Rh7AG314900

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008140
Physical Location & Seq
Reverse (-)
35350396 .. 35351715
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG314900.1

Sequence Viewer

Length: 1320 bp
ATGGCTACTGAAGCAATTAAAGTATTGAGAAAGCAAGCCAAGAACACTGAGAAGAGATGTTCAAGCTCAAGCTCCATAGTAGGAGGACACTCCAACGATCCACATCAAGCTCATCTACTGTGTGCAAACCAATTCTGCCAGTGTGAATCTCTGTCATTGAGTAGCAAGCTCACATATAATCCTGCTCCATCGAGTTCAATAGCTCCAACCCAGCAGCATTCGGAGCATCTGCAGCAGTCATTTCTTGCTTCATCGTCCACAAATCAGCAGACTTCAAGAATGAAAGTGAAATCCCAACACAAAGAGGGGATTGTATCTTGGCAGTCATCCAAAATGCGCAGAGCCACTAGACGAATAAAGGTAATCAGTTTATTTAAAAGATTGAATGATGGAACAAAGCGAAGCAACTTTCTGAGGGGTAATTGTCAAGAAAAATCAAGGGAAGAAAATGATCATACCAGCCAGGACAAAGTATTTCACTGCGGCATAAATTCTGTTGAATTGAATAGCAGAGCCCAATGGAGCCTCTATACAGATAACTGCTACTTGAACCAAAATCTCAGCAACAAGTACCGACCACGGCATCTCAAGGACATTGCAGGCCATGAAATGGTTATTAAGGCCATAACCAATGCAATTCAGCAGAATAAGATTGCACCGCTTTATCTTTTCTATGGTGCAAGAGGCTCCGGGAAGACATCTATTGCTAGAATATTAGCATTGGCCTTGAACTGTGAATCCAGCTCATTTATCAAACCGTGCTGGAAATGCCAAGGATGCTCCAGAAGCCTTTCTATTATGGAGTTGTGTTCTGGAGGTACAGTTGCTGGATTCAAAAGGATTAGGAAACTCCTTCAAAGTATATCATCTTTGCAAGCAACACCAGGATTTAAGGTATTTATCCTTGAAGAGTGCGAATCGTTTAGTACAGAAGCATGGGACGAACTTTCAGGCATAGCAAATGGAGCTTACAATGCTGGTGTGGTATTCATCCTTATCACTACAAATGCAGACATGGTTCCTAGAACCATATCATCAAGATGTCAGAAATTCTTCTTTCCCAAACTCAGAGACACTGATATCATGCTCAAGTTGACTAGAATTGCAGCCCAGGAGGGTATAAGGATTGAAAAAGAAGCAATCAAACTGGTTACTACCAAAGCACAAGGATCTTTGCGAGAAGCTGAAAACATATTAGACCAGCTGATATTACTGGGGCCAAAGATAACAAGTTCCATGGCACAACAGCTTGTAGGTGGTCCTTTCTTTAAAACTTTCTACTCTCTCTTGGAGTTAAGTATACCTAATGGGGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

439

Amino Acids

48.86

Weight (kDa)

9.57

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 202 - 356 2.7e-20 DNA polymerase III, delta subunit
AAA PF00004 222 - 353 5.1e-09 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 361 - 406 3.7e-08 DNA polymerase III clamp loader subunit, ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 610
AccI GTMKAC 1 cut(s) 1300
AciI CCGC 2 cut(s) 483, 659
AclWI GGATC 2 cut(s) 92, 1177
AcsI RAATTY 2 cut(s) 490, 1049
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 3 cut(s) 572, 820, 928
AfiI CCNNNNNNNGG 1 cut(s) 610
AjnI CCWGG 3 cut(s) 462, 883, 1110
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
Alw26I GTCTC 1 cut(s) 1065
AlwI GGATC 2 cut(s) 92, 1177
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 4 cut(s) 601, 621, 723, 1217
ApeKI GCWGC 3 cut(s) 214, 232, 1106
ApoI RAATTY 2 cut(s) 490, 1049
Asp700I GAANNNNTTC 2 cut(s) 790, 1052
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 2 cut(s) 1217, 1259
AsuC2I CCSGG 1 cut(s) 691
AvaII GGWCC 1 cut(s) 1259
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 701
BbvI GCAGC 3 cut(s) 226, 244, 1118
BccI CCATC 2 cut(s) 196, 383
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 3 cut(s) 464, 885, 1112
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 1 cut(s) 1065
BfaI CTAG 4 cut(s) 348, 708, 1023, 1098
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 4 cut(s) 215, 233, 484, 1107
BlsI GCNGC 4 cut(s) 216, 234, 485, 1108
Bme1390I CCNGG 4 cut(s) 464, 691, 885, 1112
Bme18I GGWCC 1 cut(s) 1259
BmgT120I GGNCC 2 cut(s) 1217, 1259
BmiI GGNNCC 4 cut(s) 524, 688, 1020, 1218
BmrFI CCNGG 4 cut(s) 464, 691, 885, 1112
BmrI ACTGGG 1 cut(s) 1223
BmsI GCATC 3 cut(s) 235, 592, 767
BmuI ACTGGG 1 cut(s) 1223
BpiI GAAGAC 1 cut(s) 701
BpmI CTGGAG 2 cut(s) 766, 834
BpuEI CTTGAG 3 cut(s) 52, 572, 1073
BpuMI CCSGG 1 cut(s) 691
BsaBI GATNNNNATC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 578, 772, 1110, 1236
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse1I ACTGG 3 cut(s) 139, 1152, 1218
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 3 cut(s) 464, 885, 1112
BseDI CCNNGG 4 cut(s) 578, 772, 1110, 1236
BseGI GGATG 3 cut(s) 326, 782, 990
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 4 cut(s) 39, 404, 574, 1081
BseNI ACTGG 3 cut(s) 139, 1152, 1218
BseXI GCAGC 3 cut(s) 226, 244, 1118
BseYI CCCAGC 1 cut(s) 210
BshFI GGCC 4 cut(s) 603, 623, 725, 1219
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 953
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 1 cut(s) 1065
BsmFI GGGAC 1 cut(s) 953
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 4 cut(s) 603, 623, 725, 1219
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 3 cut(s) 97, 451, 1169
Bsp19I CCATGG 1 cut(s) 1236
BspACI CCGC 2 cut(s) 483, 659
BspANI GGCC 4 cut(s) 603, 623, 725, 1219
BspCNI CTCAG 4 cut(s) 40, 405, 573, 1080
BspLI GGNNCC 4 cut(s) 524, 688, 1020, 1218
BspMAI CTGCAG 1 cut(s) 234
BspPI GGATC 2 cut(s) 92, 1177
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 3 cut(s) 139, 1152, 1218
BssECI CCNNGG 4 cut(s) 578, 772, 1110, 1236
BssMI GATC 3 cut(s) 97, 451, 1169
BssNAI GTATAC 1 cut(s) 1301
BssT1I CCWWGG 2 cut(s) 772, 1236
Bst1107I GTATAC 1 cut(s) 1301
Bst2UI CCWGG 3 cut(s) 464, 885, 1112
Bst4CI ACNGT 4 cut(s) 120, 734, 759, 823
Bst6I CTCTTC 2 cut(s) 47, 903
BstC8I GCNNGC 4 cut(s) 36, 167, 601, 876
BstDEI CTNAG 4 cut(s) 48, 413, 560, 1067
BstDSI CCRYGG 2 cut(s) 578, 1236
BstF5I GGATG 3 cut(s) 326, 782, 990
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 3 cut(s) 100, 454, 1172
BstMAI GTCTC 1 cut(s) 1065
BstMBI GATC 3 cut(s) 97, 451, 1169
BstMWI GCNNNNNNNGC 8 cut(s) 11, 223, 232, 768, 777, 786, 965, 974
BstNI CCWGG 3 cut(s) 464, 885, 1112
BstSCI CCNGG 4 cut(s) 462, 689, 883, 1110
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 3 cut(s) 226, 244, 1118
BstV2I GAAGAC 1 cut(s) 701
BstX2I RGATCY 1 cut(s) 1169
BstYI RGATCY 1 cut(s) 1169
BstZ17I GTATAC 1 cut(s) 1301
BsuRI GGCC 4 cut(s) 603, 623, 725, 1219
BtgI CCRYGG 2 cut(s) 578, 1236
BtsCI GGATG 3 cut(s) 326, 782, 990
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 4 cut(s) 45, 146, 478, 1074
Cac8I GCNNGC 4 cut(s) 36, 167, 601, 876
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 2 cut(s) 1217, 1259
Csp6I GTAC 3 cut(s) 571, 819, 927
CviAII CATG 5 cut(s) 605, 936, 1015, 1084, 1237
CviQI GTAC 3 cut(s) 571, 819, 927
DdeI CTNAG 4 cut(s) 48, 413, 560, 1067
DpnI GATC 3 cut(s) 99, 453, 1171
DpnII GATC 3 cut(s) 97, 451, 1169
DraI TTTAAA 2 cut(s) 376, 1270
Eam1104I CTCTTC 2 cut(s) 47, 903
EarI CTCTTC 2 cut(s) 47, 903
Eco130I CCWWGG 2 cut(s) 772, 1236
Eco24I GRGCYC 1 cut(s) 517
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 1 cut(s) 1259
Eco57I CTGAAG 1 cut(s) 30
EcoRII CCWGG 3 cut(s) 462, 883, 1110
EcoRV GATATC 1 cut(s) 1081
EcoT14I CCWWGG 2 cut(s) 772, 1236
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 2 cut(s) 772, 1236
FaeI CATG 5 cut(s) 608, 939, 1018, 1087, 1240
FaqI GGGAC 1 cut(s) 953
FatI CATG 5 cut(s) 604, 935, 1014, 1083, 1236
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 1300
Fnu4HI GCNGC 4 cut(s) 215, 233, 484, 1107
FokI GGATG 3 cut(s) 313, 789, 977
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 4 cut(s) 215, 233, 484, 1107
FspBI CTAG 4 cut(s) 348, 708, 1023, 1098
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 1 cut(s) 338
GluI GCNGC 4 cut(s) 215, 233, 484, 1107
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 2 cut(s) 766, 834
HaeIII GGCC 4 cut(s) 603, 623, 725, 1219
HapII CCGG 1 cut(s) 690
HhaI GCGC 1 cut(s) 339
Hin1II CATG 5 cut(s) 608, 939, 1018, 1087, 1240
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HincII GTYRAC 2 cut(s) 1095, 1315
HindII GTYRAC 2 cut(s) 1095, 1315
HinfI GANTC 4 cut(s) 146, 737, 831, 917
HpaII CCGG 1 cut(s) 690
Hpy166II GTNNAC 4 cut(s) 258, 1095, 1301, 1315
Hpy188I TCNGA 4 cut(s) 223, 414, 1047, 1070
Hpy188III TCNNGA 5 cut(s) 276, 428, 783, 813, 1038
Hpy8I GTNNAC 4 cut(s) 258, 1095, 1301, 1315
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 4 cut(s) 120, 734, 759, 823
HpyCH4V TGCA 9 cut(s) 125, 232, 599, 635, 656, 680, 874, 1010, 1106
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 223, 232, 768, 777, 786, 965, 974
HpyF3I CTNAG 4 cut(s) 48, 413, 560, 1067
Hsp92II CATG 5 cut(s) 608, 939, 1018, 1087, 1240
HspAI GCGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 3 cut(s) 97, 451, 1169
LmnI GCTCC 8 cut(s) 77, 190, 208, 223, 522, 692, 785, 965
Lsp1109I GCAGC 3 cut(s) 226, 244, 1118
LweI GCATC 3 cut(s) 235, 592, 767
MaeI CTAG 4 cut(s) 348, 708, 1023, 1098
MaeIII GTNAC 1 cut(s) 1150
MalI GATC 3 cut(s) 99, 453, 1171
MboI GATC 3 cut(s) 97, 451, 1169
MboII GAAGA 5 cut(s) 64, 455, 706, 920, 1045
MflI RGATCY 1 cut(s) 1169
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 8 cut(s) 15, 131, 421, 490, 500, 636, 1049, 1101
MmeI TCCRAC 2 cut(s) 117, 230
MnlI CCTC 7 cut(s) 77, 298, 408, 536, 677, 809, 1108
MroXI GAANNNNTTC 2 cut(s) 790, 1052
MseI TTAA 6 cut(s) 18, 375, 618, 891, 1269, 1295
MslI CAYNNNNRTG 1 cut(s) 1039
MspA1I CMGCKG 1 cut(s) 1204
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 4 cut(s) 464, 691, 885, 1112
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 3 cut(s) 464, 885, 1112
MwoI GCNNNNNNNGC 8 cut(s) 11, 223, 232, 768, 777, 786, 965, 974
NciI CCSGG 1 cut(s) 691
NcoI CCATGG 1 cut(s) 1236
NdeII GATC 3 cut(s) 97, 451, 1169
NlaIII CATG 5 cut(s) 608, 939, 1018, 1087, 1240
NlaIV GGNNCC 4 cut(s) 524, 688, 1020, 1218
NsbI TGCGCA 1 cut(s) 338
PctI GAATGC 1 cut(s) 217
PdmI GAANNNNTTC 2 cut(s) 790, 1052
PfeI GAWTC 4 cut(s) 146, 737, 831, 917
PflMI CCANNNNNTGG 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 689
PkrI GCNGC 4 cut(s) 216, 234, 485, 1108
Psp6I CCWGG 3 cut(s) 462, 883, 1110
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 3 cut(s) 462, 883, 1110
PspN4I GGNNCC 4 cut(s) 524, 688, 1020, 1218
PspPI GGNCC 2 cut(s) 1217, 1259
PstI CTGCAG 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 1 cut(s) 1169
PvuII CAGCTG 1 cut(s) 1204
RsaI GTAC 3 cut(s) 572, 820, 928
RsaNI GTAC 3 cut(s) 571, 819, 927
RseI CAYNNNNRTG 1 cut(s) 1039
SaqAI TTAA 6 cut(s) 18, 375, 618, 891, 1269, 1295
SatI GCNGC 4 cut(s) 215, 233, 484, 1107
Sau3AI GATC 3 cut(s) 97, 451, 1169
Sau96I GGNCC 2 cut(s) 1217, 1259
ScrFI CCNGG 4 cut(s) 464, 691, 885, 1112
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 3 cut(s) 235, 592, 767
SfcI CTRYAG 1 cut(s) 230
SinI GGWCC 1 cut(s) 1259
SmiMI CAYNNNNRTG 1 cut(s) 1039
SmlI CTYRAG 3 cut(s) 67, 587, 1088
SmoI CTYRAG 3 cut(s) 67, 587, 1088
Sse9I AATT 8 cut(s) 15, 131, 421, 490, 500, 636, 1049, 1101
SsiI CCGC 2 cut(s) 483, 659
SspI AATATT 1 cut(s) 714
SspMI CTAG 4 cut(s) 348, 708, 1023, 1098
StyD4I CCNGG 4 cut(s) 462, 689, 883, 1110
StyI CCWWGG 2 cut(s) 772, 1236
TaaI ACNGT 4 cut(s) 120, 734, 759, 823
TaqI TCGA 1 cut(s) 191
TasI AATT 8 cut(s) 15, 131, 421, 490, 500, 636, 1049, 1101
TatI WGTACW 1 cut(s) 926
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 4 cut(s) 146, 737, 831, 917
Tru1I TTAA 6 cut(s) 18, 375, 618, 891, 1269, 1295
Tru9I TTAA 6 cut(s) 18, 375, 618, 891, 1269, 1295
TscAI CASTG 4 cut(s) 52, 146, 485, 1081
TseI GCWGC 3 cut(s) 214, 232, 1106
TspDTI ATGAA 4 cut(s) 240, 296, 621, 979
TspRI CASTG 4 cut(s) 52, 146, 485, 1081
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 1 cut(s) 1259
XapI RAATTY 2 cut(s) 490, 1049
XmiI GTMKAC 1 cut(s) 1300
XmnI GAANNNNTTC 2 cut(s) 790, 1052
XspI CTAG 4 cut(s) 348, 708, 1023, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.