FvH4_5g27010

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008140
Physical Location & Seq
Reverse (-)
18318090 .. 18318797
708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27010.t1

Sequence Viewer

Length: 708 bp
ATGCACAGAGCTGCTAGACGGATAAAGGTAATCAGTTTATTCAGAAAGTTGAATGATGGAACAAAGAAAAACAACTCTCTGAGGGGAAAATGTAAAGACAGGTCAAGTGAAGAAACTAATCATACCAGCCAGGACAAAGTATTTCACTTGGGCGTAAATTCTGTTGAATTGAATAGCAGAACTCACTGGAGCCTCACTACAGATAATTGCTACTTGAACCAAAATCTCAGCAACAAGTACCAACCAAGGCTTCTCAAGGACATTGCAGGCCATGAAATGGTTGTGAAGACCATAACTAATGCAATTCAGCAGAAAAAGATCGCACCACTTTATCTTTTCTATGGTGCAAGAGGCTTTGGGAAAACATCTACTGCTAGAATTTTAGCCTTGGCACTGAACTGTGAATCCAGTTTGAATATCAAACCGTGCTGGAAATGCCAAGGATGCAACAAAAGCCTTTCTATTATGGAATTGTGTTCCGGAAGCACAGTTGCTGTTTTCAAATGGATCAGGAAACTCCTCCGAAGTATGTTATCTTTGCAAGCAACACCAGGATTCAAGGTATTTGTCCTTGAAGAGTGTGAATCAATTAGTACAGAAGCGTGGGACGAACTTTCAGGGATAGCAAGTGGAGCTTACAATGCTGGTGTATTCATCATTATCATCGAGATGTCAGAAGTTCTTCTTTCCCAAACTCAGAGACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

236

Amino Acids

26.4

Weight (kDa)

9.51

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 91 - 202 3.1e-11 DNA polymerase III, delta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 479
AclWI GGATC 1 cut(s) 515
AcsI RAATTY 2 cut(s) 157, 378
AfaI GTAC 2 cut(s) 239, 595
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 8 cut(s) 52, 167, 172, 217, 415, 502, 559, 575
AjnI CCWGG 2 cut(s) 129, 550
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 2 cut(s) 11, 635
AluI AGCT 2 cut(s) 11, 635
Alw26I GTCTC 1 cut(s) 694
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 479
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 157, 378
Asp700I GAANNNNTTC 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 293
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 2 cut(s) 131, 552
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 2 cut(s) 15, 375
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 2 cut(s) 131, 552
BmiI GGNNCC 1 cut(s) 191
BmrFI CCNGG 2 cut(s) 131, 552
BmsI GCATC 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 245, 387, 439
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 191, 408
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 2 cut(s) 131, 552
BseDI CCNNGG 3 cut(s) 245, 387, 439
BseGI GGATG 1 cut(s) 449
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 71, 241
BseNI ACTGG 2 cut(s) 191, 408
BseRI GAGGAG 1 cut(s) 509
BshFI GGCC 1 cut(s) 270
BsiSI CCGG 1 cut(s) 480
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 1 cut(s) 694
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 1 cut(s) 270
Bsp13I TCCGGA 1 cut(s) 479
Bsp143I GATC 2 cut(s) 318, 507
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 2 cut(s) 72, 240
BspEI TCCGGA 1 cut(s) 479
BspLI GGNNCC 1 cut(s) 191
BspPI GGATC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 191, 408
BssECI CCNNGG 3 cut(s) 245, 387, 439
BssMI GATC 2 cut(s) 318, 507
BssT1I CCWWGG 3 cut(s) 245, 387, 439
Bst2UI CCWGG 2 cut(s) 131, 552
Bst4CI ACNGT 3 cut(s) 401, 426, 490
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 268, 543
BstDEI CTNAG 3 cut(s) 80, 227, 696
BstF5I GGATG 1 cut(s) 449
BstKTI GATC 2 cut(s) 321, 510
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 2 cut(s) 318, 507
BstMWI GCNNNNNNNGC 5 cut(s) 435, 444, 453, 632, 641
BstNI CCWGG 2 cut(s) 131, 552
BstSCI CCNGG 2 cut(s) 129, 550
BstSFI CTRYAG 1 cut(s) 198
BstV2I GAAGAC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 449
BtsIMutI CAGTG 3 cut(s) 184, 392, 703
Cac8I GCNNGC 2 cut(s) 268, 543
CaiI CAGNNNCTG 1 cut(s) 494
Csp6I GTAC 2 cut(s) 238, 594
CviAII CATG 1 cut(s) 272
CviJI RGCY 9 cut(s) 11, 129, 192, 250, 270, 354, 386, 456, 635
CviKI_1 RGCY 9 cut(s) 11, 129, 192, 250, 270, 354, 386, 456, 635
CviQI GTAC 2 cut(s) 238, 594
DdeI CTNAG 3 cut(s) 80, 227, 696
DpnI GATC 2 cut(s) 320, 509
DpnII GATC 2 cut(s) 318, 507
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 3 cut(s) 245, 387, 439
EcoRII CCWGG 2 cut(s) 129, 550
EcoT14I CCWWGG 3 cut(s) 245, 387, 439
ErhI CCWWGG 3 cut(s) 245, 387, 439
FaeI CATG 1 cut(s) 275
FaiI YATR 6 cut(s) 123, 273, 293, 342, 467, 530
FalI AAGNNNNNCTT 4 cut(s) 619, 651, 669, 701
FaqI GGGAC 1 cut(s) 620
FatI CATG 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 456
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 15, 375
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 1 cut(s) 270
HapII CCGG 1 cut(s) 480
Hin1II CATG 1 cut(s) 275
HinfI GANTC 3 cut(s) 404, 555, 584
HpaII CCGG 1 cut(s) 480
Hpy188I TCNGA 5 cut(s) 44, 81, 524, 676, 699
Hpy188III TCNNGA 3 cut(s) 480, 511, 667
HpyCH4III ACNGT 3 cut(s) 401, 426, 490
HpyCH4V TGCA 6 cut(s) 4, 266, 302, 347, 447, 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 435, 444, 453, 632, 641
HpyF3I CTNAG 3 cut(s) 80, 227, 696
Hsp92II CATG 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 479
Kzo9I GATC 2 cut(s) 318, 507
LmnI GCTCC 2 cut(s) 189, 632
LweI GCATC 1 cut(s) 434
MaeI CTAG 2 cut(s) 15, 375
MalI GATC 2 cut(s) 320, 509
MboI GATC 2 cut(s) 318, 507
MboII GAAGA 4 cut(s) 122, 298, 587, 674
MluCI AATT 7 cut(s) 157, 167, 205, 303, 378, 470, 588
MnlI CCTC 4 cut(s) 75, 203, 344, 530
MroI TCCGGA 1 cut(s) 479
MroXI GAANNNNTTC 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 668
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 2 cut(s) 131, 552
MvaI CCWGG 2 cut(s) 131, 552
MwoI GCNNNNNNNGC 5 cut(s) 435, 444, 453, 632, 641
NdeII GATC 2 cut(s) 318, 507
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 3 cut(s) 404, 555, 584
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 1 cut(s) 13
Psp6I CCWGG 2 cut(s) 129, 550
PspGI CCWGG 2 cut(s) 129, 550
PspN4I GGNNCC 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 239, 595
RsaNI GTAC 2 cut(s) 238, 594
RseI CAYNNNNRTG 1 cut(s) 668
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 318, 507
ScrFI CCNGG 2 cut(s) 131, 552
SetI ASST 5 cut(s) 13, 30, 104, 564, 637
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 7 cut(s) 157, 167, 205, 303, 378, 470, 588
SspMI CTAG 2 cut(s) 15, 375
StyD4I CCNGG 2 cut(s) 129, 550
StyI CCWWGG 3 cut(s) 245, 387, 439
TaaI ACNGT 3 cut(s) 401, 426, 490
TaqI TCGA 1 cut(s) 666
TasI AATT 7 cut(s) 157, 167, 205, 303, 378, 470, 588
TatI WGTACW 1 cut(s) 593
TfiI GAWTC 3 cut(s) 404, 555, 584
TscAI CASTG 2 cut(s) 191, 399
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 288, 643
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 2 cut(s) 191, 399
Van91I CCANNNNNTGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 157, 378
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 2 cut(s) 15, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.