Rh4BG133500

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008218
Physical Location & Seq
Forward (+)
22418461 .. 22422676
4216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG133500.1

Sequence Viewer

Length: 2856 bp
ATGGCTAGGGTTTTGGCTCTGTCTTCAACAAATAATATTCCTTGTGTGGTTGGTGGGCTAGTTAGGACTGCTCAATCTAAAGAATTGGGTGGAAAAAAGAGTAGTAGAGTGAAGATGATGTGTACTTCTTCACTAGTAGGAGGAGCACTTGCTTTGAGAATGAGGTGCATGAGTAGTAGTAGCAGCACTAGTACTGCTTCTAGTTTCTCAGGACTCCGTAGGTTTAACGCCCTAGATAAGCTGGTGGCCACACCTAGCAGCCTACACCACGAGGAATACTCTTTTGGAATTGCAACCCCTTGGGGTTCCGAAAGTCGGAAGGGGGCTAGAAGTGGAAGAGCTAGCAGATGTGTACCTAAAGCCATGTTTGAGCGGTTCACAGACAAAGCAATTAAAGTGGTTCTGCTTGCACAAGAGGAATCAAGACGACTTGGTCACAATTTCGTGGGCACTGAGCAGATCCTTTTGGGTCTCATTGGTGAAGGAACTGGTCTTCCTTCCAAGGTTCTGAAATCCTCCATGGGAATTAATCTTAAAGAGGCACGAATTCAAGTGGAGAAGATAATTGGAAGGGGTTCTGGATTTGTTGCTATTGAGATCCCATTCACTTCTCGTGCCAAACGAGTTTTGGAACTCTCACTCGAAGAGGCTCGCCAACTTGGCCATAACTACATTGGATCCGAACACTTGCTTCTGGGACTGCTTCGAGAGGGGGAGGGTGTAGCAGCAGTTGTTCTAGCGAATTCTGGTGTCGATCCTTGTAATATTCGCACACAGGTTATCCATATGGTGGGCAGTGAGAACACAGCAGAAGCTGTTGTTGATCATAGGCGAGGGGATGATAAGACTAAAATGCCAACACTAGAGGAGTATGGGACCAATTTAACGAAGCTAGCAGAAGAGGGTAAGTTGGATCCTGTTGTTGGAAGAGAACAACAAATATCACGTGTCATCCAAATTTTGGGTAGGCGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCGATGCTCCAGAAACAATAGCCAAAAAGACGGTTATAACTCTTGATACGGGTCTTCTTGTGTCTGGTACCAAGTACCGCGGAGAGTTTGAGGGAAGATTGAAGTTGCTAATGGAGGAATTAAAAAAAAGTGGTGGTGAAGTTATTCTGTTTATCGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGAGCAATTGATGCTGCAAATTTATTGAAGCCGGCCCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACACTAGATGAATACAGAAAGCATATTGAAAAAGACCCAGCATTGGAAAGACGATTCCAACCAGTCAAAGTTCCTGAACCAACGGTGGATGAAACCATACAGGTTTTGAGAGGGCTTCGAGAACGATATGAGATTCACCACAAAGTGCAGTATACTGATGAGGCCCTGAAAGCAGCTGCGCAGCTAGCATACCAATACATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGCTCGATGAAGCTGGTTCTACAGTACGGCTCCGTCATGCACAGCTTCCTGAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAAGAGAAGAATGAAGCTAGTCGTAGCCAAGATTTTGAGAAGGCTGGGAAATTACGCGACAGAGAATTGGACCTTAAGACACAGATCTCAGCAGTAACAGAGAAAGGCAAGGAGATAAGTGAGGCATTGGATTATGTTAATGGTGGCCCTATTGTTACTGAATTGGATATTCAACATATTTTGTCTTGTTGGACTGGTATTCCAGTTGAGAAAGTGTCAACAAATGAATCTGATCGCCTCCTCAAGATGGAAGAGACGCTCCATAAGCGGGTTATTGGCCAGGATGAAGCAGTCAGGGCTATTAGCAGGGCTATTCGTCGAGCTCGTGTTGGACTGAAGAGTCCTAATCGGCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCTGAGCTCGCAAAAACTTTGGCGACTTGCTATTTTGGCTCTGAAGAATCAATGGTGAGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTGTTTCCAAGCTCATTGGTTCCCCCCCTGGTTATGTTGGTTACACAGAGGGCGGTCAGCTGGTCGAAGCCGTTCGTCGCCATCCATACACTCTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCTGATGTTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTGTAGATTTCAAGAATACACTCATCATTATGACGTCTAATGTTGGAAGCAGTGTCATTGAGAAAGGAGGCGGTAACATGGGATTTGACCCGGCCAAGGATGAAGACAAGGATAGCAGTTACAAACAAATTAAGAGCTTGGTGATGGAGGAATTGAAACACTACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATGATTGTTTTTCGGCAGCTGAGTAAGTTGGAGGTAAAGGAAATTGCAGATATAATGCTGAACGAGGTTTATGGGAGGCTCAAGTCCAAAGAGATAGAGCTTCGTGTGACAGAGAGGTTCAAAGACAAAGTGGTTGACGAAGGATACGACCCAAGCTATGGAGCAAGGCCTTTAAGAAGAGCCGTAATGAGACTTTTGGAGGACAGCATGGCTGAAAAGATGCTGGCAAGGGAGATCAAAGAGGGTGATTCAGTTATTGTGGATGTTGATTCTGATGGGAATGTGATTGTGCTCATCAATGGCAGCGCTAAGACAGAGATAAAGAAATAG

Protein Analysis

951

Amino Acids

104.95

Weight (kDa)

7.29

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 125 - 248 7.4e-29 Clp repeat (R) N-terminal domain
Clp_N PF02861 202 - 269 7.2e-15 Clp repeat (R) N-terminal domain
AAA_16 PF13191 306 - 352 9.8e-06 AAA ATPase domain
AAA PF00004 330 - 443 6.6e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 470 - 570 5.7e-34 AAA lid domain
UVR PF02151 544 - 578 3.8e-09 UvrB/uvrC motif
AAA_2 PF07724 667 - 842 4.3e-67 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 672 - 793 4.2e-11 AAA domain (dynein-related subfamily)
ClpB_D2-small PF10431 849 - 929 5.2e-25 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1094
AasI GACNNNNNNGTC 2 cut(s) 432, 1989
AatII GACGTC 1 cut(s) 2366
Acc16I TGCGCA 1 cut(s) 1503
Acc65I GGTACC 1 cut(s) 1124
AccB1I GGYRCC 1 cut(s) 1124
AccB7I CCANNNNNTGG 3 cut(s) 790, 961, 2684
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 1475
AccII CGCG 2 cut(s) 1137, 1707
AciI CCGC 7 cut(s) 373, 970, 1135, 1137, 1918, 2190, 2400
AclWI GGATC 7 cut(s) 454, 592, 672, 685, 749, 908, 921
AcoI YGGCCR 5 cut(s) 246, 661, 1927, 2000, 2421
AcsI RAATTY 4 cut(s) 546, 742, 957, 1270
AcuI CTGAAG 3 cut(s) 1629, 2006, 2109
AcvI CACGTG 1 cut(s) 947
AcyI GRCGYC 1 cut(s) 2363
AdeI CACNNNGTG 1 cut(s) 1468
AfaI GTAC 6 cut(s) 124, 193, 354, 1126, 1133, 1584
AfeI AGCGCT 1 cut(s) 2833
AflII CTTAAG 1 cut(s) 1724
AflIII ACRYGT 1 cut(s) 946
AhlI ACTAGT 2 cut(s) 133, 188
AjnI CCWGG 3 cut(s) 1003, 1929, 2164
AjuI GAANNNNNNNTTGG 4 cut(s) 267, 299, 993, 1025
AleI CACNNNNGTG 1 cut(s) 2231
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 5 cut(s) 148, 1224, 1975, 2055, 2820
Alw26I GTCTC 3 cut(s) 476, 1898, 2710
Alw44I GTGCAC 1 cut(s) 1220
AlwI GGATC 7 cut(s) 454, 592, 672, 685, 749, 908, 921
AlwNI CAGNNNCTG 1 cut(s) 815
Aor51HI AGCGCT 1 cut(s) 2833
AoxI GGCC 9 cut(s) 246, 661, 1284, 1485, 1795, 1927, 2000, 2421, 2693
ApaLI GTGCAC 1 cut(s) 1220
ApoI RAATTY 4 cut(s) 546, 742, 957, 1270
AseI ATTAAT 1 cut(s) 528
AsiSI GCGATCGC 1 cut(s) 1531
Asp700I GAANNNNTTC 3 cut(s) 574, 1200, 2208
Asp718I GGTACC 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 1504, 2834
AspS9I GGNCC 6 cut(s) 876, 1285, 1486, 1720, 1796, 2027
AsuC2I CCSGG 1 cut(s) 2420
AsuHPI GGTGA 7 cut(s) 491, 1205, 1310, 1451, 2113, 2482, 2783
AsuNHI GCTAGC 3 cut(s) 341, 892, 1507
AvaII GGWCC 3 cut(s) 876, 1720, 2027
BaeGI GKGCMC 2 cut(s) 452, 1224
BalI TGGCCA 3 cut(s) 248, 663, 1929
BamHI GGATCC 2 cut(s) 677, 913
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 2 cut(s) 1975, 2055
BauI CACGAG 3 cut(s) 269, 612, 1974
BbrPI CACGTG 1 cut(s) 947
BbsI GAAGAC 5 cut(s) 15, 485, 1022, 1103, 2439
Bbv12I GWGCWC 5 cut(s) 148, 1224, 1975, 2055, 2820
BccI CCATC 5 cut(s) 1891, 2226, 2294, 2467, 2795
BceAI ACGGC 3 cut(s) 1601, 2192, 2693
BciT130I CCWGG 3 cut(s) 1005, 1931, 2166
BciVI GTATCC 1 cut(s) 2663
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 1 cut(s) 2420
BcoDI GTCTC 3 cut(s) 476, 1898, 2710
BcuI ACTAGT 2 cut(s) 133, 188
BfmI CTRYAG 3 cut(s) 1304, 1578, 2331
BfoI RGCGCY 1 cut(s) 2835
BfrI CTTAAG 1 cut(s) 1724
BfuI GTATCC 1 cut(s) 2663
BglI GCCNNNNNGGC 1 cut(s) 660
BglII AGATCT 1 cut(s) 1734
BmcAI AGTACT 1 cut(s) 193
Bme1390I CCNGG 4 cut(s) 1005, 1931, 2166, 2420
Bme18I GGWCC 3 cut(s) 876, 1720, 2027
BmgT120I GGNCC 6 cut(s) 876, 1285, 1486, 1720, 1796, 2027
BmiI GGNNCC 8 cut(s) 307, 679, 877, 915, 1126, 1318, 1589, 2158
BmrFI CCNGG 4 cut(s) 1005, 1931, 2166, 2420
BmsI GCATC 4 cut(s) 1051, 1252, 2271, 2736
BmtI GCTAGC 3 cut(s) 345, 896, 1511
BpiI GAAGAC 5 cut(s) 15, 485, 1022, 1103, 2439
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 1 cut(s) 1050
BpuEI CTTGAG 4 cut(s) 1602, 1877, 2315, 2591
BpuMI CCSGG 1 cut(s) 2420
Bsa29I ATCGAT 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 947
BsaHI GRCGYC 1 cut(s) 2363
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 6 cut(s) 299, 501, 519, 1135, 2164, 2424
Bse118I RCCGGY 1 cut(s) 1282
Bse1I ACTGG 6 cut(s) 493, 1385, 1849, 1853, 2010, 2038
BseBI CCWGG 3 cut(s) 1005, 1931, 2166
BseCI ATCGAT 1 cut(s) 1212
BseDI CCNNGG 6 cut(s) 299, 501, 519, 1135, 2164, 2424
BseMII CTCAG 6 cut(s) 222, 444, 1628, 1752, 2040, 2537
BseNI ACTGG 6 cut(s) 493, 1385, 1849, 1853, 2010, 2038
BseRI GAGGAG 3 cut(s) 156, 881, 1880
BseSI GKGCMC 2 cut(s) 452, 1224
BseYI CCCAGC 2 cut(s) 1360, 1694
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 2 cut(s) 1137, 1707
Bsh1285I CGRYCG 1 cut(s) 1531
BshFI GGCC 9 cut(s) 248, 663, 1286, 1487, 1797, 1929, 2002, 2423, 2695
BshNI GGYRCC 1 cut(s) 1124
BshVI ATCGAT 1 cut(s) 1212
BsiEI CGRYCG 1 cut(s) 1531
BsiHKAI GWGCWC 5 cut(s) 148, 1224, 1975, 2055, 2820
BsiSI CCGG 2 cut(s) 1283, 2420
BslFI GGGAC 2 cut(s) 711, 889
BsmAI GTCTC 3 cut(s) 476, 1898, 2710
BsmBI CGTCTC 1 cut(s) 1898
BsmFI GGGAC 2 cut(s) 711, 889
BsnI GGCC 9 cut(s) 248, 663, 1286, 1487, 1797, 1929, 2002, 2423, 2695
Bso31I GGTCTC 1 cut(s) 476
Bsp1286I GDGCHC 6 cut(s) 148, 452, 1224, 1975, 2055, 2820
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 7 cut(s) 373, 970, 1135, 1137, 1918, 2190, 2400
BspANI GGCC 9 cut(s) 248, 663, 1286, 1487, 1797, 1929, 2002, 2423, 2695
BspCNI CTCAG 6 cut(s) 221, 445, 1629, 1751, 2041, 2538
BspDI ATCGAT 1 cut(s) 1212
BspFNI CGCG 2 cut(s) 1137, 1707
BspLI GGNNCC 8 cut(s) 307, 679, 877, 915, 1126, 1318, 1589, 2158
BspMAI CTGCAG 1 cut(s) 1308
BspOI GCTAGC 3 cut(s) 345, 896, 1511
BspPI GGATC 7 cut(s) 454, 592, 672, 685, 749, 908, 921
BspQI GCTCTTC 2 cut(s) 331, 2698
BspT107I GGYRCC 1 cut(s) 1124
BspTI CTTAAG 1 cut(s) 1724
BspTNI GGTCTC 1 cut(s) 476
BsrBI CCGCTC 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 1282
BsrI ACTGG 6 cut(s) 493, 1385, 1849, 1853, 2010, 2038
BssAI RCCGGY 1 cut(s) 1282
BssECI CCNNGG 6 cut(s) 299, 501, 519, 1135, 2164, 2424
BssNAI GTATAC 1 cut(s) 1476
BssNI GRCGYC 1 cut(s) 2363
BssSI CACGAG 3 cut(s) 269, 612, 1974
BssT1I CCWWGG 4 cut(s) 299, 501, 519, 2424
Bst1107I GTATAC 1 cut(s) 1476
Bst2BI CACGAG 3 cut(s) 269, 612, 1974
Bst2UI CCWGG 3 cut(s) 1005, 1931, 2166
Bst4CI ACNGT 5 cut(s) 1090, 1408, 1582, 2140, 2332
Bst6I CTCTTC 7 cut(s) 331, 639, 894, 922, 1896, 1982, 2698
BstACI GRCGYC 1 cut(s) 2363
BstAFI CTTAAG 1 cut(s) 1724
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstBAI YACGTR 1 cut(s) 947
BstC8I GCNNGC 8 cut(s) 343, 408, 652, 894, 1284, 1509, 2055, 2751
BstDEI CTNAG 8 cut(s) 208, 453, 975, 1637, 1738, 2049, 2546, 2835
BstDSI CCRYGG 2 cut(s) 519, 1135
BstFNI CGCG 2 cut(s) 1137, 1707
BstH2I RGCGCY 1 cut(s) 2835
BstHHI GCGC 2 cut(s) 1504, 2834
BstMAI GTCTC 3 cut(s) 476, 1898, 2710
BstMCI CGRYCG 1 cut(s) 1531
BstMWI GCNNNNNNNGC 9 cut(s) 660, 1244, 1253, 1262, 1493, 1508, 1915, 1946, 2082
BstNI CCWGG 3 cut(s) 1005, 1931, 2166
BstSCI CCNGG 4 cut(s) 1003, 1929, 2164, 2418
BstSFI CTRYAG 3 cut(s) 1304, 1578, 2331
BstSLI GKGCMC 2 cut(s) 452, 1224
BstUI CGCG 2 cut(s) 1137, 1707
BstV2I GAAGAC 5 cut(s) 15, 485, 1022, 1103, 2439
BstX2I RGATCY 5 cut(s) 459, 597, 677, 913, 1734
BstYI RGATCY 5 cut(s) 459, 597, 677, 913, 1734
BstZ17I GTATAC 1 cut(s) 1476
Bsu15I ATCGAT 1 cut(s) 1212
BsuI GTATCC 1 cut(s) 2663
BsuRI GGCC 9 cut(s) 248, 663, 1286, 1487, 1797, 1929, 2002, 2423, 2695
BsuTUI ATCGAT 1 cut(s) 1212
BtgI CCRYGG 2 cut(s) 519, 1135
BtgZI GCGATG 1 cut(s) 1074
BtsI GCAGTG 3 cut(s) 802, 1313, 2386
BtsIMutI CAGTG 5 cut(s) 450, 802, 1313, 2136, 2386
Cac8I GCNNGC 8 cut(s) 343, 408, 652, 894, 1284, 1509, 2055, 2751
CaiI CAGNNNCTG 1 cut(s) 815
CfoI GCGC 2 cut(s) 1504, 2834
Cfr10I RCCGGY 1 cut(s) 1282
Cfr13I GGNCC 6 cut(s) 876, 1285, 1486, 1720, 1796, 2027
Cfr42I CCGCGG 1 cut(s) 1138
ClaI ATCGAT 1 cut(s) 1212
CseI GACGC 1 cut(s) 1915
CsiI ACCWGGT 1 cut(s) 1003
Csp6I GTAC 6 cut(s) 123, 192, 353, 1125, 1132, 1583
CspCI CAANNNNNGTGG 2 cut(s) 378, 413
CviAII CATG 7 cut(s) 169, 364, 520, 1595, 2125, 2407, 2734
CviQI GTAC 6 cut(s) 123, 192, 353, 1125, 1132, 1583
DdeI CTNAG 8 cut(s) 208, 453, 975, 1637, 1738, 2049, 2546, 2835
DraIII CACNNNGTG 1 cut(s) 1468
DrdI GACNNNNNNGTC 2 cut(s) 432, 1989
DseDI GACNNNNNNGTC 2 cut(s) 432, 1989
EaeI YGGCCR 5 cut(s) 246, 661, 1927, 2000, 2421
Eam1104I CTCTTC 7 cut(s) 331, 639, 894, 922, 1896, 1982, 2698
EarI CTCTTC 7 cut(s) 331, 639, 894, 922, 1896, 1982, 2698
EciI GGCGGA 1 cut(s) 985
Ecl136II GAGCTC 2 cut(s) 1973, 2053
Eco130I CCWWGG 4 cut(s) 299, 501, 519, 2424
Eco147I AGGCCT 1 cut(s) 2695
Eco24I GRGCYC 2 cut(s) 1975, 2055
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 3 cut(s) 876, 1720, 2027
Eco47III AGCGCT 1 cut(s) 2833
Eco53kI GAGCTC 2 cut(s) 1973, 2053
Eco57I CTGAAG 3 cut(s) 1629, 2006, 2109
Eco72I CACGTG 1 cut(s) 947
EcoICRI GAGCTC 2 cut(s) 1973, 2053
EcoO109I RGGNCCY 1 cut(s) 1486
EcoRI GAATTC 2 cut(s) 546, 742
EcoRII CCWGG 3 cut(s) 1003, 1929, 2164
EcoT14I CCWWGG 4 cut(s) 299, 501, 519, 2424
EcoT38I GRGCYC 2 cut(s) 1975, 2055
ErhI CCWWGG 4 cut(s) 299, 501, 519, 2424
Esp3I CGTCTC 1 cut(s) 1898
FaeI CATG 7 cut(s) 172, 367, 523, 1598, 2128, 2410, 2737
FaqI GGGAC 2 cut(s) 711, 889
FatI CATG 7 cut(s) 168, 363, 519, 1594, 2124, 2406, 2733
FauI CCCGC 1 cut(s) 1911
FauNDI CATATG 1 cut(s) 786
FbaI TGATCA 1 cut(s) 823
FblI GTMKAC 1 cut(s) 1475
FriOI GRGCYC 2 cut(s) 1975, 2055
FspI TGCGCA 1 cut(s) 1503
GlaI GCGC 2 cut(s) 1503, 2833
GsaI CCCAGC 2 cut(s) 1364, 1698
GsuI CTGGAG 1 cut(s) 1050
HaeII RGCGCY 1 cut(s) 2835
HaeIII GGCC 9 cut(s) 248, 663, 1286, 1487, 1797, 1929, 2002, 2423, 2695
HapII CCGG 2 cut(s) 1283, 2420
HgaI GACGC 1 cut(s) 1915
HhaI GCGC 2 cut(s) 1504, 2834
Hin1I GRCGYC 1 cut(s) 2363
Hin1II CATG 7 cut(s) 172, 367, 523, 1598, 2128, 2410, 2737
Hin6I GCGC 2 cut(s) 1502, 2832
HinP1I GCGC 2 cut(s) 1502, 2832
HincII GTYRAC 2 cut(s) 1869, 2662
HindII GTYRAC 2 cut(s) 1869, 2662
HinfI GANTC 9 cut(s) 213, 419, 1377, 1456, 1877, 1990, 2093, 2774, 2795
HpaII CCGG 2 cut(s) 1283, 2420
HphI GGTGA 7 cut(s) 491, 1205, 1310, 1451, 2113, 2482, 2783
Hpy166II GTNNAC 8 cut(s) 123, 353, 378, 1222, 1301, 1476, 1869, 2662
Hpy188I TCNGA 9 cut(s) 310, 318, 510, 682, 1638, 1882, 2050, 2089, 2800
Hpy8I GTNNAC 8 cut(s) 123, 353, 378, 1222, 1301, 1476, 1869, 2662
Hpy99I CGWCG 2 cut(s) 1971, 2217
HpyAV CCTTC 9 cut(s) 313, 476, 507, 564, 1025, 1244, 1684, 2248, 2660
HpyCH4III ACNGT 5 cut(s) 1090, 1408, 1582, 2140, 2332
HpyCH4IV ACGT 3 cut(s) 946, 1045, 2363
HpyF10VI GCNNNNNNNGC 9 cut(s) 660, 1244, 1253, 1262, 1493, 1508, 1915, 1946, 2082
HpyF3I CTNAG 8 cut(s) 208, 453, 975, 1637, 1738, 2049, 2546, 2835
HpySE526I ACGT 3 cut(s) 946, 1045, 2363
Hsp92I GRCGYC 1 cut(s) 2363
Hsp92II CATG 7 cut(s) 172, 367, 523, 1598, 2128, 2410, 2737
HspAI GCGC 2 cut(s) 1502, 2832
KpnI GGTACC 1 cut(s) 1128
KroI GCCGGC 1 cut(s) 1282
KroNI GCCGGC 1 cut(s) 1284
Ksp22I TGATCA 1 cut(s) 823
KspI CCGCGG 1 cut(s) 1138
LguI GCTCTTC 2 cut(s) 331, 2698
LmnI GCTCC 9 cut(s) 143, 1069, 1235, 1241, 1253, 1316, 1593, 1914, 2687
LweI GCATC 4 cut(s) 1051, 1252, 2271, 2736
MabI ACCWGGT 1 cut(s) 1003
MaeII ACGT 3 cut(s) 946, 1045, 2363
MaeIII GTNAC 7 cut(s) 434, 1744, 1804, 2177, 2402, 2447, 2632
MbiI CCGCTC 1 cut(s) 373
MfeI CAATTG 4 cut(s) 1023, 1257, 1551, 2004
MflI RGATCY 5 cut(s) 459, 597, 677, 913, 1734
MhlI GDGCHC 6 cut(s) 148, 452, 1224, 1975, 2055, 2820
MlsI TGGCCA 3 cut(s) 248, 663, 1929
MluNI TGGCCA 3 cut(s) 248, 663, 1929
MlyI GAGTC 2 cut(s) 207, 1999
Mox20I TGGCCA 3 cut(s) 248, 663, 1929
MroNI GCCGGC 1 cut(s) 1282
MroXI GAANNNNTTC 3 cut(s) 574, 1200, 2208
MscI TGGCCA 3 cut(s) 248, 663, 1929
MslI CAYNNNNRTG 3 cut(s) 2231, 2265, 2357
Msp20I TGGCCA 3 cut(s) 248, 663, 1929
MspA1I CMGCKG 4 cut(s) 1137, 1499, 2197, 2545
MspCI CTTAAG 1 cut(s) 1724
MspI CCGG 2 cut(s) 1283, 2420
MspR9I CCNGG 4 cut(s) 1005, 1931, 2166, 2420
MunI CAATTG 4 cut(s) 1023, 1257, 1551, 2004
MvaI CCWGG 3 cut(s) 1005, 1931, 2166
MvnI CGCG 2 cut(s) 1137, 1707
MwoI GCNNNNNNNGC 9 cut(s) 660, 1244, 1253, 1262, 1493, 1508, 1915, 1946, 2082
NaeI GCCGGC 1 cut(s) 1284
NciI CCSGG 1 cut(s) 2420
NcoI CCATGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 786
NgoMIV GCCGGC 1 cut(s) 1282
NheI GCTAGC 3 cut(s) 341, 892, 1507
NlaIII CATG 7 cut(s) 172, 367, 523, 1598, 2128, 2410, 2737
NlaIV GGNNCC 8 cut(s) 307, 679, 877, 915, 1126, 1318, 1589, 2158
NmuCI GTSAC 2 cut(s) 434, 2632
NsbI TGCGCA 1 cut(s) 1503
OliI CACNNNNGTG 1 cut(s) 2231
PceI AGGCCT 1 cut(s) 2695
PciSI GCTCTTC 2 cut(s) 331, 2698
PcsI WCGNNNNNNNCGW 3 cut(s) 619, 1972, 2670
PdiI GCCGGC 1 cut(s) 1284
PdmI GAANNNNTTC 3 cut(s) 574, 1200, 2208
PfeI GAWTC 7 cut(s) 419, 1377, 1456, 1877, 2093, 2774, 2795
PflFI GACNNNGTC 1 cut(s) 432
PflMI CCANNNNNTGG 3 cut(s) 790, 961, 2684
Ple19I CGATCG 1 cut(s) 1531
PleI GAGTC 2 cut(s) 207, 1998
PmaCI CACGTG 1 cut(s) 947
PmlI CACGTG 1 cut(s) 947
PpsI GAGTC 2 cut(s) 207, 1998
Ppu21I YACGTR 1 cut(s) 947
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 1094
Psp124BI GAGCTC 2 cut(s) 1975, 2055
Psp6I CCWGG 3 cut(s) 1003, 1929, 2164
PspCI CACGTG 1 cut(s) 947
PspFI CCCAGC 2 cut(s) 1360, 1694
PspGI CCWGG 3 cut(s) 1003, 1929, 2164
PspN4I GGNNCC 8 cut(s) 307, 679, 877, 915, 1126, 1318, 1589, 2158
PspPI GGNCC 6 cut(s) 876, 1285, 1486, 1720, 1796, 2027
PstI CTGCAG 1 cut(s) 1308
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 5 cut(s) 459, 597, 677, 913, 1734
PsyI GACNNNGTC 1 cut(s) 432
PvuI CGATCG 1 cut(s) 1531
PvuII CAGCTG 3 cut(s) 1499, 2197, 2545
RgaI GCGATCGC 1 cut(s) 1531
RsaI GTAC 6 cut(s) 124, 193, 354, 1126, 1133, 1584
RsaNI GTAC 6 cut(s) 123, 192, 353, 1125, 1132, 1583
RseI CAYNNNNRTG 3 cut(s) 2231, 2265, 2357
SacI GAGCTC 2 cut(s) 1975, 2055
SacII CCGCGG 1 cut(s) 1138
SapI GCTCTTC 2 cut(s) 331, 2698
Sau96I GGNCC 6 cut(s) 876, 1285, 1486, 1720, 1796, 2027
ScaI AGTACT 1 cut(s) 193
SchI GAGTC 2 cut(s) 207, 1999
ScrFI CCNGG 4 cut(s) 1005, 1931, 2166, 2420
SduI GDGCHC 6 cut(s) 148, 452, 1224, 1975, 2055, 2820
SexAI ACCWGGT 1 cut(s) 1003
SfaAI GCGATCGC 1 cut(s) 1531
SfaNI GCATC 4 cut(s) 1051, 1252, 2271, 2736
SfcI CTRYAG 3 cut(s) 1304, 1578, 2331
Sfr303I CCGCGG 1 cut(s) 1138
SgfI GCGATCGC 1 cut(s) 1531
SgrBI CCGCGG 1 cut(s) 1138
SinI GGWCC 3 cut(s) 876, 1720, 2027
SmiMI CAYNNNNRTG 3 cut(s) 2231, 2265, 2357
SmlI CTYRAG 5 cut(s) 1617, 1724, 1892, 2294, 2606
SmoI CTYRAG 5 cut(s) 1617, 1724, 1892, 2294, 2606
SpeI ACTAGT 2 cut(s) 133, 188
SseBI AGGCCT 1 cut(s) 2695
SsiI CCGC 7 cut(s) 373, 970, 1135, 1137, 1918, 2190, 2400
SspI AATATT 3 cut(s) 37, 766, 1633
SstI GAGCTC 2 cut(s) 1975, 2055
StuI AGGCCT 1 cut(s) 2695
StyD4I CCNGG 4 cut(s) 1003, 1929, 2164, 2418
StyI CCWWGG 4 cut(s) 299, 501, 519, 2424
TaaI ACNGT 5 cut(s) 1090, 1408, 1582, 2140, 2332
TaiI ACGT 3 cut(s) 949, 1048, 2366
TaqI TCGA 9 cut(s) 642, 706, 753, 1212, 1441, 1563, 1969, 2202, 2497
TatI WGTACW 2 cut(s) 122, 191
TfiI GAWTC 7 cut(s) 419, 1377, 1456, 1877, 2093, 2774, 2795
TscAI CASTG 5 cut(s) 457, 802, 1313, 2143, 2386
TseFI GTSAC 2 cut(s) 434, 2632
Tsp45I GTSAC 2 cut(s) 434, 2632
TspGWI ACGGA 2 cut(s) 206, 1580
TspRI CASTG 5 cut(s) 457, 802, 1313, 2143, 2386
Tth111I GACNNNGTC 1 cut(s) 432
Van91I CCANNNNNTGG 3 cut(s) 790, 961, 2684
Vha464I CTTAAG 1 cut(s) 1724
VneI GTGCAC 1 cut(s) 1220
VpaK11BI GGWCC 3 cut(s) 876, 1720, 2027
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 4 cut(s) 546, 742, 957, 1270
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 671
XmiI GTMKAC 1 cut(s) 1475
XmnI GAANNNNTTC 3 cut(s) 574, 1200, 2208
ZraI GACGTC 1 cut(s) 2364
ZrmI AGTACT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.