Rh7DG313100

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008140
Physical Location & Seq
Reverse (-)
36721105 .. 36722406
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG313100.1

Sequence Viewer

Length: 1302 bp
ATGGCTACTGAAGCAATGAAAGTAATAAAGCAAGCCATGAACACTGAGAAGAGATGTTCAAGCTCAAGCTCCATAGTAGGAGGACACTTCAACGATCCACATCAAGCTCATTTACTGTGCGCAAACCAATTCTGCCAGTGTGAATCTCTGTCATTGAGTAGCAAGCTCACATATAATCCTGCTCCATCGAGTTCAATCGCTCCAACCCAGCAGCATTCGGAGCATCTGCAGCAGCCATTTCTTGCTTTATCGTCCACACATCAGCAGACTTCAAGAATGAAAGTAAAATCCCAACACAAAGAGGGGATTGTATCTTGGCAGTCATCCAAAATGCGCAGAGCCACTAGACGAATAAAGGTAATCAGTTTATTTAAAAGGTTGAATGATGGAACAAAGCGAAACAACTTTCTGAGGGGAAATTGTCAAGACAACTCAAGGGAAGAAAATGATCACACCAGCCAGGACAAAGTATTTCACTGCGGCATAAATTCTGTTGAATTGAATAGCAGAGCCCGATGGAGCCTCTATACAGATAACTGTTACTTGAACCAAAATCTCAGCAACAAGTACCGACCACGGCATCTCGAGGACATTGCAGGCAATGAAATGGTTATTAAGGCCATAACCAATGCAATTCAGCAGAATAAGATTGCACCGCTTTATCTTTTCTATGGTGCAAGAGGCTCCGGGAAGACATCTATTGCTAGAATATTAGCATTGGCCTTGAACTGTGAATCCAGCTCATTTATCAAACCGTGCTGGAAATGCCAAGGATGCTCCAGAAGCCTTTCTATTATGGAGTTGTGTTCTGGAAGTACAGTTGCTGGATTCAAAAGGATTAGGAAACTCCTTCAAGGTATATCATCTTTGCAAGCAACACCAGGATTTAAGGTATTTATCCTTGAAGAGTGCGAATCGTTTAGTACAGAAGCATGGGACGAACTTTCAGGCATAGCAAATGGAGCTTACAATGCTGGTGTGGTATTCATCCTTATCACTACAAATGCGGACATGGTGCCTAGAACCATATCATCAAGATGTCAGAAATTCTTCTTTCCCAAACTCAGAGACACTGATATCATGCTCAAGTTGACTAGAATTGCAGCCCAAGAGGGTATAAGGATTGAAAAAGAAGCAATCAAACTGGTTACTACCAAAGCACAAGGATCTTTGCGAGAAGCTGAAAACATATTAGACCAGCTGATATTACTGGGGCCAAAGATAACAAGTTCCATGGCACAACAGCTTGTAGGTGGTCCTTTCTTTAAAACTTTCTACTCTCTCGGGGTTCAGTATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

433

Amino Acids

48.37

Weight (kDa)

9.46

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 201 - 355 9.1e-20 DNA polymerase III, delta subunit
AAA PF00004 221 - 352 9.2e-09 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 360 - 405 3.6e-08 DNA polymerase III clamp loader subunit, ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 121, 335
AccB1I GGYRCC 1 cut(s) 1015
AciI CCGC 3 cut(s) 480, 656, 1007
AclWI GGATC 2 cut(s) 89, 1174
AcsI RAATTY 2 cut(s) 487, 1046
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 3 cut(s) 569, 817, 925
AjnI CCWGG 2 cut(s) 459, 880
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
AluBI AGCT 9 cut(s) 63, 69, 107, 166, 741, 965, 1181, 1201, 1246
AluI AGCT 9 cut(s) 63, 69, 107, 166, 741, 965, 1181, 1201, 1246
Alw26I GTCTC 1 cut(s) 1062
AlwI GGATC 2 cut(s) 89, 1174
AlwNI CAGNNNCTG 1 cut(s) 824
Ama87I CYCGRG 2 cut(s) 584, 1283
AoxI GGCC 3 cut(s) 618, 720, 1214
ApeKI GCWGC 4 cut(s) 211, 229, 232, 1103
ApoI RAATTY 2 cut(s) 487, 1046
Asp700I GAANNNNTTC 2 cut(s) 787, 1049
AspLEI GCGC 2 cut(s) 122, 336
AspS9I GGNCC 2 cut(s) 1214, 1256
AsuC2I CCSGG 1 cut(s) 688
AvaI CYCGRG 2 cut(s) 584, 1283
AvaII GGWCC 1 cut(s) 1256
BanI GGYRCC 1 cut(s) 1015
BanII GRGCYC 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 698
BbvI GCAGC 4 cut(s) 223, 241, 244, 1115
BccI CCATC 3 cut(s) 193, 380, 510
BceAI ACGGC 1 cut(s) 593
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 2 cut(s) 461, 882
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 688
BcoDI GTCTC 1 cut(s) 1062
BfaI CTAG 4 cut(s) 345, 705, 1020, 1095
BfmI CTRYAG 1 cut(s) 227
BisI GCNGC 5 cut(s) 212, 230, 233, 481, 1104
BlsI GCNGC 5 cut(s) 213, 231, 234, 482, 1105
Bme1390I CCNGG 3 cut(s) 461, 688, 882
Bme18I GGWCC 1 cut(s) 1256
BmeT110I CYCGRG 2 cut(s) 584, 1283
BmgT120I GGNCC 2 cut(s) 1214, 1256
BmiI GGNNCC 4 cut(s) 521, 685, 1017, 1215
BmrFI CCNGG 3 cut(s) 461, 688, 882
BmrI ACTGGG 1 cut(s) 1220
BmsI GCATC 3 cut(s) 232, 589, 764
BmuI ACTGGG 1 cut(s) 1220
BpiI GAAGAC 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 763
BpuEI CTTGAG 3 cut(s) 49, 418, 1070
BpuMI CCSGG 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 575, 769, 1233
Bse1I ACTGG 3 cut(s) 136, 1149, 1215
Bse3DI GCAATG 3 cut(s) 21, 591, 607
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 2 cut(s) 461, 882
BseDI CCNNGG 3 cut(s) 575, 769, 1233
BseGI GGATG 3 cut(s) 323, 779, 987
BseJI GATNNNNATC 1 cut(s) 99
BseMI GCAATG 3 cut(s) 21, 591, 607
BseMII CTCAG 4 cut(s) 36, 401, 571, 1078
BseNI ACTGG 3 cut(s) 136, 1149, 1215
BseXI GCAGC 4 cut(s) 223, 241, 244, 1115
BseYI CCCAGC 1 cut(s) 207
BshFI GGCC 3 cut(s) 620, 722, 1216
BshNI GGYRCC 1 cut(s) 1015
BsiHKCI CYCGRG 2 cut(s) 584, 1283
BsiSI CCGG 1 cut(s) 687
BslFI GGGAC 1 cut(s) 950
BsmAI GTCTC 1 cut(s) 1062
BsmFI GGGAC 1 cut(s) 950
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 3 cut(s) 620, 722, 1216
BsoBI CYCGRG 2 cut(s) 584, 1283
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 3 cut(s) 94, 448, 1166
Bsp19I CCATGG 1 cut(s) 1233
BspACI CCGC 3 cut(s) 480, 656, 1007
BspANI GGCC 3 cut(s) 620, 722, 1216
BspCNI CTCAG 4 cut(s) 37, 402, 570, 1077
BspLI GGNNCC 4 cut(s) 521, 685, 1017, 1215
BspMAI CTGCAG 1 cut(s) 231
BspPI GGATC 2 cut(s) 89, 1174
BspT107I GGYRCC 1 cut(s) 1015
BsrDI GCAATG 3 cut(s) 21, 591, 607
BsrI ACTGG 3 cut(s) 136, 1149, 1215
BssECI CCNNGG 3 cut(s) 575, 769, 1233
BssMI GATC 3 cut(s) 94, 448, 1166
BssT1I CCWWGG 2 cut(s) 769, 1233
Bst2UI CCWGG 2 cut(s) 461, 882
Bst4CI ACNGT 5 cut(s) 117, 539, 731, 756, 820
Bst6I CTCTTC 2 cut(s) 44, 900
BstC8I GCNNGC 4 cut(s) 33, 164, 598, 873
BstDEI CTNAG 4 cut(s) 45, 410, 557, 1064
BstDSI CCRYGG 2 cut(s) 575, 1233
BstF5I GGATG 3 cut(s) 323, 779, 987
BstHHI GCGC 2 cut(s) 122, 336
BstKTI GATC 3 cut(s) 97, 451, 1169
BstMAI GTCTC 1 cut(s) 1062
BstMBI GATC 3 cut(s) 94, 448, 1166
BstMWI GCNNNNNNNGC 8 cut(s) 11, 220, 229, 765, 774, 783, 962, 971
BstNI CCWGG 2 cut(s) 461, 882
BstSCI CCNGG 3 cut(s) 459, 686, 880
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 4 cut(s) 223, 241, 244, 1115
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 1 cut(s) 1166
BstYI RGATCY 1 cut(s) 1166
BsuRI GGCC 3 cut(s) 620, 722, 1216
BtgI CCRYGG 2 cut(s) 575, 1233
BtsCI GGATG 3 cut(s) 323, 779, 987
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 4 cut(s) 42, 143, 475, 1071
Cac8I GCNNGC 4 cut(s) 33, 164, 598, 873
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 2 cut(s) 122, 336
Cfr13I GGNCC 2 cut(s) 1214, 1256
Csp6I GTAC 3 cut(s) 568, 816, 924
CviAII CATG 5 cut(s) 37, 933, 1012, 1081, 1234
CviQI GTAC 3 cut(s) 568, 816, 924
DdeI CTNAG 4 cut(s) 45, 410, 557, 1064
DpnI GATC 3 cut(s) 96, 450, 1168
DpnII GATC 3 cut(s) 94, 448, 1166
DraI TTTAAA 2 cut(s) 373, 1267
Eam1104I CTCTTC 2 cut(s) 44, 900
EarI CTCTTC 2 cut(s) 44, 900
Eco130I CCWWGG 2 cut(s) 769, 1233
Eco24I GRGCYC 1 cut(s) 514
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 1 cut(s) 1256
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 584, 1283
EcoRII CCWGG 2 cut(s) 459, 880
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 2 cut(s) 769, 1233
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 2 cut(s) 769, 1233
FaeI CATG 5 cut(s) 40, 936, 1015, 1084, 1237
FaqI GGGAC 1 cut(s) 950
FatI CATG 5 cut(s) 36, 932, 1011, 1080, 1233
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 212, 230, 233, 481, 1104
FokI GGATG 3 cut(s) 310, 786, 974
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 5 cut(s) 212, 230, 233, 481, 1104
FspBI CTAG 4 cut(s) 345, 705, 1020, 1095
FspI TGCGCA 2 cut(s) 121, 335
GlaI GCGC 2 cut(s) 121, 335
GluI GCNGC 5 cut(s) 212, 230, 233, 481, 1104
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 763
HaeIII GGCC 3 cut(s) 620, 722, 1216
HapII CCGG 1 cut(s) 687
HhaI GCGC 2 cut(s) 122, 336
Hin1II CATG 5 cut(s) 40, 936, 1015, 1084, 1237
Hin6I GCGC 2 cut(s) 120, 334
HinP1I GCGC 2 cut(s) 120, 334
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HinfI GANTC 4 cut(s) 143, 734, 828, 914
HpaII CCGG 1 cut(s) 687
Hpy166II GTNNAC 2 cut(s) 255, 1092
Hpy188I TCNGA 4 cut(s) 220, 411, 1044, 1067
Hpy188III TCNNGA 6 cut(s) 273, 425, 584, 780, 810, 1035
Hpy8I GTNNAC 2 cut(s) 255, 1092
HpyAV CCTTC 1 cut(s) 860
HpyCH4III ACNGT 5 cut(s) 117, 539, 731, 756, 820
HpyCH4V TGCA 7 cut(s) 229, 596, 632, 653, 677, 871, 1103
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 220, 229, 765, 774, 783, 962, 971
HpyF3I CTNAG 4 cut(s) 45, 410, 557, 1064
Hsp92II CATG 5 cut(s) 40, 936, 1015, 1084, 1237
HspAI GCGC 2 cut(s) 120, 334
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 3 cut(s) 94, 448, 1166
LmnI GCTCC 8 cut(s) 74, 187, 205, 220, 519, 689, 782, 962
Lsp1109I GCAGC 4 cut(s) 223, 241, 244, 1115
LweI GCATC 3 cut(s) 232, 589, 764
MaeI CTAG 4 cut(s) 345, 705, 1020, 1095
MaeIII GTNAC 2 cut(s) 539, 1147
MalI GATC 3 cut(s) 96, 450, 1168
MboI GATC 3 cut(s) 94, 448, 1166
MboII GAAGA 5 cut(s) 61, 452, 703, 917, 1042
MflI RGATCY 1 cut(s) 1166
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 7 cut(s) 128, 418, 487, 497, 633, 1046, 1098
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 7 cut(s) 74, 295, 405, 533, 580, 674, 1105
MroXI GAANNNNTTC 2 cut(s) 787, 1049
MseI TTAA 4 cut(s) 372, 615, 888, 1266
MslI CAYNNNNRTG 1 cut(s) 1036
MspA1I CMGCKG 1 cut(s) 1201
MspI CCGG 1 cut(s) 687
MspR9I CCNGG 3 cut(s) 461, 688, 882
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 2 cut(s) 461, 882
MwoI GCNNNNNNNGC 8 cut(s) 11, 220, 229, 765, 774, 783, 962, 971
NciI CCSGG 1 cut(s) 688
NcoI CCATGG 1 cut(s) 1233
NdeII GATC 3 cut(s) 94, 448, 1166
NlaIII CATG 5 cut(s) 40, 936, 1015, 1084, 1237
NlaIV GGNNCC 4 cut(s) 521, 685, 1017, 1215
NsbI TGCGCA 2 cut(s) 121, 335
PaeR7I CTCGAG 1 cut(s) 584
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 787, 1049
PfeI GAWTC 4 cut(s) 143, 734, 828, 914
PfoI TCCNGGA 1 cut(s) 686
PkrI GCNGC 5 cut(s) 213, 231, 234, 482, 1105
Psp6I CCWGG 2 cut(s) 459, 880
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 2 cut(s) 459, 880
PspN4I GGNNCC 4 cut(s) 521, 685, 1017, 1215
PspPI GGNCC 2 cut(s) 1214, 1256
PstI CTGCAG 1 cut(s) 231
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 1 cut(s) 1166
PvuII CAGCTG 1 cut(s) 1201
RsaI GTAC 3 cut(s) 569, 817, 925
RsaNI GTAC 3 cut(s) 568, 816, 924
RseI CAYNNNNRTG 1 cut(s) 1036
SaqAI TTAA 4 cut(s) 372, 615, 888, 1266
SatI GCNGC 5 cut(s) 212, 230, 233, 481, 1104
Sau3AI GATC 3 cut(s) 94, 448, 1166
Sau96I GGNCC 2 cut(s) 1214, 1256
ScrFI CCNGG 3 cut(s) 461, 688, 882
SduI GDGCHC 1 cut(s) 514
SfaNI GCATC 3 cut(s) 232, 589, 764
SfcI CTRYAG 1 cut(s) 227
Sfr274I CTCGAG 1 cut(s) 584
SinI GGWCC 1 cut(s) 1256
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 1036
SmlI CTYRAG 4 cut(s) 64, 433, 584, 1085
SmoI CTYRAG 4 cut(s) 64, 433, 584, 1085
Sse9I AATT 7 cut(s) 128, 418, 487, 497, 633, 1046, 1098
SsiI CCGC 3 cut(s) 480, 656, 1007
SspI AATATT 1 cut(s) 711
SspMI CTAG 4 cut(s) 345, 705, 1020, 1095
StyD4I CCNGG 3 cut(s) 459, 686, 880
StyI CCWWGG 2 cut(s) 769, 1233
TaaI ACNGT 5 cut(s) 117, 539, 731, 756, 820
TaqI TCGA 2 cut(s) 188, 585
TasI AATT 7 cut(s) 128, 418, 487, 497, 633, 1046, 1098
TatI WGTACW 2 cut(s) 815, 923
TauI GCSGC 1 cut(s) 483
TfiI GAWTC 4 cut(s) 143, 734, 828, 914
Tru1I TTAA 4 cut(s) 372, 615, 888, 1266
Tru9I TTAA 4 cut(s) 372, 615, 888, 1266
TscAI CASTG 4 cut(s) 49, 143, 482, 1078
TseI GCWGC 4 cut(s) 211, 229, 232, 1103
TspDTI ATGAA 5 cut(s) 32, 53, 293, 618, 976
TspRI CASTG 4 cut(s) 49, 143, 482, 1078
VpaK11BI GGWCC 1 cut(s) 1256
XapI RAATTY 2 cut(s) 487, 1046
XhoI CTCGAG 1 cut(s) 584
XmnI GAANNNNTTC 2 cut(s) 787, 1049
XspI CTAG 4 cut(s) 345, 705, 1020, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.