXI	GCAGC	8 cut(s)	643, 703, 1040, 1248, 1257, 1326, 1582, 1673
BseYI	CCCAGC	2 cut(s)	255, 1664
BsgI	GTGCAG	2 cut(s)	1325, 1581
Bsh1236I	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
BshFI	GGCC	5 cut(s)	5, 291, 986, 1199, 1270
BshVI	ATCGAT	1 cut(s)	1049
BsiHKAI	GWGCWC	1 cut(s)	59
BslFI	GGGAC	2 cut(s)	679, 1364
BslI	CCNNNNNNNGG	6 cut(s)	110, 163, 234, 294, 301, 1681
BsmAI	GTCTC	1 cut(s)	1106
BsmFI	GGGAC	2 cut(s)	679, 1364
BsnI	GGCC	5 cut(s)	5, 291, 986, 1199, 1270
Bso31I	GGTCTC	1 cut(s)	1106
Bsp119I	TTCGAA	2 cut(s)	455, 554
Bsp1286I	GDGCHC	1 cut(s)	59
Bsp13I	TCCGGA	3 cut(s)	73, 532, 1022
Bsp143I	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
Bsp19I	CCATGG	1 cut(s)	89
BspACI	CCGC	8 cut(s)	642, 644, 736, 941, 1134, 1248, 1266, 1442
BspANI	GGCC	5 cut(s)	5, 291, 986, 1199, 1270
BspCNI	CTCAG	4 cut(s)	73, 429, 1102, 1465
BspDI	ATCGAT	1 cut(s)	1049
BspEI	TCCGGA	3 cut(s)	73, 532, 1022
BspFNI	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
BspHI	TCATGA	1 cut(s)	1278
BspLI	GGNNCC	4 cut(s)	238, 531, 612, 1225
BspMAI	CTGCAG	3 cut(s)	523, 721, 1663
BspMI	ACCTGC	3 cut(s)	220, 1003, 1487
BspPI	GGATC	6 cut(s)	84, 859, 1218, 1231, 1283, 1666
BspQI	GCTCTTC	1 cut(s)	830
BspT104I	TTCGAA	2 cut(s)	455, 554
BspTNI	GGTCTC	1 cut(s)	1106
BsrFI	RCCGGY	2 cut(s)	1395, 1438
BsrI	ACTGG	4 cut(s)	481, 620, 676, 1529
BssAI	RCCGGY	2 cut(s)	1395, 1438
BssECI	CCNNGG	5 cut(s)	89, 201, 642, 1116, 1271
BssMI	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
BssT1I	CCWWGG	1 cut(s)	89
Bst2UI	CCWGG	1 cut(s)	1272
Bst4CI	ACNGT	1 cut(s)	753
Bst6I	CTCTTC	5 cut(s)	664, 830, 1077, 1171, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	1604
BstBI	TTCGAA	2 cut(s)	455, 554
BstC8I	GCNNGC	4 cut(s)	679, 1197, 1268, 1457
BstDEI	CTNAG	7 cut(s)	60, 416, 972, 1110, 1473, 1676, 1688
BstDSI	CCRYGG	3 cut(s)	89, 642, 1116
BstF5I	GGATG	7 cut(s)	70, 172, 793, 982, 1019, 1468, 1513
BstFNI	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
BstHHI	GCGC	2 cut(s)	1043, 1266
BstKTI	GATC	8 cut(s)	79, 696, 854, 1226, 1278, 1413, 1464, 1674
BstMAI	GTCTC	1 cut(s)	1106
BstMBI	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
BstMWI	GCNNNNNNNGC	6 cut(s)	467, 733, 962, 1261, 1267, 1604
BstNI	CCWGG	1 cut(s)	1272
BstNSI	RCATGY	1 cut(s)	1195
BstSCI	CCNGG	3 cut(s)	200, 662, 1270
BstSFI	CTRYAG	3 cut(s)	519, 717, 1659
BstUI	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
BstV1I	GCAGC	8 cut(s)	643, 703, 1040, 1248, 1257, 1326, 1582, 1673
BstV2I	GAAGAC	1 cut(s)	1377
BstX2I	RGATCY	2 cut(s)	1223, 1671
BstXI	CCANNNNNNTGG	1 cut(s)	1207
BstYI	RGATCY	2 cut(s)	1223, 1671
Bsu15I	ATCGAT	1 cut(s)	1049
BsuI	GTATCC	2 cut(s)	1280, 1370
BsuRI	GGCC	5 cut(s)	5, 291, 986, 1199, 1270
BsuTUI	ATCGAT	1 cut(s)	1049
BtgI	CCRYGG	3 cut(s)	89, 642, 1116
BtgZI	GCGATG	1 cut(s)	789
BtsCI	GGATG	7 cut(s)	70, 172, 793, 982, 1019, 1468, 1513
BtsI	GCAGTG	3 cut(s)	427, 726, 1497
BtsIMutI	CAGTG	6 cut(s)	427, 627, 726, 892, 1497, 1536
BveI	ACCTGC	3 cut(s)	220, 1003, 1487
Cac8I	GCNNGC	4 cut(s)	679, 1197, 1268, 1457
CciI	TCATGA	1 cut(s)	1278
CfoI	GCGC	2 cut(s)	1043, 1266
Cfr10I	RCCGGY	2 cut(s)	1395, 1438
Cfr13I	GGNCC	3 cut(s)	237, 1268, 1696
Cfr42I	CCGCGG	1 cut(s)	645
ClaI	ATCGAT	1 cut(s)	1049
CseI	GACGC	2 cut(s)	1313, 1415
Csp6I	GTAC	7 cut(s)	85, 382, 655, 754, 781, 913, 1360
CviAII	CATG	6 cut(s)	7, 90, 467, 1079, 1192, 1279
CviQI	GTAC	7 cut(s)	85, 382, 655, 754, 781, 913, 1360
DdeI	CTNAG	7 cut(s)	60, 416, 972, 1110, 1473, 1676, 1688
DpnI	GATC	8 cut(s)	78, 695, 853, 1225, 1277, 1412, 1463, 1673
DpnII	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
DraIII	CACNNNGTG	1 cut(s)	317
EaeI	YGGCCR	2 cut(s)	3, 984
Eam1104I	CTCTTC	5 cut(s)	664, 830, 1077, 1171, 1323
EarI	CTCTTC	5 cut(s)	664, 830, 1077, 1171, 1323
EciI	GGCGGA	2 cut(s)	930, 1457
Eco130I	CCWWGG	1 cut(s)	89
Eco31I	GGTCTC	1 cut(s)	1106
Eco47I	GGWCC	2 cut(s)	237, 1696
EcoO109I	RGGNCCY	2 cut(s)	237, 1696
EcoRI	GAATTC	1 cut(s)	343
EcoRII	CCWGG	1 cut(s)	1270
EcoT14I	CCWWGG	1 cut(s)	89
ErhI	CCWWGG	1 cut(s)	89
FaeI	CATG	6 cut(s)	10, 93, 470, 1082, 1195, 1282
FalI	AAGNNNNNCTT	2 cut(s)	1383, 1415
FaqI	GGGAC	2 cut(s)	679, 1364
FatI	CATG	6 cut(s)	6, 89, 466, 1078, 1191, 1278
FauI	CCCGC	1 cut(s)	1259
FbaI	TGATCA	2 cut(s)	693, 1461
FblI	GTMKAC	1 cut(s)	1316
Fnu4HI	GCNGC	9 cut(s)	632, 645, 717, 1029, 1246, 1262, 1340, 1596, 1662
FokI	GGATG	7 cut(s)	77, 179, 800, 989, 1006, 1455, 1520
Fsp4HI	GCNGC	9 cut(s)	632, 645, 717, 1029, 1246, 1262, 1340, 1596, 1662
FspBI	CTAG	1 cut(s)	1707
GlaI	GCGC	2 cut(s)	1042, 1265
GluI	GCNGC	9 cut(s)	632, 645, 717, 1029, 1246, 1262, 1340, 1596, 1662
GsaI	CCCAGC	2 cut(s)	259, 1668
HaeIII	GGCC	5 cut(s)	5, 291, 986, 1199, 1270
HgaI	GACGC	2 cut(s)	1313, 1415
HhaI	GCGC	2 cut(s)	1043, 1266
Hin1II	CATG	6 cut(s)	10, 93, 470, 1082, 1195, 1282
Hin6I	GCGC	2 cut(s)	1041, 1264
HinP1I	GCGC	2 cut(s)	1041, 1264
HincII	GTYRAC	1 cut(s)	1317
HindII	GTYRAC	1 cut(s)	1317
HindIII	AAGCTT	1 cut(s)	584
HinfI	GANTC	9 cut(s)	224, 409, 452, 551, 1069, 1090, 1643, 1648, 1710
HphI	GGTGA	8 cut(s)	93, 166, 201, 329, 907, 1262, 1555, 1633
Hpy166II	GTNNAC	5 cut(s)	128, 497, 781, 1317, 1360
Hpy188I	TCNGA	2 cut(s)	1068, 1431
Hpy8I	GTNNAC	5 cut(s)	128, 497, 781, 1317, 1360
Hpy99I	CGWCG	2 cut(s)	406, 1307
HpyAV	CCTTC	6 cut(s)	187, 1445, 1612, 1621, 1674, 1687
HpyCH4III	ACNGT	1 cut(s)	753
HpyCH4IV	ACGT	2 cut(s)	321, 1578
HpyF10VI	GCNNNNNNNGC	6 cut(s)	467, 733, 962, 1261, 1267, 1604
HpyF3I	CTNAG	7 cut(s)	60, 416, 972, 1110, 1473, 1676, 1688
HpySE526I	ACGT	2 cut(s)	321, 1578
Hsp92II	CATG	6 cut(s)	10, 93, 470, 1082, 1195, 1282
HspAI	GCGC	2 cut(s)	1041, 1264
Kpn2I	TCCGGA	3 cut(s)	73, 532, 1022
Ksp22I	TGATCA	2 cut(s)	693, 1461
KspI	CCGCGG	1 cut(s)	645
Kzo9I	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
LguI	GCTCTTC	1 cut(s)	830
LmnI	GCTCC	6 cut(s)	616, 724, 1025, 1189, 1612, 1684
Lsp1109I	GCAGC	8 cut(s)	643, 703, 1040, 1248, 1257, 1326, 1582, 1673
MaeI	CTAG	1 cut(s)	1707
MaeII	ACGT	2 cut(s)	321, 1578
MaeIII	GTNAC	4 cut(s)	172, 317, 397, 919
MalI	GATC	8 cut(s)	78, 695, 853, 1225, 1277, 1412, 1463, 1673
MboI	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
MfeI	CAATTG	3 cut(s)	393, 903, 1320
MflI	RGATCY	2 cut(s)	1223, 1671
MhlI	GDGCHC	1 cut(s)	59
MlsI	TGGCCA	2 cut(s)	5, 986
MluNI	TGGCCA	2 cut(s)	5, 986
MlyI	GAGTC	2 cut(s)	1063, 1704
Mox20I	TGGCCA	2 cut(s)	5, 986
MroI	TCCGGA	3 cut(s)	73, 532, 1022
MscI	TGGCCA	2 cut(s)	5, 986
MseI	TTAA	5 cut(s)	17, 591, 950, 1155, 1401
MslI	CAYNNNNRTG	2 cut(s)	404, 810
Msp20I	TGGCCA	2 cut(s)	5, 986
MspA1I	CMGCKG	3 cut(s)	644, 1248, 1664
MspR9I	CCNGG	3 cut(s)	202, 664, 1272
MunI	CAATTG	3 cut(s)	393, 903, 1320
MvaI	CCWGG	1 cut(s)	1272
MvnI	CGCG	5 cut(s)	644, 1043, 1134, 1266, 1409
MwoI	GCNNNNNNNGC	6 cut(s)	467, 733, 962, 1261, 1267, 1604
NciI	CCSGG	2 cut(s)	202, 664
NcoI	CCATGG	1 cut(s)	89
NdeII	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
NlaIII	CATG	6 cut(s)	10, 93, 470, 1082, 1195, 1282
NlaIV	GGNNCC	4 cut(s)	238, 531, 612, 1225
NmuCI	GTSAC	3 cut(s)	172, 317, 397
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	2 cut(s)	455, 554
PagI	TCATGA	1 cut(s)	1278
PaqCI	CACCTGC	2 cut(s)	220, 1487
PciSI	GCTCTTC	1 cut(s)	830
PfeI	GAWTC	7 cut(s)	224, 409, 452, 551, 1090, 1643, 1648
PflMI	CCANNNNNTGG	1 cut(s)	163
PfoI	TCCNGGA	1 cut(s)	662
PkrI	GCNGC	9 cut(s)	633, 646, 718, 1030, 1247, 1263, 1341, 1597, 1663
PleI	GAGTC	2 cut(s)	1063, 1704
PpsI	GAGTC	2 cut(s)	1063, 1704
PpuMI	RGGWCCY	2 cut(s)	237, 1696
PsiI	TTATAA	1 cut(s)	582
Psp5II	RGGWCCY	2 cut(s)	237, 1696
Psp6I	CCWGG	1 cut(s)	1270
PspFI	CCCAGC	2 cut(s)	255, 1664
PspGI	CCWGG	1 cut(s)	1270
PspN4I	GGNNCC	4 cut(s)	238, 531, 612, 1225
PspPI	GGNCC	3 cut(s)	237, 1268, 1696
PspPPI	RGGWCCY	2 cut(s)	237, 1696
PstI	CTGCAG	3 cut(s)	523, 721, 1663
PsuI	RGATCY	2 cut(s)	1223, 1671
PvuII	CAGCTG	1 cut(s)	1664
RsaI	GTAC	7 cut(s)	86, 383, 656, 755, 782, 914, 1361
RsaNI	GTAC	7 cut(s)	85, 382, 655, 754, 781, 913, 1360
RseI	CAYNNNNRTG	2 cut(s)	404, 810
SacII	CCGCGG	1 cut(s)	645
SalI	GTCGAC	1 cut(s)	1315
SapI	GCTCTTC	1 cut(s)	830
SaqAI	TTAA	5 cut(s)	17, 591, 950, 1155, 1401
SatI	GCNGC	9 cut(s)	632, 645, 717, 1029, 1246, 1262, 1340, 1596, 1662
Sau3AI	GATC	8 cut(s)	76, 693, 851, 1223, 1275, 1410, 1461, 1671
Sau96I	GGNCC	3 cut(s)	237, 1268, 1696
SchI	GAGTC	2 cut(s)	1063, 1704
ScrFI	CCNGG	3 cut(s)	202, 664, 1272
SduI	GDGCHC	1 cut(s)	59
SfcI	CTRYAG	3 cut(s)	519, 717, 1659
Sfr303I	CCGCGG	1 cut(s)	645
SfuI	TTCGAA	2 cut(s)	455, 554
SgrAI	CRCCGGYG	1 cut(s)	1438
SgrBI	CCGCGG	1 cut(s)	645
SinI	GGWCC	2 cut(s)	237, 1696
SmiMI	CAYNNNNRTG	2 cut(s)	404, 810
SmlI	CTYRAG	1 cut(s)	998
SmoI	CTYRAG	1 cut(s)	998
SsiI	CCGC	8 cut(s)	642, 644, 736, 941, 1134, 1248, 1266, 1442
SspMI	CTAG	1 cut(s)	1707
StyD4I	CCNGG	3 cut(s)	200, 662, 1270
StyI	CCWWGG	1 cut(s)	89
TaaI	ACNGT	1 cut(s)	753
TaiI	ACGT	2 cut(s)	324, 1581
TaqI	TCGA	8 cut(s)	455, 554, 764, 1049, 1083, 1316, 1561, 1646
TatI	WGTACW	4 cut(s)	654, 780, 912, 1359
TauI	GCSGC	1 cut(s)	647
TfiI	GAWTC	7 cut(s)	224, 409, 452, 551, 1090, 1643, 1648
Tru1I	TTAA	5 cut(s)	17, 591, 950, 1155, 1401
Tru9I	TTAA	5 cut(s)	17, 591, 950, 1155, 1401
TscAI	CASTG	6 cut(s)	427, 627, 726, 892, 1497, 1536
TseFI	GTSAC	3 cut(s)	172, 317, 397
TseI	GCWGC	8 cut(s)	631, 716, 1028, 1245, 1261, 1339, 1595, 1661
Tsp45I	GTSAC	3 cut(s)	172, 317, 397
TspDTI	ATGAA	8 cut(s)	169, 422, 700, 794, 884, 1008, 1295, 1341
TspGWI	ACGGA	2 cut(s)	1389, 1569
TspRI	CASTG	6 cut(s)	427, 627, 726, 892, 1497, 1536
Van91I	CCANNNNNTGG	1 cut(s)	163
VpaK11BI	GGWCC	2 cut(s)	237, 1696
XapI	RAATTY	5 cut(s)	114, 343, 706, 875, 1563
XbaI	TCTAGA	1 cut(s)	1706
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	367
XmiI	GTMKAC	1 cut(s)	1316
XspI	CTAG	1 cut(s)	1707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.