Rorug07G0172500

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008140
Physical Location & Seq
Forward (+)
14130353 .. 14132039
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0172500.1

Sequence Viewer

Length: 588 bp
ATGGCGGAGATGGATCTAACACCAAAGTTAGCGGCAGTAGAGTTCGAGGGAGATGGTGGAGGATATCACACATGGTCAGCTCCAGCACTTGGCAAGGCCAAAGTTGGTGCCGGCAAGCTTGTTTTAAAGCCGAGTGGTTTTGCTCTTCCTCACTATGCAGATTCTGCCAAACTGGGGTATGTTGTTCAAGGTGAGGATGGAGTAATTGGGATGGTTTTACCCAACACATCAGAGGAGGTGGTGTTGAAGCTTAAGAAAGGAGATGTGATTCCGGTACCAGTCGGAGCAGTCTCATGGTGGTTCAACAATGGCGACTCACCCGATGACCTTGTTATCATTTTCTTGGGCGATCTACAAAGGCCTACACTCCTGATTTGGGACACAGCAGGACAAGAAAGGTTTCAAGGTCTCAGGGGCAGCATTTTATGTGGAGCAGATTGTTGTGTTATTGTTTATAATGTGAATGTTCTAAGATCATTTGAAACACTTCAGAACTGGCATGAAGAGTTTCTCATACAGGTAGGTCCAGCTGACACTGAGGCATTTCCATTTATACTACTCGGCAACAAAGTTGATATAGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

195

Amino Acids

21.0

Weight (kDa)

4.57

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 15 - 114 1.9e-13 Cupin
Ras PF00071 120 - 194 1.2e-17 Ras family
Roc PF08477 121 - 192 2.6e-10 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 274
AccB1I GGYRCC 2 cut(s) 107, 274
AccB7I CCANNNNNTGG 1 cut(s) 89
AciI CCGC 2 cut(s) 5, 32
AclWI GGATC 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 89, 174, 376
AflII CTTAAG 1 cut(s) 251
AgsI TTSAA 5 cut(s) 188, 247, 304, 404, 482
AluBI AGCT 4 cut(s) 80, 118, 250, 530
AluI AGCT 4 cut(s) 80, 118, 250, 530
Alw26I GTCTC 2 cut(s) 295, 413
AlwI GGATC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 2 cut(s) 96, 359
ApeKI GCWGC 1 cut(s) 417
Asp700I GAANNNNTTC 3 cut(s) 399, 486, 507
Asp718I GGTACC 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 203, 309
AvaII GGWCC 1 cut(s) 524
BanI GGYRCC 2 cut(s) 107, 274
BbvI GCAGC 1 cut(s) 429
BccI CCATC 5 cut(s) 4, 47, 191, 205, 575
BcoDI GTCTC 2 cut(s) 295, 413
BfrI CTTAAG 1 cut(s) 251
BisI GCNGC 2 cut(s) 33, 418
BlsI GCNGC 2 cut(s) 34, 419
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 2 cut(s) 109, 276
BmrI ACTGGG 1 cut(s) 182
BmuI ACTGGG 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 413
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 174, 376
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 177, 278, 500
BseGI GGATG 2 cut(s) 202, 216
BseLI CCNNNNNNNGG 3 cut(s) 89, 174, 376
BseMII CTCAG 2 cut(s) 424, 528
BseNI ACTGG 3 cut(s) 177, 278, 500
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 429
BshFI GGCC 2 cut(s) 98, 361
BshNI GGYRCC 2 cut(s) 107, 274
BsiSI CCGG 2 cut(s) 111, 272
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 3 cut(s) 89, 174, 376
BsmAI GTCTC 2 cut(s) 295, 413
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 2 cut(s) 98, 361
Bso31I GGTCTC 1 cut(s) 413
Bsp143I GATC 3 cut(s) 13, 349, 473
BspACI CCGC 2 cut(s) 5, 32
BspANI GGCC 2 cut(s) 98, 361
BspCNI CTCAG 2 cut(s) 423, 529
BspLI GGNNCC 2 cut(s) 109, 276
BspPI GGATC 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 150
BspT107I GGYRCC 2 cut(s) 107, 274
BspTI CTTAAG 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 3 cut(s) 177, 278, 500
BssAI RCCGGY 1 cut(s) 110
BssMI GATC 3 cut(s) 13, 349, 473
Bst6I CTCTTC 2 cut(s) 150, 498
BstAFI CTTAAG 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 112, 116
BstDEI CTNAG 3 cut(s) 410, 470, 537
BstF5I GGATG 2 cut(s) 202, 216
BstKTI GATC 3 cut(s) 16, 352, 476
BstMAI GTCTC 2 cut(s) 295, 413
BstMBI GATC 3 cut(s) 13, 349, 473
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 2 cut(s) 98, 361
BtsCI GGATG 2 cut(s) 202, 216
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 2 cut(s) 112, 116
CaiI CAGNNNCTG 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 275
CviAII CATG 3 cut(s) 72, 294, 500
CviJI RGCY 8 cut(s) 80, 98, 118, 130, 250, 361, 530, 585
CviKI_1 RGCY 8 cut(s) 80, 98, 118, 130, 250, 361, 530, 585
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 3 cut(s) 410, 470, 537
DpnI GATC 3 cut(s) 15, 351, 475
DpnII GATC 3 cut(s) 13, 349, 473
DraI TTTAAA 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 150, 498
EarI CTCTTC 2 cut(s) 150, 498
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 524
Eco57I CTGAAG 1 cut(s) 473
EcoRV GATATC 1 cut(s) 65
FaeI CATG 3 cut(s) 75, 297, 503
FaqI GGGAC 1 cut(s) 392
FatI CATG 3 cut(s) 71, 293, 499
Fnu4HI GCNGC 2 cut(s) 33, 418
FokI GGATG 2 cut(s) 209, 223
Fsp4HI GCNGC 2 cut(s) 33, 418
GluI GCNGC 2 cut(s) 33, 418
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 2 cut(s) 98, 361
HapII CCGG 2 cut(s) 111, 272
Hin1II CATG 3 cut(s) 75, 297, 503
HindIII AAGCTT 2 cut(s) 116, 248
HinfI GANTC 3 cut(s) 161, 268, 314
HpaII CCGG 2 cut(s) 111, 272
HphI GGTGA 2 cut(s) 203, 309
Hpy188I TCNGA 3 cut(s) 232, 284, 492
Hpy188III TCNNGA 1 cut(s) 370
HpyCH4V TGCA 1 cut(s) 158
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 410, 470, 537
Hsp92II CATG 3 cut(s) 75, 297, 503
KpnI GGTACC 1 cut(s) 278
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 3 cut(s) 13, 349, 473
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 3 cut(s) 85, 284, 431
Lsp1109I GCAGC 1 cut(s) 429
MalI GATC 3 cut(s) 15, 351, 475
MboI GATC 3 cut(s) 13, 349, 473
MboII GAAGA 2 cut(s) 137, 515
MflI RGATCY 1 cut(s) 13
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 7 cut(s) 40, 53, 159, 187, 226, 229, 532
MroNI GCCGGC 1 cut(s) 110
MroXI GAANNNNTTC 3 cut(s) 399, 486, 507
MseI TTAA 2 cut(s) 125, 252
MspA1I CMGCKG 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 2 cut(s) 111, 272
MwoI GCNNNNNNNGC 1 cut(s) 164
NaeI GCCGGC 1 cut(s) 112
NdeII GATC 3 cut(s) 13, 349, 473
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 3 cut(s) 75, 297, 503
NlaIV GGNNCC 2 cut(s) 109, 276
NmeAIII GCCGAG 2 cut(s) 156, 540
PceI AGGCCT 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 112
PdmI GAANNNNTTC 3 cut(s) 399, 486, 507
PfeI GAWTC 2 cut(s) 161, 268
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 2 cut(s) 34, 419
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PsiI TTATAA 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 109, 276
PspPI GGNCC 1 cut(s) 524
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 125, 252
SatI GCNGC 2 cut(s) 33, 418
Sau3AI GATC 3 cut(s) 13, 349, 473
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 1 cut(s) 308
SinI GGWCC 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 1 cut(s) 204
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 2 cut(s) 5, 32
StuI AGGCCT 1 cut(s) 361
TaqI TCGA 1 cut(s) 45
TasI AATT 1 cut(s) 204
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 161, 268
Tru1I TTAA 2 cut(s) 125, 252
Tru9I TTAA 2 cut(s) 125, 252
TscAI CASTG 1 cut(s) 541
TseI GCWGC 1 cut(s) 417
TspDTI ATGAA 1 cut(s) 516
TspRI CASTG 1 cut(s) 541
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 524
XmnI GAANNNNTTC 3 cut(s) 399, 486, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.