Rh7AG314800

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008140
Physical Location & Seq
Reverse (-)
35349313 .. 35350294
982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG314800.1

Sequence Viewer

Length: 573 bp
ATGGCACTTTCCGGAGACACCATGCAAACCCTGAGATGTGCAAGGCAGCTGAGTGGAACTGGTATTGAACCACAAATAGATGCTTCCAGCAAAAGATCTTCCGGACCTGCCCTATCCCCAGGTACGTTCAAGTGCATCATCCTCAGACATGCAATTTCTATCTGGGAACAAAAATCAGTTTCATTAATTGAGTTGAAGTTGGCACAACTTATACCAGTTTTGTCTGCAGTAGCAGATTACAAGTCCAATGCAGAAATGGAGTTGCATAGCCTAACCCACAGCAGGTACTTGGTGAACCACATTGCCATCAGCAACACTGCTGAGCGACAAGAGATGGACAATACGAAACAAAAGTCTACCATCAAAAGCATTACGAAACAGACAAATTGCAGAGACAAGGCAGTTGATGTAGAGACACACTTTACTTGCATCAGTGAAATGGAAGAAATTTGGCTAAACATGTTGGGCAGGATGCATAGTTCATATTTGAAAGACTTTTTGCGCCACAAAGTGAAGCTTGCATCATTAACTATAACTAGTGGTAAAGCACTTTTACTCTACACTAGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

190

Amino Acids

21.21

Weight (kDa)

9.06

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaA_N-like_STI PF23007 145 - 183 2.5e-07 STICHEL, DnaA_N-like alpha-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 115, 273
AccI GTMKAC 1 cut(s) 356
AccIII TCCGGA 2 cut(s) 11, 101
AcsI RAATTY 1 cut(s) 447
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 2 cut(s) 124, 287
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 4 cut(s) 68, 130, 196, 490
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 118
AloI GAACNNNNNNTCC 2 cut(s) 463, 495
AluBI AGCT 2 cut(s) 49, 517
AluI AGCT 2 cut(s) 49, 517
Alw26I GTCTC 3 cut(s) 9, 387, 407
Aor13HI TCCGGA 2 cut(s) 11, 101
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 447
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AseI ATTAAT 1 cut(s) 185
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 304
AvaII GGWCC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 58
BccI CCATC 3 cut(s) 314, 328, 368
BciT130I CCWGG 1 cut(s) 120
BcoDI GTCTC 3 cut(s) 9, 387, 407
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 2 cut(s) 537, 564
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 2 cut(s) 115, 273
BglII AGATCT 1 cut(s) 95
BisI GCNGC 1 cut(s) 47
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 5 cut(s) 70, 144, 438, 462, 530
Bpu1102I GCTNAGC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 2 cut(s) 11, 101
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 64, 215
Bse3DI GCAATG 1 cut(s) 300
BseAI TCCGGA 2 cut(s) 11, 101
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 138, 477
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 4 cut(s) 23, 41, 157, 312
BseNI ACTGG 2 cut(s) 64, 215
BseXI GCAGC 1 cut(s) 58
BsiSI CCGG 2 cut(s) 12, 102
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 9, 387, 407
Bsp13I TCCGGA 2 cut(s) 11, 101
Bsp143I GATC 1 cut(s) 95
Bsp1720I GCTNAGC 1 cut(s) 321
BspCNI CTCAG 4 cut(s) 24, 42, 156, 313
BspEI TCCGGA 2 cut(s) 11, 101
BspMAI CTGCAG 1 cut(s) 229
BspMI ACCTGC 2 cut(s) 115, 273
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 2 cut(s) 64, 215
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 4 cut(s) 32, 50, 143, 321
BstF5I GGATG 2 cut(s) 138, 477
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 3 cut(s) 9, 387, 407
BstMBI GATC 1 cut(s) 95
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 152, 463
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BtsCI GGATG 2 cut(s) 138, 477
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 439
BveI ACCTGC 2 cut(s) 115, 273
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 123, 286
CviAII CATG 3 cut(s) 22, 149, 460
CviJI RGCY 4 cut(s) 49, 270, 454, 517
CviKI_1 RGCY 4 cut(s) 49, 270, 454, 517
CviQI GTAC 2 cut(s) 123, 286
DdeI CTNAG 4 cut(s) 32, 50, 143, 321
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
DraIII CACNNNGTG 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 477
FaeI CATG 3 cut(s) 25, 152, 463
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FatI CATG 3 cut(s) 21, 148, 459
FauNDI CATATG 1 cut(s) 569
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 1 cut(s) 47
FokI GGATG 2 cut(s) 125, 484
Fsp4HI GCNGC 1 cut(s) 47
FspBI CTAG 2 cut(s) 537, 564
GlaI GCGC 1 cut(s) 503
GluI GCNGC 1 cut(s) 47
HapII CCGG 2 cut(s) 12, 102
HhaI GCGC 1 cut(s) 504
Hin1II CATG 3 cut(s) 25, 152, 463
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 515
HpaII CCGG 2 cut(s) 12, 102
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 2 cut(s) 295, 357
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 2 cut(s) 12, 102
Hpy8I GTNNAC 2 cut(s) 295, 357
HpyCH4IV ACGT 1 cut(s) 125
HpyF3I CTNAG 4 cut(s) 32, 50, 143, 321
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 3 cut(s) 25, 152, 463
HspAI GCGC 1 cut(s) 502
Kpn2I TCCGGA 2 cut(s) 11, 101
Kzo9I GATC 1 cut(s) 95
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 5 cut(s) 70, 144, 438, 462, 530
MaeI CTAG 2 cut(s) 537, 564
MaeII ACGT 1 cut(s) 125
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 2 cut(s) 90, 455
MflI RGATCY 1 cut(s) 95
MluCI AATT 4 cut(s) 153, 186, 385, 447
MnlI CCTC 1 cut(s) 152
Mph1103I ATGCAT 1 cut(s) 477
MroI TCCGGA 2 cut(s) 11, 101
MseI TTAA 2 cut(s) 185, 527
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 2 cut(s) 12, 102
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 569
NdeII GATC 1 cut(s) 95
NlaIII CATG 3 cut(s) 25, 152, 463
NsiI ATGCAT 1 cut(s) 477
NspI RCATGY 2 cut(s) 152, 463
PciI ACATGT 1 cut(s) 459
PkrI GCNGC 1 cut(s) 48
PscI ACATGT 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspPI GGNCC 1 cut(s) 104
PstI CTGCAG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 2 cut(s) 124, 287
RsaNI GTAC 2 cut(s) 123, 286
SaqAI TTAA 2 cut(s) 185, 527
SatI GCNGC 1 cut(s) 47
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 120
SetI ASST 6 cut(s) 51, 109, 124, 128, 287, 519
SfaNI GCATC 5 cut(s) 70, 144, 438, 462, 530
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 536
Sse9I AATT 4 cut(s) 153, 186, 385, 447
SspMI CTAG 2 cut(s) 537, 564
StyD4I CCNGG 1 cut(s) 118
TaiI ACGT 1 cut(s) 128
TasI AATT 4 cut(s) 153, 186, 385, 447
Tru1I TTAA 2 cut(s) 185, 527
Tru9I TTAA 2 cut(s) 185, 527
TscAI CASTG 2 cut(s) 322, 439
TseI GCWGC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 171, 471
TspRI CASTG 2 cut(s) 322, 439
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 1 cut(s) 447
XceI RCATGY 2 cut(s) 152, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 356
XspI CTAG 2 cut(s) 537, 564
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.