FvH4_5g30120

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008218
Physical Location & Seq
Reverse (-)
21030310 .. 21036724
6415 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30120.t1

Sequence Viewer

Length: 1488 bp
ATGAAGCTCCTAATGGAGGAAATCAAACAAAGTGATGGTCAAGTTATTCTGTTTATCGATGAGATGCACAATTTAATGCAAACAGGAAACCGATCATCAGAAGAGGGGGGAGGAATTGATGGTGCAAATTTATTGAAGCCGGCTCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACGCTAGATGAATACAGAAAGTATATTGAAAAGGACCCAGCATTTGAAAGAAGATTCCAACCGGTCCAAATTCCTGAACCAACTGTGGATGAAACCATACACATTTTGAGAGGGCTTCGACAACGATATGAGATCCACCATCAAGTCCAGTATACAGATGAAGCCCTGAAAGCAGCAGCGCAGCTATCTCACCGATACATCAGCGATCGGTTTCTTCCTGATAAAGCAATTGACTTCATCGATGATGCTGGTTCTGCATTAAGACTGCATCGTCCTGCACAGCTTCCTAAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAACTGAAGAATGAAGCTATTCATGGACAAGAGTTTGAGAAGGCTTCACTATTACGTGACGCAGAATTGGATCTTAAGACACAGATATGTGAGGCATTGGATAATGTTAATGGTGGAAGTACTGTTACTGAATTGGATATTCAACACTGTGTGTCCCGTGGGACTGGTATTCCGGTTGAGAAAGTGTCAACAAATGAAGCCGATCGCCTCCTCAAGATGGAAGAGACGCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAGGGCTATTAGCAGAGCTATTCGTCGAGCTCGTGTTGGACTGAAGAATCCTAATAGGCCAATTGCCAGTTTCATCTTTTGTGGTCCGACTGGTGTTGGAAAATCTGAGCTCGCGAAAACTTTGGCGACTGGCTATTATGGCTCTGAAAAATCAATGGTGAGGCTTGATATGAGTGAGTTCATGGAACACCTTTCTGTTTCCAAGCTCATTGGTGCTCCCCCTGGTTATATTGGTTACTACGAAGGCGGTCAGCTGACTGAAGCCGTTCGTCGATGTCCTTACAGTCTGGTGCTTTTTGACGAGATTGAGAAGGCTCATCCTGACATTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTATAGATTTCAAGAATACACTAATCATTATGACGTCTAATGTTGGAAGCAGTGACATTGAGAACAAGGATGGCAATTACAAACAAATTAAGGGCTCGGTGACAGAGAAATTGAAACACCACTTTCGACCAGAGTTTTTAAATAGGTTGGATGAAACAATTGTTTTTCAGCAGCTGACTAAGTTGGAGGTAAAGGAAATTGCAGATATAATGCTGAAGGAGTTTCATGATAAGCTCAAGTCCAAAGAGATAAAGCTTCGGGTGACAGAAAGGTTCAAAGACAAAGTGGTTGACGAAGGATACGACCAAGCTATGGAGCAAGGCCTTTAA

Protein Analysis

496

Amino Acids

56.09

Weight (kDa)

5.87

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_9 PF17871 89 - 190 1.2e-31 AAA lid domain
Sigma54_activat PF00158 275 - 386 3e-06 Sigma-54 interaction domain
AAA_2 PF07724 277 - 438 4.9e-66 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 282 - 403 3.8e-11 AAA domain (dynein-related subfamily)
ClpB_D2-small PF10431 445 - 491 1.4e-07 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 450
AatII GACGTC 1 cut(s) 1196
AccB7I CCANNNNNTGG 1 cut(s) 1471
AccI GTMKAC 1 cut(s) 332
AccII CGCG 1 cut(s) 887
AciI CCGC 2 cut(s) 748, 1020
AclWI GGATC 2 cut(s) 307, 588
AcsI RAATTY 2 cut(s) 127, 249
AcuI CTGAAG 4 cut(s) 536, 836, 1053, 1394
AcyI GRCGYC 1 cut(s) 1193
AdeI CACNNNGTG 1 cut(s) 661
AfaI GTAC 1 cut(s) 631
AfiI CCNNNNNNNGG 4 cut(s) 16, 727, 748, 1471
AflII CTTAAG 1 cut(s) 584
AgeI ACCGGT 1 cut(s) 241
AgsI TTSAA 9 cut(s) 136, 209, 227, 653, 1105, 1118, 1171, 1273, 1435
AjnI CCWGG 1 cut(s) 994
Alw21I GWGCWC 3 cut(s) 805, 885, 991
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 2 cut(s) 307, 588
AlwNI CAGNNNCTG 1 cut(s) 1333
AoxI GGCC 2 cut(s) 830, 1480
ApeKI GCWGC 4 cut(s) 353, 356, 361, 1330
ApoI RAATTY 2 cut(s) 127, 249
ArsI GACNNNNNNTTYG 4 cut(s) 22, 54, 206, 238
AsiGI ACCGGT 1 cut(s) 241
Asp700I GAANNNNTTC 2 cut(s) 528, 1038
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 3 cut(s) 214, 244, 857
AsuHPI GGTGA 5 cut(s) 167, 362, 943, 1270, 1432
AvaII GGWCC 3 cut(s) 214, 244, 857
BanII GRGCYC 3 cut(s) 805, 885, 1256
BarI GAAGNNNNNNTAC 2 cut(s) 619, 651
BauI CACGAG 1 cut(s) 804
Bbv12I GWGCWC 3 cut(s) 805, 885, 991
BbvI GCAGC 4 cut(s) 365, 368, 373, 1342
BccI CCATC 6 cut(s) 29, 113, 327, 721, 1124, 1223
BceAI ACGGC 1 cut(s) 1022
BciT130I CCWGG 1 cut(s) 996
BciVI GTATCC 1 cut(s) 1451
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 161, 1161
BfrI CTTAAG 1 cut(s) 584
BfuI GTATCC 1 cut(s) 1451
BisI GCNGC 4 cut(s) 354, 357, 362, 1331
BlsI GCNGC 4 cut(s) 355, 358, 363, 1332
BmcAI AGTACT 1 cut(s) 631
Bme1390I CCNGG 1 cut(s) 996
Bme18I GGWCC 3 cut(s) 214, 244, 857
BmgT120I GGNCC 3 cut(s) 214, 244, 857
BmiI GGNNCC 2 cut(s) 175, 216
BmrFI CCNGG 1 cut(s) 996
BmsI GCATC 4 cut(s) 54, 415, 457, 1101
BpuEI CTTGAG 4 cut(s) 462, 707, 1145, 1379
Bsa29I ATCGAT 2 cut(s) 57, 420
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 1193
BsaJI CCNNGG 2 cut(s) 667, 994
BsaWI WCCGGW 2 cut(s) 241, 682
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 727, 748, 1471
Bse118I RCCGGY 2 cut(s) 139, 241
Bse1I ACTGG 5 cut(s) 328, 679, 840, 868, 907
BseBI CCWGG 1 cut(s) 996
BseCI ATCGAT 2 cut(s) 57, 420
BseDI CCNNGG 2 cut(s) 667, 994
BseGI GGATG 6 cut(s) 274, 769, 1090, 1135, 1234, 1315
BseLI CCNNNNNNNGG 4 cut(s) 16, 727, 748, 1471
BseMII CTCAG 2 cut(s) 488, 870
BseNI ACTGG 5 cut(s) 328, 679, 840, 868, 907
BseRI GAGGAG 1 cut(s) 710
BseXI GCAGC 4 cut(s) 365, 368, 373, 1342
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 887
Bsh1285I CGRYCG 2 cut(s) 388, 715
BshFI GGCC 2 cut(s) 832, 1482
BshTI ACCGGT 1 cut(s) 241
BshVI ATCGAT 2 cut(s) 57, 420
BsiEI CGRYCG 2 cut(s) 388, 715
BsiHKAI GWGCWC 3 cut(s) 805, 885, 991
BsiSI CCGG 3 cut(s) 140, 242, 683
BslFI GGGAC 2 cut(s) 649, 685
BslI CCNNNNNNNGG 4 cut(s) 16, 727, 748, 1471
BsmAI GTCTC 1 cut(s) 728
BsmBI CGTCTC 1 cut(s) 728
BsmFI GGGAC 2 cut(s) 649, 685
BsnI GGCC 2 cut(s) 832, 1482
Bsp1286I GDGCHC 4 cut(s) 805, 885, 991, 1256
Bsp143I GATC 5 cut(s) 92, 312, 385, 580, 712
Bsp68I TCGCGA 1 cut(s) 887
BspACI CCGC 2 cut(s) 748, 1020
BspANI GGCC 2 cut(s) 832, 1482
BspCNI CTCAG 2 cut(s) 489, 871
BspDI ATCGAT 2 cut(s) 57, 420
BspFNI CGCG 1 cut(s) 887
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 2 cut(s) 175, 216
BspMAI CTGCAG 1 cut(s) 165
BspPI GGATC 2 cut(s) 307, 588
BspTI CTTAAG 1 cut(s) 584
BsrFI RCCGGY 2 cut(s) 139, 241
BsrI ACTGG 5 cut(s) 328, 679, 840, 868, 907
BssAI RCCGGY 2 cut(s) 139, 241
BssECI CCNNGG 2 cut(s) 667, 994
BssMI GATC 5 cut(s) 92, 312, 385, 580, 712
BssNAI GTATAC 1 cut(s) 333
BssNI GRCGYC 1 cut(s) 1193
BssSI CACGAG 1 cut(s) 804
Bst1107I GTATAC 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 4 cut(s) 265, 634, 659, 1058
Bst6I CTCTTC 2 cut(s) 96, 726
BstACI GRCGYC 1 cut(s) 1193
BstAFI CTTAAG 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 141, 885
BstDEI CTNAG 3 cut(s) 497, 879, 1338
BstDSI CCRYGG 1 cut(s) 667
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 6 cut(s) 274, 769, 1090, 1135, 1234, 1315
BstFNI CGCG 1 cut(s) 887
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 5 cut(s) 95, 315, 388, 583, 715
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 5 cut(s) 92, 312, 385, 580, 712
BstMCI CGRYCG 2 cut(s) 388, 715
BstMWI GCNNNNNNNGC 5 cut(s) 350, 434, 745, 776, 912
BstNI CCWGG 1 cut(s) 996
BstSCI CCNGG 1 cut(s) 994
BstSFI CTRYAG 2 cut(s) 161, 1161
BstUI CGCG 1 cut(s) 887
BstV1I GCAGC 4 cut(s) 365, 368, 373, 1342
BstX2I RGATCY 2 cut(s) 312, 580
BstYI RGATCY 2 cut(s) 312, 580
BstZ17I GTATAC 1 cut(s) 333
Bsu15I ATCGAT 2 cut(s) 57, 420
BsuI GTATCC 1 cut(s) 1451
BsuRI GGCC 2 cut(s) 832, 1482
BsuTUI ATCGAT 2 cut(s) 57, 420
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 6 cut(s) 274, 769, 1090, 1135, 1234, 1315
BtsI GCAGTG 2 cut(s) 170, 1216
BtsIMutI CAGTG 3 cut(s) 170, 655, 1216
BtuMI TCGCGA 1 cut(s) 887
Cac8I GCNNGC 2 cut(s) 141, 885
CaiI CAGNNNCTG 1 cut(s) 1333
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 2 cut(s) 139, 241
Cfr13I GGNCC 3 cut(s) 214, 244, 857
ClaI ATCGAT 2 cut(s) 57, 420
CseI GACGC 2 cut(s) 578, 745
Csp6I GTAC 1 cut(s) 630
CspAI ACCGGT 1 cut(s) 241
CviAII CATG 3 cut(s) 533, 955, 1385
CviQI GTAC 1 cut(s) 630
DdeI CTNAG 3 cut(s) 497, 879, 1338
DpnI GATC 5 cut(s) 94, 314, 387, 582, 714
DpnII GATC 5 cut(s) 92, 312, 385, 580, 712
DraI TTTAAA 1 cut(s) 1299
DraIII CACNNNGTG 1 cut(s) 661
DrdI GACNNNNNNGTC 1 cut(s) 450
DseDI GACNNNNNNGTC 1 cut(s) 450
Eam1104I CTCTTC 2 cut(s) 96, 726
EarI CTCTTC 2 cut(s) 96, 726
Ecl136II GAGCTC 2 cut(s) 803, 883
Eco147I AGGCCT 1 cut(s) 1482
Eco24I GRGCYC 3 cut(s) 805, 885, 1256
Eco47I GGWCC 3 cut(s) 214, 244, 857
Eco53kI GAGCTC 2 cut(s) 803, 883
Eco57I CTGAAG 4 cut(s) 536, 836, 1053, 1394
EcoICRI GAGCTC 2 cut(s) 803, 883
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 994
EcoT38I GRGCYC 3 cut(s) 805, 885, 1256
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 3 cut(s) 536, 958, 1388
FaqI GGGAC 2 cut(s) 649, 685
FatI CATG 3 cut(s) 532, 954, 1384
FauI CCCGC 1 cut(s) 741
FblI GTMKAC 1 cut(s) 332
Fnu4HI GCNGC 4 cut(s) 354, 357, 362, 1331
FokI GGATG 6 cut(s) 281, 776, 1077, 1142, 1241, 1322
FriOI GRGCYC 3 cut(s) 805, 885, 1256
Fsp4HI GCNGC 4 cut(s) 354, 357, 362, 1331
FspBI CTAG 1 cut(s) 185
GlaI GCGC 1 cut(s) 360
GluI GCNGC 4 cut(s) 354, 357, 362, 1331
GsaI CCCAGC 1 cut(s) 221
HaeIII GGCC 2 cut(s) 832, 1482
HapII CCGG 3 cut(s) 140, 242, 683
HgaI GACGC 2 cut(s) 578, 745
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 1193
Hin1II CATG 3 cut(s) 536, 958, 1388
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 2 cut(s) 699, 1450
HindII GTYRAC 2 cut(s) 699, 1450
HindIII AAGCTT 1 cut(s) 1412
HinfI GANTC 2 cut(s) 234, 820
HpaII CCGG 3 cut(s) 140, 242, 683
HphI GGTGA 5 cut(s) 167, 362, 943, 1270, 1432
Hpy166II GTNNAC 4 cut(s) 158, 333, 699, 1450
Hpy188I TCNGA 5 cut(s) 100, 498, 861, 880, 919
Hpy188III TCNNGA 9 cut(s) 254, 398, 479, 724, 761, 886, 1094, 1171, 1385
Hpy8I GTNNAC 4 cut(s) 158, 333, 699, 1450
Hpy99I CGWCG 2 cut(s) 801, 1047
HpyAV CCTTC 5 cut(s) 544, 1010, 1078, 1369, 1448
HpyCH4III ACNGT 4 cut(s) 265, 634, 659, 1058
HpyCH4IV ACGT 2 cut(s) 565, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 350, 434, 745, 776, 912
HpyF3I CTNAG 3 cut(s) 497, 879, 1338
HpySE526I ACGT 2 cut(s) 565, 1193
Hsp92I GRCGYC 1 cut(s) 1193
Hsp92II CATG 3 cut(s) 536, 958, 1388
HspAI GCGC 1 cut(s) 359
KroI GCCGGC 1 cut(s) 139
KroNI GCCGGC 1 cut(s) 141
Kzo9I GATC 5 cut(s) 92, 312, 385, 580, 712
LmnI GCTCC 5 cut(s) 12, 173, 744, 994, 1474
Lsp1109I GCAGC 4 cut(s) 365, 368, 373, 1342
LweI GCATC 4 cut(s) 54, 415, 457, 1101
MaeI CTAG 1 cut(s) 185
MaeII ACGT 2 cut(s) 565, 1193
MaeIII GTNAC 6 cut(s) 566, 634, 1007, 1211, 1258, 1420
MalI GATC 5 cut(s) 94, 314, 387, 582, 714
MboI GATC 5 cut(s) 92, 312, 385, 580, 712
MboII GAAGA 7 cut(s) 113, 243, 386, 529, 743, 829, 1146
MfeI CAATTG 3 cut(s) 408, 834, 1317
MflI RGATCY 2 cut(s) 312, 580
MhlI GDGCHC 4 cut(s) 805, 885, 991, 1256
MmeI TCCRAC 7 cut(s) 262, 790, 850, 884, 1183, 1287, 1323
MroNI GCCGGC 1 cut(s) 139
MroXI GAANNNNTTC 2 cut(s) 528, 1038
MseI TTAA 7 cut(s) 74, 440, 585, 618, 1248, 1298, 1486
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 2 cut(s) 1027, 1333
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 3 cut(s) 140, 242, 683
MspR9I CCNGG 1 cut(s) 996
MunI CAATTG 3 cut(s) 408, 834, 1317
MvaI CCWGG 1 cut(s) 996
MvnI CGCG 1 cut(s) 887
MwoI GCNNNNNNNGC 5 cut(s) 350, 434, 745, 776, 912
NaeI GCCGGC 1 cut(s) 141
NdeII GATC 5 cut(s) 92, 312, 385, 580, 712
NgoMIV GCCGGC 1 cut(s) 139
NlaIII CATG 3 cut(s) 536, 958, 1388
NlaIV GGNNCC 2 cut(s) 175, 216
NmuCI GTSAC 4 cut(s) 566, 1211, 1258, 1420
NruI TCGCGA 1 cut(s) 887
PagI TCATGA 1 cut(s) 1384
PceI AGGCCT 1 cut(s) 1482
PcsI WCGNNNNNNNCGW 2 cut(s) 802, 1458
PdiI GCCGGC 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 528, 1038
PfeI GAWTC 2 cut(s) 234, 820
PflMI CCANNNNNTGG 1 cut(s) 1471
PinAI ACCGGT 1 cut(s) 241
PkrI GCNGC 4 cut(s) 355, 358, 363, 1332
Ple19I CGATCG 2 cut(s) 388, 715
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 214
Psp124BI GAGCTC 2 cut(s) 805, 885
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 994
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 2 cut(s) 175, 216
PspPI GGNCC 3 cut(s) 214, 244, 857
PspPPI RGGWCCY 1 cut(s) 214
PstI CTGCAG 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 1333
PsuI RGATCY 2 cut(s) 312, 580
PvuI CGATCG 2 cut(s) 388, 715
PvuII CAGCTG 2 cut(s) 1027, 1333
RruI TCGCGA 1 cut(s) 887
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
RseI CAYNNNNRTG 1 cut(s) 595
SacI GAGCTC 2 cut(s) 805, 885
SaqAI TTAA 7 cut(s) 74, 440, 585, 618, 1248, 1298, 1486
SatI GCNGC 4 cut(s) 354, 357, 362, 1331
Sau3AI GATC 5 cut(s) 92, 312, 385, 580, 712
Sau96I GGNCC 3 cut(s) 214, 244, 857
ScaI AGTACT 1 cut(s) 631
ScrFI CCNGG 1 cut(s) 996
SduI GDGCHC 4 cut(s) 805, 885, 991, 1256
SfaNI GCATC 4 cut(s) 54, 415, 457, 1101
SfcI CTRYAG 2 cut(s) 161, 1161
SinI GGWCC 3 cut(s) 214, 244, 857
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 5 cut(s) 477, 584, 722, 1124, 1394
SmoI CTYRAG 5 cut(s) 477, 584, 722, 1124, 1394
SseBI AGGCCT 1 cut(s) 1482
SsiI CCGC 2 cut(s) 748, 1020
SspI AATATT 1 cut(s) 493
SspMI CTAG 1 cut(s) 185
SstI GAGCTC 2 cut(s) 805, 885
StuI AGGCCT 1 cut(s) 1482
StyD4I CCNGG 1 cut(s) 994
TaaI ACNGT 4 cut(s) 265, 634, 659, 1058
TaiI ACGT 2 cut(s) 568, 1196
TaqI TCGA 6 cut(s) 57, 298, 420, 799, 1045, 1285
TatI WGTACW 1 cut(s) 629
TfiI GAWTC 2 cut(s) 234, 820
Tru1I TTAA 7 cut(s) 74, 440, 585, 618, 1248, 1298, 1486
Tru9I TTAA 7 cut(s) 74, 440, 585, 618, 1248, 1298, 1486
TscAI CASTG 3 cut(s) 170, 662, 1216
TseFI GTSAC 4 cut(s) 566, 1211, 1258, 1420
TseI GCWGC 4 cut(s) 353, 356, 361, 1330
Tsp45I GTSAC 4 cut(s) 566, 1211, 1258, 1420
TspRI CASTG 3 cut(s) 170, 662, 1216
Van91I CCANNNNNTGG 1 cut(s) 1471
Vha464I CTTAAG 1 cut(s) 584
VpaK11BI GGWCC 3 cut(s) 214, 244, 857
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 2 cut(s) 127, 249
XmiI GTMKAC 1 cut(s) 332
XmnI GAANNNNTTC 2 cut(s) 528, 1038
XspI CTAG 1 cut(s) 185
ZraI GACGTC 1 cut(s) 1194
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.