Rh7DG312900

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008140
Physical Location & Seq
Reverse (-)
36719213 .. 36719887
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG312900.1

Sequence Viewer

Length: 675 bp
ATGTCTGAAGAACTCCTATCGGAGACTCTAGAGAGCGCTATAGGATGCCCAATTATTCTAAATATGAGCCTAGAGCCTGTGGACTTGAAAATCTCAGACGAAAACTCGGCCTCCAGTTATCAGACACAGCTAGTGGAGAGCAGTCATTTTAACCATGGAACAAAATTTGTTCCTGAACCTGTTTATGAAGTAGATCTTGGAGCTGCAATGCAACACAATGGTCAGATGAAAACCTTCTCCTTAAACAGAGAGCAAGTTAAGGCCAACAAAGCTGATGGCTGCAAACTGATATCTAAAAGGCAAGACAAATACAGTACAAGCATAAGGGAGGAAACCATTGCTCAGCCACAGGAGATAACACCCTTCAATGATCTTTTAAGACAAAAGAACCAAGAGGATGCATCTATTGATTTGATAACAGATTGTTTGAGTTCTCTGATGGACACAACTCGTACAATGAGGACACAAAGGCCCCAGAAGAAGTGGTTGTCTGTATCCTCTGCTCAACGAAGTGACGCAAGCATTGAACCATACAGTCAAGATATATTGTTTGACAATGCAAAGACAAAGATAAAGAGAAGTGCAAAATCCCAAAAGCTTCCAAAAGCATTCTTTAAGGTTAAAGAACTTCATGATTCTCAGGATGGTCCAAGAAACAGGTACATACTAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

224

Amino Acids

25.39

Weight (kDa)

6.45

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 3 cut(s) 316, 454, 662
AfeI AGCGCT 1 cut(s) 37
AgsI TTSAA 3 cut(s) 88, 367, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 4 cut(s) 130, 203, 272, 598
AluI AGCT 4 cut(s) 130, 203, 272, 598
Alw26I GTCTC 1 cut(s) 17
Aor51HI AGCGCT 1 cut(s) 37
AoxI GGCC 3 cut(s) 108, 261, 470
ApeKI GCWGC 2 cut(s) 203, 279
ApoI RAATTY 1 cut(s) 164
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 471, 647
AvaII GGWCC 1 cut(s) 647
BbvI GCAGC 2 cut(s) 190, 266
BccI CCATC 3 cut(s) 269, 433, 638
BciVI GTATCC 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 3 cut(s) 29, 71, 131
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 1 cut(s) 505
BglII AGATCT 1 cut(s) 193
BisI GCNGC 2 cut(s) 204, 280
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 2 cut(s) 205, 281
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 2 cut(s) 471, 647
BmiI GGNNCC 1 cut(s) 473
BmsI GCATC 3 cut(s) 35, 388, 410
BpmI CTGGAG 1 cut(s) 97
Bpu1102I GCTNAGC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bse1I ACTGG 1 cut(s) 114
Bse3DI GCAATG 2 cut(s) 213, 336
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 3 cut(s) 50, 403, 649
BseMI GCAATG 2 cut(s) 213, 336
BseMII CTCAG 3 cut(s) 108, 356, 653
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 190, 266
BshFI GGCC 3 cut(s) 110, 263, 472
BsmAI GTCTC 1 cut(s) 17
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 3 cut(s) 110, 263, 472
Bsp143I GATC 2 cut(s) 193, 370
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 1 cut(s) 154
BspANI GGCC 3 cut(s) 110, 263, 472
BspCNI CTCAG 3 cut(s) 107, 355, 652
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 213, 336
BsrI ACTGG 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 2 cut(s) 193, 370
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 2 cut(s) 314, 536
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 3 cut(s) 94, 342, 639
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 3 cut(s) 50, 403, 649
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 2 cut(s) 196, 373
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 193, 370
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 190, 266
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 3 cut(s) 110, 263, 472
BtgI CCRYGG 1 cut(s) 154
BtsCI GGATG 3 cut(s) 50, 403, 649
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 2 cut(s) 471, 647
CseI GACGC 1 cut(s) 524
Csp6I GTAC 3 cut(s) 315, 453, 661
CviAII CATG 2 cut(s) 155, 632
CviQI GTAC 3 cut(s) 315, 453, 661
DdeI CTNAG 3 cut(s) 94, 342, 639
DpnI GATC 2 cut(s) 195, 372
DpnII GATC 2 cut(s) 193, 370
Eco130I CCWWGG 1 cut(s) 154
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 647
Eco47III AGCGCT 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 471
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 158, 635
FaiI YATR 9 cut(s) 41, 65, 156, 186, 323, 532, 545, 633, 665
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FatI CATG 2 cut(s) 154, 631
Fnu4HI GCNGC 2 cut(s) 204, 280
FokI GGATG 3 cut(s) 57, 410, 656
Fsp4HI GCNGC 2 cut(s) 204, 280
FspBI CTAG 3 cut(s) 29, 71, 131
GlaI GCGC 1 cut(s) 37
GluI GCNGC 2 cut(s) 204, 280
GsuI CTGGAG 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 3 cut(s) 110, 263, 472
HgaI GACGC 1 cut(s) 524
HhaI GCGC 1 cut(s) 38
Hin1II CATG 2 cut(s) 158, 635
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 25, 635
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 6 cut(s) 7, 22, 97, 123, 225, 438
Hpy188III TCNNGA 5 cut(s) 29, 173, 539, 632, 641
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 244, 373
HpyCH4III ACNGT 2 cut(s) 314, 536
HpyCH4V TGCA 6 cut(s) 206, 211, 282, 401, 560, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 3 cut(s) 94, 342, 639
Hsp92II CATG 2 cut(s) 158, 635
HspAI GCGC 1 cut(s) 36
Kzo9I GATC 2 cut(s) 193, 370
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 8 cut(s) 90, 127, 186, 192, 335, 488, 626, 643
Lsp1109I GCAGC 2 cut(s) 190, 266
LweI GCATC 3 cut(s) 35, 388, 410
MaeI CTAG 3 cut(s) 29, 71, 131
MaeIII GTNAC 1 cut(s) 512
MalI GATC 2 cut(s) 195, 372
MboI GATC 2 cut(s) 193, 370
MboII GAAGA 2 cut(s) 20, 490
MflI RGATCY 1 cut(s) 193
MluCI AATT 2 cut(s) 51, 164
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 5 cut(s) 121, 322, 388, 453, 508
Mph1103I ATGCAT 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 7 cut(s) 150, 242, 258, 377, 615, 621, 673
Mva1269I GAATGC 1 cut(s) 608
MwoI GCNNNNNNNGC 1 cut(s) 269
NcoI CCATGG 1 cut(s) 154
NdeII GATC 2 cut(s) 193, 370
NlaIII CATG 2 cut(s) 158, 635
NlaIV GGNNCC 1 cut(s) 473
NmeAIII GCCGAG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 403
PagI TCATGA 1 cut(s) 631
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 635
PkrI GCNGC 2 cut(s) 205, 281
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 473
PspPI GGNCC 2 cut(s) 471, 647
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 3 cut(s) 316, 454, 662
RsaNI GTAC 3 cut(s) 315, 453, 661
SaqAI TTAA 7 cut(s) 150, 242, 258, 377, 615, 621, 673
SatI GCNGC 2 cut(s) 204, 280
Sau3AI GATC 2 cut(s) 193, 370
Sau96I GGNCC 2 cut(s) 471, 647
SchI GAGTC 1 cut(s) 19
SetI ASST 8 cut(s) 132, 181, 205, 236, 274, 600, 621, 662
SfaNI GCATC 3 cut(s) 35, 388, 410
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 647
Sse9I AATT 2 cut(s) 51, 164
SspMI CTAG 3 cut(s) 29, 71, 131
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 2 cut(s) 314, 536
TasI AATT 2 cut(s) 51, 164
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 7 cut(s) 150, 242, 258, 377, 615, 621, 673
Tru9I TTAA 7 cut(s) 150, 242, 258, 377, 615, 621, 673
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 203, 279
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 3 cut(s) 201, 242, 620
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 1 cut(s) 164
XbaI TCTAGA 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 29, 71, 131
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.